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Citations for Agilent :
201 - 250 of 7375 citations for Mouse Liver Expressed Antimicrobial Peptide 2 LEAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Peptide samples were desalted by OMIX C18 pipette tips (Agilent Technologies) and then analyzed by LC-MS/MS on an LTQ-Orbitrap velos instrument (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were then purified on a SampliQ SPE C18 cartridge (Agilent), vacuum dried and kept at −20 °C until further use.
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bioRxiv - Systems Biology 2022Quote: Peptides were prepared semi-automated on a Bravo pipetting robot (Agilent), similar as previously described 11 ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked peptides were enriched with Fe(III)-NTA cartridges (Agilent Technologies) using the Bravo AssayMAP robot (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide samples were desalted using OMIX C18 pipette tips (Agilent Technologies). The peptides mixtures were analyzed by nano-LC-MS/MS using an Ultimate 3000 system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The quality of the peptide was analyzed by HPLC (Agilent 1100) on a reverse-phase analytical C-18 column ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting peptides were trapped and desalted on C18 Zip-Tips (Agilent) and concentrated via speed-vaccum ...
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bioRxiv - Bioengineering 2023Quote: ... The peptide digests were extracted and desalted with C18 tip (Agilent) prior to liquid chromatography tandem mass spectrometry (LC MS/MS)54.
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bioRxiv - Immunology 2023Quote: ... Peptide clean-up was performed using AssayMAP C18 columns (Agilent Technologies) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... the peptides were purified using the Bond Elut OMIX tips (Agilent). The sample concentration was determined ...
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bioRxiv - Biochemistry 2024Quote: ... Tryptic peptides were desalted and concentrated with C18 microextraction tips (Agilent).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All liver RNA samples had integrity numbers (RIN) ≥ 7 as verified with the Agilent 2100 Bioanalyser (Agilent Technologies, Waldbronn, Germany). A260/A280 were ≥ 1.8 as verified with Thermo Scientific™ NanoDrop 2000 spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... VAT and liver samples were separated using a Zorbax Eclipse Plus C18 column (100mm x 2.1 mm x 1.8um, Agilent technologies Australia). Injection volume was kept at 1uL with a LC flowrate of 400 uL/min ...
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bioRxiv - Cancer Biology 2023Quote: Dual indexed whole exome capture libraries were prepared from mouse and naked mole-rat skin biopsies and matched livers using SureSelect XT HS and XT Low Input Enzymatic Fragmentation protocol (Agilent). For mouse whole exome sequencing (WES ...
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bioRxiv - Biochemistry 2020Quote: ... followed by horseradish peroxidase (HRP)-conjugated anti-mouse antibodies (Cat. N. P044701-2, Agilent Technologies LTD, Cheadle, UK) (2.4μg/mL ...
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bioRxiv - Immunology 2024Quote: ... sections were stained with monoclonal mouse anti-human CD68 clone PG-M1 - 1:100 (Dako Omnis: GA61361-2). Second antigen retrieval and blocking were performed as described above and subsequent staining was performed with either rabbit anti-Gas6 - 1:100 (PA5-79300 ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... while RNA quality expressed in RIN (RNA Integrity Number) was determined by electrophoresis using the 2200 TapeStation system (Agilent). Illumina mRNA sequencing (RNA-seq ...
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bioRxiv - Cell Biology 2019Quote: All constructs of FOR1 and PRF1 were expressed in BL21-Codon Plus (DE3)-RP (Agilent Technologies, Santa Clara, CA). Cdc12(FH1FH2 ...
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bioRxiv - Genetics 2022Quote: The wild-type and mutant BRD-1-BRC-1 chimeras were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). The cells were grown at 37°C until OD600 0.6 and were induced by 0.2mM isopropyl-β-D-thiogalactoside in the presence of 100μM ZnCl2 at 37°C for 6 hrs ...
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bioRxiv - Biochemistry 2023Quote: ... GST and GST-fused CBS-pair domain of murine CNNM2 were expressed in transformed BL21 (DE3) cells (Stratagene, CA). Bacterial cells were lysed by sonication in ice-cold PBS containing 1% Triton X-100 and protease inhibitor phenylmethylsulfonyl fluoride (Sigma–Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... Rat GluN2B-G689C-C1/2 and Rat GluN2B-G689S-C1/2 were generated using the QuikChange Site-Directed Mutagenesis Kit (Agilent,Cat. # 200518). Primers for GluN2B-G689C Mutagenesis ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit/mouse following manufacturer’s instructions (Detection Kit #K5005, Agilent Technologies, Santa Clara, CA, USA). Immunostained tissue sections were counterstained with hematoxylin solution according to Mayer (T865.1 ...
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bioRxiv - Immunology 2020Quote: Whole-exome sequencing libraries were constructed using the SureSelect Mouse all Exon Kit (Agilent). RNA sequencing libraries were constructed using the TrueSeq Stranded mRNA Sample Prep kit (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... the sections were incubated in HRP rabbit/mouse secondary antibody (DAKO Real EnVision kit) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from mouse lungs using the Absolutely Total RNA Purification Kit (Agilent). RNA extraction from cell culture experiments were performed using the Qiagen RNeasy kit (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... A series of alanine mutants were introduced into the SARS-CoV-2 spike protein using the QuickChange mutagenesis kit or the QuickChange multi-mutagenesis kit (Agilent). The primers for mutagenesis were designed on Agilent’s website (https://www.agilent.com/store/primerDesignProgram.jsp) ...
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bioRxiv - Cancer Biology 2021Quote: ... Mutations in genomic DNA from the spleen and liver were detected using the λ Select-cII Mutation Detection System for Big Blue Rodents (Stratagene) as we have done in the past (58).
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bioRxiv - Molecular Biology 2023Quote: ... The hybridization was performed according to the manufacturer instructions on 4×180K mouse microarrays (SurePrint G3 Mouse CGH Microarray Kit, 4x180K, AGILENT Technologies, reference genome: mm9). Microarrays were scanned with an Agilent High-Resolution C Scanner using a resolution of 3 µm and the autofocus option ...
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bioRxiv - Neuroscience 2021Quote: ... Mamu-DR (monoclonal mouse – anti-human HLA-DR/DQ-IgG1, clone CR3/43, cat. no. M077501-2, Agilent Technologies), 1:150 ...
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bioRxiv - Cell Biology 2020Quote: ... MBP-Tlg21-327 and the co-expressed Vps45–Tlg2 proteins were expressed and purified in a similar manner with the following modifications: protein was overexpressed in BL21-Codon Plus (Agilent); after IPTG addition the cells were grown at 16°C ...
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bioRxiv - Neuroscience 2021Quote: ... was fused with a six histidine tag (6xHis) and an AviTag (pET-28a-AviTag-GluN1C0) and expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). Expression of His-AviTag-GluN1(834-863 ...
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bioRxiv - Biophysics 2019Quote: Human CAMSAP1 residues 1474-1613 encompassing the CKK domain (HsCKK) were cloned into pET28a vector and expressed in BL21(DE3) cells (Stratagene). Following purification via immobilized metal-affinity chromatography (IMAC ...
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bioRxiv - Biochemistry 2021Quote: ... we generated MBP-PIPI (112.4 kDa) fusion gene on a pMAL-c5x vector and expressed it in Arctic Express DE3 (Agilent Technologies), a bacterial expression cell line that expresses psychrophilic chaperones which promote proper protein folding during low temperature growth ...
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bioRxiv - Biochemistry 2021Quote: Tpm isoforms Tpm1.6 and Tpm3.1 (with and without Met-Ala-Ser at the N-terminus) were expressed in ArcticExpress(DE3) RIL cells (Agilent Technologies), grown in Terrific Broth (TB ...
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bioRxiv - Biochemistry 2020Quote: ... Salmonella typhimurium MsbA (T561C in a C88A/C315A cysteine-less background) with an N-terminal poly-histidine-tag was expressed in BL21-CodonPlus (DE3)-RIPL (Agilent). Expression was induced with 1 mM IPTG for 4 hours at 30°C and membrane proteins were solubilized with 1% DDM and 0.04% sodium cholate in a buffer containing 100 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... CENP-A/H4 was expressed from a pST39 vector in BL21 (DE3) Codon Plus RIPL Escherichia coli (Agilent Technologies, 230280), grown in 2xYT medium (20 g/liter tryptone ...
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bioRxiv - Biophysics 2021Quote: hnRNPA1* (where * denotes that the hexa-peptide 259-264 is deleted) protein and deletion constructs were expressed as N-terminally tagged hSUMO fusion proteins in BL21 (DE3) RIPL cells (Agilent) in LB media ...
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bioRxiv - Plant Biology 2022Quote: His6-MBP-PFA-DSP protein fusions or free His8-MBP were expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells (Stratagene). Overnight bacterial cultures were inoculated 1:1000 into fresh 2YT medium (1.6 % tryptone ...
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Microbiology 2021Quote: The CAN97-83 HMPV P construct was cloned into the plasmid pET 302/NT-His between the cleavage sites EcoRI and XhoI and expressed in BL21(DE3) CodonPlus RIL cells (Agilent) overnight at 37 °C in terrific broth (TB ...
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bioRxiv - Genomics 2021Quote: The different Sen1 HD versions were fused to a C-terminal His8-tag coupled to Vibrio cholerae MARTX toxin cysteine protease domain (61)and expressed from the T7 promoter (see plasmids in table S6) in Escherichia coli strain BL21 (DE3) CodonPlus (Stratagene). Overexpression was induced by growth in auto-inducing medium (62 ...
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bioRxiv - Cell Biology 2020Quote: ... including glutathione S-transferase (GST)-NEPH1 cytoplasmic domain (CD) and GST-NEPHRIN CD were expressed and purified from Escherichia coli BL21 cells (Stratagene). Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene) ...
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bioRxiv - Biophysics 2020Quote: His-tagged CrSAS-6[NL] spanning amino acids 1-503 of the protein (see Supplementary Fig. 1a)1 was expressed in the Escherichia coli strain BL21(DE3) (Stratagene). Bacteria were grown at 37 °C in lysogeny broth (LB ...
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bioRxiv - Plant Biology 2022Quote: Plasmids were transformed and expressed in the Escherichia coli strain BL-21 (DE3) RIL cells (Agilent, Santa Clara, CA, USA). For protein expressions ...
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bioRxiv - Biochemistry 2023Quote: The gene for Pyrococcus horikoshii (Ph) LeuRS was synthesized by Gene Universal and expressed from pET-11a in BL21-CodonPlus (DE3)-RIPL (Agilent). Cells were grown at 37°C and induced with 300 µM IPTG for 4 hours then harvested and stored overnight at -20°C ...
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bioRxiv - Biophysics 2023Quote: GST-His9-SARS-CoV2 NTD-RBDL and NTDL-RBD Nucleocapsid constructs were expressed recombinantly in Gold BL21(DE3) cells (Agilent). 4 L cultures were grown in LB medium with carbenicillin (100 ug/mL ...
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Plant Biology 2023Quote: PHDsuper-FN3VRN5 from Zm for XL-MS analysis was inserted into pEC-LIC-His-3C containing a hexa-histidine tag and 3C protease cleavage site at the N terminus and was expressed in BL21 CodonPlus (DE3)-RIL cells (Agilent) in LB medium ...
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bioRxiv - Biophysics 2023Quote: Human MEK1 kinase domain (residues 1-393 with a TEV protease cleavable N-terminal 6x-His tag in pET-Duet) containing an inactivating K97R mutation was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...