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Citations for Agilent :
3001 - 3050 of 5728 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Both HSV-tk and col3 used a secondary polymer-HRP anti-rabbit antibody incubation for 1 hr (1:200 Dako P0448). For col2 and endomucin staining the manufacturer’s instructions were followed (Vectastain Elite ABC kit ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunostaining was performed as described previously(45) with the following antibodies: guinea pig α-insulin (1:2000, Millipore/1:800, DAKO), mouse α-glucagon (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK; 1:800) in Antibody Diluent (S0809, Agilent DAKO). Following removal of excess primary antibody with wash buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were washed for 3 x 10 min in 1 x Animal-Free Blocker and incubated for 1 h at room temperature in HRP-conjugated rabbit anti-goat secondary antibody (1:1000, Dako, USA) for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM Vanadyl-ribonucleoside complex NEB S1402S) mixed with 1:3000 dilution of probes and 1:900 dilution of guinea pig anti-Insulin antibody (Dako, A0564). Hybridization mix was incubated with tissues for overnight in a 30°C incubator ...
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bioRxiv - Immunology 2022Quote: Tissue sections were then incubated with anti-PD1 (1:300, 3G8) and anti-PDL1 (1:100, 1F8) in antibody diluent (Dako, S0809) for 60 min (26) ...
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bioRxiv - Immunology 2020Quote: ... 1 μM fluoro-carbonyl cyanide phenylhydrazone (FCCP) and 100 nM rotenone + 1 μM antimycin A (all from Agilent, Santa Clara, California) using a 96 well XF Extracellular Flux Analyzer (EFA ...
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bioRxiv - Neuroscience 2019Quote: ... The slides were incubated overnight in primary antibody (ATRX: 1:200, H-300 Santa Cruz Biotechnology Inc; GFAP: 1:200, Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... then primary antibody (anti-SOX9 at 1/200 with 0.1% TritonX-100, ABCAM, Cat # ab185966; anti-KRT19 at 1/100, DAKO Cat # M0888) diluted in diluent (DAKO ...
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bioRxiv - Cell Biology 2020Quote: ... and the following HRP conjugated secondary antibodies: swine anti-rabbit (1:3000 for embryo levels, 1:10000 for in vitro assays; Dako, P0399) and sheep ECL anti-mouse (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated antibodies were used as secondary antibodies (Swine anti-rabbit 1:3000, Goat anti-mouse 1:5000, Dako, Glostrup, Denmark). Membranes were developed with Clarity Western ECL Substrate 1:1 (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... dried metabolites were resuspended in 50 μL of a 1:1 DMSO to water solution and analyzed by LC-MS using a 1290 Infinity II liquid chromatography (Agilent Technologies) with a thermal autosampler set at 4°C ...
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bioRxiv - Pathology 2024Quote: ... sections were incubated overnight at 4 ⁰C with a cocktail of the primary antibodies αSyn (clone KM51 1:500, Monosan Xtra, The Netherlands) and glial fibrillary acidic protein (GFAP, Z0334, 1:4000, DAKO, Denmark). After rinsing in PBS ...
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bioRxiv - Immunology 2024Quote: ... The membrane was washed three times in block buffer followed by a 1 h incubation with anti-goat/-rabbit HRP-conjugated secondary antibodies (1:5000, Agilent Dako) at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia and Astrocyte proliferation was visualized by staining the brain sections using anti Iba1 (1:2500; WAKO) and anti GFAP antibody (1:1000 Agilent technologies). When appropriate the brain sections were counterstained with haematoxylin and eosin ...
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bioRxiv - Physiology 2019Quote: Tendon fibroblasts were treated for 48 hours prior to being plated (8,000 cells) into collagen coated XFp cell culture miniplates (Seahorse Bioscience, Agilent, Santa Clara, CA). Cultured cells from each treatment condition were reseeded in duplicate ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded onto Mat-tek dishes at 200,000 cells/well in serum-free (low glucose) DMEM medium (Agilent Seahorse XF DMEM; 103575-100) supplemented with 4mM L-glutamine ...
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bioRxiv - Physiology 2020Quote: ... in HepG2 cell in response to 2MT was analyzed by the XF Cell Mito Stress TestTM using a Seahorse XFp analyzer (Seahorse Bioscience, North Billerica, MA) according to manufacturer’s instruction as described previously (Sumi et al. ...
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bioRxiv - Bioengineering 2024Quote: ... The diameter of cells adhered to TCP was measured by the Cytation 5 cell imaging multimode reader measurement tool (Agilent Technologies, Santa Clara, CA). In-suspension measurements were determined using the T4 Auto Cellometer Bright Field Cell Counter (Nexelom Bioscience ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were harvested from the T-75 flasks and seeded into a Seahorse 96-well XF Cell Culture microplate (Agilent Seahorse Bioscience, CA, USA) in 80 µL of the culture medium at a density of 20,000/well ...
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bioRxiv - Biophysics 2021Quote: ... coli XL10-Gold Ultracompetent Cells (Agilent Technologies, Santa Clara, CA) and miniprep plasmid DNAs were prepared from single transformants ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were maintained in a non-buffered assay medium (Agilent) in a non-CO2 incubator for 60 min before the assay ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were maintained in a non-buffered assay medium (Agilent) in a non-CO2 incubator for 60 min before the assay ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained with the anti-Ki-67 (M7240, Dako) and counterstained with DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... cells were equilibrated in unbuffered XFe assay medium (Seahorse Bioscience) supplemented with glucose (4.5 g/L) ...
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bioRxiv - Immunology 2020Quote: ... Absolute cell numbers were determined using CytoCount microspheres (Dako, Agilent). Total and surface expression analysis of MHC II ...
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bioRxiv - Immunology 2020Quote: ... Absolute cell numbers were determined using CytoCount microspheres (Dako, Agilent). Total and surface expression analysis of MHC II ...
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bioRxiv - Cell Biology 2022Quote: ... Using Seahorse XF Cell Mito Stress Test (Agilent, Santa Clara), fibroblasts were sequentially treated with 1,5 μM oligomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... Slides with cells were mounted with Fluorescence Mounting Medium (DAKO) and fluorescence signals were detected by microscopy (Zeiss Axio Scope.A1).
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bioRxiv - Neuroscience 2019Quote: ... were transfected into the AAV-293 cell line (Agilent, USA). The day before transfection ...
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bioRxiv - Plant Biology 2019Quote: ... Plasmids were transformed into BL21-DE3-RIPL competent cells (Agilent) and induced with 0.5 mM IPTG for 4 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Cells and tissue sections were mounted with mounting medium (Dako) and images acquired using a Leica TCS SP5 confocal microscope ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells were carried out in sealed serum vials (Agilent) maintained at a temperature of 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... AAV vectors were produced by transfection of AAV293 cells (Agilent) with three plasmids ...
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bioRxiv - Systems Biology 2021Quote: ... then inserted to electrocompetent E.coli cells (ElectroTen-Blue, Agilent Technologies) using electroporation ...
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bioRxiv - Microbiology 2021Quote: ... which was then transformed into XL10-Gold Ultracompetent Cells (Stratagene) for propagation.
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bioRxiv - Plant Biology 2021Quote: ... Expression plasmids were transformed into XL10-Gold Ultracompetent Cells (Agilent). DNA was extracted using ZR Plasmid Miniprep - Classic (Zymo Research ...
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bioRxiv - Cancer Biology 2022Quote: Agilent Seahorse XF Cell Mito Stress Test (Agilent Seahorse Bioscience) was applied to T98G cells according the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... coated Seahorse XF96 cell culture microplates (Agilent Technologies, 101085-004). Assay medium was prepared using Seahorse XF DMEM (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... T cells were plated in Seahorse XF DMEM Medium (Agilent) supplemented with 1% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: Agilent Seahorse XF Cell Mito Stress Test (Agilent Seahorse Bioscience) was applied to T98G cells according the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were acquired on a Novocyte flow cytometer (Agilent Technologies). For blood-derived monocytes phenotyping ...
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bioRxiv - Molecular Biology 2020Quote: ... prior to transformation with XL10-Gold competent cells (Agilent 200315):
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bioRxiv - Cell Biology 2021Quote: ... and cells were washed with phenol-free basal media (Agilent) and exposed to 150µL of minimal substrate assay media ...
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bioRxiv - Immunology 2020Quote: ... and Cell Mito Stress Test Kit (Agilent Technology, 103015-100) were used to measure the extracellular acidification rate (ECAR ...
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bioRxiv - Genetics 2022Quote: ... The cells were maintained in XF assay medium (Agilent, #102365100) and subjected to a mitochondrial stress test ...
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bioRxiv - Cancer Biology 2022Quote: ... Agilent Seahorse XF Cell Mito Stress Test (Agilent Seahorse Bioscience) was applied according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Agilent Seahorse XF Cell Mito Stress Test (Agilent Seahorse Bioscience) was applied according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were carefully washed with unbuffered DMEM (#103575-100, Agilent) supplemented with 10 mM glucose (#103577-100 ...
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bioRxiv - Biophysics 2022Quote: ... Construct was electroporated into E.coli (BL21-Gold Competend Cells) (Agilent Technologies ...