Labshake search
Citations for Agilent :
3601 - 3650 of 4099 citations for 7 Bromo 1 2 3 4 tetrahydronaphthalen 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... and 2 µl of the solution was separated on a gas chromatograph (GC 7890A; Agilent Technologies) equipped with a Phenomenex ZB-35 (30m × 0.25mm × 0.25µm ...
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bioRxiv - Neuroscience 2020Quote: ... transferred on glass slides and mounted for visualization with anti-fading mounting medium (Agilent #S302380-2). Confocal images were acquired using a Nikon Eclipse C1si laser-scanning microscope equipped with a 20x (NA 0.75 ...
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bioRxiv - Biochemistry 2021Quote: ... in 2 mL headspace vials and analysed on an HPLC instrument (Agilent Technologies, 1260 Infinity II); peaks were identified using pure standards ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl of the ligation product was transformed into bacteria using XL10-Gold Ultracompetent Cells (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The pEGFP-C2-Myo15-2(jd) plasmid was generated using site directed mutagenesis (QuikChange II, Agilent) to introduce the jordan (c.4940A>G ...
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bioRxiv - Immunology 2021Quote: 2 × 105 BMDMs were plated into each well of Seahorse XF96 cell culture microplates (Agilent Technologies) and cultured overnight before treated with or without LPS plus ATP ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies against EGFR (2-18C9) and Ki-67 (M1B1) were obtained from Agilent (Santa Clara, CA). The anti-MCT1 antibody was obtained from Merck (Rockville ...
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bioRxiv - Genomics 2022Quote: ... Sections were incubated for 5min with hematoxylin (SigmaAldrich) followed by 2 min in bluing buffer (DAKO) and 10s in eosin Y (0.45M ...
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bioRxiv - Microbiology 2022Quote: ... Growth measurements were obtained in a 96-well plate using an Epoch 2 Microplate Spectrophotometer (Agilent) inside of an anaerobic chamber ...
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bioRxiv - Neuroscience 2022Quote: ... and were subsequently incubated with goat anti-rabbit labelled polymer EnVision+ Single Reagent (Dako, K400311-2) for 30 min at RT and peroxidase activity was revealed using DAB+ (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 10 minutes in H2O2 solution (S202386-2, Agilent Technologies, Santa Clara, CA, USA) to quench endogenous peroxidases ...
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bioRxiv - Cancer Biology 2024Quote: ... gaskets were removed and slides were mounted onto coverslips using Fluorescence Mounting Medium (S302380-2, Agilent). Images were acquired using a Zeiss Axio Scope.A1 fluorescence microscope with 40× and 100x magnifications and further analyzed with ImageJ ...
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bioRxiv - Genomics 2023Quote: ... then air dried and mounted using an aqueous fluorescence mounting medium (Agilent Dako, Cat# S302380-2). Cells were visualized and imaged using an Olympus UPLXAPO 20×/0.8 NA air objective on a spinning disk confocal microscope (SpinSR10 ...
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bioRxiv - Neuroscience 2023Quote: ... the labelled antibodies were developed with a Dako Liquid DAB+ substrate chromogen system (Dako; GV82511-2).
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bioRxiv - Bioengineering 2023Quote: ... Slides were then mounted with Dako fluorescence mounting media (S302380-2, Agilent Technologies, Santa Clara, US). The primary antibodies and respective concentrations used in this study are the following ...
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bioRxiv - Cell Biology 2023Quote: ... Optical density (OD) was measured with a BioTek Epoch 2 microplate spectrophotometer (Agilent, Santa Clara, CA) for at least 15 min to obtain blank values for each well at the target temperature ...
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bioRxiv - Neuroscience 2023Quote: ... we mounted the cultures on glass slides with DAKO anti-fading mounting medium (#S302380 − 2, Agilent) for confocal microscope imaging.
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bioRxiv - Bioengineering 2022Quote: ... the sections were buffered in DAKO wash buffer (Cat #K800721-2, Agilent, Santa Clara, CA, USA) before incubating in primary antibody diluted in DAKO antibody diluent (Cat# S080983-2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-CD45 clone 2B11 + PD7/26 (Ready-to-use, Agilent Dako cat. no. GA75161-2). After washing away the primary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-CD45 clone 2B11 + PD7/26 (Ready-to-use, Agilent Dako cat. no. GA75161-2). After washing away the primary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... The coverslips were then mounted on slides using DAKO Faramount Aqueous Mounting Medium (Agilent, #S302580-2). The slides were kept for 24h to allow the mounting media to cure before use.
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bioRxiv - Cancer Biology 2023Quote: FISH assay was performed on tissue microarrays using the Histology FISH accessory kit (K579911-2, Dako, Agilent Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... we mounted the cultures on glass slides with DAKO anti-fading mounting medium (#S302380 − 2, Agilent) for confocal microscope imaging.
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bioRxiv - Genomics 2023Quote: ... then air dried and mounted using an aqueous fluorescence mounting medium (Agilent Dako, Cat# S302380-2). Cells were visualized and imaged using an Olympus UPLXAPO 20×/0.8 NA air objective on a spinning disk confocal microscope (SpinSR10 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... slides were washed in blocking solution once more and treated with 3,3’-diaminobenzidine (DAKO K401011-2) for 5 minutes and counterstained with Gill 2 hematoxylin before brightfield imaging.
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bioRxiv - Plant Biology 2020Quote: ... The sample components were separated on a C18 olumn (Hypersil GOLD C18; 4.6 mm × 100 mm; 3 µm particle size; Agilent, Santa Cruz, CA, USA). The column was eluted at 0.3 mL/min with a gradient from 95% solvent A (0.1% formic acid in water ...
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bioRxiv - Developmental Biology 2022Quote: ... High quality total RNA (RNA integrity number = 10) was labelled with Cyanine 3 CTP using the Low Input Quick Amp Labelling Kit (Agilent Technologies-5190-2305), and purified using Qiagen’s RNeasy Mini Spin Columns ...
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bioRxiv - Microbiology 2021Quote: ... The extracts were acidified to pH 2 and additionally pre-treated with solid-phase extractions using Agilent Bond Elut-PPL 3 mL columns and diluted to 50 ppm (Agilent Technologies, DE, USA) following standard lab protocol29 ...
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- ACC TCC ACC ACC AGA ACC ACC TCC ACC GCT CTG AAA GTA CAG ATC CTC GGT ACC TTT ATA TAA TTC GTC CAT-3’) using the Agilent QuikChange SDM kit following the manufacturer’s instructions (Agilent, Singapore, Singapore; Cat: STR_210519). This was followed by amplifying the backbone of the plasmid using primer set Ag43BBGib_F ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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Age, sex, and cell type-resolved hypothalamic gene expression across the pubertal transition in micebioRxiv - Genomics 2023Quote: RNA-seq libraries were prepared using an automated - QuantSeq 3’mRNA-seq (Lexogen GmbH, Vienna) and Agilent NGS Workstation (Agilent Technologies, Santa Clara) at The Centre for Applied Genomics (TCAG ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF10A shCtrl and shEIF3E cell lines were transfected in 100 mm tissue culture plates with 5 μg of DNA at a ratio of 1:50 of a T7-tagged PRP4K expression vector (16) or pBluescript SK-(Stratagene/Agilent Technologies, Santa Clara, CA, USA) using the Neon Transfection System (Thermo ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2 or with negative isotype control mouse IgG2a (cat. X0943 - Agilent Technologies, Santa Clara, CA, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA integrity and quality were assessed by means of a 1% agarose electrophoresis gel and in Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Bioengineering 2020Quote: ... a high-power resistor of 1 Ω was placed in series with the coils and voltage drop across the resistor was observed with an oscilloscope (Agilent Technologies, Santa Clara, CA) during PEMF exposure period ...
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bioRxiv - Genomics 2020Quote: ... and assessed for quality using a 1 % (w/v) pulse-field gel electrophoresis and with genomic ScreenTape on the TapeStation 2200 platform (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... A 1 µL sample of the derivatized material was analyzed in splitless mode using a 7200 GC-QTOF system (Agilent Technologies, Santa Clara, CA, USA). A solution of fatty acid methyl esters (C8 to C30 ...
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bioRxiv - Cell Biology 2020Quote: ... and pom1521-200-GFP (pKBB520) fusions were generated by amplifying sGFP from pJK19-1 using PCR with PfuUltra™ HF (Agilent, Inc. Santa Clara, CA) and primers that generated chimeric Pom152-sGFP PCR products for homologous recombination in yeast ...
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bioRxiv - Microbiology 2021Quote: ... The integrity of RNA extracts was evaluated using gel electrophoresis on 1% agarose in 1x TBE and the Agilent 2200 TapeStation System (Agilent Technologies, Santa Clara, CA, USA). Libraries were prepared from three samples per treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein samples were separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblot analysis. Membranes were incubated with primary antibodies (Suppl. Table 1) overnight and probed with respective secondary antibodies (Dako-Agilent, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed with 1X phosphate-buffered saline (PBS) and incubated in a humidified chamber for 1 hour at room temperature with protein-blocking solution (Agilent Dako, Santa Clara, ON, Canada) to block nonspecific sites ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Plant Biology 2021Quote: ... Dried metabolites were methoximated (20 mg/mL methoxyamine in pyridine) and trimethylsilylated (MSTFA: TMSCI, 99:1) and then analyzed by GC-MS (Agilent 5975, GC/single quadrupole MS). GC-MS data were processed by Agilent MSD ChemStation ...
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bioRxiv - Microbiology 2020Quote: ... and D67N/K70R/T215F/K219Q viruses were generated by site-directed mutagenesis on an xxLAI HIV-1 backbone using the QuikChange XL Site-Directed Mutagenesis kit (Agilent Technologies Inc, Santa Clara, CA), according to the manufacturer’s protocols.
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bioRxiv - Microbiology 2023Quote: ... the cells were washed once with the same buffer and incubated with FITC-conjugated polyclonal swine anti-rabbit immunoglobulin antibody from DAKO (dilution 1:40 in BP) for 1 hr at 4°C on ice with shaking ...
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bioRxiv - Microbiology 2022Quote: ... CFSs were diluted in SDW (ratio 1/20) and injected into a high-performance liquid chromatography (HPLC) system (Agilent 1100 Series 203 HPLC System, Agilent Technologies, Palo Alto, CA, USA), equipped with a diode array and quaternary pump ...
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bioRxiv - Microbiology 2024Quote: ... Twenty-microliter reactions were prepared with 1×Brilliant III Ultra-Fast SYBR® Green Low ROX qPCR Master Mix (Agilent Technologies, Santa Clara, CA, USA), 1 µg μl-1 bovine serum albumin (New England Biolabs® Inc. ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Neuroscience 2023Quote: ... IDH 1 (R132H) (QM002; quartett GmbH, Potsdam, Germany), ATRX (BSB-108; Bio SB, Goleta, CA, USA) and Ki67 (MIB1; Agilent Technologies, Santa Clara, CA, USA) were routinely performed according to standard protocols.