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Citations for Agilent :
301 - 350 of 501 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: A premade human placenta plasmid cDNA library in the pCFB XR vector (ViraPort XR) was purchased from Stratagene. Retroviruses were produced in 293T cells followed by infection of target 293T cells according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’mitoUTR was cloned into the TFAM-mScarlet/pWPXL or the 3’mitoUTR_TFAM-mScarlet_pWPXL plasmid using megaprimers following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol provided by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... was used as a template for generating expression plasmids with FH variants using the QuikChange II kit (Agilent). Sanger sequencing was used to confirm sequence identity for all plasmids ...
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bioRxiv - Biophysics 2024Quote: The WT-CtNAT10-pFastBacHT A plasmid was used to prepare all CtNAT10 mutants using the QuikChange protocol (Stratagene) 53 ...
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bioRxiv - Molecular Biology 2024Quote: ... Expression plasmids were constructed with the obtained PCR product using the SureVector system (Agilent, Santa Clara, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... All GαS variants were obtained from this plasmid by site-directed mutagenesis using the QuikChange II kit (Agilent) and primers listed in Table S2 ...
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bioRxiv - Genomics 2024Quote: ... the pETM11-Sumo3-Tn5(E54K,L372P) plasmid was transformed into Escherichia coli BL21(DE3) codon + RIL cells (Stratagene). Cells were grown in TB-FB media (1.2 % Bacto-Tryptone ...
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bioRxiv - Genetics 2021Quote: ... we introduced the corresponding single nucleotide mutation in the 3xFLAG donor plasmid using QuikChange Site-Directed Mutagenesis Kit (Agilent). Then this donor plasmid was used for the same process used to generate the BAG3WT-FLAG line25 ...
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bioRxiv - Bioengineering 2022Quote: ... SARS-CoV-2 RBD mutant plasmids were generated by Quikchange site-directed mutagenesis according to manufacturer’s instructions (Agilent, 210513). Biotinylated proteins were made by co-transfecting Avitagged RBD plasmids with a BirA expression plasmid and into Expi293 cells using FectoPRO ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid point mutations were generated using Pfu Ultra polymerase by Quick Change PCR mutagenesis (Agilent Technologies, Santa Clara, CA). The resulting DNA constructs and strains were verified by DNA sequencing.
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bioRxiv - Microbiology 2020Quote: ... Unit genome-length fragments (approximately 2,600 nucleotides) were excised and ligated into the pBLUESCRIPT-KS+ (pKS+) plasmid vector (Stratagene), previously cleaved with the same enzyme ...
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bioRxiv - Cell Biology 2021Quote: ... A plasmid with smc3-K112,113R (AMp1392) was generated by site-directed mutagenesis (using QuikChange XL Site-directed mutagenesis kit, Agilent). Both plasmids were integrated into the LEU2 locus in the pCLB2-3HA-SMC3 strain background.
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bioRxiv - Immunology 2022Quote: ... Amber (TAG) codons were introduced site-specifically in the 2B4 TCR and CD3 plasmid using Quikchange mutagenesis kit (Agilent).
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bioRxiv - Microbiology 2022Quote: Single-base alterations to plasmid sequences were performed using a PCR technique based on QuikChange site-directed mutagenesis (Stratagene).
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bioRxiv - Biophysics 2022Quote: Site-directed mutagenesis of the human yb-1 coding gene was carried out directly on the pET22b-YB-1_1-180 expression plasmid by using the “Quikchange II XL site-directed mutagenesis kit” from Stratagene and appropriate oligonucleotides (Eurofins Genomics) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we deleted two existing Bsa-I sites from the plasmid by site-directed mutagenesis (Agilent, Santa Clara, CA, #200521). In the clonal strain production ...
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bioRxiv - Microbiology 2021Quote: ... Cassettes and the template plasmid pM07704 were amplified by polymerase chain reaction (PCR) with Herculase II DNA polymerase (Stratagene). Marker-exchange plasmids were generated by ligation of the PCR products in α-select cells (Bioline ...
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bioRxiv - Microbiology 2021Quote: ... Storz and pRprAmut2 was constructed by amplification of the pRprA plasmid with mutagenic primers using the Pfu enzyme (Agilent), followed by DpnI digestion and transformation into the cloning strain NEB5-alpha F’Iq ...
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bioRxiv - Microbiology 2019Quote: ... and pGal4-UAS-Luc plasmids (0.3 µg/well; provided by Dr. Yves Jacob) or IFN-β-pGL3 (0.3 µg/well; Stratagene) together with pRL-CMV reference plasmid (0.03 µg/well ...
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bioRxiv - Immunology 2019Quote: ... the parental Env-encoding plasmid was altered by site-directed mutagenesis using the QuikChange site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... The region containing random fragments of the VWF plasmid was made double stranded using primers P9/P10 (annealing temperature = 65.0°C) and Herculase II (Agilent) with 16-20 rounds of PCR amplification (Figure S1B) ...
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bioRxiv - Genomics 2021Quote: ... coli were generated by introducing the corresponding mutations in plasmid pDL893 using the QuikChange site-directed mutagenesis kit (Stratagene).
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bioRxiv - Biochemistry 2021Quote: Individual point mutations were introduced into AsCap5 plasmid by Quick-change mutagenesis according to the direction provided by Agilent. Oligonucleotides employed for creating mutants were ordered from IDT ...
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bioRxiv - Neuroscience 2021Quote: ... Fusion products were digested with NotI and ligated to a NotI cleaved pAAV-MCS plasmid backbone (#240071-5, Stratagene). The final rAAV/SORL1 KO-Neo plasmid construct was verified by NotI digestions and sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... Briefly, Flag-NLS-linker-Cas9 (Baker et al., 2016) was co-electroporated with a pAAV backbone-based plasmid (Stratagene) containing two U6-promotor-driven gRNAs targeting HPRT (GACTGTAAGTGAATTACTT) ...
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bioRxiv - Microbiology 2020Quote: ... Site-directed mutagenesis of plasmids was performed by DNA synthesis with 2.5 U PfuUltra II Fusion HS DNA Polymerase (Agilent), 0.2 μM oligonucleotide encoding the change ...
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bioRxiv - Microbiology 2022Quote: ... the vector pEC1_ptpA previously constructed [22] was used as template to generate the suicide plasmid pEC1_ptpA_D120A derivative by using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies) with the primer #1559 (5’-GGAAGAGAGTGATGTACCAGCTCCATACTACACGAATAATT-3’) ...
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bioRxiv - Cancer Biology 2022Quote: ... PLK1 mutant plasmids were generated using specific primers (sequences available upon request) following the manufacturer’s instructions (QuickChange II, Stratagene). shPLK1 UTR ...
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Molecular Biology 2023Quote: ... megaprimers were synthesized and cloned into the TFAM-mScarlet_pWPXL plasmid following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification of pcDNA3.1-NS3-mCherry-HIS plasmid was done by using PfuUltra HotStart DNA Polymerase (Agilent, Cat #: 600390) and forward and reverse primers (table 2 ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid containing an IDE E111Q mutant was constructed using a QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) and the pProEx-IDE-wt plasmid ...
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bioRxiv - Physiology 2023Quote: ... SIK3-Flag-S884A and S884D plasmids were generated by introducing site-directed mutations into SIK3-Flag plasmid using the QuikChange II XL site-directed mutagenesis kit according to manufacturer’s instruction (Agilent). Lenti-Puro-SIK3-Flag plasmid was constructed by subcloning the “mSIK3-3xFlag” coding sequences (CDS ...
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bioRxiv - Neuroscience 2023Quote: ... nucleotides 214–990) was amplified by PCR and cloned into the pBluscript II KS (−) plasmid (Stratagene, La Jolla, CA). Antisense RNAs were synthesized from linearized plasmids using T3 RNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... The pHD1344tub(PAC)-Nterm3V5-A3 plasmid was mutagenized using the QuickChange Lightning site-directed mutagenesis kit (Agilent, product #210518) and primers listed in Table S2 ...
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bioRxiv - Biochemistry 2023Quote: ... COS1 cells were transfected with pAdTrack-CMV expression plasmids (Table S1) using the GeneJammer transfection reagent (#204130, Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The PAM sequences within each donor plasmid were mutated using site directed mutagenesis with Pfu Turbo (Agilent, Cat# 600250) to avoid targeting of the donor plasmid by Cas9 nuclease ...
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bioRxiv - Immunology 2023Quote: ... SAMD9L-F886Lfs*11-E198A/D243A were generated from pMAX:mTFP1-SAMD9L plasmid using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... a codon-optimized gene block was cloned into the 6xHis_1B LIC plasmid (gift from Scott Gradia, UC Berkeley) and transformed into Arctic Express bacteria (Agilent). Expression was induced with 0.3 mM IPTG for 16 hr at 20°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reference plasmids containing PA tag sequence were constructed in pBluescript II SK (+) vector (Agilent, Palo Alto, CA, USA). One μg of guide RNA inserted vector and 1.0 μg of reference plasmid were co-transfected into EGRG01 embryonic stem (ES ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids for His6-ZZ-TEV-MZT2 and GST-GCP2-NHD were co-transformed into BL21-CodonPlus (DE3)-RIL (Stratagene) cells ...
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bioRxiv - Neuroscience 2023Quote: AAV plasmids carrying cDNA for hIL-6 downstream of a CMV promoter were co-transfected with pAAV-RC (Stratagene) encoding the AAV genes rep and cap ...
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bioRxiv - Genetics 2024Quote: ... the E171Q variant was introduced into an SCN5A expression plasmid (pCDNA3.1) using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent). HEK293T cells were grown at pH 7.4 in filtered sterile DMEM nutrient medium (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... R80A and H71R) or pLRGatewayIRESeYFP-Vpr plasmids were synthesised using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions and primers listed in Table 2 ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids coding for hSSB1 variants were generated from the Y85C substitution-corrected pET29a-hSSB1 vector using the QuikChange (Agilent) mutagenesis kit ...
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bioRxiv - Biophysics 2021Quote: Genes encoding EnHD and CHD in pET24 expression plasmids were described previously.(18) Single residue substitutions were introduced using a Quikchange site-directed mutagenesis kit (Agilent). Genes encoding sequences with multiple substitutions were synthesized by GeneArt (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... variant hLPYK proteins were created by mutating the coding region of the pLC11 plasmid (originally a kind gift from Dr. Andrea Mattevi 28) using QuikChange (Stratagene) and primers designed to individually obtain all 19 substitutions at each position ...
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bioRxiv - Biochemistry 2022Quote: ... The various mutant forms of this plasmid were made with the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent using the appropriate primers each time (Table S4) ...
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bioRxiv - Molecular Biology 2020Quote: Per2AS variant 2 overexpression plasmid was generated from 5’RACE and 3’RACE products that were cloned in to pBluescript (Agilent), as well as Per2AS qPCR product cloned into pGEM-T (Promega) ...