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Citations for Agilent :
1751 - 1800 of 1929 citations for Recombinant Rabbit CXCL12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: The EnVision™ staining kit (G|2 Double-stain System, Rabbit/Mouse, DAB+/Permanent RED code K5361; Agilent technologies, Dako DK) was used for the immunohistochemical stain of Mamu-DR ...
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bioRxiv - Neuroscience 2021Quote: The EnVision™ staining kit (G|2 Double-stain System, Rabbit/Mouse, DAB+/Permanent RED code K5361; Agilent technologies, Dako DK) was used for the immunohistochemical stain of Mamu-DR ...
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bioRxiv - Neuroscience 2021Quote: The optimal concentration was determined for each antibody: CD3 (polyclonal rabbit – anti-human CD3 IgG, cat. no. A045201-2, Agilent Technologies), 1:60 ...
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bioRxiv - Cell Biology 2020Quote: ... and the following HRP conjugated secondary antibodies: swine anti-rabbit (1:3000 for embryo levels, 1:10000 for in vitro assays; Dako, P0399) and sheep ECL anti-mouse (1:3000 ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated for 45 minutes in 1% dried skim milk in PBS-T with rabbit anti-human C1q (1:300, Dako, A0136) and HRP-conjugated goat anti-rabbit IgG (1:10.000 ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated antibodies were used as secondary antibodies (Swine anti-rabbit 1:3000, Goat anti-mouse 1:5000, Dako, Glostrup, Denmark). Membranes were developed with Clarity Western ECL Substrate 1:1 (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... The secondary antibodies were horseradish peroxidase- conjugated goat anti-rabbit IgG antibodies (Nichirei, 424141) for HRP-IHC and visualized using a Liquid DAB+ Substrate Chromogen System (DAKO, K3468), or Alexa Fluor Plus 488- or 555-conjugated goat anti-mouse ...
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bioRxiv - Microbiology 2022Quote: ... For visualising HSV-1 plaques mouse anti-gD (LP2) 1:50 [75] and HRP-conjugated rabbit anti-mouse 1:5000 (DaKo, P0161) were used.
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently rinsed with PBS supplemented with 0.01% Tween 20 and probed with horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (1 in 500 dilution) (DAKO, Agilent Technologies) for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... Slides were then washed in PBS/0.05% Tween 20 and incubated with rabbit anti human VWF (A008229-2, DAKO/Agilent tech) 1/500 or rabbit IgG isotype control (AB-105-C ...
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bioRxiv - Neuroscience 2022Quote: A one-in-six series of coronal brain sections from each mouse were stained on slides using a modified immunofluorescence protocol22 for the following antibodies - astrocytes (rabbit anti-GFAP, 1:1000, Agilent Z0334), microglia (rat anti-mouse CD68 ...
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bioRxiv - Neuroscience 2023Quote: ... detected with polymeric alkaline phosphatase conjugated anti-rabbit IgG (BrightVision DPVR-AP, Immunologic) and visualized with stay red (DAKO, Glostrup, Denmark). Next ...
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bioRxiv - Cell Biology 2023Quote: ... frozen femur sections were blocked with 3% BSA and 5% goat serum in PBS, incubated with Cgrp antibody (rabbit polyclonal, abcam #ab47027, 1:300 in DAKO antibody diluent (Agilent, #S0809)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed a further three times with TBS-T for 5 minutes and stained with DAKO EnVision-HRP rabbit/mouse (Agilent; #K5007) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and incubated with HRP-conjugated secondary antibodies for 1 h at RT (Goat-anti-Rabbit-HRP, Goat-anti-Mouse-HRP, both Dako Denmark). Marker lanes were incubated with Streptavidin-biotinylated-HRP (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with anti-rabbit or anti-mouse horseradish peroxidase- labelled secondary antibody (Dako EnVision+ System- HRP Labelled Polymer) followed by a chemiluminescent reaction (Clarity Western ECL Substrate ...
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bioRxiv - Neuroscience 2023Quote: Anti-PrP monoclonal antibody ICSM35 (D-Gen Ltd) was used with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Cell Biology 2023Quote: ... solution (pH 7.4) and incubated with goat anti-mouse or anti-rabbit immunoglobulin labeled with dextran molecules and horseradish peroxidase (EnVision, Dako Omnis) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed in TBS-t and subsequently incubated with goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody (0.5 µg/ml; Dako, VIC, Australia) or rabbit anti-Goat IgG horseradish peroxidase-conjugated secondary antibody (0.125 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 min) before incubation with either rabbit anti-mouse IgG (1.3 µg ml−1, 1% BSA in PBST; Agilent, P026002-2), goat anti-rabbit IgG (250 ng ml−1 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated with primary antibodies (chicken anti-Map2 [Thermo Scientific, PA1-16751, 1:1000] and rabbit anti-Gfap [Dako, Z0334 ...
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bioRxiv - Biochemistry 2023Quote: ... blots were incubated for 1h at room temperature with a horse radish peroxidase-labeled secondary antibody (anti-rabbit, Dako; 1/1000), and revealed by ECL chemiluminescence (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... washed in TBST and incubated for 1-2 h with the peroxidase-coupled secondary antibody (HRP-coupled anti-rabbit IgG (Dako, #P0448), HRP-coupled anti-mouse IgG (Dako ...
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bioRxiv - Bioengineering 2024Quote: ... IHC antibodies: rabbit anti-EGFR (clone SP84, Cell Marque #14R-15) at 1:25 and mouse anti-Ki67 (clone MIB1, Dako #M7240) at 1:50 with ME Kit ...
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bioRxiv - Immunology 2024Quote: ... The membrane was washed three times in block buffer followed by a 1 h incubation with anti-goat/-rabbit HRP-conjugated secondary antibodies (1:5000, Agilent Dako) at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in primary antibody (anti-Alk1 Rabbit Abcam #ab68703, 1:100; anti-CD31 Mouse Dako #M0823, 1:500) over-night at 4°C ...
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bioRxiv - Immunology 2024Quote: A total of 10 µg/ml rHA were coated on an ELISA plate at 4 °C ON and afterwards incubated with rabbit anti-HA antibody (1:2000 dilution) (Dako, A0001) 2h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed 3 times for 10 minutes each with TBST and probed with appropriate HRP secondary antibody (anti-Rabbit Immunoglobulin/HRP, Dako P044801 or anti-mouse Immunoglobulin/HRP, Dako P0260) at 1:1000 in TBST with 5% skim milk ...
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bioRxiv - Bioengineering 2021Quote: ... 10-20 µl injection volume depending on protein concentration) were injected on a Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) at a flow rate of 100 µl/min using solvent A (0.1% formic acid and 0.05% trifluoroacetic acid in water ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Molecular Biology 2020Quote: ... All samples were depleted of the 7 most abundant proteins with the multiple affinity removal system (MARS Hu7 spin cartridge) according to the manufacturers protocol (Agilent Technologies). Samples were further reduced using 4 mM DTT ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Primary anti-CD31 antibody in Tris-HCl buffer containing stabilizing protein and 0.015 mol/L sodium azide (Dako Antibody Diluent, Dako, Glostrup, Denmark) were then added to the slides ...
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bioRxiv - Microbiology 2019Quote: ... MBP-fusion proteins were purified with amylose resin following the manufacturer’s instructions (GE Healthcare, Pierce, Agilent Technologies and New England Biolabs). MBP-fusions (1 µg each in 100 µl of 1% NP40 ...
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bioRxiv - Cell Biology 2020Quote: Zyxin and Pxl1 LCR proteins were expressed in Escherichia coli strain BL21-Codon Plus (DE3)-RP (Agilent Technologies, Santa Clara, CA) with 0.5 mM isopropyl β-D-1-thiogalactopyranoside for 16 h at 16 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were prepared according to the instruction of the Agilent protein 80 kit and analyzed on a 2100 Bio-analyzer device (Agilent technologies). The ATPase activity of FSS-PscN was determined by measuring the release of total phosphate at 37°C for 30 minutes which was detected by the malachite green method (Casabona et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... approximately 8 µg of protein was injected on a Zorbax 300SB-C18 column (5 µm, 300Å, 1×250mm IDxL; Agilent Technologies) and separated using a 30 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Immunology 2020Quote: ... followed by a quick rinse in TBS before being returned to the black chamber to be incubated with serum free protein block (Dako X0909) for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of 2 – 4 mg/ml protein samples were applied to a column using the 1260 Infinity HPLC system (Agilent Technologies) coupled to a MiniDawn Treos detector (Wyatt Technologies ...
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bioRxiv - Immunology 2022Quote: ... brucei Heat Shock Protein 70 (TbHSP70) (Kind gift from Professor Keith Matthews, Edinburgh, UK) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s haematoxylin.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected using tryptophan fluorescence (λex=280 nm; λem=315 nm) using a fluorescence detector (Agilent technologies 1200 series, G1321A). The peak heights of the monomeric hsFPN peaks were used to assess the stability by normalizing heights to the corresponding value from samples incubated at 4 °C (100% stability) ...
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bioRxiv - Cancer Biology 2020Quote: ... the serum samples were immunoaffinity depleted of the 14 most abundant proteins using IgY14 LC20 (cat No. 5188-6557, Agilent, USA). The depletion was performed on an Agilent 1260 HPLC (1260 Infinity ...
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Biochemistry 2021Quote: Serum samples were depleted of the top 14 interfering high-abundant proteins using the Multiple Affinity Removal Column Human 14 (Agilent, USA). Then ...
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bioRxiv - Immunology 2023Quote: ... brucei parasites using a polyclonal rabbit antibody raised against T. brucei luminal binding protein 1 (BiP) (J. Bangs, SUNY, USA) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s Haematoxylin ...
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bioRxiv - Molecular Biology 2023Quote: The above 12 OR genes linked to a wild-type internal ribosome entry site from the encephalomyocarditis virus (EMCV IRES) and the mCherry fluorescent protein gene (Shaner et al., 2004) were inserted into a modified pBlueScript KS (Agilent/Stratagene) called plasmid “L” (Zboray et al. ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies). Lager scale transient transfections were purified on the Akta FPLC system using 1mL HiTrap MabSelect PrismA™ affinity chromatography resin (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: ... approximately 2 µg of protein was injected on a Zorbax Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) and separated using a 15 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescence spectroscopy measurements of 2 μM of each protein sample were obtained using the Varian Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies) with an excitation wavelength of 280 nm and recording the emission spectra between 300 nm and 400 nm.