Labshake search
Citations for Agilent :
501 - 550 of 586 citations for 4 Keto 13 cis retinoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
-
bioRxiv - Biochemistry 2020Quote: ... SDM was carried out with appropriate primers (Table 4) using QuikChange® Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) according to manufacturer’s instruction.
-
bioRxiv - Cell Biology 2021Quote: Primary SVF cells from BAT and sWAT were isolated and cultured for 4 days before being plated in XF cell culture microplates (Seahorse Bioscience). SVF cells (10,000 cells ...
-
bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed with 4 μl Brilliant III Ultra-Fast SYBR QPCR as instructed by the manufacturer (Agilent Santa Clara). All primers for quantification of either expression of viral m/cRNAs or antiviral responses are shown below.
-
bioRxiv - Biochemistry 2020Quote: ... 100 μl of 2 – 4 mg/ml protein samples were applied to a column using the 1260 Infinity HPLC system (Agilent Technologies) coupled to a MiniDawn Treos detector (Wyatt Technologies ...
-
bioRxiv - Genetics 2022Quote: ... and incubated in the dark overnight at 4 C before running on a Novocyte Quanteon flow cytometer using NovoExpress 1.3.0 software (Agilent, Santa Clara, CA) at a flow rate of 14 uL/minute ...
-
bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated overnight at 4°C and appropriate HRP-conjugated secondary antibodies (DAKO, Agilent Technologies, Santa Clara, CA, USA) for 1 hour at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... at 4°C overnight followed by incubation with Dako EnVision+System-HRP Labelled Polymer Anti-Mouse (Aglient Dako, Cat # K400111-2) for 60 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... The Samples were diluted 4 fold with water and 1 μl supernatant was loaded on a Bioanalyzer High Sensitivity DNA electrophoresis chip (Agilent Technologies).
-
bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...
-
bioRxiv - Biochemistry 2021Quote: ... or PDK1SKD-PIF S241A (4 mg/ml) was injected onto a Superdex 200 10/300 column (Cytiva) operated by a 1260 Infinity HPLC (Agilent Technologies). Light scattering of a 690 nm laser was detected by a MiniDawn Treos (Wyatt ...
-
bioRxiv - Developmental Biology 2021Quote: ... for 6 minutes (30 seconds ON, 30 seconds OFF) at 4°C to obtain ~200 bp fragments (confirmed using an Agilent Bioanalyzer). Sonicated DNA was extracted using ethanol precipitation ...
-
bioRxiv - Cell Biology 2020Quote: ... All hESC lines were analysed for their genetic content by array-based comparative genomic hybridization (Human Genome CGH Microarray 4×44K, Agilent Technologies) as previously described (Jacobs et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in blocking solution (DAKO, Mississauga, Ontario, Canada). Sections were incubated overnight in polyclonal rabbit anti-GFAP primary antibody and monoclonal mouse anti-tau antibody (DC190) ...
-
bioRxiv - Cancer Biology 2022Quote: Genome-wide copy number variations were assessed using the SurePrint G3 Human aCGH 4×180K Microarray (Agilent Technologies, Santa Clara, CA), according to the manufacturer’s recommendations ...
-
bioRxiv - Bioengineering 2024Quote: ... Bevacizumab-sensitive/resistant U87 cells were cultured identically to wild-type U87 cells.79 Cells were screened for mycoplasma every 3 – 4 months with the MycoSensor qPCR Assay Kit (Agilent Technologies).
-
bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were post-fixed with 4% formaldehyde for 10 minutes and histologically stained with Dako Mayers hematoxylin (Agilent, cat. no. S3309) (4 minutes ...
-
bioRxiv - Immunology 2024Quote: A total of 10 µg/ml rHA were coated on an ELISA plate at 4 °C ON and afterwards incubated with rabbit anti-HA antibody (1:2000 dilution) (Dako, A0001) 2h at RT ...
-
bioRxiv - Immunology 2023Quote: ... pH 7.4) for 1 h without CO2 prior to measurement of O2 consumption and extracellular acidification rate (ECAR) by XFe24 (Seahorse Bioscience) with sequential addition of 1 μM of oligomycin ...
-
bioRxiv - Cancer Biology 2023Quote: The 4-6um thick TMA sections were deparaffinized and antigens were unmasked using EnVision FLEX Target Retrival Solution Low pH (Dako Agilent) in PT link device (PT200 ...
-
bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
-
bioRxiv - Neuroscience 2023Quote: ... for 60 min and incubated overnight at 4°C with primary antibodies diluted in DAKO Antibody Diluent (Agilent, cat. #S080983-2). Sections were washed in PBS (3 x 5 min ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in a blocking solution (DAKO, Ontario, Canada). Sections were incubated overnight in a polyclonal rabbit anti-IBA-1 primary antibody (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... solution (pH 7.4) and incubated with goat anti-mouse or anti-rabbit immunoglobulin labeled with dextran molecules and horseradish peroxidase (EnVision, Dako Omnis) at room temperature for 30 min ...
-
bioRxiv - Genomics 2023Quote: Each purified GST-tagged DBD stock was validated for DNA-binding specificity using 4×44k universal protein-binding microarrays (PBMs) (Agilent Technologies), as described previously42 ...
-
bioRxiv - Bioengineering 2023Quote: ... equipped with a UV detector through a C18 column Poroshell 120 EC-C18 (4.6 × 150 mm, 4 μm) (Agilent Technologies, Inc.). Mobile phase consisted of acetonitrile/methanol/0.4% acetic acid/ tetrahydrofuran (80:12:5:3 ...
-
bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and the OD600 was recorded every hour for 24 hours using a stacker (Biostack 4, Agilent BioTek) and plate reader (Epoch 2 ...
-
bioRxiv - Immunology 2024Quote: ... the cells were washed once with PBS-EDTA and fixed with 1% paraformaldehyde before FACS analysis with a 4-laser (405, 488, 561 and 637 nm) Quanteon Novocyte (Agilent Technologies).
-
bioRxiv - Neuroscience 2024Quote: ... then stained with DAPI for 10 minutes at room temperature followed by 4 x 5-minutes washes in 1X PBS before being mounted using antifade fluorescence mounting media (Dako, S3023).
-
bioRxiv - Cell Biology 2024Quote: Cy3-labeled cRNAs were further hybridized onto Agilent Whole Human Genome kit 4 x 44K slides (Catalog number G4112F, Agilent Technologies) containing 44,397 oligonucleotide probes in 2X Hi-RPM Hybridization buffer (Catalog number 5188-5242 ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in primary antibodies overnight at 4 °C (Figure S2) and in secondary antibody-horseradish peroxidase complex (REAL EnVision detection kit, Dako #K5007) for 1 h at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... After incubation the cells were fixed in 4 % PFA and stained with rabbit anti human C3c FITC (1: 100 Dako F020102) antibody ...
-
bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and incubated at 37 °C for 24 h in a stacker-incubator system (Biostack 4, Agilent BioTek) connected to a plate reader (Epoch 2 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and the stock solution was stored anaerobically at 4°C after its concentration was verified using inductively coupled plasma mass spectrometry (ICP-MS; Agilent 7800). Negligible oxidation of arsenite to arsenate was confirmed prior to use by high-performance liquid chromatography (HPLC ...
-
bioRxiv - Plant Biology 2021Quote: ... needles and green stem) of 4 individual clonal Pinus radiata trees using the Plant RNA Isolation Mini Kit (Agilent, Santa Clara, CA). 50 mg of plant material from each tissue type were used for the RNA extractions ...
-
bioRxiv - Neuroscience 2020Quote: ... age range 4-14 years) were acquired using a 7T magnet with an Agilent DirectDrive console (Agilent Technologies, Santa Clara, CA, USA). A 2D diffusion-weighted spin-echo protocol was implemented with a single line readout (DW-SEMS ...
-
bioRxiv - Neuroscience 2020Quote: ... blocked using 10% Normal Goat Serum (NGS) and incubated overnight with the following primary antibodies in 5% NGS overnight at 4°C: rabbit anti-GFAP (DAKO; #Z0334;1:1000), rat anti-L1-CAM (Millipore ...
-
bioRxiv - Microbiology 2021Quote: ... for 1 hour at room temperature or overnight at 4°C using the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) technique according to the manufacturer’s protocol (Dako, Carpinteria, CA, USA). All antibodies were diluted in TBS with 0.1% BSA ...
-
bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...
-
bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...
-
bioRxiv - Microbiology 2020Quote: ... p-nitrophenyl-β-D-xylopyranoside and p-nitrophenyl-α-D-xylopyranoside were carried out by estimating the release of 4-nitrophenol (pNP) at 405 nm using a UV-Vis spectrophotometer (Agilent, G 68608) as described elsewhere (11).
-
Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
-
bioRxiv - Genetics 2023Quote: ... Kallisto quant settings were adjusted to -b 5 -l 160 -s 20 - -single - -threads 4 based on fragment lengths determined by the Agilent 4200 TapeStation (Agilent Technologies, Inc.)
-
bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
-
bioRxiv - Molecular Biology 2022Quote: ... Adapter dilution (1:4) and PCR amplification (26 cycles) were optimized in a pilot experiment using Bioanalyzer DNA1000 (Agilent, Sant Clara, USA) chips as a read out of library size and quantity ...
-
bioRxiv - Cell Biology 2024Quote: ... and HP-0921 from Agilent (m/z 922.0098) in positive mode and 4-NBA (m/z 166.0146) and HP-0921 from Agilent (m/z 966.0007, formate adduct) in negative mode.
-
bioRxiv - Systems Biology 2024Quote: ... The sequencing libraries were quantified using the Qubit 4 fluorometer and library sizes were quantified using the D1000 ScreenTape assay (Agilent 5067-5582) on the TapeStation 4200 automated electrophoresis platform.
-
bioRxiv - Biophysics 2024Quote: A 60,000 member tiling library of the Disprot database (release 2022 12) containing 16 residue-long peptides with 4-fold overlap was ordered from Agilent (G7270A, USA). The library was solubilised in 40 µL TE buffer yielding ∼1 µM concentration ...
-
bioRxiv - Bioengineering 2024Quote: ... 12 by PCR using primers 3 and 4 listed in Supplementary Table 1 and cloned into the BamHI-KpnI site of pBluescript II KS (+) (Stratagene, CA, USA) using ligation mix (Takara Bio USA) ...