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Citations for Agilent :
3551 - 3600 of 3899 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Patient-matched primary tumor tissues and PDTOs were immunostained for diagnostic NSCLC markers with antibodies against cytokeratin (CK)7 (mouse, M7018, 1:100; Dako), CK5/6 (mouse ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous peroxidases were blocked by incubation with 3 % H2O2 and then blocked for 1 h (Dako Serum-free protein block). Sections were then incubated with the 1st primary antibody (FABP7 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed in PBS and incubated in DAPI (1:500) for 5 min before mounting with the immunofluorescence mounting media (DAKO).
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bioRxiv - Plant Biology 2023Quote: ... RNA integrity was confirmed by agarose (1% w/v) gel electrophoresis and by microfluidic electrophoresis using a Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... For staining of macrophages and platelets the aortic tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting peptide mixture was acidified by addition of 1% trifluoroacetic acid (TFA) and desalted on a reversed phase (RP) C18 OMIX tip (Agilent). The tip was first washed 3 times with 200 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
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bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
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bioRxiv - Cancer Biology 2024Quote: ... and anti-GGCT (16257-1-AP; proteintech) antibodies were used for immunodetection on rehydrated sections pretreated with Target Retrieval Solution (S1699; DAKO). The Envision™+ system (HRP/DAB ...
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bioRxiv - Genomics 2022Quote: ... and 1 μl was removed to assess tagmentation fragment size and yield by D5000 ScreenTape (Catalog No. 5067-5588, Agilent) on an Agilent Tapestation.
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bioRxiv - Cell Biology 2022Quote: ... Cells were cultured for 2-days in complete RPMI to allow for attachment to the culture dish and then for 1-hour in XF RPMI medium (Agilent) with 2.8 mM glucose ...
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bioRxiv - Biochemistry 2022Quote: ... Astrocytes were pre-incubated with 10 μM CGP-37157 or DMSO for 1 h and then plates were inserted in a XFe24 Seahorse Analyzer apparatus (#102238-100, Agilent). Cells were stimulated with 100 μM ATP ...
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bioRxiv - Biophysics 2022Quote: TTP was run on a tandem size exclusion chromatography - multi-angle light scattering detector by injecting 100 μL sample at a concentration of 1 mg/mL using a 1260 Infinity HPLC (Agilent). The column was pre-equilibrated in 0.1 μm filtered buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were acidified by 1% trifluoroacetic acid (TFA) and C18 cleanup was performed using Bravo AssayMAP robot with C18 cartridges (Agilent) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively, 1:5000 for Western blot). The rabbit polyclonal anti-FBP17 (1:1000 for Western blot ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently rinsed with PBS supplemented with 0.01% Tween 20 and probed with horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (1 in 500 dilution) (DAKO, Agilent Technologies) for 40 min ...
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bioRxiv - Plant Biology 2022Quote: ... adsorption at 45°C for 5 minutes and desorption at 250°C for 1 minute onto a DB-624 capillary column with 60m x 0.25mm x 1.40μm film thickness (Agilent Technologies, Böblingen, Germany). The GC temperature was programmed as follows ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-GFAP immunohistochemistry (Polyclonal Rabbit Anti-Human, Proteinase K enzymatic antigen retrieval, dilution 1:1000, DAKO Omnis, Code Number: GA524) was employed to assess reactive astrogliosis ...
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bioRxiv - Molecular Biology 2023Quote: ... we used horseradish peroxidase (HRP)-conjugated isotype-specific anti-rabbit IgG or anti-mouse IgG (1:10000; DAKO, Cambridgeshire, UK) secondary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Bone marrow was flushed out using a syringe and 1 ml of PBS + 5% FBS and filtered through a 40 μm strainer (Dako). Cells were counted and analyzed immediately ...
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1 ERG (GenBank accession. NM_182918) Myc-tagged construct was mutated using the QuickChange Lightning Multi SiteDirected Mutagenesis kit (Agilent: #210513). All primers were designed using the QuickChange Primer Design Program (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with 1x PBST for 5 min and incubated with secondary antibodies anti-mouse-HRP (1:10,000) (Agilent Dako, #P0447) and anti-rabbit-HRP (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality was determined by running 1 μL of the sample on a BioAnalyzer RNA Nano chip (Agilent, No. 5067-1511) for RIN ...
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bioRxiv - Genomics 2023Quote: ... The percentage of the genome fragmented to sizes between 1 – 4 kb was then empirically measured by fragmenting 1 µg of genomic DNA followed by quantification on a Genomic DNA ScreenTape assay (Agilent). Hpy166II fragments 41% of the genome to within the target size range ...
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bioRxiv - Plant Biology 2023Quote: CDS sequences from PhDEF and PhGLO1 were amplified from Petunia x hybrida R27 inflorescence cDNAs with primers MLY2382/MLY2383 and MLY2384/2385 respectively (Supplementary Table 1) and cloned into the in vitro translation vector pSPUTK (Stratagene) by NcoI/XbaI restriction ...
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue samples were incubated for 60 min at room temperature with primary antibodies (Monoclonal Mouse Anti-Human Ki67, clone MIB-1, Dako North America Inc. ...
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bioRxiv - Systems Biology 2023Quote: ... we calibrated the TIMS elution voltages by known 1/K0 values from at least two out of three ions from Agilent ESI LC/MS tuning mix (m/z ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-incubated for 1 h and treated in Agilent Seahorse XF Base Medium (Agilent Technologies, cat#103334-100), which is optimized for extracellular flux assays and contains thiamine at 8.2 μM concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... after which live cell imaging analysis was carried out for 30 minutes at a rate of 1 frame per 30 or 40 seconds using BioTek LION Heart XL (Agilent) or TomoStudio (Tomocube ...
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bioRxiv - Cancer Biology 2024Quote: ... These tumor sections were stained at the Histology core (Henry Ford Health) according to the manufacturer’s protocols (Ki-67 IHC MIB-1, Dako Omnis). Proliferating cells were immunostained with FLEX monoclonal mouse anti-human Ki-67 (Catalog No ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: The following secondary antibodies were used in this study: rabbit anti-mouse immunoglobulins/HRP (Agilent Cat# P0260, RRID:AB_2636929, 1/10000), goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... The library profiles were checked using High Sensitivity NGS Fragment Analysis kit (1-6000bp) (Agilent Technologies, reference DNF-474-1000) on the 5300 Fragment Analyzer System ...
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bioRxiv - Cell Biology 2024Quote: ... Qualitative and quantitative measurement of cDNA was conducted with the High Sensitivity NGS Fragment 1-6000bp kit on a 48-channel Fragment Analyzer (Agilent) and 1x dsDNA kit on the Qubit 4 Fluorometer (ThermoFisher) ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue was hybridized with an antibody against CD138/Syndecan-1 (clone MI15, mouse, #M7228, Agilent, Santa Clara, CA, USA), following standard procedures by automated processing using the Tecan Evo Freedom stainer and the Dako Advance system according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: MBP-MPS1104-330/WT and MPS1104-330/4A were obtained by over-expression in 1 L culture of BL21CodonPlus(DE3)-RIL cells (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... infected with VSVΔM51-GFP at the indicated MOIs and treated with 0.3-3µM CBL0137 (or media alone) 1 hpi using the Agilent Bravo liquid handling system (Agilent, CA, USA). 24 hpi ...
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bioRxiv - Microbiology 2024Quote: ... The amplified vectors were digested with Dpn1 and 1 µL of each (vector and promoter region insert) were transformed into ultracompetent XL-10 Gold cells (Agilent) for in vivo assembly ...
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bioRxiv - Immunology 2024Quote: ... Serum samples were diluted 1:50 in PBS + 25% FBS and data were acquired with a BioTek Synergy H1 plate reader (Agilent). ELISA data were analyzed with GraphPad Prism (Dotmatics ...
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bioRxiv - Microbiology 2024Quote: ... membranes were immunoblotted for 1 hour with secondary antibodies (1:5,000) anti-mouse or anti-rabbit linked to peroxidase (reference number: P0260 and P0217, respectively, Dako Agilent). Signal for (3xFLAG)TamL was generated using Clarity™ Western ECL substrate chemiluminescence reagent (BioRad) ...
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bioRxiv - Neuroscience 2024Quote: ... The antigen retrieval process involved incubating the sections with DAKO Target Antigen Retrieval Solution (diluted 1:10; Agilent Technologies, USA) in a water bath at 90 °C for 45 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... One aliquot was solubilized with 1% SDS to determine protein content and the other aliquot was mixed with 1 mL 30% HNO3 and subjected to ICP/MS 8900 (Agilent). Data was normalized by the corresponding protein concentration.
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bioRxiv - Plant Biology 2024Quote: ... A splitless injection volume of 1 μL was injected onto an HP-5 column with 5% phenyl methylpolysiloxane stationary phase (Agilent) with dimensions of 30 m x 0.25 mm x 0.25 μm ...
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bioRxiv - Biochemistry 2024Quote: The HAdV5HVR7 vectors (“1st generation” vectors) were obtained by cloning the gene of interest into the pAdEasy-1 plasmid system from Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... 2 mM Seahorse XF L-glutamine solution and 1 mM Seahorse XF pyruvate solution (103577-100, 103579-100 and 103578-100, all from Agilent) and cells were kept in a CO2 free incubator for 45 min prior to starting the Cell Mito Stress Test ...
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bioRxiv - Immunology 2024Quote: ... Sections were blocked with 5% horse serum in 1% BSA solution and incubated with the anti-CD68 primary antibody (KP1, Dako) for 60 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... which was amplified in 15 PCR cycles using Herculase II Fusion DNA polymerase in a 100-μl reaction mixtures (1x Herculase II reaction buffer, 1 mM dNTPs, 200 pM Agilent library ...
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bioRxiv - Neuroscience 2024Quote: Cells were counted and plated at 1 × 105 (mouse astrocytes or neurons) cells per well in a Seahorse XF24 Cell Culture Microplate for all experiments (Agilent). Cells were then treated with indicated inhibitors or agonists in each experiment for 20 h ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then incubated in a 0% CO2 chamber at 37 °C for 1 h before being placed into a Seahorse XFe24 Analyzer (Agilent). For OCR and ECAR mitostress test experiments ...