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Citations for Agilent :
301 - 350 of 739 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 13C NMR spectrum was recorded on a Varian 500 MHz spectrometer equipped with a 5 mm 1H{13C} XSens cold probe (Varian Inc., Palo Alto, CA, USA, now Agilent Technologies). NMR spectra were referenced to the solvent signals (CHD2OD ...
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bioRxiv - Plant Biology 2021Quote: ... needles and green stem) of 4 individual clonal Pinus radiata trees using the Plant RNA Isolation Mini Kit (Agilent, Santa Clara, CA). 50 mg of plant material from each tissue type were used for the RNA extractions ...
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bioRxiv - Neuroscience 2020Quote: ... age range 4-14 years) were acquired using a 7T magnet with an Agilent DirectDrive console (Agilent Technologies, Santa Clara, CA, USA). A 2D diffusion-weighted spin-echo protocol was implemented with a single line readout (DW-SEMS ...
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bioRxiv - Neuroscience 2020Quote: ... blocked using 10% Normal Goat Serum (NGS) and incubated overnight with the following primary antibodies in 5% NGS overnight at 4°C: rabbit anti-GFAP (DAKO; #Z0334;1:1000), rat anti-L1-CAM (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... for 1 hour at room temperature or overnight at 4°C using the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) technique according to the manufacturer’s protocol (Dako, Carpinteria, CA, USA). All antibodies were diluted in TBS with 0.1% BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...
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bioRxiv - Microbiology 2020Quote: ... p-nitrophenyl-β-D-xylopyranoside and p-nitrophenyl-α-D-xylopyranoside were carried out by estimating the release of 4-nitrophenol (pNP) at 405 nm using a UV-Vis spectrophotometer (Agilent, G 68608) as described elsewhere (11).
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bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... Adapter dilution (1:4) and PCR amplification (26 cycles) were optimized in a pilot experiment using Bioanalyzer DNA1000 (Agilent, Sant Clara, USA) chips as a read out of library size and quantity ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Genetics 2023Quote: ... Kallisto quant settings were adjusted to -b 5 -l 160 -s 20 - -single - -threads 4 based on fragment lengths determined by the Agilent 4200 TapeStation (Agilent Technologies, Inc.)
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bioRxiv - Cell Biology 2024Quote: ... and HP-0921 from Agilent (m/z 922.0098) in positive mode and 4-NBA (m/z 166.0146) and HP-0921 from Agilent (m/z 966.0007, formate adduct) in negative mode.
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bioRxiv - Systems Biology 2024Quote: ... The sequencing libraries were quantified using the Qubit 4 fluorometer and library sizes were quantified using the D1000 ScreenTape assay (Agilent 5067-5582) on the TapeStation 4200 automated electrophoresis platform.
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bioRxiv - Biophysics 2024Quote: A 60,000 member tiling library of the Disprot database (release 2022 12) containing 16 residue-long peptides with 4-fold overlap was ordered from Agilent (G7270A, USA). The library was solubilised in 40 µL TE buffer yielding ∼1 µM concentration ...
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bioRxiv - Neuroscience 2021Quote: All MRI and 1H MRS data were acquired in a 9.4 T Varian Direct Drive system (Agilent Technologies, Santa Clara, CA, USA). A 14 mm diameter receive-only surface RF coil (Doty Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked for with 5% BSA for 1h prior to immunofluorescence staining performed as in (Clément et al., 2018) with PCNA antibody (DAKO, M879, 1:1000). We detected EdU using the Click-iT EdU Cell Proliferation kit as described in (Gatto et al. ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... Chromagen was developed using the DAB (3, 3-diaminobenzidine) reagent (Dako). Sections were counter-stained using pre-filtered Mayer’s haematoxylin (Amber Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Antigen–antibody complexes were revealed with 3-3’-diaminobenzidine (K3468, Dako). Sections were counterstained with haematoxylin (Dako ...
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bioRxiv - Cell Biology 2020Quote: ... Antigen-antibody complexes were revealed with 3-3’-diaminobenzidine (K3468, Dako). Sections were counterstained with hematoxylin (Dako ...
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bioRxiv - Physiology 2022Quote: ... Antigen–antibody complexes were revealed with 3-3′- diaminobenzidine (K346811, Dako) or the DAB (Polymer ...
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bioRxiv - Cell Biology 2024Quote: ... at 1:500 and 3-3’-diamino-benzidine-tetrahydrochloride (Dako, K3468). Stained sections were imaged using an NanoZoomer 2.0HT (Hamamatsu).
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bioRxiv - Cancer Biology 2024Quote: ... Antigen–antibody complexes were reveled with 3-3′-diaminobenzidine (K346811, Agilent). Sections were counterstained with hematoxylin (CS700 ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was harvested by centrifugation at 1500 rcf for 4 minutes at 23°C and filtered using a Captiva 96 well 0.2 μm filter plate (Agilent Technologies, Santa Clara, CA) prior to titer measurement by RP-UHPLC.
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bioRxiv - Molecular Biology 2021Quote: ... iPSC-derived kidney organoids were cut at a thickness of 4 μm using a cryo-microtome and mounted on FLEX IHC Microscope Slides (DAKO, Agilent Technologies, Amstelveen, Netherlands).
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bioRxiv - Genomics 2020Quote: ... and electroporated in 5 separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent Santa Clara, CA). After a 1 hour outgrowth in LB at 37C ...
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bioRxiv - Biophysics 2021Quote: ... incubated for 16 h at 4 °C and purified by HPLC on a Zorbax C3 column with dimensions of 21 × 150 mm (Agilent, Santa Clara, CA) using a gradient of 20-90 % acetonitrile in water with 0.1 % trifluoroacetic acid (TFA ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards the sections were incubated in a humidified chamber (overnight 4°C) in a peroxidase conjugated immunoglobulin against UEA (DAKO, P289; 1:50). Finally ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Bioengineering 2022Quote: ... eye cups were post-fixed for 10 min in 4 % FA-PBS at RT following by flat-mounting with fluorescent mounting medium (Dako, Carpinteria, CA, USA). Fluorescence images were acquired with Nikon Eclipse Ti-E microscope (Nikon ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated overnight at 4°C with the respective primary antibodies: (i) polyclonal rabbit anti-GFAP (1:2000; Dako, Cat. No. Z0334); (ii ...
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bioRxiv - Bioengineering 2024Quote: ... The solvent was then diluted with water (DMSO:water = 1:4) and subjected to purification through preparative reverse-phase HPLC chromatography (Agilent 1260 Infinity II HPLC). The chromatographic separation was carried out on an Agilent Prep-C18 column (250 mm × 21.2 mm × 10 μm ...
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bioRxiv - Bioengineering 2024Quote: ... The solvent was then diluted with water (DMSO:water = 1:4) and subjected to purification through preparative reverse-phase HPLC chromatography (Agilent 1260 Infinity II HPLC). The chromatographic separation was carried out on an Agilent Prep-C18 column (250 mm × 21.2 mm × 10 μm ...
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bioRxiv - Bioengineering 2024Quote: ... The solvent was then diluted with water (DMSO:water = 1:4) and subjected to purification through preparative reverse-phase HPLC chromatography (Agilent 1260 Infinity II HPLC). The chromatographic separation was carried out on an Agilent Prep-C18 column (250 mm × 21.2 mm × 10 μm ...
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bioRxiv - Molecular Biology 2024Quote: ... BRD2-BD mutant (N156A/N429A) and BRD3-BD mutant (N116A/N391A) were made using QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies 200517-4). The BRD4-BE mutant was modified from BRD4-BD to harbor E651A/E653A point mutations.
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bioRxiv - Cell Biology 2024Quote: ... Gene expression data for the GPR87 gene was extracted from the publicly available Lung Genomics Research Consortium (LGRC) data at the probe-level from the Agilent-014850 Whole Human Genome Microarray 4×44K G4112F (Agilent, Santa Clara, CA), as previously described25 ...
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bioRxiv - Microbiology 2021Quote: ... Tissue sections were colored via DAB-staining (3, 3-diaminobenzidine; Dako, K3468).
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bioRxiv - Microbiology 2022Quote: ... DirectDrive2 spectrometer operating at a 1H frequency of 600 MHz and using a 3.2-mm T3 HXY Magic Angle Spinning (MAS) probe (Agilent Technologies, Santa Clara, CA). These data were acquired on 34.1 and 39.7 mg ...
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bioRxiv - Biophysics 2020Quote: ... instrument operating at a 1H frequency of 600 MHz and equipped with a 3.2-mm T3 HXY MAS probe (Agilent Technologies, Santa Clara, CA). All data were acquired on ~12-18 mg of lyophilized melanin ghost or whole cell samples using a Magic Angle Spinning (MAS ...
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bioRxiv - Microbiology 2022Quote: ... instrument operating at a 1H frequency of 600 MHz and equipped with a 1.6-mm T3 HXY fastMAS probe (Agilent Technologies, Santa Clara, CA). The data were acquired on 6-8 mg of lyophilized cell material using a MAS rate of 15.00 ± 0.02 kHz at a spectrometer-set temperature of 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... spectrometer operating at a 1H frequency of 600 MHz and equipped with a 1.6-mm T3 HXY fastMAS probe (Agilent Technologies, Santa Clara, CA); all measurements were carried out using a magic-angle spinning (MAS ...
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bioRxiv - Developmental Biology 2024Quote: Perch at hatch were incubated at 25°C for 1h before the Seahorse XF Cell Mito Stress bioassay (Agilent, Santa Clara, United States). The Seahorse XF Calibrant fluid was used to hydrate the sensor plate for 24 h at 25°C before the assay ...
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bioRxiv - Cancer Biology 2022Quote: ... 3’-Diaminobenzidine (DAB, Agilent) and then counterstained with haematoxylin (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... followed by overnight incubation at 4 °C with α-α-SMA (1:400, mouse monoclonal, clone 1 A4, DAKO Agilent Technologies, Santa Clara, USA) or α-CD68 (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by overnight incubation at 4 °C with α-α-SMA (1:400, mouse monoclonal, clone 1 A4, DAKO Agilent Technologies, Santa Clara, USA) or α-CD68 (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
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bioRxiv - Cell Biology 2020Quote: ... Germany) followed by overnight incubation at 4 °C with specific primary antibodies: polyclonal rabbit anti-human AAT (1:800) (DAKO A/S, Glostrup, Denmark), mouse monoclonal anti-AAT polymer antibody (clone 2C1 ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Bioengineering 2022Quote: ... Cell nuclei were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 5 min before being mounted using DAKO mounting medium (Agilent, catalog no. S302380–2). Imaging of the histological samples was performed on the Microscope Axio Imager.A2 (Carl Zeiss Microscopy ...