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Citations for Agilent :
4101 - 4150 of 4652 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... using a C8 reverse phase micro-column (Zorbax 300SB-C8, 5μm, 5 × 0.3 mm, Agilent Technologies). The sample was then eluted with 70% of mobile phase B (flow rate of 50 μl/ min ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were visualized using a Cytation 5 microscope and Gen5 imaging software (Agilent, Santa Clara, CA).
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bioRxiv - Genomics 2019Quote: ... washed for 5 min in PBS 0.2% Triton-X100 and mounted using fluorescence mounting medium (DAKO). Stained slides were analyzed using a confocal laser scanning microscope (Leica CTR6500) ...
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bioRxiv - Immunology 2021Quote: ... - Kinetex EVO (5 μm, 100 Å)) on a HPLC system (Agilent, LC 1260 Infinity II, Agilent). A two-step gradient was applied ...
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bioRxiv - Microbiology 2020Quote: 5 μl of each sample was injected into XCT6330 LC/MSD ultra trap system (Agilent Technologies) equipped with a Nucleosil 100 C18 column (3 μm x 100 mm × 2 mm internal diameter ...
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bioRxiv - Cell Biology 2021Quote: ... Support fibers were generated from 5 wt% solutions of polystyrene (MW: 15,000,000 g/mol, Agilent Technologies) dissolved in xylene ...
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bioRxiv - Plant Biology 2022Quote: ... on a J&W DB-5 ms column (30 m × 0.25 mm × 0.25 μm, Agilent Technologies) and analyzed using a transmission quadrupole mass spectrometer (Agilent 5975 C series ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were consecutively incubated with: rabbit anti-total tau antibody (K9JA, Dako #A0024, 5 μg/ml), anti-rabbit secondary antibody conjugated with biotin (Invitrogen #A16114 ...
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bioRxiv - Microbiology 2023Quote: ... cells were grown with agitation at 37°C in an imaging multimode reader (Cytation 5, Agilent). Cell growth was monitored every 5 min by measuring absorbance at 600 nm ...
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bioRxiv - Zoology 2024Quote: ... We used an HP-5 column (30 x 0.25 mm ID X 0.25 μm, Agilent, US) with nitrogen (2 ml/min ...
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bioRxiv - Neuroscience 2024Quote: ... mut-shM4 (Table 5) and the QuikChange Multi Site-Directed Mutagenesis kit (200514; Agilent; Manufacturer Instructions) in several assays until all the mutations were incorporated ...
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bioRxiv - Bioengineering 2024Quote: ... we employed a bright-field imaging using a Cytation 5 cell imaging multimode reader (Agilent Technologies). Images were stitched and z-projected prior to analysis of MFI and cancer cell area ...
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bioRxiv - Microbiology 2023Quote: ... Samples were visualized using a Cytation C10 Confocal Imaging Reader and Gen 5+ software (Agilent BioTek).
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bioRxiv - Cancer Biology 2023Quote: ... Signal was developed with the Envision+ System/HRP Kit in 5–20 min (K4007, Agilent/Dako).
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bioRxiv - Cancer Biology 2023Quote: ... Signal was developed with the Envision+ System/HRP Kit in 5–20 min (K4007, Agilent/Dako).
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bioRxiv - Cell Biology 2022Quote: ... A 5 uL aliquot of the RNA was used for fragment analysis (AATI Fragment Analyzer, Agilent) to determine RNA size distribution ...
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bioRxiv - Microbiology 2023Quote: ... with agitation at 37°C for 16 hours in an imaging multimode reader (Cytation 5, Agilent). Optical density at 600 nm (OD600) ...
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bioRxiv - Cell Biology 2023Quote: ... with a custom Zorbax Eclipse XDB-C18 PrepHT column (Agilent, 21.2 x 250 mm, 5 μm) with a linear gradient of 5-50% CH3CN with 0.1% formic acid over 70-85 min with a flow rate of 6 mL min-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were blocked with 5 μg/ml human immunoglobulins solved in blocking serum-free medium (Dako) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of FAM104A isoform 5 was mutated using the QuikChange XL II kit (Agilent) with primers FAM104A_NL-RR_fwd (5’-cct cta ctt cca cat ccg cca gac ccg cag gga ggc cca ctt cc) ...
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bioRxiv - Microbiology 2023Quote: ... with GenePix Pro 6.1 (AutoPMT; pixel size of 5 µm) and analysed with GeneSpring 14.8 (Agilent).
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were run on a HP-5 Ultra Inert column (Agilent, 30m x 0.25mm, 0.25 μm). For analysis ...
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bioRxiv - Synthetic Biology 2024Quote: ... Chromatographic separation was carried out on a preparative C18 (5 µm, 150 x 21.2 mm; Agilent) column ...
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bioRxiv - Microbiology 2024Quote: ... The plate was then placed in a BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent) and incubated at 37 °C for 3 hours ...
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bioRxiv - Plant Biology 2019Quote: ... RNA quality and integrity were evaluated by electrophoresis on 1% agarose gel and the RNA integrity number (RIN) was analysed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.).
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bioRxiv - Microbiology 2019Quote: ... We extracted extracellular metabolites using solid phase extraction (SPE) with 1 g/6 cc PPL cartridges (BondElut, Agilent, Santa Clara, CA, USA) as described previously (Dittmar et al ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was diluted (1:10-20) and used for qRT-PCR in technical triplicate using Brilliant III SYBR master mix (Agilent Technologies, 600882) and CFX384 Touch Real-Time PCR Detection Systems (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4 °C followed by a 1 h incubation with either an HRP-conjugated goat anti-mouse IgG or goat anti-rabbit IgG (Dako, Cambridge, UK). Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Physiology 2019Quote: ... At least 10 sections per pancreas spaced at least 100 μm apart were stained with the following antibodies: Guinea pig anti-insulin (Dako, 1:500), Rabbit anti-proglucagon (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Five mL of headspace gas sample from the 20 mL culture bottle was administered via a 1 mL GSV Loop to the GC-MS (Agilent 7890A/5975C). The volatile compounds were separated by CP7348 column (Agilent PoraBOND Q 25 m × 250 µm × 3 µm ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μl of each PCR product was subjected to Chip-based capillary electrophoresis with the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA) and the proportional amount of each fragment to the whole yield of splicing products was calculated ...
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bioRxiv - Bioengineering 2021Quote: ... pCS2-GemininΔ27-GFP was generated by deleting amino acids 1-27 from Geminin-GFP [27] using QuikChange® Lightning mutagenesis kit (Agilent, 210513). The plasmids pCS2-Flag-FA-Geminin-GFP w.t and Δ27 variant were generated by cloning Geminin-GFP into pCS2-Flag-FA vector sing FseI and AscI restriction sites ...
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bioRxiv - Bioengineering 2021Quote: ... mRNA was isolated from high quality total RNA samples with RIN > 9 and 28S:18S > 1 (measured with an Agilent 2100 Bioanalyzer) using poly-T oligonucleotide beads ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a 2uL aliquot from each plated sample was diluted 1:9 on a Bravo liquid handling platform (Agilent, Santa Clara, CA, USA). A 5 μL aliquot from each diluted sample was arrayed into a 384 well plate for input into the mNGS library prep ...
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bioRxiv - Plant Biology 2021Quote: ... at 37⁰C (1:50 protein/trypsin ratio) and cleaned up with a C18 column (OMIX C18, Agilent, Santa Clara, CA, USA). Nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were loaded onto a ZORBAX SB-C18 column (150 x 1 mm, 3.5 μm, flow of 90 μl/min; Agilent, Les Ulis, France) heated at 40°C ...
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bioRxiv - Neuroscience 2021Quote: ... A capillary column was the stationary phase (30 m x 0.53 mm x 1 µm film thickness; Agilent Technologies, Santa Clara, CA), and helium was used as the mobile phase (at a flow rate of 8.5 mL/min) ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody detection was followed by 45 min of incubation with goat anti-mouse-biotin (DAKO, E0433, 1:200 in TNB blocking buffer) and subsequent incubation with Streptavidin-HRP Conjugate (PerkinElmer ...
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bioRxiv - Neuroscience 2019Quote: ... Endogenous peroxides were quenched with 1 % hydrogen peroxide in 10 % methanol for 10 minutes followed by blocking in antibody diluent (Dako /S 202230) for 10 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... and was allowed to pass through the packed catalyst bed at space velocities in the range of 1-45 s−1 by helium as the diluent gas.44 The injection was immediately followed by separation using HP-PLOTQ column (Agilent, 19091P-QO4), and compounds in the effluent stream were identified using online Agilent 5975C Triple-Axis MS detector ...
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bioRxiv - Microbiology 2020Quote: ... Cells were next washed three times with PBS and incubated with a secondary fluorescein isothiocynate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200) in 1% BSA for 1 h at 37°C ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies are diluted in 1x blocking solution and incubated with antibodies against insulin (1:2000, guineapig, Dako, cat. no. A-0546), glucagon (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... 190 to 601 nm (steps: 1 nm) and a Q-TOF (tandem mass spectrometry, quadrupole and Time of flight detector combined) (Agilent, Waldbronn, Germany). Electrospray ionization was performed in positive mode (ESI+ ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Immunology 2021Quote: ... and a 1:10 dilution of the resulting cDNA was run on a Bioanalyzer High Sensitivity DNA chip (Agilent Technologies #5067-4626) to assess cDNA quality and yield ...
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bioRxiv - Immunology 2021Quote: ... post-amplification and a 1:10 dilution of the resulting HTO libraries were run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess HTO quality and yield ...
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bioRxiv - Cell Biology 2021Quote: ... and deparaffinized prior to antigen retrieval in 0.1M sodium citrate pH6 and staining with guinea pig anti-insulin antibody (Dako A0564, 1:250) overnight at 4 degrees in the dark and visualized with Anti-Guinea Pig biotin conjugated antibody (Vectastain BA7000 ...
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bioRxiv - Biochemistry 2022Quote: ... The phPAI-1 library was generated by error prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) with primers that maintain the AscI and NotI restriction sites (SI Table 1) ...