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Citations for Agilent :
301 - 350 of 586 citations for 4 Keto 13 cis retinoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The upper ether layer was collected and SCFA content was analyzed on an Agilent 7890B gas chromatography system with flame ionization detector using a high-resolution capillary column for detection of volatile acids (DB-FFAP, 30 m x 0.25 mm with 0.25 μm film thickness; Agilent Technologies, Santa Clara, CA). A standard solution containing 10 mM of acetic ...
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bioRxiv - Plant Biology 2020Quote: ... the HY5 36th Serine (AGC) was changed into Alanine (GCC) and Aspartic Acid (GAC) respectively using Quickchange II site-directed mutagenesis kit (Agilent, Catalog #200523) and cloned into pB7FWG vectors ...
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bioRxiv - Biochemistry 2021Quote: ... The digestion and desalting (total time 3 min) were driven by 0.4% formic acid (FA) in water pumped by 1260 Infinity II Quaternary pump (Agilent Technologies, Waldbronn, Germany) at a flow rate of 100 μL·min−1 ...
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bioRxiv - Microbiology 2020Quote: Each ELV and MV preparation were reconstituted in 10 μl of 5% formic acid and injected onto a 50 mm 300 μm C18 trap column (Agilent Technologies, USA). The samples were then desalted for 5 min at 30 μl/min using 0.1% formic acid and the peptides were then eluted onto an analytical nano-HPLC column (150 mm × 75 μm 300SBC18 ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of total RNA was measured on a Nanodrop device and the quality of the extracted nucleic acid was assessed using a Bio-analyzer 2100 (Agilent technologies, CA). Microarray probes were synthetized in one cycle of RNA amplification in which molecules were labeled (Affymetrix microarray station ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentration of total RNA was measured using a Nanodrop spectrophotometer and the quality of the extracted nucleic acid was assessed on a Bio-analyzer 2100 (Agilent Technologies, CA). Microarray probes was synthetized by one cycle of RNA amplification in which molecules were labeled in an Affymetrix microarray station (Affymetrix ...
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bioRxiv - Systems Biology 2022Quote: ... the RNA integrity number was 10 as assessed by the Stanford Protein and Nucleic Acid (PAN) Biotechnology Facility using the RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-SΔ19 expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) by following the manufacturer’s instructions and using mutagenic oligonucleotides SaCoV2-K417T-F ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were resuspended in hexane and fatty acids were separated with gas chromatography (8860 or 7890A GC system, Agilent Technologies, CA, USA) combined with mass spectrometry (5977B or 5975C Inert MS system ...
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bioRxiv - Physiology 2023Quote: ... High-resolution gas chromatography capillary column coated with 0.25µm film thickness was used (DB-FFAP) for the detection of volatile acids (Agilent Technologies, Santa Clara, CA). The oven temperature was 145°C and the FID and injection port were set to 225°C ...
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bioRxiv - Microbiology 2023Quote: ... Elemental quantification on acid-digested liquid samples was performed using an Agilent 7700 inductively coupled plasma mass spectrometer (Agilent, Santa Clara, CA). The following settings were fixed for the analysis Cell Entrance = −40 V ...
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bioRxiv - Cancer Biology 2022Quote: ... RAS double mutants with point-mutations in the 95th and 96th amino acid residues were generated by site-directed mutagenesis (Agilent Technologies #200521) using the primers listed in Supplementary Table 2 per manufacturer’s recommended PCR conditions ...
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bioRxiv - Biophysics 2024Quote: ... that is loaded onto a Waters HDX-Manager with a desalting flow of 0.23% aqueous formic acid solution (Solvent A) provided by an Agilent 1260 Infinity quaternary pump (Agilent Technologies, CA, USA) and a gradient flow comprised of Solvent A (0.23% aqueous FA solution ...
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bioRxiv - Microbiology 2024Quote: ... Elemental quantification on acid-digested liquid samples was performed using an Agilent 7700 inductively coupled plasma mass spectrometer (Agilent, Santa Clara, CA). The following settings were fixed for the analysis Cell Entrance = −40 V ...
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bioRxiv - Cell Biology 2024Quote: ... We mutated the Serine 69 to Alanine (69A) or aspartic acid (69D) using the QuickChange II Site-directed Mutagenesis kit (Agilent Technologies, #200523). The primer for mutating S69-A was ...
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bioRxiv - Plant Biology 2022Quote: ... and hybridized to a rice 4 × 44 K custom oligo DNA microarray (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... A C18 reversed phase column (Poroshell 120 EC-C18, 4 μm, 4.6x150 mm, Agilent) was used for the separation ...
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bioRxiv - Plant Biology 2021Quote: ... Arabidopsis Gene Expression Microarray chip version 4 with 43,803 Arabidopsis probes (G2519F; Agilent Technologies) was used for the microarray analysis ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were diluted 4 times in water and ran on a Bioanalyzer (Agilent Technologies) to determine cDNA concentration ...
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bioRxiv - Immunology 2020Quote: ... sections were probed with an anti-HSV antibody incubated overnight at 4°C (Dako), a donkey anti-rabbit IgG-HRP antibody (Jackson Immunoresearch ...
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bioRxiv - Molecular Biology 2024Quote: ... pancreatic sections were stained with the following antibodies: anti-insulin (Dako IR002; 1:4), anti-glucagon (Santa Cruz ...
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bioRxiv - Immunology 2024Quote: ... cells were treated at different time points with Etomoxir (4 µM; 103672-100; Agilent), Oligomycin (1.5 µM ...
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bioRxiv - Neuroscience 2024Quote: ... 4 mM ADP and 1 nM plasma membrane permeabilizer (Agilent Technologies, Cat #102504-100). Cells were incubated for 20 minutes in MAS ...
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bioRxiv - Pathology 2020Quote: ... resuspended to 0.5 µg/µL in 90% ACN/0.1% trifluoroacetic acid (TFA) and fractionated with an Agilent 1200 high performance liquid chromatography (HPLC) system (Agilent Technologies, Santa Clara, CA) on an in-house 17 cm TSKgel Amide-80 HILIC column (3 µm particle size ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-Strunc expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) using mutagenic oligonucleotides as follow:
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bioRxiv - Immunology 2022Quote: FFPE tissue sections were deparaffinized and antigen retrieval was undertaken in 0.1 M citric acid buffer solution (Dako, target retrieval solution, S169984-2) using an electric pressure cooker (Russell Hobbs RHNHP401) ...
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bioRxiv - Physiology 2021Quote: ... Amino acid substitution mutants were generated by site-directed mutagenesis using a QuickChange site-directed mutagenesis kit (Agilent Technologies, La Jolla, CA, USA). The predicted DNA sequences of all constructs were verified by sequencing.
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Bioengineering 2023Quote: ... An aliquot of 1 mL was obtained and diluted with 2% nitric acid before running for elemental analysis using an inductively coupled plasma optical emission spectrometer (ICP-OES, Varian 720-ES, Agilent, Santa Clara, CA). For each run ...
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bioRxiv - Molecular Biology 2020Quote: ... and crosslinking was performed at 100mJ/cm2 at 4°C in a Stratalinker 1800 (Stratagene). Crosslinked cells where harvested by scraping in cold 1xPBS and pelleted at 700xg for 2min ...
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bioRxiv - Cancer Biology 2020Quote: ... Each section was treated with 4 drops of serum-free protein block (Agilent cat # X0909) for 20 minutes at room temperature ...
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bioRxiv - Genetics 2022Quote: ... the DNAs were hybridized on CGH 4 × 180 K mouse slides (AMADID 027411, Agilent Technologies). Finally ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated overnight at 4°C and appropriate HRP-conjugated secondary antibodies (DAKO, Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA from 4 independent replicates were checked on RNA6000 RNA chips on Bioanalyzer (Agilent) for its quality and integrity ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... they were stored in 4 mL clear glass vials with polytetrafluoroethylene-lined caps (Agilent, USA), and measurements were performed at 2 ...
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bioRxiv - Plant Biology 2020Quote: ... 4 μg of total RNA with an RNA Integrity Number (RIN) ≥ 8 (Bioanalyzer 2100, Agilent Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... and incubated overnight at 4 °C with primary antibodies: Anti-GFAP 1:1000 (Z0334, Dako), Anti-CD68 1:400 (MCA1957 ...
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bioRxiv - Plant Biology 2021Quote: ... Hybridizations were done using a custom 4×44 k oligoarray (Agilent Technologies, St. Clara, USA) previously described (Lembke et al. ...
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bioRxiv - Immunology 2021Quote: ... Approximately 4 × 104 ILC2s re-suspended in 50 μL Seahorse XF RPMI pH 7.4 (Agilent) (+ 1M glucose ...
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bioRxiv - Molecular Biology 2023Quote: ... on a Qubit 4 fluorometer and a HS NGS Fragment kit (Agilent: DNF-474-1000). Libraries were normalized and pooled to 2nM by calculating the concentration based off the fragment size (base pairs ...
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bioRxiv - Microbiology 2023Quote: ... where non-specific binding cites were blocked with 4% normal rabbit serum (X0902; Dako, DK), the primary antibody was a polyclonal goat anti-IBA1 (ab5076 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed with PBS and stored at 4°C in Mounting medium (Dako).
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4°C overnight with primary antibody in Protein Block (Dako, CA). Sections were rinsed 4x in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... with α-fucosidase (bovine kidney from Sigma-Aldrich or almond α1,3/4-specific from Prozyme), α-mannosidase (jack bean from Sigma) ...
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bioRxiv - Physiology 2022Quote: ... Slides were incubated overnight at 4°C in rabbit anti-GFAP (Agilent Cat# Z0334, RRID:AB_10013382) at a dilution of 1:2000 in antibody diluent (Agilent Cat# S0809) ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons (each 3-4 kbs in length) were generated via PCR using EasyA polymerase (Agilent) and Sanger sequenced.
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bioRxiv - Microbiology 2021Quote: ... and branched-chain amino acids (BCAAs) was performed with an Agilent 7890A gas chromatograph coupled with an Agilent 5975 mass spectrometer (Agilent Technologies Santa Clara, CA) using a propyl esterification method as previously described [61] ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were loaded with solvent A (0.1% formic acid in water) onto an in-house packed analytical column filled with Poroshell EC120 C18 material (Agilent Technologies, Santa Clara, CA, USA). Peptides were chromatographically separated at a constant flow rate of 250 nL/min using the following gradient ...
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bioRxiv - Cell Biology 2022Quote: ... were diluted with ultra-pure water to reduce the acid concentration below 3% and loaded into the ICP-MS-MS (triple quad Agilent 8800x ICP-MS-MS). The instrumental conditions were optimized to remove interferences by using a collision/reaction cell ...