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Citations for Agilent :
1901 - 1950 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and the quality as well as yield of fully-barcoded DNA primers was evaluated with total RNA Pico Assay (Agilent Technologies, 5067-1513) and by fluorescent in situ hybridization (FISH ...
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bioRxiv - Molecular Biology 2024Quote: ... library yields were determined using the Qubit 2.0 DNA HS Assay and the quality of the libraries generated was assessed using the TapeStation HSD1000 ScreenTape (Agilent Technologies Inc., California, USA). The final libraries quantity was assessed by APA SYBR® FAST qPCR with QuantStudio® 5 System (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... or flurometric (citrate, acetyl-CoA) assays (cat. no. KA1674, ab102516, KA3791 and MAK039, respectively) and a microplate reader (BioTek Synergy Neo2, Agilent, Santa Clara, CA) according to manufacturer’s instructions (Abnova ...
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bioRxiv - Physiology 2023Quote: ... and quality was assessed for a subset of RNA isolates using the Bioanalyzer RNA 6000 Pico Chip assay (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Microbiology 2023Quote: ... Each sample was sized by the Gel fragment analyzer instrument using the DNF-474 High Sensitivity NGS assay (Agilent Technologies #DNF-474-0500) to calculate the average fragment size (expected fragment size of ∼600bp ...
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bioRxiv - Biochemistry 2023Quote: ... Assays were performed on an Agilent 1100 series HPLC with a reverse phase column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 × 150 mm). Solvent A was water containing 0.1% TFA and solvent B was acetonitrile containing 0.1% TFA ...
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bioRxiv - Genomics 2023Quote: ... using a Covaris ME220 ultrasonic shearing system to a median size of ∼300 bp as determined by the Agilent 2100 High Sensitivity Assay (Agilent, Santa Clara, CA). Libraries were generated using the DuplexSeq Rat-50 Mutagenesis Assay library preparation kit and protocol using version 1.0 chemistry as described above ...
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bioRxiv - Biochemistry 2024Quote: ... Assay plates were generated by plating 2 µL of the deep well stock plates into 384-well assay plates (Thermo Scientific #142761 using the Agilent V11 Bravo). 88 µL of SrEpac cultured in high-nutrient media (5% Sea Water Complete ...
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bioRxiv - Immunology 2024Quote: ... Amplified products were SPRI purified with a 0.6X bead ratio and analyzed on a Tapestation using the High Sensitivity D5000 Screentape assay (Agilent, Cat No. 5067-5592) to assess quality.
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bioRxiv - Immunology 2024Quote: ... HER2 CAR-T cells or mock CAR-T cells from the same donor were added at an effector:target cell ratio of 10:1 and measured in a real-time cell analysis assay using the xCELLigence SP system (Agilent, Santa Clara, CA). An electrical current was applied to each co-culture ...
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bioRxiv - Evolutionary Biology 2024Quote: The enzymatic activity of both IPT MAOs was measured by a spectrophotometric assay in the presence of varying concentrations of substrates using a Cary 100 UV/Vis spectrophotometer (Agilent Technologies, CA, USA) (Reis and Binda ...
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bioRxiv - Bioengineering 2024Quote: ... centrifuged at 1000 x g for 5 minutes and resuspended in 2 mL of assay media (Seahorse XF base medium without phenol red (Agilent, Cat. # 103334-100), 1 mM pyruvate (Acros Organics ...
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bioRxiv - Cancer Biology 2024Quote: ... 2×105 neutrophils were resuspended in 50 µL neutrophil mitochondria assay media containing Seahorse Agilent RPMI media (Agilent Technologies, Cat. #103576-100, US), 1 mM pyruvate ...
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bioRxiv - Genomics 2020Quote: ... RNA was isolated using the RNeasy MinElute kit as per manufacturer’s instructions and was quantified using the Agilent Tapestation High Sensitivity RNA kit (Agilent 067-5576). RNA sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and 75 base-pair single-end reads were sequenced using the Illumina NEXTseq 500 ...
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bioRxiv - Immunology 2022Quote: ... Human B Cell Kit (10X Genomics) and the quality and quantitation of the products were monitored using the High Sensitivity DNA Kit (Agilent Technologies). Libraries were sequenced on an Illumina sequencer with Illumina adaptor primers found in the Chromium i7 Multiplex Kit.
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bioRxiv - Systems Biology 2020Quote: ... Total RNA was isolated from snap-frozen cells by using Quiagen AllPrep DNA/RNA Mini Kit (see simultaneous purification of DNA and RNA) and quantified using Agilent RNA 6000 Nano Kit (Agilent Technologies) on the Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2021Quote: ... IL17A and IL23 using Affinity QPCR cDNA Synthesis kit and Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix as per kit instructions (Agilent Technologies Inc. ...
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bioRxiv - Cell Biology 2021Quote: The bioenergetic profile of BMDMs were assessed by measuring the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using a XF cell Mito Stress Test kit and XF glycolysis stress kit (Agilent Technologies) as per manufacturer’s instructions using a Seahorse XF extracellular flux analyzer (Agilent technologies ...
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bioRxiv - Microbiology 2020Quote: ... The library concentrations were measured using the Qubit™ dsDNA HS Assay Kit and the library insert size determined by the Agilent DNA 1000 Kit in a 2100 Electrophoresis Bioanalyser Instrument (Agilent, USA).
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bioRxiv - Immunology 2020Quote: ... RNA and cDNA quantity and quality were assessed using the Agilent RNA 6000 Nano Kit and Agilent RNA 6000 Pico Kit according to the manufacture’s protocol (Agilent Technologies, USA). Labelled cDNA were hybridised on a MouseWG-6 v2.0 Expression BeadChip (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was stored at −80°C and RNA quality was assessed by Bioanalyzer (Agilent 2100 Bioanalyzer, RNA 6000 Nano Kit for cells, RNA 6000 Pico Kit for EVs, Agilent Technologies).
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bioRxiv - Microbiology 2023Quote: ... Specific mutant constructs were subcloned from the mutants using appropriate restriction digestion sites or using the site-directed mutagenesis kit (Quick Change II Site directed mutagenesis kit, Agilent Technologies) following manufacturers protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... as described previously (67) by using the Seahorse XFp Cell Mito Stress Test Kit and XF glycolysis stress test kit (Agilent Technologies). OCR and ECAR were measured under basal conditions and in response to several metabolic drugs provided in the kit ...
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bioRxiv - Cell Biology 2024Quote: ... libraries were quantified using a Qubit dsDNA HS DNA Kit (Thermo Fischer Scientific) and visualized on an Agilent 2100 Bioanalyzer using an Agilent High Sensitivity DNA Kit (Agilent Technologies). Libraries were sequenced in a HiSeq2500 (Illumina Inc. ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial respiration and glycolysis were determined by measuring OCRs and ECARs in the extracellular space with a Seahorse XF96 Extracellular Flux Analyzer with the XF Cell Mito Stress Test Kit and XF Glycolysis Stress Test Kit (Seahorse Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: Glycolysis and Mitochondrial stress tests were performed on confluent HUVECs according to the manufacturer’s kits and protocols (Agilent Seahorse XF glycolysis stress test kit, Agilent Technologies, Inc.). 1 μM Oligomycin was used ...
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bioRxiv - Cancer Biology 2024Quote: ... the quality of the libraries was assessed using Agilent High Sensitivity DNA Kit and Agilent Bioanalyzer 2100 DNA Kit (Agilent Technologies). Libraries were sequenced on an Illumina NovaSeq 6000 with the sequencing configurations recommended by 10X Genomics ...
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bioRxiv - Pathology 2020Quote: ... 50 slides were stained with PD-L1 IHC 22C3 pharmDx assay according to a standard staining protocol using appropriate automated staining devices (Dako Autostainer Link 48 platform). 65 slides were stained with anti-human PD-L1 rabbit monoclonal antibody (clone SP142 ...
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bioRxiv - Microbiology 2021Quote: ... Purified cDNA libraries were qualified on an Agilent Technologies 2200 TapeStation using a D1000 ScreenTape assay (Agilent Cat#5067-5582 and Cat#5067-5583). The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Kapa Biosystems Cat#KK4824).
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bioRxiv - Microbiology 2021Quote: ... and the molecular weight was assessed using the Agilent TapeStation 4200 and the Agilent Genomic DNA ScreenTape Assay (Agilent; 5067-5365 and 5067-5366). A sequencing library was prepared using the Genomic DNA by Ligation Kit (Oxford Nanopore Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR was performed using a SYBR green assay system with Phusion® High-Fidelity DNA Polymerase using a Stratagene Mx3000 qPCR machine (Agilent Technologies, CA, USA). The mRNA levels were normalized to beta-actin abundance ...
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bioRxiv - Microbiology 2022Quote: ... The amount and integrity of RNA were assessed on a Bioanalyzer RNA 2100 Nano/Pico Chip with Total RNA Assay (Agilent, Santa Clara, CA, USA). To ensure that RNA was DNA-free ...
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bioRxiv - Cell Biology 2022Quote: ... The quality and size distribution of the amplified libraries were determined by chip-based capillary electrophoresis on Agilent 2100 Bioanalyzer High Sensitivity DNA assays (Agilent Technologies; Part # 5067-4626). Libraries were quantified using the Takara Library Quantification Kit (Part # 638324) ...
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bioRxiv - Cell Biology 2022Quote: ... T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM; 103575-100) supplemented with 4mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: Naive CD3+ T cells were placed on PLL-coated Seahorse Bioanalyzer XFp culture plates (3 × 105 cells/well) with Seahorse XF RPMI Assay Media (RPMI Medium, pH 7.4, 103576-100; Agilent Technologies, Santa Clara, CA, USA), supplemented with 10 mM glucose (103577-100 ...
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bioRxiv - Cell Biology 2022Quote: ... measurement before being assessed for quality by chip-based capillary electrophoresis using Agilent 2100 Bioanalyzer RNA 6000 Pico assays (Agilent Technologies; Part # 5067-1513).
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bioRxiv - Cancer Biology 2023Quote: ... that were freshly obtained prior to start of pembrolizumab (n=32) using the companion diagnostic assay of pembrolizumab (PD-L1 IHC 22C3 pharmDx, Agilent Technologies, Carpinteria, CA, USA). When no fresh tumor biopsy was available ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were validated for length and adapter dimer removal using the Agilent TapeStation 4200 D1000 High Sensitivity assay (Agilent Technologies, Santa Clara, CA, USA) then quantified and normalized using the dsDNA High Sensitivity Assay for Qubit 3.0 (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... and fragment length profiles were assessed using the Agilent 4200 TapeStation and Agilent High Sensitivity D5000 ScreenTape Assay (Agilent; 5067-5592 and 5067-5593).
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bioRxiv - Immunology 2022Quote: Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) were measured using Seahorse XF Glycolysis Rate Assay on Seahorse XFp Flux Analyzer (Agilent Technologies, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Libraries were validated for length and adapter dimer removal using the Agilent TapeStation 4200 D1000 High Sensitivity assay (Agilent Technologies, Santa Clara, CA, USA) then quantified and normalized using the dsDNA High Sensitivity Assay for Qubit 3.0 (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and the amplicon size and DNA concentration were checked with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies, Inc, CA, USA). Sequencing libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit and the samples were sequenced with Illumina NovaSeq 6000 platform using SP flowcell (PE250).
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bioRxiv - Cancer Biology 2024Quote: ... Library concentration and average size was verified using the TapeStation DNA 5000 Assay Reagents and Screen Tapes (Agilent, Cat. #5067-5589 and #5067-5588). Libraries were normalized and pooled based on the TapeStation concentrations ...
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bioRxiv - Genomics 2021Quote: ... we performed another secondary analysis between two groups of control samples (Leicester study controls vs. Ottawa and ATVB controls) representing two main enrichment kits (Illumina ICE vs. Agilent SureSelect kits). The statistical analysis was performed in R 3.3.3 (55) ...
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bioRxiv - Synthetic Biology 2021Quote: ... using the GeneMorph II random mutagenesis kit (Agilent, USA). The generated PCR products were inserted back into the respective backbones using Gibson Assembly ...
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bioRxiv - Cancer Biology 2021Quote: ... and XF Cell Mito Stress Test Kit (Seahorse Bioscience). Cells were seeded at 50,000 cells/well (~80-90% confluent when assayed ...
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bioRxiv - Cancer Biology 2021Quote: ... The XF Cell Mito Stress Test Kit (Agilent, 103015), was used for the assay ...
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bioRxiv - Cell Biology 2020Quote: ... ookinete and sporozoites using an RNA purification kit (Stratagene). cDNA was synthesised using an RNA-to-cDNA kit (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) for qualitative analysis ...
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bioRxiv - Genetics 2021Quote: ... and subcloned using Strataclone PCR Cloning Kit (Agilent 240205). Either Donorguide or ssODN injected zebrafish were combined ...