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Citations for Agilent :
2051 - 2100 of 2391 citations for ERK1 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: All experiments used 1.5 μL packed bed volume Exp 2 bolt cartridge trapping columns (Optimize Technologies) packed with PLRP (Agilent) solid phase and 150 x 0.3 mm analytical columns packed with 2.6 μm Kinetex core shell particles coated with polar capped C18 (Phenomenex) ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Oxygen consumption rates (OCR ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with this media for 4 hours before plating with the assay media (composed of XF Base Medium Minimal DMEM supplemented with 2 mM glucose and 0.5 mM L-carnitine; Agilent) in the XF96 well plate (Agilent ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4 °C followed by a 1 h incubation with either an HRP-conjugated goat anti-mouse IgG or goat anti-rabbit IgG (Dako, Cambridge, UK). Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Subsequent antibody incubation was performed at 4°C overnight or for one hour at room temperature using Dako polyclonal goat anti-rabbit or anti-mouse immunoglobulins/HRP (Agilent Technologies, USA). Membranes were washed using 0.1% TBS-T and proteins were detected using ECL or ECL Select (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight at 4° C with primary antibodies (mouse-anti-Ataxin-3, 1:2500, clone 1H9, MAB5360, MerckMillipore, Darmstadt, Germany; rabbit-anti-Ubiquitin, 1:500, Z0458, Dako, Jena, Germany). After washing the membranes were incubated with secondary antibodies (peroxidase affinePure donkey anti-mouse IgG H+L ...
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bioRxiv - Neuroscience 2020Quote: ... blocked using 10% Normal Goat Serum (NGS) and incubated overnight with the following primary antibodies in 5% NGS overnight at 4°C: rabbit anti-GFAP (DAKO; #Z0334;1:1000), rat anti-L1-CAM (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Sections labelled for F4 were incubated with secondary antibody from kit polymer-HRP anti-rabbit (Dako En Vision+ System-HRP, Dako, Glostrup, Denmark) while sections labelled for CD3 were incubated with anti-mouse biotinylated secondary antibody (catalogue number BA-2000-1.5 Vector Laboratories ...
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bioRxiv - Pathology 2021Quote: ... the sections were incubated with biotinylated secondary anti-rabbit IgG antibody and stained using Dako Liquid DAB+ Substrate Chromogen System (Dako, Glostrup, Denmark).
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bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... IgG1a monoclonal antibody in PBS with 1% BSA followed by a bridge step labelling with a polyclonal Rabbit Anti-Mouse Immunoglobulins (Dako, 1:50) and protein A-gold 10 nm (UMC Utrecht ...
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bioRxiv - Neuroscience 2021Quote: ... EnzoLife Science, 1:1000), anti-tubulin (mouse, available inhouse, 1:10000) and HRP-conjugated secondary antibodies anti-rabbit (goat, Dako, 1:2000), anti-mouse (goat ...
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bioRxiv - Pathology 2021Quote: ... were blocked in antibody selection buffer and used to immobilize 10-15μg polyclonal rabbit anti-VWF (Dako A0082, lots 00045319 and 00051141) or immunoglobulin G (IgG ...
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bioRxiv - Pathology 2021Quote: ... the sections were incubated with biotinylated secondary anti-rabbit IgG antibody and stained using Dako Liquid DAB+ Substrate Chromogen System (Dako, Glostrup, Denmark). Cell nuclei were stained with hematoxylin ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated for 1 h at room temperature with rabbit anti-glial fibrillary acidic protein (GFAP, 1:300; Dako, Carpinteria, CA), rabbit anti-oligodendrocyte specific protein (OSP ...
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bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... primary antibody was produced in-house from HB 65 mouse anti-nucleoprotein and diluted 1:2500 followed by HRP-conjugated rabbit anti-mouse diluted 1:500 (Dako, Glostrup, Denmark). The titration was repeated on a different day and TCID50 titres were calculated using Reed and Muench (29).
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP, Dako, 1:2500), and washed 3 times 15 minutes in TBST again before chemiluminescence detection ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were then treated with biotinylated secondary antibodies and target staining was performed with peroxidase-conjugated streptavidin and DAB chromogen (Dako REAL Detection System Peroxidase/DAB+, Rabbit/Mouse Kit; Dako, Glostrup, Denmark). Mayer’s hematoxylin (Carl Roth ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour at room temperature in the HRP-labelled polyclonal goat anti-rabbit IgG (DAKO, cat. P0448) or HRP-labelled polyclonal goat anti-mouse IgG (DAKO ...
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bioRxiv - Cancer Biology 2024Quote: ... at 1:400 for two hours and then incubated for 30 minutes with Rabbit Envision HRP System reagent (Agilent, Santa Clara, CA). For vimentin staining ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with secondary antibodies diluted in 0.5% WBR in TBST for 1 hour at room temperature (Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP, Agilent Dako #P0448, 1/2000), washed three times with TBST ...
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Treatment with furosemide indirectly increases inhibitory transmission in the developing hippocampusbioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP, Dako, 1:2500), and washed 3 times 15 minutes in TBST again before chemiluminescence detection ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Immunology 2023Quote: ... An anti-rabbit IgG horseradish-peroxidase conjugated (1/2000 dilution) was used as a secondary antibody (Agilent DAKO, Santa Clara, CA, USA) and 4-Chloro-1-Naphthol as substrate for colorimetric detection.
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal rabbit anti-mouse secondary antibody IgG/HRP (P0260; Agilent Technologies/Dako) diluted 1:2000 in 3% BSA.
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bioRxiv - Immunology 2023Quote: ... After incubation with the rIgE’s the wells were washed four times with ELISA wash buffer and incubated with 100 μL of 1,3 μg/mL rabbit anti-human IgE-conjugated HRP (DAKO, cat no: P0295) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... An anti-rabbit IgG horseradish-peroxidase conjugated (1/2000 dilution) was used as a secondary antibody (Agilent DAKO, Santa Clara, CA, USA) and 4-Chloro-1-Naphthol as substrate for colorimetric detection.
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bioRxiv - Neuroscience 2023Quote: ... sympathetic ganglia and spinal cords was conducted directly on the slides using primary rabbit anti-HSV-1 (1:100; B0114, DakoCytomation, Carpinteria, CA) and chicken anti-GFP (1:400 ...
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bioRxiv - Plant Biology 2020Quote: ... The digest was filtered (0.22 μm) and filtered samples (1-2 μg) were loaded onto a C18 high capacity nano LC chip (Agilent Technologies) using a 1200 series capillary and eluted directly into a 6550 series quadrupole time-of-flight mass spectrometer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... 2×105 cells/well were plated in five technical replicates on collagen-coated XF96 polystyrene cell culture microplates (Seahorse, Agilent) in GM ...
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of each purified final library was run on an Agilent TapeStation HS D1000 ScreenTape (Agilent Technologies, 5067-5584). The libraries were quantified using the Quant-iT 1X dsDNA HS kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the cells were resuspended in 500 µl PBSF buffer (PBS+2% FBS) and analyzed using ACEA NovoCyte TM (Agilent Biosciences); non-transfected 293T cells served as a negative control.
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bioRxiv - Neuroscience 2021Quote: ... We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250, M077501-2, Agilent, UK), rabbit anti-PU.1 (1:200 ...
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bioRxiv - Molecular Biology 2020Quote: Per2AS variant 2 overexpression plasmid was generated from 5’RACE and 3’RACE products that were cloned in to pBluescript (Agilent), as well as Per2AS qPCR product cloned into pGEM-T (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... Before separation peptides were first trapped (Dr Maisch Reprosil C18, 3 μm, 2 cm x 100 μm) and then separated on an analytical column (Agilent Poroshell EC-C18 ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of labeled cRNA was hybridized with a human gene expression microarray 60K (Agilent Technologies, Santa Clara CA, USA). The microarray workflow quality control was implemented using the Agilent Spike-In Kit which consisted of a set of 10 positive control transcripts optimized to anneal to complementary probes on the microarray with minimal self-hybridization or cross-hybridization ...
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bioRxiv - Microbiology 2021Quote: ... D614G and other SARS-CoV-2 Spike single mutations were generated using the QuickChange II XL site-directed mutagenesis protocol (Stratagene) and the pCG1-SARS-CoV-2-S plasmid kindly provided by Stefan Pöhlmann ...
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bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
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bioRxiv - Biophysics 2020Quote: ... They were then stained for 5 min with 4,6-diamidino-2-phenylindole (DAPI) and mounted using fluorescence mounting medium (DAKO, Agilent). Pictures were taken with an UltraVIEW ERS spinning disk confocal microscope (Zeiss 63X/1.4 ...
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bioRxiv - Cell Biology 2021Quote: RNAi-resistant EGFP-ORP5A and EGFP-ORP5B were generated by introducing 4 silent point mutations in the region targeted by the 2 siRNA oligos (#10 and #11) by site-directed mutagenesis (Quickchange II-XL, Stratagene) and the following primers:
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bioRxiv - Biochemistry 2020Quote: ... Radioactivity was detected using an in-line Lablogic Radiodetector and the analysis was calibrated using a co-injected 2-aminobenzamide-labeled dextran ladder (ProZyme/Agilent) detected on a Dionex fluorescence detector.
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bioRxiv - Plant Biology 2021Quote: ... The SEGS-2 ATG mutant (pNSB2110) was created in S2-1.5b (pNSB1869) using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and the primers ...
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bioRxiv - Plant Biology 2021Quote: ... pGEX6P1:SYT1C2B (residues G374-end) and pGEX6P1:SYT1-SMPC2A (residues N34-E397) were transformed into Rosetta 2 (DE3) pLysS cells (Stratagene) for protein expression using standard protocols ...
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bioRxiv - Microbiology 2021Quote: ... 2□μl of each sample was used to assess RNA quality using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA), and the RNA integration numbers (RIN ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation and the combination of mutations found in SARS-CoV-2 variants were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana vesicular stomatitis virus (VSV ...
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bioRxiv - Physiology 2021Quote: ... Peptides were first trapped (Dr. Maisch Reprosil C18, 3 μm, 2 cm × 100 μm) prior to separation on an analytical column (Agilent Poroshell EC-C18 ...
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bioRxiv - Microbiology 2020Quote: ... Equal number of live cells was seeded at 2*105 cells per well in XF96 V3 PS plates (Seahorse Bioscience) precoated with 0.5 mg/ml Corning® Cell-Tack™ Cell and Tissue Adhesive (Corning ...