Labshake search
Citations for Agilent :
1401 - 1450 of 1929 citations for Recombinant Rabbit CXCL12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... x1000 dilution) and secondary polyclonal swine anti-rabbit antibody conjugated to HRP (P0399, Agilent Technologies, x3000 dilution) were used for the detection of HAUS subunits ...
-
bioRxiv - Biochemistry 2024Quote: ... ab109401 (1:5000)) or and anti-total Tau antibody (rabbit anti-human Tau, Dako, #A0024 (1:5,000)) diluted in 3% BSA in PBS-T overnight at 4°C shaking ...
-
bioRxiv - Plant Biology 2024Quote: ... Conjugated anti-rabbit and anti-mouse immunoglobulins were used as the secondary antibodies (Agilent Dako, 1:20000). Immunodetection was performed using an ECL Plus Western Detection Kit and revealed with an ImageQuant LAS 4000 mini CCD camera system (GE Healthcare) ...
-
bioRxiv - Plant Biology 2024Quote: ... Conjugated anti-rabbit and anti-mouse immunoglobulins were used as the secondary antibodies (Agilent Dako, 1:20000). Immunodetection was performed using an ECL Plus Western Detection Kit and revealed with an ImageQuant LAS 4000 mini CCD camera system (GE Healthcare) ...
-
bioRxiv - Cell Biology 2023Quote: ... The membranes were further incubated with a goat anti-rabbit HRP-conjugated secondary antibody (1:2000, DAKO) for 1 h at room temperature ...
-
bioRxiv - Genomics 2021Quote: ... using a mixture of equal volume of ready-to-use protein block serum-free solution (Agilent Dako, cat.no ...
-
bioRxiv - Cancer Biology 2021Quote: ... were immobilized on each Protein-A cartridge (25 µL bed volume, Agilent Technologies, cat. no. G5496-60018) and crosslinked ...
-
bioRxiv - Cell Biology 2020Quote: ... Coverslips were rinsed in PBS three times and incubated in serum free DAKO Protein Block (X0909, DAKO) for 1h at RT ...
-
bioRxiv - Immunology 2021Quote: ... and capillary electrophoresis sodium dodecyl sulphate (CE-SDS; protein 230, BioAnalyzer 2100, Agilent, Santa Clara, CA, USA).
-
bioRxiv - Cancer Biology 2019Quote: ... The endogenous peroxidase was blocked with a commercial solution (Protein Block, Agilent Technologies, Santa Clara, CA, USA), and the samples were incubated with primary antibodies overnight at 4ºC ...
-
bioRxiv - Biochemistry 2020Quote: Skd3 variants were expressed as an N-terminally MBP-tagged protein in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in 40mM HEPES-KOH pH=7.4 ...
-
bioRxiv - Cancer Biology 2020Quote: ... After blocking nonspecific-binding sites on sections with Protein Block Serum-Free reagent (Dako North America Inc.) for 30 minutes ...
-
bioRxiv - Synthetic Biology 2021Quote: Scanning mutant libraries were constructed using saturation mutagenesis using the QuikChange HT Protein Engineering System from Agilent following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... After blocking nonspecific-binding sites on sections with Protein Block Serum-Free reagent (Dako North America Inc.) for 30 minutes ...
-
bioRxiv - Immunology 2021Quote: ... FcR binding was quantified by incubating immune complexes with biotinylated FcRs (FcγR2A-1, FcγR2A-2, FcγR3A, courtesy of Duke Protein Production Facility) conjugated to Steptavidin-PE (Prozyme). Flow cytometry was performed with an IQue (Intellicyt ...
-
bioRxiv - Cell Biology 2022Quote: ... cells or tissue sections were incubated with Protein Block Serum-Free Ready-To-Use (Dako, #X 0909) for 30 min at room temperature ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein in the eluate was detected with a UV detector at 280 nm (Agilent Technology 1260 UV), a quasi-elastic light scattering (QELS ...
-
bioRxiv - Cell Biology 2021Quote: Constructs encoding the 6His-SUMO tagged proteins were transformed into chemically competent BL21(DE3) Escherichia coli (Agilent). A fresh bacteria colony was inoculated to 10 ml LB broth containing 200 μg/ml Ampicillin and 50 μg/ml Chloramphenicol and grown at 37°C overnight ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and slides were incubated with Dako Serum Free Protein Block (#X090930-2, Agilent, Santa Clara, CA, USA) at room temperature ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins were diluted and scanned in a quartz cuvette with a Cary 60 UV-Vis spectrophotometer (Agilent). Extinction coefficients for Cas9 ...
-
bioRxiv - Biochemistry 2020Quote: ... All E protein variants were obtained by site-directed mutagenesis using QuikChange kit (Stratagene, La Jolla, California) and were confirmed by sequencing the plasmid DNA at Macrogen Company (Seoul ...
-
bioRxiv - Cell Biology 2021Quote: ... A53T and E46K α-synuclein proteins were expressed in BL2-Gold(DE3) competent cells (Agilent, Cat #230132). Protein expression was induced at log phase with 200 μM IPTG for 16 hours at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The immunoprecipitated proteins were analyzed using a precision HPLC unit (1100 series, Agilent, Santa Clara, CA, USA) equipped with a reverse-phase column and a micro-analytical UV detector system (SG Highteco ...
-
bioRxiv - Microbiology 2021Quote: ... 50 μl of protein (10 mg/ml) were injected into a size-exclusion column (Agilent BioSec-3) equilibrated in 50 mM Tris ...
-
bioRxiv - Molecular Biology 2021Quote: Protein expression was conducted in E.coli BL21 (DE3) Codon Plus cells (Agilent Technologies, San Diego, CA, USA). Induced cultures were grown at 18° C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... 600 ng of total protein was injected onto a home-packed PLRP column (PLRP-S) (Agilent Technologies), 10-µm particle size ...
-
bioRxiv - Biochemistry 2023Quote: The EQ2 mutations were introduced sequentially into each protein using QuikChange II Site-Directed Mutagenesis (Agilent Technologies) with all growth media supplemented with 0.4% (w/v ...
-
bioRxiv - Cancer Biology 2023Quote: ... The sections were rinsed in phosphate buffered saline (PBS) and blocked in Protein-Block buffer (DakoCytomation #0909) for 15 min ...
-
bioRxiv - Biochemistry 2023Quote: ... The maltose-binding protein (MAL) gene from Escherichia coli (gene identifier 7129408) in a pBlueScript KS (Stratagene) was amplified by PCR using T7 minimal promoter primers under the following conditions ...
-
bioRxiv - Immunology 2023Quote: ... proteins were extracted from the cytosol fraction by incubation with 1% (v/v) StrataClean resin (Agilent Technologies) overnight at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... Proteins were extracted from sucrose gradient fractions by incubation with 1% (v/v) StrataClean resin (Agilent Technologies) overnight at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... metabolic labelling was performed in BOEC protein extracts using ENG mAb SN6h (Dako Denmark A/S, Denmark). Modifying methods of Pece et al,18 a 1 hour “pulse” with 50μCi/ml of 35S methionine ...
-
bioRxiv - Cell Biology 2023Quote: ... The results were subsequently introduced into the database for protein identification searches using Spectrum Mill (Agilent Technologies). All searches were carried out with “Drosophila melanogaster” as taxonomy in NCBInr database and 0.5 Da of tolerance on MS/MS fragments ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were analyzed by SDS-PAGE followed by immunoblotting with rabbit peroxidase-anti-peroxidase (PAP) antibodies (Z0113, Dako) or rabbit anti-GFP (ab6556 ...
-
bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for immunoblotting were Horseradish Peroxidase (HRP)-conjugated swine anti-rabbit (DAKO, cat# P0399; 1:4,000) or goat anti-mouse (DAKO ...
-
bioRxiv - Cancer Biology 2020Quote: ... Primary antibody was detected after probing for 1 hour with HRP-linked rabbit anti-mouse IgG (P0161, Dako) or goat anti-rabbit IgG (P0448 ...
-
bioRxiv - Neuroscience 2019Quote: Antibody specificity was tested using a negative staining procedure with normal rabbit IgG (1:250; Dako, Glostrup, Denmark) instead of the primary antibodies ...
-
bioRxiv - Pathology 2020Quote: ... used at a dilution of 1:50 and a FITC-conjugated rabbit polyclonal antibody to IgA (Dako, F0204). The polyclonal sheep anti-nephrin antibody was also directly conjugated to Alexa-488 using NHS chemistry ...
-
bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies were detected using a standard anti-rabbit secondary antibody followed by 3,3′-diaminobenzidine (DAB) revelation (Dako). Quantification of immunohistochemical signal intensity was performed by color deconvolution using ImageJ (Fiji version ...
-
bioRxiv - Microbiology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse (P0399) or swine anti-rabbit secondary antibodies (P0447) were from Dako.
-
bioRxiv - Microbiology 2020Quote: ... Polyclonal goat anti-mouse IgG/HRP and Polyclonal goat anti-rabbit IgG/HRP (1:2000) were from Dako, Denmark ...
-
bioRxiv - Cell Biology 2020Quote: ... The immune complexes were visualized with anti-rabbit or anti-mouse HPR-conjugated secondary antibodies (DAKO A/S) and enhanced by Clarity Western ECL Substrate (BioRad ...
-
bioRxiv - Cell Biology 2021Quote: ... Anti-Spike antibody binding was detected using HRP-conjugated anti-rabbit secondary antibodies (1 in 7000 dilution) (Dako) and visualized using ECL (KPL ...
-
bioRxiv - Cancer Biology 2022Quote: ... Primary antibody was detected after probing for 1 hour with HRP-linked rabbit anti-mouse IgG (P0161, Dako) or goat anti-rabbit IgG (P0448 ...
-
bioRxiv - Cancer Biology 2022Quote: ... the slices were incubated with primary antibodies: rabbit polyclonal anti-PSMA/GCPII (1:150) (M362029-2; DAKO, US), anti-CD68 (1:250 ...
-
bioRxiv - Microbiology 2022Quote: ... sections were incubated with peroxidase labeled goat-anti-Rabbit IgG (1:100) (P0448, DAKO, Agilent Technologies Netherlands B.V) in PBS/0,1% BSA for 1h at RT ...
-
bioRxiv - Microbiology 2022Quote: ... sections were incubated with peroxidase labeled goat-anti-Rabbit IgG (1:100) (P0448, DAKO, Agilent Technologies Netherlands B.V) in PBS/0,1% BSA for 1h at RT ...
-
bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the secondary antibody rabbit anti-mouse conjugated to horseradish peroxidase (Dako, 1:2000) for 90 min at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... the slices were stained with primary antibodies for GFAP (polyclonal rabbit anti-cow GFAP, 1:500; Z0334, Dako) and NeuN (monoclonal mouse anti NeuN ...
-
bioRxiv - Cell Biology 2022Quote: ... Blots were then washed with PBS-T and incubated with either swine anti-rabbit HRP (1:3000, Dako) or goat anti-mouse HRP (1:3000 ...