Labshake search
Citations for Agilent :
301 - 345 of 345 citations for RPA reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... in a final volume of 50 μL and were diluted to 1.5 ng uL-1 to be measured in the High-Sensitivity ScreenTape D1000 reagents (Agilent Technologies, Germany). We included blank controls in all laboratory processes together with our samples ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA samples’ quality (purity and integrity) was determined utilizing the Agilent 2100 Bioanalyser with the RNA 600 NanoLabChip reagent set (Agilent Technologies). The RNA was quantified by Qubit fluorometry (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR reactions using SYBRgreen fluorescent reagent and/or EvaGreen™ were achieved on the Stratagene Mx3000P system (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The quality of the libraries and fragment distribution were analyzed using a 4150 TapeStation System and D1000 reagents (Agilent Technologies, USA). Quality control of the purified libraries was performed for up to two weeks before the sequencing run.
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bioRxiv - Neuroscience 2023Quote: ... sections were mounted in glass slides using 0.2% gelatine in TB and cover-slipped with fluorescent mounting medium (FluorSave Tm Reagent; Dako, Glostrup, Denmark).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell plates were incubated with 10µl of Flash detection reagent (#93-0247, DiscoverX) for 30 min and chemiluminescence signals were detected with a microplate reader (Synergy MX, Agilent BioTek). Chemiluminescence data were normalized to the signal of basal vehicle-treated cells and mean chemiluminescence values were converted to concentration-response curves for determination of EC50 and Emax values (GraphPad Prism).
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bioRxiv - Genomics 2023Quote: ... RNA was of a consistently high quality (RIN > 8.0) as determined on an Agilent 2200 TapeStation using high sensitivity RNA reagents (Agilent 5067-5579). Total RNA samples were stored at −80°C until RNA-seq library preparation was performed.
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bioRxiv - Immunology 2023Quote: ... The size of the resulting libraries was determined with the Agilent D1000 reagent kit and Agilent 4200 TapeStation (Agilent Technologies, USA). Pooling was performed automatically using a Tecan Freedom EVO robotic station (Tecan ...
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bioRxiv - Bioengineering 2023Quote: ... slides were counterstained with DAPI (1µg/ml) in PBS for 5 min followed by water washes and cover slipping with fluorescent antifade mounting reagent (DAKO, Agilent).
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bioRxiv - Immunology 2024Quote: ... Stop reagent was added and absorbance was read by the CYTATION Gen5 Microplate reader (BioTek, part of Agilent Technologies, version 1.15) at 405 nm against reagent blank.
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bioRxiv - Microbiology 2024Quote: ... Pre-treatment of tissue sections was carried out by pressure cooking in citrate buffer (pH 6.0) and the EnVision+ Single Reagent (HRP. Mouse, Agilent Technologies, K4001) was used as visualisation system in an autostainer (Autostainer Link 48 ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions employing SYBRgreen fluorescent reagent and/or EvaGreen™ were performed in the Stratagene Mx3000P system (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... DNA was also sized using a Genomic DNA ScreenTape Assay (Catalogue numbers: 5067-5366 [Reagents], 5067-5365 [Screentape]) on the TapeStation 2200 system (Agilent UK) following manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... values of the samples were on average 8.40 SE 0.14 as determined using an Agilent 2100 Bioanalyzer Instrument (G2939BA) with the Agilent RNA 6000 Nano Reagents (Agilent, 5067-1511). The DNA was quantified using a Qubit® 2.0 Fluorometer (Q32866 ...
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bioRxiv - Genomics 2024Quote: ... values of the samples were on average 7.8 SE 0.1 as determined using an Agilent 2100 Bioanalyzer Instrument (G2939BA) with the Agilent RNA 6000 Nano Reagents (Agilent, 5067-1511). RNA was divided into two samples and stored at −80°C ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA libraries were assessed for quality and quantification according to the Chromium Single Cell 3’ Reagent kit v3 user guide using the High Sensitivity D5000 ScreenTape (Agilent 5067-5592) and 2200 TapeStation Controller software (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Library quality was assessed on an Agilent 2100 Bioanalyzer using High Sensitivity DNA reagents and chips (Agilent Technologies, Santa Clara, CA, USA). Library concentration was measured with a Qubit 2.0 Fluorometer using a dsDNA BR Assay Kit (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... and fragment length of our total RNA using a TapeStation 4200 System with RNA ScreenTape and reagents (Agilent, Santa Clara, CA, USA). We prepared barcoded ...
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bioRxiv - Microbiology 2022Quote: ... The quality and quantity of libraries were checked with Agilent TapeStation Systems with High Sensitivity D1000 reagent and screen tape (Agilent #5067-5585 and Agilent #5067-5584). The libraries were pooled for sequencing on Illumina Nextseq instrument or Illumina Novaseq instrument with paired-end reads of 75 bp or 100 bp ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% H2O2 / Methanol for 10 minutes followed by incubation with Dako Dual Endogenous Enzyme Block reagent (Agilent Dako, S2003) for 30 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Pool quality control was performed with an Agilent High Sensitivity D1000 Screen Tape reagent kit using the Agilent 4200 TapeStation (Agilent Technologies, USA). Whole-genome sequencing (WGS ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA library quality and quantity were determined using an Agilent 2100 bioanalyzer using the High Sensitivity DNA Reagents kit (Agilent, 5607-4626) and then sequenced on an Illumina NovaSeq 6000 ...
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bioRxiv - Cancer Biology 2024Quote: ... at 1:400 for two hours and then incubated for 30 minutes with Rabbit Envision HRP System reagent (Agilent, Santa Clara, CA). For vimentin staining ...
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bioRxiv - Cell Biology 2024Quote: ... and RNA quality analyzed using Agilent 2100 Bioanalyzer RNA 6000 Nano Reagent using the Eukaryote Total RNA Nano Series II assay (Agilent Technologies Inc).
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bioRxiv - Cell Biology 2019Quote: ... and cDNA was examined for quality using the Agilent 2200 Tapestation with the High-sensitivity D5000 screentape and reagents (Agilent Technologies, Waldbronn, Germany), and the Qubit® 2.0 Fluorometer and Qubit dsDNA HS Assay Kit (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... concentration and purity were evaluated on an Agilent RNA6000 Nano Chip in Reagent Port 1 of the Bioanalyzer Agilent 2100 (Agilent Technologies, CA, USA). Sequencing of the libraries was done on the Illumina HiSeq 4000 platform by BGI Co. ...
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bioRxiv - Genomics 2020Quote: ... Chromium Genome Reagents Kit Version 2 User Guide) and size and concentration determined using an Agilent 2100 Bioanalyzer DNA 1000 chip (Agilent Technologies, CA, USA). Libraries were then sequenced on an Illumina NovaSeq 6000 System following the manufacturer’s protocols (Illumina ...
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bioRxiv - Systems Biology 2022Quote: DyTo was separated from the unreacted reagents and by-products by mass-directed preparative HPLC (infinity prep II, Agilent Series 1260, LC-MSD) using reversed-phase C18 column (VP10/125 5 μm ...
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bioRxiv - Cell Biology 2019Quote: ... KLF4 and C-MYC open reading frames under the control of the human elongation factor-1α (EF-1α) promoter in the presence of 1.2% GeneJammer (a polyamine-based transfection reagent; Stratagene, La Jolla, CA, USA). Cells were maintained in mTeSR™1 (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin fragment size was confirmed to be 200-500bp on the Agilent 4200 tape-station with HS D1000 tape and reagents (#5067-5584, Agilent Technologies, California, USA). Chromatin immunoprecipitation was performed as previously described previously63–65 with minor modifications ...
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bioRxiv - Microbiology 2021Quote: ... The concentration and quality of 16S rRNA gene amplicons were measured using a TapeStation 4200 system with D5000 tapes and reagents (Agilent Technologies, CA, USA). Equimolar pools of samples were generated before sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were heated with sodium citrate (pH=6) for antigen retrieval and blocked with a Protein Block reagent (Dako, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Purity and integrity of the RNA was assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA).
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bioRxiv - Genomics 2022Quote: ... The obtained library quality and concentration was assessed by monitoring the distribution of the fragment sizes with a Bioanalyzer 2100 instrument using RNAnano reagent kit (Agilent Technologies, Santa Clara, CA). This size and quantity information was used for pooling the libraries in equimolar concentrations to normalize each library ...
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bioRxiv - Immunology 2021Quote: ... The size of the amplicon was confirmed by analyzing 2 μl of PCR products using the Agilent D5000 ScreenTape System (Agilent D5000 ScreenTape, 5067-5588, Agilent D5000, Reagents 5067-5589). Products were quantified by analyzing 2 μl with the Quant-iT dsDNA High-Sensitivity Assay Kit (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... Purity and integrity of the RNA was assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA). The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Purity and integrity of the RNA was assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... slides were incubated with K10 for 30 minutes (32°C) and reactions were visualized using EnVision™ FLEX/HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) following the recommendation of the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... and Ii L17A was subsequently used as template for PCR quick change mutagenesis (all reagents used were included in the kit; QuickChange® Site-Directed Mutagenesis (Stratagene, La Jolla, CA, USA)) in order to generate the AP-3 binding motif RRP 21 ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA quality assessment was conducted by the Utah State University Center for Integrated Biosystems using a TapeStation 2200 with high sensitivity RNA reagents (Agilent Technologies, Santa Clara, CA, USA). Total RNA was sent to the Roy J ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragment size distributions and concentrations of the final libraries were determined using the High Sensitivity D1000 Screen tape assay and reagents (Agilent, 5067-5584 and 5067-5585) on the Agilent 2200 TapeStation System ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA quality and quantity were assessed using an Agilent Tapestation 2200 using the Genomic DNA screentape and reagents (Agilent, Catalogue no. 5067-5365 and 5067-5366). Samples were sonicated custom number of cycles to achieve fragments of uniform length ...
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bioRxiv - Microbiology 2022Quote: ... The quality and quantity of libraries were checked with Agilent TapeStation Systems with High Sensitivity D1000 reagent and screen tape (Agilent #5067-5585 and Agilent #5067-5584). The libraries were pooled for sequencing on Illumina Nextseq instrument or Illumina Novaseq instrument with paired-end reads of 75 bp or 100 bp ...
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bioRxiv - Cell Biology 2020Quote: ... slides were incubated with K10 for 30 minutes (32°C) and reactions were visualized using EnVision™ FLEX/HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) following the recommendation of the manufacturer ...
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bioRxiv - Genomics 2023Quote: ... The concentration and average size of the libraries was verified using the TapeStation DNA 5000 Assay Reagents and Screen Tapes (Agilent, Cat. # 5067-5589 and 5067-5588). Libraries were normalized and pooled based on the TapeStation concentrations ...