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Citations for Agilent :
251 - 300 of 345 citations for RPA reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... The Seahorse ATP rate assay was run according to manufacturer’s instruction and all reagents for the Seahorse assays were sourced from Agilent Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... The Seahorse ATP rate and cell energy phenotype assays were run according to manufacturer’s instruction and all reagents for the Seahorse assays were sourced from Agilent Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... The nonspecific-binding sites on sections were then blocked using Protein Block Serum-Free reagent (Dako North America Inc.) for 10 minutes at room temperature ...
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bioRxiv - Genomics 2022Quote: ... we utilized a slow-hybridization protocol involving hybridization reagents from a SureSelect XT0 kit (Agilent Technologies, Santa Clara, CA). Following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... COS1 cells were transfected with pAdTrack-CMV expression plasmids (Table S1) using the GeneJammer transfection reagent (#204130, Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... according to the manufacturer’s protocols using the D5000 screentape and reagents for cDNA QC (Agilent, 5067-5588, 5067-5589) and D1000 screentape and reagents for final library QC (Agilent ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated with the indicated primary antibodies (Reagents) overnight at 4 °C followed by Envision/diaminobenzidine detection (Dako, Glostrup, Denmark) and hematoxylin counterstaining/mounting (Entellan ...
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bioRxiv - Molecular Biology 2020Quote: ... Final library yields and size were measured on a Bioanalyzer 2100 using high sensitivity reagents (Agilent Technologies, Supplementary Figure 8).
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bioRxiv - Genomics 2020Quote: ... Insert sizes and library concentrations of final libraries were determined using an Agilent 2100 bioanalyzer instrument (Agilent DNA 1000 Reagents) and real-time quantitative PCR (TaqMan Probe ...
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bioRxiv - Genetics 2019Quote: ... Isolated RNA was reverse transcribed into cDNA was by Prime Script TM RT reagent Kit with gDNA Eraser (Stratagene, Takara). Real-time PCR was performed on ABI-7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... Sample input was 900ng total RNA (determined by Qubit RNA HS reagents, Thermo) with a RIN >8 (TapeStation High Sensitivity RNA ScreenTape Assay, Agilent). RNA was fragmented for 15 minutes and libraries were constructed with a 1/5th dilution of NEB adaptor and 12 cycles of PCR amplification with dual-indexing primers ...
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bioRxiv - Plant Biology 2019Quote: ... Purity and integrity of total RNA was assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent). RNA extraction from egg cells was carried out at the genomics core facility of the University of Regensburg ...
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bioRxiv - Microbiology 2020Quote: Huh7.5 cells were seeded 16h prior to transfection with pEGFP-N3-VSV-Gts and p7-encoding plasmid using GeneJammer transfection reagent (Agilent). Medium was changed 4h post-transfection and cells were incubated overnight at 40°C ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 3X 5 mins in 1X PBS and incubated in secondary antibody diluted in DAKO antibody diluent reagent with background reducing components (DAKO) for 1 hour at room temperature with gentle agitation on a shaker (See Table S4 for antibody information) ...
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bioRxiv - Microbiology 2021Quote: ... Tissue samples were stained with hematoxylin and eosin or immunostained to detect SARS-CoV-2 antigen using VIDO-InterVac’s polyclonal rabbit anti-SARS-CoV-2 AS20-014 (1:400 dilution) and detected using an HRP-labelled polymer detection reagent (EnVision+ System – HRP Labelled Polymer, Agilent Technologies Canada Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... sample input was 400 ng total RNA (determined by Qubit RNA HS reagents, Thermo) with RIN >7 (Bioanalyzer RNA Nano, Agilent). Libraries were prepared with the Biomek 400 Automated Workstation (Beckman Coulter) ...
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bioRxiv - Neuroscience 2022Quote: ... and quality assessed by High Sensitivity D5000 ScreenTape (5067-5592) with High Sensitivity D5000 Reagents (5067-5593) on the 2200 TapeStation system (Agilent). The WTA (whole transcriptome amplified ...
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bioRxiv - Genomics 2019Quote: ... The libraries were amplified and capture was performed using the RNA baits described above using the SureSelect reagents and protocol (Agilent). Following a second amplification the libraries were sequenced using paired-end Illumina SBS technology (see Supplementary Methods for details).
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bioRxiv - Genomics 2019Quote: ... Amplicons were isolated on a 2% TAE agarose gel and bands were confirmed using Agilent’s D1000 ScreenTape and reagents (Agilent Technologies) on a 2200 Tapestation system ...
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bioRxiv - Cell Biology 2020Quote: ... After a cleanup with SPRIselect Reagent Kit and fragment size estimation with High SensitivityTM HS DNA kit runned on 2100 Bioanalyzer (Agilent), the libraries were constructed by performing the following steps ...
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bioRxiv - Cancer Biology 2020Quote: ... Library quality and quantity was assessed on a Bioanalyzer using the DNA High Sensitivity Kit reagents and protocol (Agilent Technologies). The quantified libraries were pooled together at a final concentration of 100pM ...
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bioRxiv - Biochemistry 2021Quote: ... incubated with Dako biotin (cat. no. X0590) and peroxidase (cat. no. S200389) blocking reagents (both Agilent, Santa Clara, CA, USA), and incubated with primary antibodies (diluted in TBS-T-BSA ...
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bioRxiv - Immunology 2021Quote: ... Libraries size was determined using the Agilent D1000 ScreenTape System (Agilent D1000 ScreenTape, 5067-5582, Agilent D5000 Reagents, 5067-5583) and quantified with the Quant-iT dsDNA High-Sensitivity Assay Kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Insert sizes and the concentration of final libraries were determined using an Agilent 2100 bioanalyzer instrument (Agilent DNA 1000 Reagents) and real-time quantitative PCR (TaqMan Probe ...
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bioRxiv - Genetics 2020Quote: Quantitative RT-PCR was performed on MYB5a and MYB5b using SYBR-Green Brilliant III Ultra-Fast reagents (Agilent Technologies, USA), in optical 96-well plates (Greiner Bio-One ...
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bioRxiv - Cancer Biology 2020Quote: ... The quality and size distribution of pooled libraries were evaluated on an Agilent 2100 Bioanalyzer using High Sensitivity DNA reagents (Agilent).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were stained with CellROX reagents (Green) and Hoechst 33342 was used as nuclear stain and viewed in Cytation cell imaging multimode reader (Agilent) immediately ...
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bioRxiv - Developmental Biology 2023Quote: ... Quality of the final library preparation was analysed on the Bioanalyzer using the High Sensitivity DNA reagents kit and cassettes (5067-4626, Agilent). For all the steps involving purification of library intermediates ...
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bioRxiv - Genomics 2023Quote: ... Hybrid capture of exonic regions was performed using Agilent SureSelect in-solution capture reagents and SureSelect XT Human All Exon V4 probes (Agilent). Captured libraries were sequenced using 100 bp paired-end runs on Illumina HiSeq 2500 ...
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bioRxiv - Cell Biology 2023Quote: ... GEMs were used to generate barcoded cDNA libraries following the manufacturer’s protocol (Single Cell 3’ Reagent Kit v3.1, 10X Genomics) and quantified using the TapeStation High Sensitivity D5000 kit (Agilent, Germany). Subsequently ...
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bioRxiv - Genomics 2023Quote: ... The extent of fragmentation of DNA was assessed using an Agilent TapeStation system with Genomic DNA ScreenTape reagents (Agilent Technologies). All DNA samples had a DNA integrity number (DIN ...
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bioRxiv - Cancer Biology 2023Quote: ... libraries were constructed and sequenced similarly to the WGS experiment following hybrid capture selection with the hybrid reagent SureSelect DNA - Mouse All Exon V1 (Agilent). WES reads were aligned to the GRCm38 reference genome using BWA-MEM ...
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bioRxiv - Cancer Biology 2023Quote: ... tissue was permeabolized with 0,5% Triton X-100 in PBS (PBS-T) and non-specific binding was blocked by incubating the tissue section with blocking reagent (DAKO). Antibody staining was performed in PBS-T with 1% blocking reagent (DAKO ...
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bioRxiv - Bioengineering 2023Quote: ... slides were counterstained with DAPI (1µg/ml) in PBS for 5 min followed by water washes and cover slipping with fluorescent antifade mounting reagent (DAKO, Agilent).
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of the amplified barcode product in each PCR was measured using D1000 screentape reagents (5067-5582, Agilent Technologies) and the Tapestation instrument (Agilent Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR was performed with primers (Table S2) and Brilliant Sybr Green QPCR Reagent Kit (Stratagene, Waldbronn, Germany). The ratio of G ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA concentration and sample quality by RNA integrity number (RIN) were checked using Tapestation RNA ScreenTape reagents (Agilent cat.# 5067-5576). cDNA libraries were prepared with the SureSelect Strand-Specific RNA Library Prep Kit (Agilent cat.#G9691B ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA yield and purity were both assessed using the Agilent RNA 6000 Pico Reagents with the Agilent 2100 Bioanalyzer System (Agilent Technologies). RIN scores were between 7.7 and 8.6 for all samples ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentration was then quantified at The Center for Functional Genomics/University at Albany using an Agilent Technologies 2100 Bioanalyzer System (Santa Clara, CA) and respective Bioanalyzer Small RNA reagents (catalog no. 5067-1548, Agilent Technologies). Purified small RNA samples were then digested using Nucleoside Digestion Mix (catalog no ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% H2O2 / Methanol for 10 minutes followed by incubation with Dako Dual Endogenous Enzyme Block reagent (Agilent Dako, S2003) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... For secondary antibody staining, either ImmPRESS kits (anti-goat, MP-7405; anti-rat, MP-7444) or Envision+ Reagents (Dako: rabbit, K4002) were used ...
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bioRxiv - Neuroscience 2022Quote: ... Each library was measured for size with Agilent’s High Sensitivity D1000 ScreenTape and reagents (Agilent, Cat. No. 5067-5584 & 5067-5603) and concentration with KAPA SYBR FAST Universal qPCR Kit (Kapa Biosystems ...
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bioRxiv - Immunology 2021Quote: ... PCR amplification verification and library validation was done using a TapeStation electrophoresis system and D1000 ScreenTape reagents (Agilent, Santa Clara, California). Libraries were quantified using a Qubit 4 Fluorometer and the Qubit dsDNA HS assay kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... The sections were then treated with envision FLEX/HRP reagent for 20 min and then washed and stained by the envision FLEX-DAB chromogen (cat No. DM827, Dako, Denmark) and by Mayer’s Hematoxylin (Lille’s Modification ...
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bioRxiv - Molecular Biology 2019Quote: ... Each Hi-C library (750 ng) was hybridized and captured individually using the SureSelectXT Target Enrichment System reagents and protocol (Agilent Technologies). After library enrichment ...
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bioRxiv - Cancer Biology 2020Quote: ... total RNA was isolated with the Trizol reagent from fresh frozen tissue and RNA integrity was checked with the Bioanalyzer 2100 (Agilent Technologies). For cDNA library preparation the TruSeq Stranded Total RNA sample preparation kit (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Quality control and quantification of the libraries were obtained on an Agilent 2100 Bioanalyzer System using the High Sensitivity DNA reagents and DNA chips (Agilent Genomics). Metagenomic libraries were diluted to 1 nM using the Select-a-Size DNA Clean and Concentrator MagBead Kit (Zymo Research ...
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bioRxiv - Genomics 2020Quote: ... Hi-C DNA up to 750 ng was concentrated using a vacuum concentrator and bound to the capture baits in a single hybridisation reaction using SureSelectXT reagents and protocol (Agilent Technologies). The biotinylated baits were captured using Dynabeads MyOne Streptavidin T1 beads (Life Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... The culture medium was replaced with MAS buffer containing 10 nM XF plasma membrane permeabilizer (PMP) reagent (Seahorse Bioscience 102504–100) with 5 mM malate + 5 mM pyruvate (for complex I assays ...