Labshake search
Citations for Agilent :
3351 - 3400 of 3445 citations for 6 Bromo 3 4 dihydro 2H 1 benzothiin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... Cambridge, MA), CD68 (LV n=25, IVS n= 26, RV n=22) for macrophages (1:800, clone KP1, Dako/Agilent, Santa Clara, CA), and CD163 (LV n=26 ...
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bioRxiv - Pathology 2020Quote: ... followed by repeating all steps from antigen retrieval through TSA using an antibody against CK7 (1:200, mouse; Dako cat. no. MS-1352) and the FITC fluorophore (Perkin Elmer cat ...
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bioRxiv - Synthetic Biology 2020Quote: ... After hybridisation microarray slides were washed with Gene Expression Wash Buffer 1 and Gene Expression Wash Buffer 2 (Agilent, Cat No. 5188-5327) with added Triton X-102 (0.005% final conc. ...
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bioRxiv - Plant Biology 2019Quote: ... and gene-specific primers (Supplementary Table 1) to a final volume of 20 μl in clear 96-well plates with clear plastic lids (Agilent Technologies, Waldbronn, Germany). QRT-PCR assays were performed using an AriaMx Real-Time PCR system (G8830A instrument ...
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bioRxiv - Physiology 2021Quote: ... Antibodies bound to PCPE-1 were then detected by incubation with a HRP-conjugated rabbit anti-goat antibody (P0449; Dako; 50 ng/ml). After washing ...
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bioRxiv - Systems Biology 2019Quote: ... fluorescent chemistry and 1 ng was used to obtain RNA Integrity Number (RIN) using the Bioanalyzer RNA 6000 Pico kit (Agilent Technologies, 5067-1513). Lowest RIN was 9.5 ...
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bioRxiv - Microbiology 2019Quote: ... The reaction products for the total RNA of the human cell line KMM-1 were analysed by microfluidics-based automated electrophoresis with the Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: The pooled library was diluted to ~1 ng/uL and run on a Bioanalyzer High Sensitivity DNA chip (Agilent, Santa Clara, CA, USA) to check library purity and to estimate the expected ratio of host to microbial amplicons in the sample.
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bioRxiv - Immunology 2020Quote: The OCR and ECAR of THP-1 macrophages were determined using the Seahorse XFe24 Extracellular Flux Analyzer (Agilent Technologies, Santa Clara, CA, USA). Cells were seeded at 8 × 104 per well ...
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bioRxiv - Genomics 2020Quote: ... to enrich for fragments between 300-700 bp and 1 ul was used for quantitation and size analysis on an Agilent Bioanalyzer High Sensitivity D1000 chip (Agilent cat. 5067-5584). ATAC-seq libraries were subjected to 50 bp single-end sequencing on an Illumina Hi-Seq 4000 instrument ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated 1 hour at room temperature (RT) with EnVision HRP system anti-mouse (K400111-2, Agilent Technologies, Santa Clara, CA, USA) in blocking solution ...
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bioRxiv - Immunology 2022Quote: ... Quality control of the libraries to ensure no adapter dimers were present was carried out by examining 1ul of a 1:5 dilution on a High Sensitivity DNA chip (Agilent Technologies: 5067-4626) on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Pathology 2022Quote: ... and a monoclonal antibody directed against the alpha isoform of smooth muscle actin at a working dilution of 1/100 (a-SMA, clone 1A4, n M0851; Dako, Denmark A/S). Alligator skeletal muscle was used as positive control for anti-desmin antibody (Supplemental figure 3A) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight on a shaker at room temperature in fresh blocking solution with polyclonal rabbit anti-GFAP (1:1000, Dako, Santa Clara, CA). The following day tissue was washed in PBST and incubated for 2.5h at room temperature with Alexa Fluor 488 donkey anti-rabbit secondary (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... OCI-LY-1 cells (5 × 105 cells/ml) were pretreated for 1 hour in Seahorse Seahorse XF base medium supplemented with glutamine (103334-100, Agilent Technologies, Heverlee, Belgium) in a CO2-free incubator ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated 1 hour at room temperature (RT) with EnVision HRP system anti-mouse (K400111-2, Agilent Technologies, Santa Clara, CA, USA) in blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated at 37°C in a non-CO2 incubator for 1 hour and subsequently loaded into the Seahorse XFe96 Analyzer (Agilent Technologies, Seahorse Bioscience) when prompted ...
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bioRxiv - Microbiology 2023Quote: ... The quality of the DNA was checked on a Nanodrop to ensure that the OD600 260/230 and 260/280 ratios were >1.8 and the DNA length distribution was checked using a Femto Pulse (Agilent Technologies; Supplementary Figure 1).
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bioRxiv - Plant Biology 2023Quote: ... Peptides were dissolved in loading solvent A (0.1% TFA in water/ACN (98:2, v/v)) and desalted on a reversed-phase (RP) C18 OMIX tip (Agilent, Santa Clara, USA). The tip was first washed 3 times with 100 μl pre-wash buffer (0.1% TFA in water/ ACN (20:80 ...
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bioRxiv - Microbiology 2023Quote: ... tissues were incubated with proteinase K diluted 1:10 in 1X TBS (Fisher Scientific, Cat. #BP24711; and PNA ISH kit, Agilent Dako, Cat. #K5201) for 10 min at RT in a humidity chamber ...
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bioRxiv - Genomics 2023Quote: ... 0.9 μl of ‘11.1x buffer’ [21] and 0.06 μl of a 20:1 mix of PCRBIO Taq Polymerase (PCRBiosystems; 5U/μl): Pfu DNA polymerase (Agilent Technologies; 2.5 U/μl). PCR cycling conditions for the amplification of cat mitogenome segments generally consisted of an initial denaturation at 96°C for 1 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µL of individual polyribosomal RNAs were loaded on a PicoChip and run on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), according to the supplied protocol ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Extracts were then evaporated to a volume of approximately 15 μL of which 1 μL was analyzed using a 6890 gas chromatograph (GC) coupled to a 5975 mass selective detector (MS) (Agilent Technologies, Waldbronn, Germany). The GC was equipped with a DB-5 capillary column (0.25 mm ID × 30 m ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were incubated for 1 hour with horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit antibodies (Agilent Technologies, Santa Clara, CA, USA), washed with PBS 0.05% Tween ...
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bioRxiv - Plant Biology 2024Quote: ... The peptide-containing supernatant was subjected to solid phase extraction using SampliQ C18 cartridges (1 ml, 100 mg, 5982-1111, Agilent, Santa Clara, USA). Prior to the extraction the cartridge was washed successively with 1 ml 0.1% TFA,0.1% TFA in 80% ACN ...
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bioRxiv - Immunology 2024Quote: ... HER2 CAR-T cells or mock CAR-T cells from the same donor were added at an effector:target cell ratio of 10:1 and measured in a real-time cell analysis assay using the xCELLigence SP system (Agilent, Santa Clara, CA). An electrical current was applied to each co-culture ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibody solution was then washed out 3x with TBS-T and TBS-T + 5% NFDM + 1:2000 Dako goat anti-rabbit or anti-mouse HRP-conjugated immunoglobulins (Agilent, Santa Clara, CA, USA) was added ...
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bioRxiv - Cancer Biology 2020Quote: DNA from cell lines was isolated as previously described 12 and used to analyze copy number variations by the Array-CGH SurePrint G3 Human CGH Microarray 1×1M (Agilent, Santa Clara, California, USA). Array experiments were performed as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... and with secondary goat anti-rabbit (for polyclonal Ab) or rabbit anti-mouse (for monoclonal Ab) IgG HRP-conjugated Ab (1:2000, Dako A/S, Glostrup, Denmark). Signals were visualized by Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
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bioRxiv - Microbiology 2019Quote: ... Desalted tryptic peptides corresponding to 1 μg of protein were loaded for LC-MS/MS analysis with an Agilent 1100 Capillary LC system (Agilent Technologies, San Jose, CA) and run on a Q Exactive mass spectrometer (ThermoFisher Scientific Inc.) ...
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bioRxiv - Biochemistry 2020Quote: ... The TIMS elution voltage was calibrated linearly to obtain reduced ion mobility coefficients (1/K0) using three selected ions (m/z 622, 922, and 1222) of the ESI-L Tuning Mix (Agilent, Santa Clara, CA, USA).
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bioRxiv - Biochemistry 2020Quote: ... Tyrosine fluorescence measurements were carried out in triplicate at 20 °C using a quartz cuvette (Hellma Asia Pte. Ltd. Singapore) at 1 cm path length on a Varian Cary Eclipse (Agilent Technologies Singapore Pte. Ltd) fluorescence spectrophotometer with excitation and emission wavelengths of 275 nm and 305 nm respectively ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 45 minutes at room temperature with the anti-SARS spike glycoprotein antibody 3A2 (rabbit, Abcam ab272420 1:100 diluted in Dako REAL antibody diluent #S2022), which has been validated in previous publications20,41 ...
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bioRxiv - Developmental Biology 2022Quote: ... Mutations were generated within the miR-1 seed sequences using the QuikChange Lightning or QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, California) to disrupt miR-1’s binding and regulatory function (Gregory et al. ...
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bioRxiv - Cell Biology 2022Quote: ... T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM; 103575-100) supplemented with 4mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... Phe322 was substituted for the Tyr322 residue of WT and G307S DNAM-1 by using QuikChange II Site-Directed Mutagenesis Kits (Agilent technologies, Palo Alto, CA) with primers (5′-GAGAGGATATTTTTGTCAACTATCCAACC-3′ and 5′-GGTTGGATAGTTGACAAAAATATCCTCTC-3′) ...
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bioRxiv - Genomics 2019Quote: ... We also ensured the quality of library pools by running 1 µL on a Bioanalyzer High Sensitivity chip (Agilent Technologies, Santa Clara, CA, USA). We combined the iTru and iNext E ...
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bioRxiv - Pathology 2020Quote: ... Abcam, Cambridge, MA), CD68 (LV n=25, IVS n= 26, RV n=22) for macrophages (1:800, clone KP1, Dako/Agilent, Santa Clara, CA), and CD163 (LV n=26 ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein samples were separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblot analysis. Membranes were incubated with primary antibodies (Suppl. Table 1) overnight and probed with respective secondary antibodies (Dako-Agilent, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... 1 µl of the sample was injected into an Agilent 7890B equipped with an Agilent 7000D mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). The injector was set to 170°C and heated to 350°C with 200°C/min and hold for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed with 1X phosphate-buffered saline (PBS) and incubated in a humidified chamber for 1 hour at room temperature with protein-blocking solution (Agilent Dako, Santa Clara, ON, Canada) to block nonspecific sites ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with 50 µl/well of HRP-conjugated rabbit anti-mouse total IgG (1:1,000 in ELISA dilution buffer; P0260, Dako Agilent, Santa Clara, CA, USA), goat-anti-mouse IgG1 (1:8,000 in ELISA dilution buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and fluorescence intensity was measured at 340/440 nm over 1 hr at 37 °C using a BioTek Synergy HTX (Agilent, Santa Clara, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... The fluorogenic substrate was added to each well of a 96-well plate and the fluorescence intensity was measured at 340/440 nm over a 1 hr period at 37 °C using a Bio-Tek Synergy HTX (Agilent, Santa Clara, CA, USA) plate reader.
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bioRxiv - Plant Biology 2023Quote: ... Peptides were desalted using C18 solid phase extraction columns (Bond Elut SPEC C18, 96 round-well plate, 15 mg, 1 mL, Agilent Technologies, Santa Clara, USA) and a water-jet (vacuum ...
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bioRxiv - Bioengineering 2024Quote: ... GBM cells within hydrogels were seeded at a density of 1 × 105 cells/well in 96-well spheroid microplates (Agilent, catalog number 102959-100). In the Mito Stress Test ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections on slides were baked at 60°C for 1 h and then deparaffinized and retrieved on Dako Omnis fully automated IHC platform (Agilent, Santa Clara, CA, USA) using EnVision FLEX target retrieval solution ...
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bioRxiv - Microbiology 2023Quote: ... One microliter of each methoximated and trimethylsilylated sample was injected by 2:1 split injection by an Agilent Technologies 7693 autosampler (Agilent Technologies, Santa Clara; California) into an Agilent 7890A gas chromatograph equipped with a fused silica capillary column (0.25 mm × 30 m ...
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bioRxiv - Microbiology 2023Quote: ... tissues were incubated with proteinase K diluted 1:10 in 1X TBS (Fisher Scientific, Cat. #BP24711; and PNA ISH kit, Agilent Dako, Cat. #K5201) for 10 min at RT in a humidity chamber ...