Labshake search
Citations for Agilent :
4151 - 4200 of 5601 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... In order to generate the mutant expression plasmids for BMAL2 we used the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (210515, Agilent). The bHLH mutant was generated by converting an arginine to alanine (OaBMAL2_R88A ...
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bioRxiv - Microbiology 2021Quote: Site-directed mutagenesis of GFP-ORF57 for W292A mutant was carried out using QuikChange site-directed mutagenesis kit (Agilent technologies) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The fragment size estimation of the resulting libraries was assessed with High SensitivityTM HS DNA kit runned on 2100 Bioanalyzer (Agilent) and quantified using the QubitTM dsDNA High Sensitivity HS assay (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The fragment size estimation of the resulting libraries was assessed with High SensitivityTM HS DNA kit runned on 2100 Bioanalyzer (Agilent) and quantified using the QubitTM dsDNA High Sensitivity HS assay (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... the quality of RNA was first checked by assessing the RNA integrity number (RIN) using the Agilent RNA 6000 Nano kit (5067-1511, Agilent) and the Agilent 2100 Bioanalyzer Automated Analysis System (Agilent) ...
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bioRxiv - Plant Biology 2019Quote: ... A 600 ng aliquot of labelled cRNA was used for fragmentation and array loading (Gene Expression Hybridisation Kit, Agilent Technologies). Hybridization ...
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bioRxiv - Plant Biology 2020Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Pathology 2021Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent) according to the manufacturer’s instructions ...
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BCL-XL blockage in TNBC models confers vulnerability to inhibition of specific cell cycle regulatorsbioRxiv - Cancer Biology 2021Quote: ... RNA integrity was assessed using the Agilent RNA 6000 Pico kit and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Library quality and quantity was assessed on a Bioanalyzer using the DNA High Sensitivity Kit reagents and protocol (Agilent Technologies). The quantified libraries were pooled together at a final concentration of 100pM ...
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bioRxiv - Genetics 2021Quote: ... The HiC library was then hybridized with the custom capture baits and captured by the SureSelect XT HS and XT low input library preparation kit for ILM (Agilent). 15 Capture C libraries were made from 5 human primary melanocyte cultures (C56 ...
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bioRxiv - Pathology 2020Quote: ... RNA sample integrity was evaluated and concentration was measured using an Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent). Libraries for 16 samples were prepared using the Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit ...
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bioRxiv - Microbiology 2021Quote: All viral stocks were generated in Vero cells and tested negative for Mycoplasma contamination using a MycoSensor PCR Assay Kit (Agilent). Detailed passage histories of the ZIKV isolates used in this study were previously described (56) ...
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bioRxiv - Physiology 2021Quote: RNA was checked for quality using the Agilent RNA 600 nano kit and Bioanalyser instrument (Agilent Technologies, Santa Clara, CA). Aliquots of RNA (1 µg ...
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bioRxiv - Cancer Biology 2020Quote: ... GST-CD1 DENN V226K/I228D/L235D/Y238K and GST-CD1 DENN M243R/P244R/Q362A were generated using the QuickChange lightning site-directed mutagenesis kit from Agilent Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... and 1 μL was used to check the expected size (~300 bp) and the purify was verified with the Agilent High Sensitivity DNA Kit as manufacturer’s recommendation (Agilent Technologies). One μL of the diluted libraries were used to quantify as recommended in the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The final product was assessed for its size distribution and concentration using a BioAnalyzer High Sensitivity DNA Kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl (pH 8.5 ...
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bioRxiv - Pathology 2019Quote: Double immuno-enzymatic labelling of biopsies’ sections following the described pre-treatment was carried out by means of the EnVision peroxidase and alkaline phosphatase kits (DakoCytomation). Primary antibodies were incubated for 30 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Quality of total RNA was assessed on an Agilent BioAnalyzer 2100 using the RNA 6000 Nano kit (Agilent, # 5067-1511). All samples had a RIN > 7.6 ...
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bioRxiv - Cancer Biology 2021Quote: ... S200C and S200T) were introduced by site directed mutagenesis using QuikChange Lightning Site-Directed Mutagenesis Kit according to manufacturer’s protocol (Agilent Technologies). To express PRSS2 WT and Mutants protein ...
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bioRxiv - Cancer Biology 2019Quote: ... The quality of cDNA traces was assessed by using a High Sensitivity DNA Kit in a Bioanalyzer instrument (Agilent Technologies). Tagmentation and library preparation was performed using the Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2019Quote: E543M chiA-CTD mutant was created using pET46chiA-CTD template DNA with a QuikChange II Site-Directed Mutagenesis Kit (Stratagene) (Table S5) ...
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bioRxiv - Molecular Biology 2020Quote: ... and then analyzed by using the Agilent Tapestation 4200 System and High Sensitivity DNA D5000 kit (Agilent Technologies, CA, USA) to determine quality and quantity of the amplicons.
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bioRxiv - Neuroscience 2019Quote: ... A D1994N mutation was introduced into the pDC511-G2019S LRRK2 by site-directed mutagenesis using the QuickChange XL kit (Stratagene) as described (32) ...
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bioRxiv - Genetics 2021Quote: ... while the domain deletions were constructs from initial cDNA constructs using the site-directed mutagenesis kit QuikChange™ (Agilent Technologies) and adequate primers (Supplemental Table S4) ...
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bioRxiv - Plant Biology 2021Quote: ... RNA integrity was then assessed using Agilent 2100 Bioanalyzer with the RNA 6000 Nano Assay Kit (Agilent Technologies, CA, USA). Library preparation was carried out using Illumina TruSeq RNA sample preparation kit (Illumina ...
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bioRxiv - Plant Biology 2021Quote: ... and quality (RNA integrity > 8.5) of RNA was determined using a Bioanalyzer 2100 RNA 6000 Pico Total RNA Kit (Agilent Technologies). Samples containing >5 μg of RNA in total were used for RNAseq ...
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bioRxiv - Genomics 2021Quote: ... Final quality control of the purified RNAs was performed using the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip Kit (Agilent) and the Qubit Fluorometer with the Qubit RNA BR (Broad-Range ...
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bioRxiv - Microbiology 2021Quote: ... The specific variants were generated by site-directed mutagenesis in pCMV6-Entry-YTHDF2 using the QuikChange II site-Directed Mutagenesis Kit (Cat# 200521, Stratagene) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and Halo-V5-WTAP mutants (D301A and D301A/D302A) were generated using the QuikChange II site-directed Mutagenesis Kit (Stratagene) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... PartB RNA was quantified and purity/quality was assessed on the 2100 Agilent BioAnlayzer using the Agilent small RNA kit (5067-1548, Agilent).
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bioRxiv - Genetics 2020Quote: ... The A>T variant was introduced to PIK3C2B-pNL construct by site-directed mutagenesis using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) to generate PIK3C2B-A>T-pNL construct ...
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bioRxiv - Immunology 2019Quote: ... cDNA synthesis was performed using AffinityScript QPCR cDNA Synthesis kit following the manufacturer’s guidelines (Agilent Technologies, Santa Clara, CA, USA). The total reaction volume was 10 µL using 5 µL of Mastermix ...
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bioRxiv - Immunology 2019Quote: ... C-terminally HA-tagged wt IFITM3 genes in the lentiviral vector pHR-SIN cGGW PGK puro were mutagenized using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s instructions using the following primers (all listed 5’ to 3’) ...
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bioRxiv - Biochemistry 2019Quote: ... Mutagenesis was performed with pHmtS-ET22b as a template using a QuickChange site-directed mutagenesis kit (Stratagene, La Jolla, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Site-specific mutations of ACC2 and stop codon deletion constructs were performed using PCR-based mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent). The plasmids for C-terminus GST-tagged or Flag-tagged ACC2 fragments were re-cloned from the human ACC2 plasmid using PCR and Gibson assembly ...
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bioRxiv - Microbiology 2019Quote: ... All GBF1 truncations were introduced into the backbone of Venus tagged-GBF1/A795E (described in 57) using QuickChange XL site-directed mutagenesis kit from Agilent Technology ...
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bioRxiv - Developmental Biology 2019Quote: ... was created by introducing the point mutation in wild-type vasa cDNA sequence by site directed mutagenesis using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). The transgene is subsequently cloned downstream of GFP into the pUASK10attB vector (gift of Beat Suter) ...
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bioRxiv - Molecular Biology 2019Quote: ... Csl4-F121A and Csl4-F129A variants were generated by site-directed mutagenesis of their wildtype counterparts with appropriate sets of oligonucleotides using the QuikChange II XL Kit (Stratagene) as recommended.
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bioRxiv - Cell Biology 2019Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... RNA size and integrity were confirmed using RNA 6000 Pico kit for the Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) and Nanodrop™ ND-1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... vector encoding the pG gene as reported previously26 was site-specifically mutated by the insertion of an N-terminal serine using the QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent), according to the manufacturer’s instructions using following forward and reverse primers (ser codon underlined) ...
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bioRxiv - Genetics 2019Quote: ... Exons of DNA samples were captured using the in-solution enrichment methodology (Agilent SureSelect All Exon XT2 50 Mb Kit) and sequenced with an Illumina HiSeq 2500 instrument to generate 100 bp paired-end reads ...
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bioRxiv - Genomics 2019Quote: ... The prepared library was quantified using Qubit and validated for quality by running an aliquot (1 μl) on High Sensitivity DNA Kit (Agilent) which showed expected fragment distribution in the range of ~250–500 bp ...
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bioRxiv - Microbiology 2019Quote: ... Mutation of glycine at position 2 to alanine was accomplished using the Quick-Change Mutagenesis kit (Agilent, Santa Clara, CA) to generate ptubTgFBXO1HAG2A.
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bioRxiv - Genetics 2020Quote: ... WGS library-size selection was then verified using the 2100 Bioanalyzer DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA). The WGS library was then sequenced (2×150 bp ...
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bioRxiv - Genetics 2020Quote: ... in the nmt1-GFP-swi6+ expression construct (kindly gifted by Dr. Alison Pidoux) using the QuickChange site-directed mutagenesis kit (Stratagene), as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... plasmid using oligonucleotides containing the SHRF syndrome-linked G58V and G226D missense mutations and the QuikChange Site-Directed Mutagenesis Kit (Stratagene). The untagged RRP4/rrp4-G226D (pAC3656 ...
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bioRxiv - Genetics 2020Quote: The BRCA2 and PALB2 constructs were made siRNA resistant by introducing three silent mutations with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Site-directed mutagenesis of Aγ was performed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent, Santa Clara, CA). cRNAs were transcribed using mMessage mMachine Transcription Kits (Invitrogen) ...