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Citations for Agilent :
3101 - 3150 of 5728 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... coli ArcticExpress (DE3) cells (Agilent Technologies, Santa Clara, CA, USA) similar as previously described [5] ...
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bioRxiv - Immunology 2023Quote: ... T cells were plated in Seahorse XF DMEM Medium (Agilent) supplemented with 1% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was transformed into E.coli BL21(DE3) cells (StrataGene) and protein expression induced with 0.5 mM IPTG at 37°C for 4 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell numbers were measured using Synergy LX Multimode Reader (Agilent) and Cell Count Reagent SF Kit (Nacalai Tesque) ...
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bioRxiv - Cell Biology 2023Quote: Cells were blocked with serum free Protein Block reagent (Dako) at RT for 1hr and stained with primary antibodies diluted in Antibody Diluent (Dako ...
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bioRxiv - Cell Biology 2024Quote: Agilent seahorse XF96 cell culture microplate (Agilent technologies,101085-004) was first coated with 30 μL poly-D-lysine (P6407 ...
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bioRxiv - Neuroscience 2024Quote: ... before cells were mounted in DAKO fluorescent mounting medium (DAKO). Primary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... Assembled molecules were transformed into XL1-Blue competent cells (Agilent) and final expression plasmid was isolated using QIAGEN Plasmid Midi Kit (Qiagen ...
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bioRxiv - Biophysics 2024Quote: ... coli BL21-CodonPlus (DE3)-RIPL cells (Agilent, Catalog Number 230280). Cells were grown at 37 °C to an optical density at 600 nm (OD600 ...
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bioRxiv - Microbiology 2024Quote: ... coli BL21 (DE3) cells containing the RIL Camr plasmid (Stratagene). A comprehensive list of all the strains ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed twice with Agilent Seahorse XF medium (Agilent) supplemented with 1 mM pyruvate ...
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bioRxiv - Immunology 2024Quote: Seahorse XF Cell Mito Stress kits were purchased from Agilent Technologies ...
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bioRxiv - Physiology 2020Quote: ... Real-time PCR amplification with the 2 x precision master mix (Primer design, Southampton, UK) using the Stratagene Mx3000P real-time QPCR system (Agilent Technologies, Santa Clara, CA, USA). Each sample was tested in triplicate and compared to a housekeeping gene (Atp5B in osteoblasts and bone tissue ...
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bioRxiv - Molecular Biology 2020Quote: ... and aromatic 1H-13C-NOESY-HSQC experiments with a mixing time of 150 ms each were carried out at 25 °C on a 500 MHz Agilent DirectDrive 2 spectrometer (Agilent Technologies, Santa Clara, CA, USA) equipped with a room temperature probe ...
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bioRxiv - Immunology 2022Quote: ... then incubated in 5 μg/ml primary antibody in incubation buffer (anti-Mala s 1 mouse monoclonal IgG1 antibody (9G9) from Karolinska Institute (Zagari A et al, 1994, Schmidt et al, 1997) or mouse monoclonal IgG1 antibody (Agilent Dako X093101-2; Santa Clara, USA) as an isotype control for 90 min ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed again three times in TBS 1X and immunoreactions were revealed with DAB peroxidase substrate (K346811-2, Agilent Technologies, Santa Clara, CA, USA) was performed ...
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bioRxiv - Microbiology 2024Quote: ... Colorimetric conversion was terminated by addition of 5% SDS (50 uL per well) and absorbance was measured at 414 nm using a BioTek Epoch 2 Microplate Spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). The endpoint absorbance of each sample was determined on a plate-by-plate basis where the absorbances of all the blank wells were averaged and multiplied by three ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed again three times in TBS 1X and immunoreactions were revealed with DAB peroxidase substrate (K346811-2, Agilent Technologies, Santa Clara, CA, USA). Finally ...
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bioRxiv - Biophysics 2023Quote: The samples were shortly centrifuged after thawing and injected onto a cooled (2°C) HPLC System (Agilent Infinity 1260, Agilent Technologies, Santa Clara, CA, USA) which includes a home packed pepsin column (IDEX guard column with 60 μl Porozyme immobilized pepsin beads ...
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bioRxiv - Biochemistry 2023Quote: ... The fluorescence at 590 nm with excitation at 485 nm (F590) was measured with the BioTek Synergy 2 plate reader (Agilent Technologies, Santa Clara, CA, USA). The data were fitted using the Hill equation to estimate parameters from data which has the a priori unknown parameters ...
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bioRxiv - Cell Biology 2023Quote: 5 000 wild type and UbKEKS knockout HeLa cells were seeded and cultured as adherent cells in XCELLigence RTCA E-plates (Agilent E-plate VIEW 16, #300601140). Cells were incubated at 37°C under normal conditions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and the consumption of NADPH was followed at 30 °C and 340 nm (Δε=6.22 mM-1 cm-1) with a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Germany).
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bioRxiv - Evolutionary Biology 2020Quote: ... The reaction was started with different concentrations of 3-butenoic acid and the consumption of NADPH was followed at 30 °C and 340 nm (Δε=6.22mM-1 cm-1) with a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Germany).
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bioRxiv - Neuroscience 2020Quote: Mouse brains were analyzed as free-floating sections to localize immunoreactivity for GFAP (Z0334, Dako/Agilent; 1:10,000 primary, 1:10,000 secondary), Iba-1 (019-19741 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight at 4° C with primary antibodies (mouse-anti-Ataxin-3, 1:2500, clone 1H9, MAB5360, MerckMillipore, Darmstadt, Germany; rabbit-anti-Ubiquitin, 1:500, Z0458, Dako, Jena, Germany). After washing the membranes were incubated with secondary antibodies (peroxidase affinePure donkey anti-mouse IgG H+L ...
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bioRxiv - Cell Biology 2022Quote: ... IgG1a monoclonal antibody in PBS with 1% BSA followed by a bridge step labelling with a polyclonal Rabbit Anti-Mouse Immunoglobulins (Dako, 1:50) and protein A-gold 10 nm (UMC Utrecht ...
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bioRxiv - Immunology 2020Quote: ... followed by the incubation with horseradish peroxidase-labeled secondary antibody (1:10,000) for 1 h at room temperature (DAKO EnVision™+ System; Agilent Technologies (UK). Diaminobenzidine (DAB ...
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bioRxiv - Immunology 2020Quote: ... 1:4000, rat anti-mouse IgG3-biotin (Becton, Dickinson, Franklin Lakes, New Jersey) 1:2000 followed by streptavidin-HRP (Dako, Glostrup, Denmark) 1:1000 incubated at 37°C for 1h.
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated for 1 h at room temperature with rabbit anti-glial fibrillary acidic protein (GFAP, 1:300; Dako, Carpinteria, CA), rabbit anti-oligodendrocyte specific protein (OSP ...
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bioRxiv - Microbiology 2022Quote: ... primary antibody was produced in-house from HB 65 mouse anti-nucleoprotein and diluted 1:2500 followed by HRP-conjugated rabbit anti-mouse diluted 1:500 (Dako, Glostrup, Denmark). The titration was repeated on a different day and TCID50 titres were calculated using Reed and Muench (29).
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP, Dako, 1:2500), and washed 3 times 15 minutes in TBST again before chemiluminescence detection ...
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bioRxiv - Molecular Biology 2022Quote: ... Consecutive slides were incubated with anti-ATM (1:100) or anti-SMA (1:200) diluted in Dako REAL Antibody Diluent (Dako, Glostrup, Denmark). Slides were then treated with biotinylated secondary antibodies and target staining was performed with peroxidase-conjugated streptavidin and DAB chromogen (Dako REAL Detection System Peroxidase/DAB+ ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with secondary antibodies diluted in 0.5% WBR in TBST for 1 hour at room temperature (Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP, Agilent Dako #P0448, 1/2000), washed three times with TBST ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP, Dako, 1:2500), and washed 3 times 15 minutes in TBST again before chemiluminescence detection ...
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bioRxiv - Immunology 2023Quote: ... The final NGS libraries were obtained using SPRI beads at a 1:1 ratio and quantified with a 4200 TapeStation System (Agilent Technologies, G2991BA). Libraries with a single peak of appropriate length were subjected to NGS analysis using the Illumina Novaseq6000 platform.
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bioRxiv - Neuroscience 2023Quote: ... sympathetic ganglia and spinal cords was conducted directly on the slides using primary rabbit anti-HSV-1 (1:100; B0114, DakoCytomation, Carpinteria, CA) and chicken anti-GFP (1:400 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-CD44 (1:50, clone DF1485, Dako, M7082), rabbit anti-EGFR (prediluted ...
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bioRxiv - Cell Biology 2020Quote: ... or goat anti-mouse IgG/HRP (1:5000, Dako) for 1 h at room temperature before being washed ...
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bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase-conjugated anti-mouse (Dako, #P0447; 1:4,000) or anti-rabbit IgG (gamma-chain specific ...
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bioRxiv - Genetics 2021Quote: ... and mouse-anti-Ki67 (Agilent, M7248, dilution 1:100). Goat-anti-mouse-488 and Goat-anti-rabbit-568 were used as secondary antibodies and sections were counterstained with DAPI ...
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bioRxiv - Microbiology 2021Quote: ... then 1:1500 goat anti-mouse IgG-HRP (Dako); 1:1000 anti-HA (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... then 1:1500 goat anti-mouse IgG-HRP (Dako). Membranes were washed for 3 × 5 mins in TBST after each antibody step ...
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bioRxiv - Cell Biology 2022Quote: ... or goat anti-mouse (DAKO, cat# P0447; 1:4,000).
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bioRxiv - Neuroscience 2021Quote: ... or rabbit anti-GFAP (1:500, Dako, Glostrup, Denmark) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... or rabbit anti-S100 (Dako, Z033, 1:100, discontinued) (Supplementary Table 5) ...
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bioRxiv - Molecular Biology 2021Quote: ... Horseradish Peroxidase conjuguated anti-mouse IgG (Dako, 1/5000) and anti-rabbit IgG (Dako ...
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bioRxiv - Immunology 2022Quote: ... neutrophil elastase (clone NP57, Dako M0752, dilution 1:100), CD68 (KP1 ...
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bioRxiv - Immunology 2021Quote: ... using the Silencer Select Negative Control #1 (Agilent, ThermoFisher) as control ...
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bioRxiv - Cell Biology 2019Quote: ... or goat anti mouse-HRP (1:100, P0447, DAKO) (type II collagen ...
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bioRxiv - Neuroscience 2020Quote: ... and probed with antibodies to ApoE (1:300; Dako) and beta-tubulin (1:1000 ...