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Citations for Agilent :
1951 - 2000 of 2297 citations for ERK1 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... following antigen retrieval by boiling for 20 min in target retrieval solution (pH 6.0, Agilent Dako, Cat # 169984-2) and blocking steps ...
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bioRxiv - Immunology 2020Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Plant Biology 2021Quote: ... and 2% Buffer B (0.1% formic acid in Optima grade acetonitrile (Fisher)) using a 1200 series capillary pump (Agilent). Following loading ...
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bioRxiv - Neuroscience 2021Quote: ... Mamu-DR (monoclonal mouse – anti-human HLA-DR/DQ-IgG1, clone CR3/43, cat. no. M077501-2, Agilent Technologies), 1:150 ...
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bioRxiv - Immunology 2021Quote: ... total RNA from PBMC’s from 2 ml of blood was extracted using AllPrep DNA/RNA Micro Kit from Agilent Technologies (Cat ...
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bioRxiv - Genetics 2020Quote: ... Baseline respiration and maximal respiratory capacity were measured using the Seahorse instrument (XF24 data for Figure 2 and Xe96 data for figure 3) according to manufacturer’s instructions (Agilent). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... Detection of the primary antibodies was carried out using Envision FLEX/HRP secondary antibody (GV80011-2; DAKO, Hamburg, Germany). 3,3′-diaminobenzidine (DAB ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 20 embryos were placed into a clean 2 mL amber vial (Cat. No. 5182-0558, Agilent, Santa Clara, CA) and residual egg water was removed ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Mpro mutants were generated with QuikChange® II Site-Directed Mutagenesis Kit from Agilent (Catalog #200524), using plasmid pE-SUMO-Mpro as the template ...
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bioRxiv - Evolutionary Biology 2023Quote: We sheared DNA to a mean size distribution of ∼20 kb using a Diagenode Megaruptor 2 according to the manufacturer’s protocol, (Denville, New Jersey, USA) and sized sheared DNA on a Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Immunology 2023Quote: ... at a density of 2×105 cells/well on a Seahorse XFe96 cell culture plate (Agilent Technologies; 101085-004). Plates were pre-coated with Corning™ Cell-Tak Cell and Tissue Adhesive (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was determined during basal and upon addition of oligomycin (1 µM), carbonylcyanide p-(trifluoromethoxy) phenylhydrazone (FCCP, 2 µM) and rotenone/antimycin (0.5 µM) using the Seahorse XFe24 bioanalyzer (Agilent). Data from each well was corrected to cell number using the Bio Tek Cytation 1 Cell Imaging Multimode Reader and presented as mean +/-SEM.
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bioRxiv - Neuroscience 2023Quote: ... These pUAST-(G4C2)n vectors were amplified with a recombinase-mutated SURE®2 Escherichia coli strain (Agilent Technologies) at 28 °C for 72 hours to prevent repeat length contraction ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions contained 10 µL of 2× Brilliant III Ultra-Fast SYBR green QPCR master mix (catalog no. 600882; Agilent), 2 µL each of 2 µM PCR primers (see Table S1 for used primers) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were acidified with TFA to a pH < 2 and desalted by C18 using the Bravo AssayMAP platform (Agilent) before enrichment of crosslinked peptides with Fe(III)-NTA cartridges in the Bravo AssayMAP platform as previously described[23].
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bioRxiv - Genetics 2023Quote: ... resulting from Cas12a based trans-cleavage were measured every 2.5 min using a BioTek Synergy 2 microplate reader (Agilent). In a diagnostic specificity assay using field samples ...
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bioRxiv - Physiology 2023Quote: ... followed by Vectastain Elite ABC HRP detection kit (Vector PK-6100) and detection with DAB (Agilent Technologies K346811-2). Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were washed with 0.1% Triton X-100 in PBS and the slides were mounted in fluorescence mounting medium (S302380-2, Agilent). Imaging was performed with Cell Observer Spinning Disk Confocal Microscope using the ZEN software ...
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bioRxiv - Cancer Biology 2023Quote: ... All libraries were sequenced (2×100 bp) using Agilent SureSelect RNA XTHS2 All Exon V8 (Agilent, cat. no. G9991A) according to the standard Agilent protocol.
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bioRxiv - Molecular Biology 2023Quote: ... Following permeabilization the cells were washed three times with PBS then blocked with Protein Block (Aligent/Dako, X090930-2). Primary antibodies (ADAR1 (Santa Cruz ...
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bioRxiv - Bioengineering 2023Quote: ... on a Shimadzu LC2030C Plus 2 machine equipped with a Poroshell 120EC-C18 column (250 x 4.6 mm, Agilent) and a UV/vis detector ...
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bioRxiv - Developmental Biology 2024Quote: ... the slides were treated with the Dako Real Envision Detection System kit (K500711-2, Agilent, Santa Clara, CA, USA) and counterstained with hematoxylin.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... the buffer was exchanged into basic PBS (pH 8.0) with 0.1 % Tween-20 for a 2 h conjugation with 10 µM streptavidin (Prozyme, SA10) and 10 µM fluorescent dye (Lissamine Rhodamine B ethylenediamine ...
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bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4 °C followed by a 1 h incubation with either an HRP-conjugated goat anti-mouse IgG or goat anti-rabbit IgG (Dako, Cambridge, UK). Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Subsequent antibody incubation was performed at 4°C overnight or for one hour at room temperature using Dako polyclonal goat anti-rabbit or anti-mouse immunoglobulins/HRP (Agilent Technologies, USA). Membranes were washed using 0.1% TBS-T and proteins were detected using ECL or ECL Select (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight at 4° C with primary antibodies (mouse-anti-Ataxin-3, 1:2500, clone 1H9, MAB5360, MerckMillipore, Darmstadt, Germany; rabbit-anti-Ubiquitin, 1:500, Z0458, Dako, Jena, Germany). After washing the membranes were incubated with secondary antibodies (peroxidase affinePure donkey anti-mouse IgG H+L ...
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bioRxiv - Neuroscience 2020Quote: ... blocked using 10% Normal Goat Serum (NGS) and incubated overnight with the following primary antibodies in 5% NGS overnight at 4°C: rabbit anti-GFAP (DAKO; #Z0334;1:1000), rat anti-L1-CAM (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Sections labelled for F4 were incubated with secondary antibody from kit polymer-HRP anti-rabbit (Dako En Vision+ System-HRP, Dako, Glostrup, Denmark) while sections labelled for CD3 were incubated with anti-mouse biotinylated secondary antibody (catalogue number BA-2000-1.5 Vector Laboratories ...
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bioRxiv - Pathology 2021Quote: ... the sections were incubated with biotinylated secondary anti-rabbit IgG antibody and stained using Dako Liquid DAB+ Substrate Chromogen System (Dako, Glostrup, Denmark).
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bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... IgG1a monoclonal antibody in PBS with 1% BSA followed by a bridge step labelling with a polyclonal Rabbit Anti-Mouse Immunoglobulins (Dako, 1:50) and protein A-gold 10 nm (UMC Utrecht ...
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bioRxiv - Neuroscience 2021Quote: ... EnzoLife Science, 1:1000), anti-tubulin (mouse, available inhouse, 1:10000) and HRP-conjugated secondary antibodies anti-rabbit (goat, Dako, 1:2000), anti-mouse (goat ...
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bioRxiv - Pathology 2021Quote: ... were blocked in antibody selection buffer and used to immobilize 10-15μg polyclonal rabbit anti-VWF (Dako A0082, lots 00045319 and 00051141) or immunoglobulin G (IgG ...
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bioRxiv - Pathology 2021Quote: ... the sections were incubated with biotinylated secondary anti-rabbit IgG antibody and stained using Dako Liquid DAB+ Substrate Chromogen System (Dako, Glostrup, Denmark). Cell nuclei were stained with hematoxylin ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated for 1 h at room temperature with rabbit anti-glial fibrillary acidic protein (GFAP, 1:300; Dako, Carpinteria, CA), rabbit anti-oligodendrocyte specific protein (OSP ...
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bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... primary antibody was produced in-house from HB 65 mouse anti-nucleoprotein and diluted 1:2500 followed by HRP-conjugated rabbit anti-mouse diluted 1:500 (Dako, Glostrup, Denmark). The titration was repeated on a different day and TCID50 titres were calculated using Reed and Muench (29).
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP, Dako, 1:2500), and washed 3 times 15 minutes in TBST again before chemiluminescence detection ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were then treated with biotinylated secondary antibodies and target staining was performed with peroxidase-conjugated streptavidin and DAB chromogen (Dako REAL Detection System Peroxidase/DAB+, Rabbit/Mouse Kit; Dako, Glostrup, Denmark). Mayer’s hematoxylin (Carl Roth ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour at room temperature in the HRP-labelled polyclonal goat anti-rabbit IgG (DAKO, cat. P0448) or HRP-labelled polyclonal goat anti-mouse IgG (DAKO ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Immunology 2023Quote: ... An anti-rabbit IgG horseradish-peroxidase conjugated (1/2000 dilution) was used as a secondary antibody (Agilent DAKO, Santa Clara, CA, USA) and 4-Chloro-1-Naphthol as substrate for colorimetric detection.
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal rabbit anti-mouse secondary antibody IgG/HRP (P0260; Agilent Technologies/Dako) diluted 1:2000 in 3% BSA.
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bioRxiv - Immunology 2023Quote: ... After incubation with the rIgE’s the wells were washed four times with ELISA wash buffer and incubated with 100 μL of 1,3 μg/mL rabbit anti-human IgE-conjugated HRP (DAKO, cat no: P0295) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... An anti-rabbit IgG horseradish-peroxidase conjugated (1/2000 dilution) was used as a secondary antibody (Agilent DAKO, Santa Clara, CA, USA) and 4-Chloro-1-Naphthol as substrate for colorimetric detection.