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Citations for Agilent :
3651 - 3700 of 3719 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) was performed using a C3 column (Agilent Poroshell 300SB-C3, 1 x 75 mm, 5 µm) with a gradient of 5% to 95% acetonitrile (0.1% v/v formic acid ...
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bioRxiv - Cell Biology 2024Quote: ... the size distribution of each ChIP DNA sample was assessed by running a 1 µL aliquot on Agilent High Sensitivity DNA chip using an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The concentration of each DNA sample was determined using a high sensitivity Quant-iT™ dsDNA Assay Kit and a Qubit Fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-µm sections were cut and antigen retrieval was carried out by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies) for Ki67 ...
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bioRxiv - Molecular Biology 2021Quote: ... dilution 1:100)] in TNB blocking buffer for overnight at 4°C followed by one-hour incubation with secondary antibodies: EnVisionTM+ System-HRP (DAKO K4002). TRITC-based Tyramide reagent pack from Perkin Elmer was used to amplify the fluorescence of Ki67 and c-Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Sample pH was then adjusted to ~4 with formic acid prior to loading on Bond Elut-PH SPE columns (Agilent, 14102005), washing with aqueous buffer (30 mM ascorbic acid ...
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bioRxiv - Molecular Biology 2021Quote: ... The sections were incubated with a primary antibody at 4°C overnight and with a rabbit/mouse HRP-conjugated secondary antibody (Dako, Denmark) at room temperature for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated overnight at +4 °C with primary antibodies diluted in the same solution or in an antibody diluent (Agilent #S3022). The samples were then washed in 0.1 % Triton X-100 or 1.0 % Tween20 in PBS for 30-60 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... SDM was carried out with appropriate primers (Table 4) using QuikChange® Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) according to manufacturer’s instruction.
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bioRxiv - Cell Biology 2021Quote: Primary SVF cells from BAT and sWAT were isolated and cultured for 4 days before being plated in XF cell culture microplates (Seahorse Bioscience). SVF cells (10,000 cells ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed with 4 μl Brilliant III Ultra-Fast SYBR QPCR as instructed by the manufacturer (Agilent Santa Clara). All primers for quantification of either expression of viral m/cRNAs or antiviral responses are shown below.
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bioRxiv - Genetics 2022Quote: ... and incubated in the dark overnight at 4 C before running on a Novocyte Quanteon flow cytometer using NovoExpress 1.3.0 software (Agilent, Santa Clara, CA) at a flow rate of 14 uL/minute ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated overnight at 4°C and appropriate HRP-conjugated secondary antibodies (DAKO, Agilent Technologies, Santa Clara, CA, USA) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... or PDK1SKD-PIF S241A (4 mg/ml) was injected onto a Superdex 200 10/300 column (Cytiva) operated by a 1260 Infinity HPLC (Agilent Technologies). Light scattering of a 690 nm laser was detected by a MiniDawn Treos (Wyatt ...
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bioRxiv - Developmental Biology 2021Quote: ... for 6 minutes (30 seconds ON, 30 seconds OFF) at 4°C to obtain ~200 bp fragments (confirmed using an Agilent Bioanalyzer). Sonicated DNA was extracted using ethanol precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... All hESC lines were analysed for their genetic content by array-based comparative genomic hybridization (Human Genome CGH Microarray 4×44K, Agilent Technologies) as previously described (Jacobs et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in blocking solution (DAKO, Mississauga, Ontario, Canada). Sections were incubated overnight in polyclonal rabbit anti-GFAP primary antibody and monoclonal mouse anti-tau antibody (DC190) ...
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bioRxiv - Cancer Biology 2022Quote: Genome-wide copy number variations were assessed using the SurePrint G3 Human aCGH 4×180K Microarray (Agilent Technologies, Santa Clara, CA), according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... sections were post-fixed with 4% formaldehyde for 10 minutes and histologically stained with Dako Mayers hematoxylin (Agilent, cat. no. S3309) (4 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Bevacizumab-sensitive/resistant U87 cells were cultured identically to wild-type U87 cells.79 Cells were screened for mycoplasma every 3 – 4 months with the MycoSensor qPCR Assay Kit (Agilent Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... Sample pH was then adjusted to 4 with 15 μL of 20% formic acid before loading onto conditioned SPE Bond-Elute pH columns (Agilent, 14102062) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... solution (pH 7.4) and incubated with goat anti-mouse or anti-rabbit immunoglobulin labeled with dextran molecules and horseradish peroxidase (EnVision, Dako Omnis) at room temperature for 30 min ...
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bioRxiv - Genomics 2023Quote: Each purified GST-tagged DBD stock was validated for DNA-binding specificity using 4×44k universal protein-binding microarrays (PBMs) (Agilent Technologies), as described previously42 ...
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bioRxiv - Bioengineering 2023Quote: ... equipped with a UV detector through a C18 column Poroshell 120 EC-C18 (4.6 × 150 mm, 4 μm) (Agilent Technologies, Inc.). Mobile phase consisted of acetonitrile/methanol/0.4% acetic acid/ tetrahydrofuran (80:12:5:3 ...
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bioRxiv - Cancer Biology 2023Quote: The 4-6um thick TMA sections were deparaffinized and antigens were unmasked using EnVision FLEX Target Retrival Solution Low pH (Dako Agilent) in PT link device (PT200 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in a blocking solution (DAKO, Ontario, Canada). Sections were incubated overnight in a polyclonal rabbit anti-IBA-1 primary antibody (1:1000 ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Neuroscience 2024Quote: ... then stained with DAPI for 10 minutes at room temperature followed by 4 x 5-minutes washes in 1X PBS before being mounted using antifade fluorescence mounting media (Dako, S3023).
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bioRxiv - Cell Biology 2024Quote: Cy3-labeled cRNAs were further hybridized onto Agilent Whole Human Genome kit 4 x 44K slides (Catalog number G4112F, Agilent Technologies) containing 44,397 oligonucleotide probes in 2X Hi-RPM Hybridization buffer (Catalog number 5188-5242 ...
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and the OD600 was recorded every hour for 24 hours using a stacker (Biostack 4, Agilent BioTek) and plate reader (Epoch 2 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in primary antibodies overnight at 4 °C (Figure S2) and in secondary antibody-horseradish peroxidase complex (REAL EnVision detection kit, Dako #K5007) for 1 h at RT ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the stock solution was stored anaerobically at 4°C after its concentration was verified using inductively coupled plasma mass spectrometry (ICP-MS; Agilent 7800). Negligible oxidation of arsenite to arsenate was confirmed prior to use by high-performance liquid chromatography (HPLC ...
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and incubated at 37 °C for 24 h in a stacker-incubator system (Biostack 4, Agilent BioTek) connected to a plate reader (Epoch 2 ...
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bioRxiv - Genetics 2021Quote: ... split flow of 30 mL · min-1) into a gas chromatograph equipped with an FID detector and headspace autosampler (Agilent 7890 series; Palo Alto, CA, USA). Analytes were separated on a HP-5 capillary column (50 m by 320 μm by 1.05 μm column ...
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bioRxiv - Microbiology 2021Quote: ... split flow of 30 mL min-1) into a gas chromatograph equipped with a FID detector and headspace autosampler (Agilent 7890 Series; Palo Alto, CA, USA). Analytes were separated on a HP-5 capillary column (50m × 320 µm × 1.05 µm column ...
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Efficient breeding of industrial brewing yeast strains using CRISPR/Cas9-aided mating-type switchingbioRxiv - Microbiology 2021Quote: ... split flow of 30 mL min−1) into a gas chromatograph equipped with an FID detector and headspace autosampler (Agilent 7890 Series; Palo Alto, CA, USA). Analytes were separated on a HP-5 capillary column (50 m × 320 μm × 1.05 μm column ...
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bioRxiv - Neuroscience 2021Quote: Samples were removed from the −80°C freezer and allowed to warm to room temperature then vortexed for approximately 1 minute before being placed into the autosampler and held at 24°C (Agilent 1100 series autosampler, Palo Alto, CA) for the LC/MS/MS analysis ...
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bioRxiv - Microbiology 2022Quote: ... derivatization followed by gas chromatography – electron-ionization-time-of-flight mass spectrometry (GC-EI-TOFMS) in split mode (split 1:50, Agilent straight split liners with glass wool) was used15 ...
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bioRxiv - Cell Biology 2022Quote: ... pH 7.6) and then incubated with the secondary antibody (goat anti-mouse IgG antibodies coupled to horseradish peroxidase from DAKO at 1:5000 in Roti®-Block) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2% Milk powder and 0.5% Tween-20) and incubated with HRP-conjugated secondary antibody (diluted 1:2000 in Blocking buffer, rabbit α-mouse DAKO #0260, swine α-rabbit DAKO # P0217) for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... needles and green stem) of 4 individual clonal Pinus radiata trees using the Plant RNA Isolation Mini Kit (Agilent, Santa Clara, CA). 50 mg of plant material from each tissue type were used for the RNA extractions ...
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bioRxiv - Neuroscience 2020Quote: ... age range 4-14 years) were acquired using a 7T magnet with an Agilent DirectDrive console (Agilent Technologies, Santa Clara, CA, USA). A 2D diffusion-weighted spin-echo protocol was implemented with a single line readout (DW-SEMS ...
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bioRxiv - Neuroscience 2020Quote: ... blocked using 10% Normal Goat Serum (NGS) and incubated overnight with the following primary antibodies in 5% NGS overnight at 4°C: rabbit anti-GFAP (DAKO; #Z0334;1:1000), rat anti-L1-CAM (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... for 1 hour at room temperature or overnight at 4°C using the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) technique according to the manufacturer’s protocol (Dako, Carpinteria, CA, USA). All antibodies were diluted in TBS with 0.1% BSA ...
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bioRxiv - Microbiology 2020Quote: ... p-nitrophenyl-β-D-xylopyranoside and p-nitrophenyl-α-D-xylopyranoside were carried out by estimating the release of 4-nitrophenol (pNP) at 405 nm using a UV-Vis spectrophotometer (Agilent, G 68608) as described elsewhere (11).
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bioRxiv - Genetics 2023Quote: ... Kallisto quant settings were adjusted to -b 5 -l 160 -s 20 - -single - -threads 4 based on fragment lengths determined by the Agilent 4200 TapeStation (Agilent Technologies, Inc.)
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bioRxiv - Cell Biology 2024Quote: ... and HP-0921 from Agilent (m/z 922.0098) in positive mode and 4-NBA (m/z 166.0146) and HP-0921 from Agilent (m/z 966.0007, formate adduct) in negative mode.
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bioRxiv - Systems Biology 2024Quote: ... The sequencing libraries were quantified using the Qubit 4 fluorometer and library sizes were quantified using the D1000 ScreenTape assay (Agilent 5067-5582) on the TapeStation 4200 automated electrophoresis platform.
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bioRxiv - Biophysics 2024Quote: A 60,000 member tiling library of the Disprot database (release 2022 12) containing 16 residue-long peptides with 4-fold overlap was ordered from Agilent (G7270A, USA). The library was solubilised in 40 µL TE buffer yielding ∼1 µM concentration ...
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bioRxiv - Microbiology 2020Quote: ... For intact protein analysis,10 μL of desalted protein (∼1 mg/ml) was injected onto a Zorbax 300Å Stable Bond C8 column (Agilent Technologies, 4.6 x 50 mm, 3.5 μM) for reversed phase separation at 60°C and 0.4 mL/min ...