Labshake search
Citations for Agilent :
3601 - 3650 of 3719 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... All other synthetic or expressed peptide substrates were purified and analyzed at small scale utilizing analytical-scale reverse-phase HPLC on an Agilent 1100 series HPLC system (Santa Clara, CA) employing an Eclipse Plus C18 column (5 μm, 4 × 150 mm) (Agilent) at a flow rate of 1 mL/min ...
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bioRxiv - Immunology 2020Quote: Extracts and 4-OHE1 standard were analyzed using a liquid chromatography system (LC; 1200 series, Agilent Technologies, Santa Clara, CA) that was equipped with a reversed-phase analytical column (length ...
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bioRxiv - Cancer Biology 2022Quote: Cells were imaged using a 60x Plan Apo 1.4 NA objective on a Nikon Ti2-E microscope with a Yokogawa X1 spinning disk confocal system, MLC400B 4-line (405nm, 488nm, 561nm, and 647nm) dual-fiber laser combiner (Agilent), Prime 95B back-thinned sCMOS camera (Teledyne Photometrics) ...
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bioRxiv - Microbiology 2022Quote: ... One hundred μL of the extract was mixed with 10μL of 4-chlorobenzotrichloride as injection standard and analyzed by GC (HP 7890 Series GC, Agilent, USA) with a 20 m 0.18mm (18μm film thickness ...
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bioRxiv - Microbiology 2022Quote: ... 30 sec at 60°C (steps 3 and 4 were repeated 40 times) was done with the AriaMX real-time PCR System (Agilent).
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bioRxiv - Biochemistry 2024Quote: ... and 3D z-stacks were taken of each replicate well monitored every 4 hours for 48 hours using a Cytation C10 imager (Agilent) with confocal 546 nm excitation laser ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were immediately centrifuged at 2 000 x g for 20 min at 4 °C and left on ice until insertion into the Seahorse XFe96 Analyzer (Agilent) after the cartridge plate calibration cycle on the analyzer was complete ...
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bioRxiv - Cancer Biology 2023Quote: ... The adapter-modified DNA fragments were enriched by 4 cycles of PCR using SureSelect forward and SureSelect ILM Pre-Capture Indexing reverse (Agilent) primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated with Alamar Blue for 3-4 hours and then imaged with a Synergy Neo plate reader using excitation: 550 nm and emission: 590 nm (Agilent). The average plate background (media only with 0.1% DMSO ...
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bioRxiv - Microbiology 2023Quote: ... This system makes it possible to culture many bacterial strains in parallel in a replicated manner using many 96-well plates which are handled with a stacker (BioStack 4, Agilent Technologies ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Microbiology 2023Quote: ... Growth curves of OD578 were monitored every hour after 1 min of linear shaking under anaerobic conditions using an Epoch2 microplate reader coupled with a Biostack 4 microplate stacker (both Agilent) housed in a custom-made incubator (EMBL workshop24) ...
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bioRxiv - Biochemistry 2024Quote: ... mutations were inserted into the WT isolated (residues 193 – 261) RRM2 domain in a modified pGEX-6p1 vector (4) using the QuickChange method (Stratagene) and verified through Sanger Sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2024Quote: ... The quality and quantity of the enriched libraries were evaluated using Qubit® 4 Fluorometer and 4200 TapeStation System (Agilent). Sequencing was performed on an Illumina NovaSeq6000 machine with single-end 100 bp reads for NPM1 and H3K9me2 according to standard protocols.
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... and diluted to 3.5% nitric acid by adding 3800 μL milliQ water after which all test tubes containing samples were placed into an automatic sampler (SPS 4 Autosampler, Agilent) integrated with the MP-AES (model 4210 ...
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bioRxiv - Physiology 2024Quote: ... RNA concentration and integrity were evaluated by a fluorimetric assay (Qubit 4, ThermoScientific, USA) and capillary electrophoresis (TapeStation, Agilent, Germany), respectively ...
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bioRxiv - Plant Biology 2024Quote: ... Temperature-dependent phase transition was detected by measuring the optical scattering at 800 nm while increasing the temperature from 4 to 60°C (with a ramping rate of 0.5°C/min) using a spectrophotometer Cary 300UV-VIS (Agilent Technologies). Nitrogen gas purging was used to prevent the moisture from condensing on the cuvette surface ...
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bioRxiv - Physiology 2024Quote: ... sections were washed three times with cold 0.1% Triton in PBS and then incubated with primary antibodies at 4 °C overnight after being blocked with protein blocking solution (DAKO) for 5-10 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Nine images were taken every 4 hours in each well and the number of cells by images were determined using BioTek Gen5 Software (Agilent).
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bioRxiv - Immunology 2024Quote: 0.4 ug of biotinylated SL9 (SLYNTAVTL) or IV9 (ILKEPVHGV) peptides (NIH Tetramer Core Facility) were conjugated to PE-streptavidin (Agilent), APC-streptavidin (Agilent ...
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bioRxiv - Synthetic Biology 2024Quote: ... GFP fluorescence was measured every 15 min for up to 4 h and every 30 min thereafter (Mx3005P; Agilent Technologies). The slope of the GFP fluorescence from 0 to 4 h was evaluated for each aaRS activity ...
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bioRxiv - Synthetic Biology 2024Quote: ... GFP fluorescence was measured every 15 min for up to 4 h and every 30 min thereafter (Mx3005P; Agilent Technologies). The slope of the GFP fluorescence from 0 to 4 h was evaluated as the GFP synthesis rate ...
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bioRxiv - Bioengineering 2024Quote: ... we washed and cultured BMDMs in the Seahorse XF DMEM medium (pH 7.4) supplemented with 25 mM D-glucose and 4 mM Glutamine (all sourced from Agilent Technologies) for an hour in a non-CO2 incubator at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Images were automatically taken every 4 hours over 72 hours in a BioTek BioSpa Automated Incubator (Agilent, Santa Clara, CA). Cell proliferation was quantified using Aligent’s Gen5 image analysis tools.
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bioRxiv - Cancer Biology 2021Quote: ... MCF10A shCtrl and shEIF3E cell lines were transfected in 100 mm tissue culture plates with 5 μg of DNA at a ratio of 1:50 of a T7-tagged PRP4K expression vector (16) or pBluescript SK-(Stratagene/Agilent Technologies, Santa Clara, CA, USA) using the Neon Transfection System (Thermo ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2 or with negative isotype control mouse IgG2a (cat. X0943 - Agilent Technologies, Santa Clara, CA, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA integrity and quality were assessed by means of a 1% agarose electrophoresis gel and in Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Bioengineering 2020Quote: ... a high-power resistor of 1 Ω was placed in series with the coils and voltage drop across the resistor was observed with an oscilloscope (Agilent Technologies, Santa Clara, CA) during PEMF exposure period ...
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bioRxiv - Genomics 2020Quote: ... and assessed for quality using a 1 % (w/v) pulse-field gel electrophoresis and with genomic ScreenTape on the TapeStation 2200 platform (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... A 1 µL sample of the derivatized material was analyzed in splitless mode using a 7200 GC-QTOF system (Agilent Technologies, Santa Clara, CA, USA). A solution of fatty acid methyl esters (C8 to C30 ...
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bioRxiv - Cell Biology 2020Quote: ... and pom1521-200-GFP (pKBB520) fusions were generated by amplifying sGFP from pJK19-1 using PCR with PfuUltra™ HF (Agilent, Inc. Santa Clara, CA) and primers that generated chimeric Pom152-sGFP PCR products for homologous recombination in yeast ...
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bioRxiv - Microbiology 2021Quote: ... The integrity of RNA extracts was evaluated using gel electrophoresis on 1% agarose in 1x TBE and the Agilent 2200 TapeStation System (Agilent Technologies, Santa Clara, CA, USA). Libraries were prepared from three samples per treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein samples were separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblot analysis. Membranes were incubated with primary antibodies (Suppl. Table 1) overnight and probed with respective secondary antibodies (Dako-Agilent, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed with 1X phosphate-buffered saline (PBS) and incubated in a humidified chamber for 1 hour at room temperature with protein-blocking solution (Agilent Dako, Santa Clara, ON, Canada) to block nonspecific sites ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Plant Biology 2021Quote: ... Dried metabolites were methoximated (20 mg/mL methoxyamine in pyridine) and trimethylsilylated (MSTFA: TMSCI, 99:1) and then analyzed by GC-MS (Agilent 5975, GC/single quadrupole MS). GC-MS data were processed by Agilent MSD ChemStation ...
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bioRxiv - Microbiology 2020Quote: ... and D67N/K70R/T215F/K219Q viruses were generated by site-directed mutagenesis on an xxLAI HIV-1 backbone using the QuikChange XL Site-Directed Mutagenesis kit (Agilent Technologies Inc, Santa Clara, CA), according to the manufacturer’s protocols.
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bioRxiv - Microbiology 2023Quote: ... the cells were washed once with the same buffer and incubated with FITC-conjugated polyclonal swine anti-rabbit immunoglobulin antibody from DAKO (dilution 1:40 in BP) for 1 hr at 4°C on ice with shaking ...
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bioRxiv - Microbiology 2022Quote: ... CFSs were diluted in SDW (ratio 1/20) and injected into a high-performance liquid chromatography (HPLC) system (Agilent 1100 Series 203 HPLC System, Agilent Technologies, Palo Alto, CA, USA), equipped with a diode array and quaternary pump ...
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bioRxiv - Microbiology 2024Quote: ... Twenty-microliter reactions were prepared with 1×Brilliant III Ultra-Fast SYBR® Green Low ROX qPCR Master Mix (Agilent Technologies, Santa Clara, CA, USA), 1 µg μl-1 bovine serum albumin (New England Biolabs® Inc. ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Neuroscience 2023Quote: ... IDH 1 (R132H) (QM002; quartett GmbH, Potsdam, Germany), ATRX (BSB-108; Bio SB, Goleta, CA, USA) and Ki67 (MIB1; Agilent Technologies, Santa Clara, CA, USA) were routinely performed according to standard protocols.
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bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at room temperature for 1 hour and analyzed using gas chromatography-mass spectrometry (6890N GC, coupled to 5873N Mass Selective Detector; Agilent Technologies, Santa Clara, CA, USA). Statistical significance was assessed by mixed linear model with pBMI ...
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bioRxiv - Molecular Biology 2023Quote: Optimal temperature was measured using the enzyme activity assay described above in 1 mL cuvettes using a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Santa Clara, CA, USA) at 22 °C and temperatures from 30 °C to 50 °C with 5 °C increments using a circulating water bath ...
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VapC12 ribonuclease toxin modulates host immune response during Mycobacterium tuberculosis infectionbioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent, catalog no. DNF-474-1000). Genes with low expression value (mean FPKM value<0.5 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Immunodetection was carried out by blocking PVDF membranes in 5% (w/v) fat-free dried milk powder followed by incubation overnight in rat anti-FLAG antibodies diluted 1:500 (Agilent Technologies, Santa Clara, CA, USA). After a brief rinse in Tris-buffered saline with Tween™ 20 detergent (TBST) ...
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bioRxiv - Microbiology 2023Quote: K65R and M184V mutations in RT were generated by site-directed mutagenesis on an HXB2 or LAI HIV-1 backbone using the QuikChange XL site-directed mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... Desorption was performed at 250°C for 1’ (splitless mode) in the injection port of a 6890 N gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) coupled to PEGASUS 4D mass spectrometer (LECO Corporation) ...
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bioRxiv - Cancer Biology 2023Quote: ... IHC was performed on an automated Ventana Discovery Ultra staining system using primary antibodies recognizing Ki-67 (M7240, DAKO, mouse monoclonal, clone MIB1, 1:50) for 30min ...
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bioRxiv - Microbiology 2023Quote: Headspace CO2 of fungal incubations (1 mL) were measured for CO2 concentration on a Hewlett Packard HP 5890 Series II GC (Agilent Technologies, Inc, Santa Clara, USA) coupled with a Thermal conductivity detector (TCD) ...