Labshake search
Citations for Agilent :
4151 - 4200 of 4652 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: Fluorescence titrations were carried out at 25 °C using 1 × 0.3 cm path-length cuvettes in a Cary Eclipse spectrofluorimeter (Agilent Technologies, Waldbronn, Germany). Experiments were carried out in 20 mM K-phosphate ...
-
bioRxiv - Immunology 2022Quote: ... post-amplification and a 1:10 dilution of the resulting HTO libraries were run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess HTO quality and yield ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour at room temperature in the HRP-labelled polyclonal goat anti-mouse IgG (DAKO, cat. P0447) or HRP-labelled polyclonal goat anti-rabbit IgG (DAKO ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour at room temperature in the HRP-labelled polyclonal goat anti-rabbit IgG (DAKO, cat. P0448) or HRP-labelled polyclonal goat anti-mouse IgG (DAKO ...
-
bioRxiv - Cell Biology 2024Quote: Protein expression was initiated by transforming ∼80 ng of each DNA plasmid into 50 µL of XL-1 Chemically Supercompetent Cells (Agilent Technologies, 200236) at 42 °C for 30 seconds followed by 5 minutes of incubation on ice ...
-
bioRxiv - Immunology 2024Quote: ... The final PCR products were purified by AMPure XP beads at a 0.8:1 bead to sample ratio and quality controlled by TapeStation (D1000, Agilent, cat# 5067-5584). The samples were pooled at equimolar concentrations and further purified by gel extraction and AMPure XP bead clean up.
-
bioRxiv - Plant Biology 2024Quote: ... 1:500 cDNA dilutions were analyzed using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies, Santa Clara, CA, USA), required primer pairs (Table S2) ...
-
bioRxiv - Cancer Biology 2024Quote: ... at 1:400 for two hours and then incubated for 30 minutes with Rabbit Envision HRP System reagent (Agilent, Santa Clara, CA). For vimentin staining ...
-
bioRxiv - Immunology 2023Quote: ... An anti-rabbit IgG horseradish-peroxidase conjugated (1/2000 dilution) was used as a secondary antibody (Agilent DAKO, Santa Clara, CA, USA) and 4-Chloro-1-Naphthol as substrate for colorimetric detection.
-
bioRxiv - Microbiology 2023Quote: ... Fluorescence was measured at 50°C over 1 h at excitation 360 nm emission 460 nm on a BioTek Synergy HTX Multimode Reader (BioTek, Agilent, Tx, USA). The reaction rate was calculated as the maximum velocity over 12 sequential readings ...
-
bioRxiv - Synthetic Biology 2023Quote: ... composed of 1 ng of the cDNA library quantified using TapeStation with High Sensitivity D5000 ScreenTape (Agilent #5067-559 and #5067-5593), 0.7 µL each of 20 µM forward primer P5-TSO_Hybrid (45 ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were loaded onto a ZORBAX SB-C18 column (150 x 1 mm, 3.5 μm, flow of 90 μl/min; Agilent, Les Ulis, France) heated at 40°C ...
-
bioRxiv - Biochemistry 2022Quote: ... The metabolite-containing supernatant (195 µL) was transferred into pre-cut Bond® Elut PH (100 mg, 1 ml) SPE cartridges (Agilent, 12102005) placed in 1.7 mL microcentrifuge tubes and then centrifuged at ∼3000 g for 3 min ...
-
bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal rabbit anti-mouse secondary antibody IgG/HRP (P0260; Agilent Technologies/Dako) diluted 1:2000 in 3% BSA.
-
bioRxiv - Developmental Biology 2023Quote: Whole-mount immunostaining of differentiated skeletal muscle cells was performed using the monoclonal hybridoma 12/101 primary antibody [38] (1/200 dilution; DSHB #AB-531892) and the EnVision+ Mouse HRP kit (Agilent Technologies, K4007) according to the manufacturer’s recommendations.
-
bioRxiv - Microbiology 2023Quote: ... fitted with a flame photometric detector (FPD) and a J&W DB-1 column (30 m x 0.32 mm Ø; Agilent Technologies, USA). The oven temperature was 180°C ...
-
bioRxiv - Physiology 2023Quote: ... For β-cell proliferation analysis in P1 pups every other section was labeled with guinea pig anti-insulin (1:200; DAKO A0564) and mouse anti- BrdU (1:500 Sigma B2531 ...
-
Mass spectrometric analysis and biostimulatory effects of boar seminal gel, saliva and semen in pigsbioRxiv - Animal Behavior and Cognition 2023Quote: ... An aliquot of 1 μL was injected into GC-MS (GC 9000 and MS of G7077B, Agilent Technologies, Palo Alto, CA, USA) for further analysis ...
-
bioRxiv - Biophysics 2023Quote: ... the following HIV-1 IN mutants were prepared using the QuikChange II XL site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA): E138K ...
-
bioRxiv - Immunology 2023Quote: ... An anti-rabbit IgG horseradish-peroxidase conjugated (1/2000 dilution) was used as a secondary antibody (Agilent DAKO, Santa Clara, CA, USA) and 4-Chloro-1-Naphthol as substrate for colorimetric detection.
-
bioRxiv - Cancer Biology 2023Quote: ... depending on the antibody used (HIF-1α, Dako Target Retrieval Solution pH 6; for BACH-1, Dako Target Retrieval Solution pH 9). Next ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 100 μL of the ethyl acetate phase was transferred into a GC vial with insert and 1 μL was analyzed using GC 8890 (Agilent Technologies, USA) equipped with a flame ionization detector (FID ...
-
bioRxiv - Microbiology 2023Quote: ... Gas samples from the headspaces (250 μl) were collected using a 1 mL Hamilton’s SampleLock™ syringe and manually injected into a Gas Chromatograph (GC) (Agilent 7820A, AgilentTechnologies). H2 and O2 were separated using a packed column (60/80 Molecular Sieve 5A ...
-
bioRxiv - Developmental Biology 2023Quote: ... The quality and quantity of the amplified sequencing libraries were validated using a Fragment Analyzer SS NGS Fragment Kit (1–6000 bp) (Agilent, Waldbronn, Germany). The equimolar pool of samples was spiked into a NovaSeq6000 run targeting 20M reads per sample on a S1 FlowCell (Novaseq S1 Reagent Kit ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by site-directed mutagenesis at Cys322 site to Serine using QuikChange Site-directed mutagenesis kit (Agilent, Santa Clara, CA; Fig. 1). 4RCF construct (four microtubule-binding repeats and cysteine-free construct containing C291S and C322S mutations ...
-
bioRxiv - Genomics 2023Quote: ... The average library size was obtained via analysis of 1 μL of the library on the Agilent TapeStation 2200 (Agilent, Cat # G2964AA) using D1000 screen tape (Agilent ...
-
bioRxiv - Neuroscience 2024Quote: ... Levels of phosphorylated (pS396/404) tau were determined by a MSD sandwich ELISA using a polyclonal total tau antibody (DAKO, AA002402-1) as a capture antibody and a monoclonal pS396/404-tau antibody (PHF1 ...
-
bioRxiv - Neuroscience 2024Quote: ... A total of 1 × 105 cells per well were seeded on a 24-well Xfe96 Cell culture Microplate (Agilent, Cat# 100777-004) coated with poly-L-lysine (Millipore Sigma ...
-
bioRxiv - Pathology 2024Quote: ... One hour of shaking incubation at 30℃ resulted in the extraction of 1 mL of gas for ethylene measurement using gas chromatography (Agilent 7820A, USA).
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 90 minutes in a biotin conjugated secondary antibody (a goat anti-rabbit, a goat anti-mouse or a swine anti-rabbit as appropriate; Dako; diluted 1:500) and for 1h in a peroxidase conjugated extravidin (Sigma ...
-
bioRxiv - Immunology 2020Quote: ... Purified cDNA was subjected to quality control using 1 µl of cDNA on an Agilent 2100 BioAnalyser (Agilent Technologies, Santa Clara, CA, USA) using the Agilent High Sensitivity DNA kit ...
-
bioRxiv - Molecular Biology 2019Quote: ... concentration and purity were evaluated on an Agilent RNA6000 Nano Chip in Reagent Port 1 of the Bioanalyzer Agilent 2100 (Agilent Technologies, CA, USA). Sequencing of the libraries was done on the Illumina HiSeq 4000 platform by BGI Co. ...
-
bioRxiv - Bioengineering 2019Quote: ... The following primary antibodies were used for immunocytochemistry experiments: rabbit anti-glial fibrillary acidic protein (GFAP) (DAKO, 1:100, Z-0334, Lot #2002332), mouse anti-vascular endothelial (VE)-cadherin (Abcam ...
-
bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako, Santa Clara, CA, USA Z0311, 1:400). Following washing ...
-
bioRxiv - Neuroscience 2020Quote: ... Fragment size was determined by loading 1 ng/μl cleaned up PCR product on the Fragment Analyzer System (Agilent Technologies, Santa Clara, USA) using the qualitative DNA Kit dsDNA Reagent 35-5000bp (Cat No ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation with 50 µl/well of HRP-conjugated rabbit anti-mouse total IgG (1:1,000 in ELISA dilution buffer; P0260, Dako Agilent, Santa Clara, CA, USA), goat-anti-mouse IgG1 (1:8,000 in ELISA dilution buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... The recombinant transfer vector was linearized by PmeI and transformed into electro-competent E.coli strain BJ5183-AD-1 (Stratagene, Cat. No. 200157-11) for in vivo recombination with pAdEasy vector ...
-
bioRxiv - Biochemistry 2022Quote: ... with HistoVT One (L6F9587, Nacalai Tesque) and stained with the following primary antibodies: anti-insulin antibody (A0564, 1:400; Dako, Santa Clara, CA), anti-glucagon antibody (ab92517 ...
-
bioRxiv - Neuroscience 2020Quote: ... by co-transfecting pAAV-GFP or pAAV-GFP-Zdhhc14sh#1 with pAAV2 (pACG2)-RC triple mutant (Y444, 500, 730F) (Petrs-Silva et al., 2016) and pHelper (Stratagene, La Jolla, California) plasmids ...
-
bioRxiv - Plant Biology 2021Quote: ... RNA quality was assessed on 1% agarose gels and with the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). For each sample ...
-
bioRxiv - Plant Biology 2021Quote: ... The genomic SPL7 (AT5G18830.1) coding region (translational start to stop codon) was PCR-amplified and cloned into the pBluescript SK+ vector (Stratagene/Agilent Technologies, Waldbronn, Germany) which was used a PCR template for site-directed mutagenesis (A279T ...
-
bioRxiv - Cell Biology 2022Quote: ... Medium was changed 1-hour before the start of readout into 180 μL serum-free assay medium (Agilent Seahorse XF DMEM; 103575-100) supplemented with 4mM L-glutamine (Corning ...
-
bioRxiv - Pathology 2020Quote: ... Cambridge, MA), CD68 (LV n=25, IVS n= 26, RV n=22) for macrophages (1:800, clone KP1, Dako/Agilent, Santa Clara, CA), and CD163 (LV n=26 ...
-
bioRxiv - Pathology 2020Quote: ... followed by repeating all steps from antigen retrieval through TSA using an antibody against CK7 (1:200, mouse; Dako cat. no. MS-1352) and the FITC fluorophore (Perkin Elmer cat ...
-
bioRxiv - Plant Biology 2019Quote: ... and gene-specific primers (Supplementary Table 1) to a final volume of 20 μl in clear 96-well plates with clear plastic lids (Agilent Technologies, Waldbronn, Germany). QRT-PCR assays were performed using an AriaMx Real-Time PCR system (G8830A instrument ...
-
bioRxiv - Physiology 2021Quote: ... Antibodies bound to PCPE-1 were then detected by incubation with a HRP-conjugated rabbit anti-goat antibody (P0449; Dako; 50 ng/ml). After washing ...
-
bioRxiv - Systems Biology 2019Quote: ... fluorescent chemistry and 1 ng was used to obtain RNA Integrity Number (RIN) using the Bioanalyzer RNA 6000 Pico kit (Agilent Technologies, 5067-1513). Lowest RIN was 9.5 ...
-
bioRxiv - Microbiology 2019Quote: ... The reaction products for the total RNA of the human cell line KMM-1 were analysed by microfluidics-based automated electrophoresis with the Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2020Quote: The pooled library was diluted to ~1 ng/uL and run on a Bioanalyzer High Sensitivity DNA chip (Agilent, Santa Clara, CA, USA) to check library purity and to estimate the expected ratio of host to microbial amplicons in the sample.
-
bioRxiv - Immunology 2020Quote: The OCR and ECAR of THP-1 macrophages were determined using the Seahorse XFe24 Extracellular Flux Analyzer (Agilent Technologies, Santa Clara, CA, USA). Cells were seeded at 8 × 104 per well ...