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Citations for Agilent :
201 - 250 of 4446 citations for Dendritic Cell Specific Transmembrane Protein DCSTAMP Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... sections were incubated overnight at 4 ⁰C with a cocktail of the primary antibodies αSyn (clone KM51 1:500, Monosan Xtra, The Netherlands) and glial fibrillary acidic protein (GFAP, Z0334, 1:4000, DAKO, Denmark). After rinsing in PBS ...
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bioRxiv - Neuroscience 2024Quote: Mouse hind paws were fixed in Zamboni’s fixative for 24-48 hours at 4°C and processed as previously described.44 50 μm floating tissue sections were stained with the primary antibody rabbit anti-protein-gene-product 9.5 (PGP 9.5) (Dako, Z5116, 1:500) overnight and the secondary biotinylated goat anti-rabbit IgG antibody (Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies). Lager scale transient transfections were purified on the Akta FPLC system using 1mL HiTrap MabSelect PrismA™ affinity chromatography resin (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated with anti-VP1 antibodies (Dako, anti-enterovirus clone5-D8/1) diluted 1:500 in blocking solution for detection of viral protein VP1 or anti dsRNA antibody (SCICONS ...
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bioRxiv - Neuroscience 2024Quote: ... Proliferating cells were nuclear labeled with the Ki67 antibody (1:200, Dako GA626). The primary antibody binding was visualized using a biotinylated secondary antibody followed by developing with avidin-peroxidase and diaminobenzidine or 3-amino-9-ethylcarbazole.
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... cDNA sequencing libraries were then prepared using the SureSelect Strand-Specific RNA library preparation kit (Agilent Technologies) with a modified protocol omitting the polyA selection step ...
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bioRxiv - Plant Biology 2022Quote: ... The mRNA-seq libraries were constructed by using SureSelect Strand Specific RNA Library Prep kit (Agilent Tech.) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... Promoter-specific Capture Hi-C was performed using the SureSelect XT Library Prep Kit ILM (Agilent Technologies), which included a custom-designed biotinylated RNA bait library and paired-end blockers ...
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bioRxiv - Biochemistry 2024Quote: The specific activity of SfC12DO was determined spectrophotometrically using a Cary 60 UV-vis spectrophotometer (Agilent Technologies). The enzymatic reaction was monitored by measuring the formation of product (ccMA ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was performed using the streptavidin-biotin-peroxydase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Slides were finally counterstained with haematoxylin ...
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bioRxiv - Biochemistry 2020Quote: Skd3 variants were expressed as an N-terminally MBP-tagged protein in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in 40mM HEPES-KOH pH=7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... cells or tissue sections were incubated with Protein Block Serum-Free Ready-To-Use (Dako, #X 0909) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... A53T and E46K α-synuclein proteins were expressed in BL2-Gold(DE3) competent cells (Agilent, Cat #230132). Protein expression was induced at log phase with 200 μM IPTG for 16 hours at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Protein expression was conducted in E.coli BL21 (DE3) Codon Plus cells (Agilent Technologies, San Diego, CA, USA). Induced cultures were grown at 18° C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... the co-cultures were incubated first with primary antibody for glial fibrillary acidic protein (GFAP, 1:400, Z033429-2, Dako, Glostrup, Denmark) prepared in 5% NGS in DPBS overnight at +4 following an incubation with Alexa Fluor405 (A31556 ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Immunology 2022Quote: ... CD45RA+ naive cells were separated from the CD45RO+ memory T cells by positive selection of memory T cells using CD45RO-Phycoerythrin antibody (DAKO) and magnetically-labeled anti-PE beads (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2021Quote: ... The mutants were created by the same method for site-directed mutagenesis using specific primers designed from Agilent QuickChange Primer design web tool ...
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bioRxiv - Cancer Biology 2020Quote: ... Strand specific RNA sequencing was performed on quality controlled high RIN value (>7) RNA samples (Bioanalyzer Agilent Technologies). In brief ...
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bioRxiv - Synthetic Biology 2022Quote: Specific biosensors identified during the selection and subsequent CleaveSeq analysis were resynthesized on an oligonucleotide array (Agilent, G7220A). The array contained 1,730 sequences each padded to a length of 158 nt ...
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bioRxiv - Immunology 2023Quote: ... The LCMV-specific antigen tetramer was prepared by conjugating the biotinylated LCMV-NP to SA-PE (Prozyme PJRS25) at a concentration ratio of 4:1 respectively ...
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bioRxiv - Microbiology 2024Quote: ... Env mutants with specific changes were generated by using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies). All the Envs contain a His6 tag at the carboxyl terminus ...
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bioRxiv - Cell Biology 2021Quote: ... melanin-fed cells were stained with anti-PMEL antibody (HMB45) (Agilent DAKO, CA, USA), to allow the detection of internalized melanin by confocal microscopy and quantification using the Spot Detector plugin from Icy Software (https://icy.bioimageanalysis.org).
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bioRxiv - Cell Biology 2021Quote: ... melanin-fed cells were stained with anti-PMEL antibody (HMB45) (Agilent DAKO, CA, USA), to allow the detection of internalized melanin by confocal microscopy and quantification using the Spot Detector plugin from Icy Software (https://icy.bioimageanalysis.org).
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bioRxiv - Neuroscience 2023Quote: ... The cells were then incubated with primary antibody to GFAP (1:1000, #Z0334, DAKO), S100b (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... immunohistochemistry was performed by using the streptavidin-biotin-peroxidase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Negative controls were obtained after omission of the primary antibody or incubation with an irrelevant antibody.
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bioRxiv - Cancer Biology 2021Quote: Biopsy sections were deparaffinized and antigens were retrieved by boiling in 10mM sodium citrate buffer pH6 for 40 minutes followed by endogenous biotin blocking (Agilent) and normal goat serum blocking (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... incubated with Dako biotin (cat. no. X0590) and peroxidase (cat. no. S200389) blocking reagents (both Agilent, Santa Clara, CA, USA), and incubated with primary antibodies (diluted in TBS-T-BSA ...
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bioRxiv - Cancer Biology 2020Quote: EGFR-KD from Expi293 cell lysates was bound to streptavidin resin and eluted in biotin buffer according to manufacturer’s recommendations (InterPlay Mammalian TAP System; Agilent Technologies). Typical protein yield was between 100-400 ng/µl ...
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bioRxiv - Immunology 2020Quote: ... protein block (Dako X0909) for 20 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... and Protein block (Dako) before staining with anti-pS6 (detected with fluorochrome conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein block (DAKO) were conducted before a 1-hour primary antibody incubation at room temperature ...
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bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2024Quote: GST-fusion proteins were expressed in BL21-Codon Plus strain of Escherichia coli cells (Agilent, Santa Clara, CA) and purified with glutathione-Sepharose beads (GE Healthcare Life Sciences ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then incubated for 1 hour at room temperature with rabbit anti-glial fibrillary acidic protein (GFAP; rabbit anti-GFAP antibody, 1:300, Dako, Z0334, Carpinteria, CA), rabbit anti-oligodendrocyte specific protein (OSP ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were processed as described and probed with a set of 264 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA). Each slide was incubated with one specific primary antibody and a negative control slide was incubated with antibody diluent without any primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 1h at RT before overnight incubation at 4°C with primary antibodies: Glial Fibrillary Acidic Protein (GFAP) (1/500 Dako Cat. Number Z0334); AQP4 (1/500 ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were processed as described and probed with a set of 258 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA). Each slide was incubated with one specific primary antibody and a negative control slide was incubated with antibody diluent without any primary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... was performed by LC/MS/MS by an MRM approach using the specific transition of cGMP (m/z 346.0 → 152.0) (1200 HPLC system (Agilent Technologies) coupled to an LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Specific point mutations of RanGAP1 were introduced by site-directed mutagenesis with a QuikChange Site-Directed Mutagenesis Kit (Stratagene).
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bioRxiv - Genomics 2022Quote: ... RT was performed with a specific primer (5′-CCTACACGACGCTCTTCC-3′) using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). RNA degradation was performed by incubating the RT mixture with 10% 1 M NaOH (2μl of RT mixture ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PKR site-specific mutants and epteK3Δ227-508 were generated by PCR mutagenesis using the QuickChange Lightning mutagenesis kit (Agilent) and primers holding the desired mutations/deletions ...
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bioRxiv - Cancer Biology 2022Quote: ... PLK1 mutant plasmids were generated using specific primers (sequences available upon request) following the manufacturer’s instructions (QuickChange II, Stratagene). shPLK1 UTR ...
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bioRxiv - Molecular Biology 2022Quote: ... Extracted ion chromatograms of the compound-specific mass transitions were integrated using MassHunter software (Agilent, Santa Clara, CA, USA). Absolute concentrations were calculated based on an external calibration curve.
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bioRxiv - Evolutionary Biology 2024Quote: ... The specific process was as follows: Chromatographic elution was performed on an Agilent 1290 infinity system (Agilent technologies, USA) equipped with a sample manager coupled to a mass spectrometry with an electrospray ion source in both positive and negative ion modes ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... was used for the RNA quality control with a microfluidic chip specific for RNA (Agilent RNA 6000 Nano Kit). Then 1 μg of qualified RNA samples in RNase-free ddH2O was used for library preparation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the assay medium was prepared by supplementing Seahorse XF Base medium (pH 7.4) with a specific combination of 10 mM glucose (100X stock, Agilent), 1 mM pyruvate (100X stock ...
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bioRxiv - Immunology 2023Quote: ... site-directed mutagenesis using specific primers was performed based on the QuikChange site-directed mutagenesis Kit II (Agilent Technologies) according to the manufacturer’s instructions ...