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Citations for Agilent :
3101 - 3150 of 3445 citations for 6 Bromo 3 4 dihydro 2H 1 benzothiin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and Seahorse XF Cell Mito Stress Test Kit (catalog number 103015-100, contains 1 each of oligomycin, FCCP, and rotenone/antimycin A.) were all purchased from Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent).
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were incubated overnight with the following primary antibodies: rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:6,000, DAKO, UK), rabbit polyclonal anti-allograft inflammatory factor 1 (IBA1 ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal goat anti-rabbit secondary antibody IgG/HRP (P044801-2; Agilent Technologies/Dako ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal rabbit anti-mouse secondary antibody IgG/HRP (P0260; Agilent Technologies/Dako) diluted 1:2000 in 3% BSA.
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bioRxiv - Genetics 2023Quote: ... the Prab-3p::hpk-1 plasmid was used and mutations were performed with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All the assembled plasmids were validated by sequencing prior to microinjection ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated for 30 minutes with the rabbit anti-human HES1 antibody diluted 1:100 in a low background diluent (Dako). A linker rabbit was used to amplify the signal ...
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bioRxiv - Physiology 2023Quote: ... Membranes were then washed in TBST and incubated with the secondary HRP-conjugated antibodies: goat anti-rabbit (1∶1000, P0448, DAKO) or polyclonal anti-mouse (1∶2000 ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with either horse radish peroxidase (HRP)-labeled anti-rabbit IgG (1:20000, Agilent Dako, Poland) or HRP-labeled anti-mouse IgG (1:20000 ...
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bioRxiv - Cancer Biology 2023Quote: ... ~100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with either horse radish peroxidase (HRP)-labeled anti-rabbit IgG (1:20000, Agilent Dako, Poland) or HRP-labeled anti-mouse IgG (1:20000 ...
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1 ERG (GenBank accession. NM_182918) Myc-tagged construct was mutated using the QuickChange Lightning Multi SiteDirected Mutagenesis kit (Agilent: #210513). All primers were designed using the QuickChange Primer Design Program (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with 1x PBST for 5 min and incubated with secondary antibodies anti-mouse-HRP (1:10,000) (Agilent Dako, #P0447) and anti-rabbit-HRP (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality was determined by running 1 μL of the sample on a BioAnalyzer RNA Nano chip (Agilent, No. 5067-1511) for RIN ...
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bioRxiv - Genomics 2023Quote: ... The percentage of the genome fragmented to sizes between 1 – 4 kb was then empirically measured by fragmenting 1 µg of genomic DNA followed by quantification on a Genomic DNA ScreenTape assay (Agilent). Hpy166II fragments 41% of the genome to within the target size range ...
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bioRxiv - Plant Biology 2023Quote: CDS sequences from PhDEF and PhGLO1 were amplified from Petunia x hybrida R27 inflorescence cDNAs with primers MLY2382/MLY2383 and MLY2384/2385 respectively (Supplementary Table 1) and cloned into the in vitro translation vector pSPUTK (Stratagene) by NcoI/XbaI restriction ...
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue samples were incubated for 60 min at room temperature with primary antibodies (Monoclonal Mouse Anti-Human Ki67, clone MIB-1, Dako North America Inc. ...
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bioRxiv - Systems Biology 2023Quote: ... we calibrated the TIMS elution voltages by known 1/K0 values from at least two out of three ions from Agilent ESI LC/MS tuning mix (m/z ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-incubated for 1 h and treated in Agilent Seahorse XF Base Medium (Agilent Technologies, cat#103334-100), which is optimized for extracellular flux assays and contains thiamine at 8.2 μM concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed once with DMEM Assay Medium (XF DMEM [Agilent] supplemented with XF glucose [10 mM, Agilent], XF glutamine [2 mM, Agilent] and XF pyruvate [1 mM, Agilent]), then left in DMEM Assay Medium and placed in a non-CO2 incubator for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... 10 µg/mL of either mouse anti-human LAIR-1 (clone Dx26; purified in house) or mouse isotype control IgG1 (Dako) diluted in PBS+1% BSA buffer were incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient-matched primary tumor tissues and PDTOs were immunostained for diagnostic NSCLC markers with antibodies against cytokeratin (CK)7 (mouse, M7018, 1:100; Dako), CK5/6 (mouse ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed in PBS and incubated in DAPI (1:500) for 5 min before mounting with the immunofluorescence mounting media (DAKO).
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bioRxiv - Plant Biology 2023Quote: ... RNA integrity was confirmed by agarose (1% w/v) gel electrophoresis and by microfluidic electrophoresis using a Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... For staining of macrophages and platelets the aortic tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting peptide mixture was acidified by addition of 1% trifluoroacetic acid (TFA) and desalted on a reversed phase (RP) C18 OMIX tip (Agilent). The tip was first washed 3 times with 200 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
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bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
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bioRxiv - Cancer Biology 2024Quote: ... and anti-GGCT (16257-1-AP; proteintech) antibodies were used for immunodetection on rehydrated sections pretreated with Target Retrieval Solution (S1699; DAKO). The Envision™+ system (HRP/DAB ...
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bioRxiv - Immunology 2024Quote: ... after which live cell imaging analysis was carried out for 30 minutes at a rate of 1 frame per 30 or 40 seconds using BioTek LION Heart XL (Agilent) or TomoStudio (Tomocube ...
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bioRxiv - Cancer Biology 2024Quote: ... These tumor sections were stained at the Histology core (Henry Ford Health) according to the manufacturer’s protocols (Ki-67 IHC MIB-1, Dako Omnis). Proliferating cells were immunostained with FLEX monoclonal mouse anti-human Ki-67 (Catalog No ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: The following secondary antibodies were used in this study: rabbit anti-mouse immunoglobulins/HRP (Agilent Cat# P0260, RRID:AB_2636929, 1/10000), goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... The library profiles were checked using High Sensitivity NGS Fragment Analysis kit (1-6000bp) (Agilent Technologies, reference DNF-474-1000) on the 5300 Fragment Analyzer System ...
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bioRxiv - Cell Biology 2024Quote: ... Qualitative and quantitative measurement of cDNA was conducted with the High Sensitivity NGS Fragment 1-6000bp kit on a 48-channel Fragment Analyzer (Agilent) and 1x dsDNA kit on the Qubit 4 Fluorometer (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... One aliquot was solubilized with 1% SDS to determine protein content and the other aliquot was mixed with 1 mL 30% HNO3 and subjected to ICP/MS 8900 (Agilent). Data was normalized by the corresponding protein concentration.
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bioRxiv - Plant Biology 2024Quote: ... A splitless injection volume of 1 μL was injected onto an HP-5 column with 5% phenyl methylpolysiloxane stationary phase (Agilent) with dimensions of 30 m x 0.25 mm x 0.25 μm ...
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bioRxiv - Biochemistry 2024Quote: The HAdV5HVR7 vectors (“1st generation” vectors) were obtained by cloning the gene of interest into the pAdEasy-1 plasmid system from Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... 2 mM Seahorse XF L-glutamine solution and 1 mM Seahorse XF pyruvate solution (103577-100, 103579-100 and 103578-100, all from Agilent) and cells were kept in a CO2 free incubator for 45 min prior to starting the Cell Mito Stress Test ...
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bioRxiv - Immunology 2024Quote: ... Sections were blocked with 5% horse serum in 1% BSA solution and incubated with the anti-CD68 primary antibody (KP1, Dako) for 60 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... which was amplified in 15 PCR cycles using Herculase II Fusion DNA polymerase in a 100-μl reaction mixtures (1x Herculase II reaction buffer, 1 mM dNTPs, 200 pM Agilent library ...
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bioRxiv - Neuroscience 2024Quote: Cells were counted and plated at 1 × 105 (mouse astrocytes or neurons) cells per well in a Seahorse XF24 Cell Culture Microplate for all experiments (Agilent). Cells were then treated with indicated inhibitors or agonists in each experiment for 20 h ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then incubated in a 0% CO2 chamber at 37 °C for 1 h before being placed into a Seahorse XFe24 Analyzer (Agilent). For OCR and ECAR mitostress test experiments ...
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bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were subsequently separated by Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Bands were quantified and PSI was calculated as follows ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue was hybridized with an antibody against CD138/Syndecan-1 (clone MI15, mouse, #M7228, Agilent, Santa Clara, CA, USA), following standard procedures by automated processing using the Tecan Evo Freedom stainer and the Dako Advance system according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
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bioRxiv - Bioengineering 2021Quote: ... 10-20 µl injection volume depending on protein concentration) were injected on a Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) at a flow rate of 100 µl/min using solvent A (0.1% formic acid and 0.05% trifluoroacetic acid in water ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to synthesize cDNA from 1 ug RNA and Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) was used for the Quantitative RT-PCR reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1 hour incubation with secondary antibodies diluted in 2.5% milk in TBST (1:5000, goat anti-rabbit HRP, or goat anti-mouse HRP, DAKO, #P0448 & #P0447). Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo ...