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Citations for Agilent :
3401 - 3450 of 3899 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: The following secondary antibodies were used for western blot experiments: peroxidase-conjugated goat anti-rabbit (P448, DAKO, 1:1000) and goat anti-mouse (P0447 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and an Agilent 7693A autosampler fitted with a 100 μL gastight syringe was used to inject headspace gas (50 μL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 μm film; Agilent). Data analysis was performed using Agilent MassHunter WorkStation Quantitative Analysis ...
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bioRxiv - Synthetic Biology 2024Quote: ... and an Agilent 7693A autosampler fitted with a 100 μL gastight syringe was used to inject headspace gas (50 μL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 μm film; Agilent), and (iii ...
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bioRxiv - Physiology 2024Quote: ... live cells stained with Hoechst 33342 (1:2,000 dilution) (ThermoScientific, Cat#62249) were enumerated using Cytation 5 plate reader (BioTek Instruments - Agilent). Values obtained from the Mito-Stress test were normalized per 1,000 cells.
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bioRxiv - Genomics 2024Quote: ... The quality of nucleic acids was assessed by agarose gel electrophoresis (1% for RNA, 0.7% for DNA) and on-chip electrophoresis (Bioanalyzer 2100, Agilent).
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bioRxiv - Cell Biology 2024Quote: ... GC-FID was performed using an Agilent 7890 gas chromatograph equipped with a DB-WAX column (15 m x 0.53 mm, 1 µm; Agilent) and flame ionization detection ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using the 1-Step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies) and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Rehydrated 5-μm sections were stained with primary antibodies against insulin (dilution 1:1000; Dako, Santa Clara, CA, USA), somatostatin (dilution 1:300 ...
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Microbiology 2023Quote: ... RNA integrity was assessed with 1% (m/v) agarose gel and Agilent 2100 Bioanalyzer System (Agilent Technologies, 5067-4626). For antibiotic treatment conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... the beads were transferred into a 1-mL PP filtration microplate (Agilent, 7 µm frit, cat. no. 202501-100) and washed 3x with 200 µL 1x PBS and 2x 200 µL ultrapure water ...
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bioRxiv - Biophysics 2023Quote: Drp1 isoform 1 (Genbank accession number AB006965) was PCR amplified with Pfu Turbo DNA polymerase (Stratagene, La Jolla, CA) as an NdeI/XhoI fragment ...
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bioRxiv - Biochemistry 2023Quote: ... and m6A(1-100 mM, MCE HY-N0086) were analyzed on RapidFire 300 high-throughput SPE system (Agilent Technologies) interfaced with a Sciex 6500 (Sciex) ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary anti-mouse-HRP (Santacruz sc-516102 Lot#c1419) was diluted 1: 10,000 or anti-rabbit-HRP (Dako P0448) 1:1000 in blocking buffer and incubated for 1hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... RNA quality was measured by 1% agarose gel electrophoresis (Figure S3A) and by High Sensitivity (HS) DNA Assay (Agilent) (Figure S3B).
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Synthetic Biology 2023Quote: ... An Agilent 7693A autosampler fitted with a 100 µL gastight syringe was used to inject headspace gas (50 µL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 µm film; Agilent). Experiments were performed using a 55:1 split ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then rinsed with PBS containing 1% FBS prior to imaging using a Cytation 5 instrument (Agilent BioTek). Mean fluorescence was calculated using Gen5 software (RRID:SCR_017317).
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bioRxiv - Cell Biology 2024Quote: ... and qualitatively assessed with the High Sensitivity NGS Fragment 1-6000bp Kit on a 96-channel Fragment Analyzer (Agilent). Final libraries were normalized ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were also assessed for size distribution and adapter dimer presence (<0.5%) by the High Sensitivity NGS Fragment 1-6000bp kit on a 96-channel Fragment Analyzer (Agilent). All sequencing libraries were then normalized on the MicroLab STAR (Hamilton) ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added with the estimation that OD600 of 1 is approximately equal to 8×108 CFU/ml (Agilent biocalculator). The MOI of 0.01 was administered to E ...
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bioRxiv - Immunology 2024Quote: ... the slides were washed in 1× PBS and then exposed to Dako Serum-free Protein Block solution (Agilent, USA) for 45 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed in PBS-T (0.05% Tween- 20 in PBS) and PBS before incubation with either 1:20,000 dilution of HRP-conjugated rabbit anti-human IgG (Dako) or 1:10,0000 dilution of HRP-conjugated goat anti-mouse IgG (Seracare ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were probed with appropriate primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1/5000 (Dako Agilent). Proteins were detected with ECL Western Blotting Substrate (Promega ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were probed with appropriate primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1/5000 (Dako Agilent). Proteins were detected with ECL Western Blotting Substrate (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody solution contains mouse anti-smooth muscle actin (SMA) antibody (1:67, Dako, M0851, Santa Clara, CA) for labeling arteries ...
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bioRxiv - Microbiology 2024Quote: ... membranes were immunoblotted for 1 hour with secondary antibodies (1:5,000) anti-mouse or anti-rabbit linked to peroxidase (reference number: P0260 and P0217, respectively, Dako Agilent). Signal for (3xFLAG)TamL was generated using Clarity™ Western ECL substrate chemiluminescence reagent (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... We characterized a 1/10 dilution of both spikes using the TapeStation RNA HS kit (Agilent Technologies, CA, USA) and compared the true size distribution to the theoretical one (as calculated using the manufacturer’s molarity and sequence files).
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies used for detection were PHF1 (pSer396/404) tau (1:1000, Peter Davies, Cat# PHF1; RRID: AB_2315150) and anti-tau (Dako, 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and the chambers were left to dry before imaging with Axio Observer.Z1/7 (Zeiss) or the BioTek Cytation 1 (Agilent).
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibody was a mouse monoclonal anti-human beta-catenin antibody (Clone beta-catenin 1, M3539, Dako, Agilent) used at 1:100 dilution.
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibody was a mouse monoclonal anti-human beta-catenin antibody (Clone beta-catenin 1, M3539, Dako, Agilent) used at 1:100 dilution.
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bioRxiv - Bioengineering 2024Quote: ... The reaction was initiated by adding purified RnKICD wild-type or mutant enzyme (0.1 mg mL-1) to the reaction mixture into 20 mL GC-MS headspace vials (5188-2753, Agilent) sealed with PTFE-lined screw caps (5188-2759 ...
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bioRxiv - Biochemistry 2024Quote: ... The cHMM or cS1 cDNA was cloned into the pShuttle-IRES-hrGFP-1 vector (Agilent Tech., Santa Clara, CA) and an Ad-Myo-Flag virus was prepared and amplified for expression of cHMM/cS1 protein in C2C12 cells ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected into the GC (Sigma-Aldrich 5973 Network mass selective detector attached to an Agilent 6890 gas chromatograph (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... Two µl of samples were introduced in the splitless injection mode in DB-5 (60 m × 0.25 mm, 1 µm film thickness, Agilent, CA) using helium as a carrier gas ...
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bioRxiv - Cancer Biology 2021Quote: ... The plate was incubated in a CO2-free 37°C incubator for 1 hour and a Mitochondrial Stress Test (Agilent) was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... S34F or Q157R were treated with doxycycline (1 μg/mL) for 48 hours before UV-crosslinking (400 mJ/cm2 UV Stratalinker 2400, STRATAGENE). RNA-protein complexes were immunoprecipitated with 12 μg anti-FLAG M2 antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... the specimens were detected by incubating with goat anti-rabbit or anti-rabbit/mouse IgG-HRP for 1 h and subsequently with DAB (Dako). The tissue sections were then counterstained with 0.1% (wt/vol ...
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bioRxiv - Cell Biology 2020Quote: ... ∼100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... and the cDNA hybridised to an 8 Chamber Agilent slide at 65°C for 16 hours before washing the slide with Oligo aCGH Wash Buffer 1 (Agilent) for 5 minutes at room temperature and Oligo aCGH Wash Buffer 2 (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent) (m/z 622 ...
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bioRxiv - Molecular Biology 2021Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent) (m/z 622 ...
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bioRxiv - Developmental Biology 2020Quote: ... then 1 ml of the headspace gas was withdrew and injected into a gas chromatograph (Agilent GC 6890N, CA, USA) fitted with a flame ionization detector and an activated alumina column (Zhang et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Size of chromatin fragments was verified on a 1% agarose gel and on a high sensitivity DNA chip in a 2100 bioanalyzer (Agilent) after decrosslinking overnight at 65°C with shaking in elution buffer (100 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
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bioRxiv - Molecular Biology 2021Quote: Reverse transcription for PCR analysis was performed using 1 μg total RNA and 300 ng random hexamers using Affinity script multiple temperature reverse transcriptase (Agilent). For UTP11 ...
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bioRxiv - Immunology 2022Quote: ... for 1 hour RT and replacing the secondary antibody incubation with HRP-labeled polyclonal goat α-mouse antibody (Dako K4000) for 30 minutes RT ...
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bioRxiv - Microbiology 2020Quote: ... EnvP(b)1CS- with the furin cleavage site mutated from RKTR to SKTR was generated from the human EnvP(b)1 plasmid using QuikChange site-directed mutagenesis (Agilent). EnvP(b)1 iRBD sequences were synthesized and cloned into a modified pVRC8400 expression vector ...