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Citations for Agilent :
3301 - 3350 of 3795 citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated for 1hr at room temperature with polyclonal HRP-conjugated goat anti-rabbit (1:5000; Dako, Denmark) or anti-mouse (1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were then incubated with anti-PD-L2 (CST #82723) diluted 1:25 in EnVision FLEX antibody diluent (Dako) over night at 4º C ...
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bioRxiv - Biochemistry 2022Quote: ... The column exit was connected to the ESI source of the mass spectrometer using PEEK tubing (0.005” inner diameter × 1/16” outer diameter, Agilent). Mass spectra were acquired in the positive ion mode over the range m/z = 350 to 1800 using the Orbitrap mass analyzer ...
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bioRxiv - Cell Biology 2022Quote: ... the cell nuclei were stained with a 1:2000 dilution of SPY-DNA dye (Spirochrome) in antibody diluent (Dako). SPY-DNA 505 ...
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bioRxiv - Immunology 2022Quote: ... and a 1:10 dilution of the resulting cDNA was run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess cDNA quality and yield ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... then reconstituted in 1 mL of 0.1 % trifluoroacetic acid and desalted using an Agilent Macroporous Reversed-Phase C18 column (4.6 × 50 mm mRP-C18, Agilent) on a 1260 Infinity HPLC system (Agilent).
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bioRxiv - Neuroscience 2022Quote: ... The slides were washed with 0.1 M Sorensen’s phosphate buffer and the staining was developed with a 1:50 solution of DAB chromogen (K3468, Dako) and 0.005% hydrogen peroxide in Sorensen’s phosphate buffer for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... The staining was developed using a 1:50 dilution of 3,3’-diaminobenzidine (DAB) chromogen in DAB+ substrate buffer (GV825, Dako) for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... CFSs were diluted in SDW (ratio 1/20) and injected into a high-performance liquid chromatography (HPLC) system (Agilent 1100 Series 203 HPLC System ...
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bioRxiv - Cell Biology 2022Quote: The following secondary antibodies were used for western blot experiments: peroxidase-conjugated goat anti-rabbit (P448, DAKO, 1:1000) and goat anti-mouse (P0447 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and an Agilent 7693A autosampler fitted with a 100 μL gastight syringe was used to inject headspace gas (50 μL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 μm film; Agilent). Data analysis was performed using Agilent MassHunter WorkStation Quantitative Analysis ...
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bioRxiv - Synthetic Biology 2024Quote: ... and an Agilent 7693A autosampler fitted with a 100 μL gastight syringe was used to inject headspace gas (50 μL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 μm film; Agilent), and (iii ...
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bioRxiv - Physiology 2024Quote: ... live cells stained with Hoechst 33342 (1:2,000 dilution) (ThermoScientific, Cat#62249) were enumerated using Cytation 5 plate reader (BioTek Instruments - Agilent). Values obtained from the Mito-Stress test were normalized per 1,000 cells.
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bioRxiv - Genomics 2024Quote: ... The quality of nucleic acids was assessed by agarose gel electrophoresis (1% for RNA, 0.7% for DNA) and on-chip electrophoresis (Bioanalyzer 2100, Agilent).
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... GC-FID was performed using an Agilent 7890 gas chromatograph equipped with a DB-WAX column (15 m x 0.53 mm, 1 µm; Agilent) and flame ionization detection ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using the 1-Step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies) and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Rehydrated 5-μm sections were stained with primary antibodies against insulin (dilution 1:1000; Dako, Santa Clara, CA, USA), somatostatin (dilution 1:300 ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Microbiology 2023Quote: ... RNA integrity was assessed with 1% (m/v) agarose gel and Agilent 2100 Bioanalyzer System (Agilent Technologies, 5067-4626). For antibiotic treatment conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... the beads were transferred into a 1-mL PP filtration microplate (Agilent, 7 µm frit, cat. no. 202501-100) and washed 3x with 200 µL 1x PBS and 2x 200 µL ultrapure water ...
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bioRxiv - Biophysics 2023Quote: Drp1 isoform 1 (Genbank accession number AB006965) was PCR amplified with Pfu Turbo DNA polymerase (Stratagene, La Jolla, CA) as an NdeI/XhoI fragment ...
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bioRxiv - Biochemistry 2023Quote: ... and m6A(1-100 mM, MCE HY-N0086) were analyzed on RapidFire 300 high-throughput SPE system (Agilent Technologies) interfaced with a Sciex 6500 (Sciex) ...
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bioRxiv - Biochemistry 2023Quote: ... RNA quality was measured by 1% agarose gel electrophoresis (Figure S3A) and by High Sensitivity (HS) DNA Assay (Agilent) (Figure S3B).
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Synthetic Biology 2023Quote: ... An Agilent 7693A autosampler fitted with a 100 µL gastight syringe was used to inject headspace gas (50 µL) into a DB-VRX capillary column (20 m, 0.18 mm ID, 1 µm film; Agilent). Experiments were performed using a 55:1 split ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then rinsed with PBS containing 1% FBS prior to imaging using a Cytation 5 instrument (Agilent BioTek). Mean fluorescence was calculated using Gen5 software (RRID:SCR_017317).
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Oxygen consumption rates (OCR ...
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bioRxiv - Cell Biology 2024Quote: ... and qualitatively assessed with the High Sensitivity NGS Fragment 1-6000bp Kit on a 96-channel Fragment Analyzer (Agilent). Final libraries were normalized ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were also assessed for size distribution and adapter dimer presence (<0.5%) by the High Sensitivity NGS Fragment 1-6000bp kit on a 96-channel Fragment Analyzer (Agilent). All sequencing libraries were then normalized on the MicroLab STAR (Hamilton) ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added with the estimation that OD600 of 1 is approximately equal to 8×108 CFU/ml (Agilent biocalculator). The MOI of 0.01 was administered to E ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed in PBS-T (0.05% Tween- 20 in PBS) and PBS before incubation with either 1:20,000 dilution of HRP-conjugated rabbit anti-human IgG (Dako) or 1:10,0000 dilution of HRP-conjugated goat anti-mouse IgG (Seracare ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were probed with appropriate primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1/5000 (Dako Agilent). Proteins were detected with ECL Western Blotting Substrate (Promega ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were probed with appropriate primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1/5000 (Dako Agilent). Proteins were detected with ECL Western Blotting Substrate (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... We characterized a 1/10 dilution of both spikes using the TapeStation RNA HS kit (Agilent Technologies, CA, USA) and compared the true size distribution to the theoretical one (as calculated using the manufacturer’s molarity and sequence files).
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies used for detection were PHF1 (pSer396/404) tau (1:1000, Peter Davies, Cat# PHF1; RRID: AB_2315150) and anti-tau (Dako, 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and the chambers were left to dry before imaging with Axio Observer.Z1/7 (Zeiss) or the BioTek Cytation 1 (Agilent).
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bioRxiv - Bioengineering 2024Quote: ... The reaction was initiated by adding purified RnKICD wild-type or mutant enzyme (0.1 mg mL-1) to the reaction mixture into 20 mL GC-MS headspace vials (5188-2753, Agilent) sealed with PTFE-lined screw caps (5188-2759 ...
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bioRxiv - Biochemistry 2024Quote: ... The cHMM or cS1 cDNA was cloned into the pShuttle-IRES-hrGFP-1 vector (Agilent Tech., Santa Clara, CA) and an Ad-Myo-Flag virus was prepared and amplified for expression of cHMM/cS1 protein in C2C12 cells ...
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bioRxiv - Plant Biology 2020Quote: ... The digest was filtered (0.22 μm) and filtered samples (1-2 μg) were loaded onto a C18 high capacity nano LC chip (Agilent Technologies) using a 1200 series capillary and eluted directly into a 6550 series quadrupole time-of-flight mass spectrometer (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected into the GC (Sigma-Aldrich 5973 Network mass selective detector attached to an Agilent 6890 gas chromatograph (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... Two µl of samples were introduced in the splitless injection mode in DB-5 (60 m × 0.25 mm, 1 µm film thickness, Agilent, CA) using helium as a carrier gas ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... or 1 µM (OMM1) carbonyl cyanide 4-(trifluoromethoxy)-phenylhydrazone (FCCP) and 0.5 µM of a rotenone antimycin A mix (Agilent Technologies). Following the assay ...
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bioRxiv - Cancer Biology 2021Quote: ... The plate was incubated in a CO2-free 37°C incubator for 1 hour and a Mitochondrial Stress Test (Agilent) was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... S34F or Q157R were treated with doxycycline (1 μg/mL) for 48 hours before UV-crosslinking (400 mJ/cm2 UV Stratalinker 2400, STRATAGENE). RNA-protein complexes were immunoprecipitated with 12 μg anti-FLAG M2 antibody (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... ∼100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... and the cDNA hybridised to an 8 Chamber Agilent slide at 65°C for 16 hours before washing the slide with Oligo aCGH Wash Buffer 1 (Agilent) for 5 minutes at room temperature and Oligo aCGH Wash Buffer 2 (Agilent ...