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Citations for Agilent :
1751 - 1800 of 4255 citations for 6H 1 2 Thiazine 6 ethoxy 5 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The dried labelled peptide plexed sample was then resuspended in 400uL of 1 M TEAB and fractionated using an Agilent 1100 HPLC system and high pH reversed phase chromatography on a Zorbax Eclipse XDB-C18 column (4.6 x 150mm, 5-micron) from Agilent (Santa Clara, CA). Sixty fractions were collected at 0.5 mL/min over 105 min using a gradient of 0-5% solvent B in 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Blocking with endogenous peroxidase was performed with Protein Block (Novocastra Laboratories) for 5 min and subsequent steps were performed in DAKO autostainer link 48 (Agilent Technologies, Inc.). Slides were counterstained in hematoxylin-eosin ...
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bioRxiv - Immunology 2023Quote: ... Bacteria were fixed with 0.5% formaldehyde at RT for 30 min and then wells were washed twice with a washer dispenser (BioTek EL406, Agilent Technologies, US) with PBS ...
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bioRxiv - Bioengineering 2023Quote: ... were obtained by high pressure liquid chromatography on an Agilent Bio SEC-5 500 Å (for KWOCA assemblies) or 1000 Å column (for I32-10) (Agilent) at a flow rate of 0.35 mL/min by injection of 10 μL of purified eluate using a mobile phase of Tris-buffered saline (50 mM Tris pH 8 ...
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bioRxiv - Neuroscience 2024Quote: ... The gas chromatograph was equipped with a 5 m guard column + 30 m (I.D. 250 μm, film 0.25 μm) DB-35MS capillary column (Agilent J&W GC Column). Helium was used as the carrier gas with a constant flow rate of 1.2 mL/min.
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bioRxiv - Plant Biology 2024Quote: ... CMV genomic RNAs (strain Fny) were synthesized with 5’ monomethylated cap analog by T7 RNA Polymerase (Agilent Technologies, Santa Clara, CA, #600123) from PCR product templates (see Supplementary Table S1 for primer sequences) ...
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bioRxiv - Bioengineering 2024Quote: ... and reverse primer (5’-AGGTCATGTACTGGGCATAAT-3’) binding to the GFP sequence with 5 µL of Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies, USA) and 0.15 µL of 2 µM reference dye.
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bioRxiv - Cell Biology 2020Quote: ... followed by 1-hour incubation with peroxidase-labeled goat anti-mouse antibodies (1:1000; DAKO) and a 7 min incubation with the True Blue™ (KPL ...
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bioRxiv - Microbiology 2020Quote: ... or chicken anti-GFAP (Abcam, 1:500, or mouse anti-CD68 (Agilent/Dako, 1:200) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... or chicken anti-GFAP (Abcam, 1:500, or mouse anti-CD68 (Agilent/Dako, 1:200) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Antibodies for labeling were: anti-GFP (A11122, Thermo, 1:250) anti-vimentin (Dako, 1:1000), peanut agglutinin (PNA ...
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bioRxiv - Developmental Biology 2024Quote: ... insulin (1:100; custom made by Cambridge Research Biochemicals) and somatostatin 1:300 (DAKO-A0566). Liver and insulin area was calculated on maximum projections using the Fiji threshold function.
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bioRxiv - Physiology 2023Quote: ... freshly prepared) was flowed at 1 ml/min through a 1:100 flow splitter (Agilent Technologies 1260 Infinity II ...
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bioRxiv - Cell Biology 2023Quote: ... freshly prepared) was flowed at 1 ml/min through a 1:100 flow splitter (Agilent Technologies 1260 Infinity II ...
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bioRxiv - Immunology 2023Quote: ... EPCAM (CST, 1:5000), Claudin1 (SantaCruz, 1:200) and the bound secondary antibody – peridoxidase (DAKO) was detected with Liquid DAB + substrate chromogen system (DAKO ...
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bioRxiv - Microbiology 2020Quote: FITC-conjugated rabbit F(ab’)2 anti-human C1q was obtained by digestion of FITC-conjugated rabbit anti-human C1q (Dako) using 1 U/ µg of recombinant His-tagged IdeS protease ...
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bioRxiv - Cancer Biology 2021Quote: ... 2×105 cells/well were plated in five technical replicates on collagen-coated XF96 polystyrene cell culture microplates (Seahorse, Agilent) in GM ...
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of each purified final library was run on an Agilent TapeStation HS D1000 ScreenTape (Agilent Technologies, 5067-5584). The libraries were quantified using the Quant-iT 1X dsDNA HS kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the cells were resuspended in 500 µl PBSF buffer (PBS+2% FBS) and analyzed using ACEA NovoCyte TM (Agilent Biosciences); non-transfected 293T cells served as a negative control.
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bioRxiv - Cell Biology 2022Quote: ... Before separation peptides were first trapped (Dr Maisch Reprosil C18, 3 μm, 2 cm x 100 μm) and then separated on an analytical column (Agilent Poroshell EC-C18 ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of labeled cRNA was hybridized with a human gene expression microarray 60K (Agilent Technologies, Santa Clara CA, USA). The microarray workflow quality control was implemented using the Agilent Spike-In Kit which consisted of a set of 10 positive control transcripts optimized to anneal to complementary probes on the microarray with minimal self-hybridization or cross-hybridization ...
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bioRxiv - Physiology 2019Quote: ... and analyzed by Western blotting using the specific first antibodies listed in Supplementary Table 2 with goat anti-rabbit and goat anti-mouse peroxidase-conjugated immunoglobulin as secondary antibodies (Dako). The blots were then processed with the luminescence technique ECL (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... D614G and other SARS-CoV-2 Spike single mutations were generated using the QuickChange II XL site-directed mutagenesis protocol (Stratagene) and the pCG1-SARS-CoV-2-S plasmid kindly provided by Stefan Pöhlmann ...
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bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
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bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
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bioRxiv - Cell Biology 2021Quote: RNAi-resistant EGFP-ORP5A and EGFP-ORP5B were generated by introducing 4 silent point mutations in the region targeted by the 2 siRNA oligos (#10 and #11) by site-directed mutagenesis (Quickchange II-XL, Stratagene) and the following primers:
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bioRxiv - Biochemistry 2020Quote: ... Radioactivity was detected using an in-line Lablogic Radiodetector and the analysis was calibrated using a co-injected 2-aminobenzamide-labeled dextran ladder (ProZyme/Agilent) detected on a Dionex fluorescence detector.
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bioRxiv - Plant Biology 2021Quote: ... The SEGS-2 ATG mutant (pNSB2110) was created in S2-1.5b (pNSB1869) using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and the primers ...
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bioRxiv - Plant Biology 2021Quote: ... pGEX6P1:SYT1C2B (residues G374-end) and pGEX6P1:SYT1-SMPC2A (residues N34-E397) were transformed into Rosetta 2 (DE3) pLysS cells (Stratagene) for protein expression using standard protocols ...
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bioRxiv - Microbiology 2021Quote: ... 2□μl of each sample was used to assess RNA quality using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA), and the RNA integration numbers (RIN ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation and the combination of mutations found in SARS-CoV-2 variants were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana vesicular stomatitis virus (VSV ...
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bioRxiv - Physiology 2021Quote: ... Peptides were first trapped (Dr. Maisch Reprosil C18, 3 μm, 2 cm × 100 μm) prior to separation on an analytical column (Agilent Poroshell EC-C18 ...
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bioRxiv - Microbiology 2020Quote: ... Equal number of live cells was seeded at 2*105 cells per well in XF96 V3 PS plates (Seahorse Bioscience) precoated with 0.5 mg/ml Corning® Cell-Tack™ Cell and Tissue Adhesive (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... A Ddyo7-mCherry expression plasmid was generated by first TA cloning a PCR product (myo42 to myi185+2) encompassing the myoi 3’ region of the gene (aa 475 - end) minus the stop codon using StrataClone (Agilent) (pDTi289+2) ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were then washed twice with TBS-T followed by incubation for 1 h at room temperature in immunoreaction enhancer solution 2 (Can Get Signal; TOYOBO) with peroxidase-conjugated antibody against rabbit IgG (Dako) as the secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Cellular oxygen consumption was assessed in basal condition (prior to any addition) and after addition of oligomycin (2 μM; Agilent) Carbonyl cyanide-4 (trifluoromethoxy ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2 hours at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope.
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bioRxiv - Microbiology 2022Quote: ... ORF6 mutations were introduced into the pLVX-StrepII-SARS-CoV-2-ORF6-IRES-Puro and pLVX-EF1α-SARS-CoV-2-ORF6-2xStrep-IRES-Puro plasmids using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions using SDM primers listed below ...
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bioRxiv - Biophysics 2022Quote: ... was pre-equilibrated with 10 mM pH 7 sodium phosphates with 150 mM NaCl on an Infinity 2 1260 HPLC system (Agilent) with an inline degasser ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were filtered through Agilent Captiva syringe filters in to deactivated glass inserts (0.2 mL) placed inside amber glass vials (2 mL; Agilent Technologies). LC separations were carried out using a 1200 series LC system (Agilent Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... Residual CP and its metabolite 3,5,6-trichloro-2-pyridino (TCP) was quantified using a reverse-phase C18 column (ZORBAX Eclipse Plus, Agilent Technologies, USA) fitted with Agilent Technologies 1260 Infinity HPLC system equipped with a binary pump ...
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bioRxiv - Biochemistry 2022Quote: ... CaCl2 was added to a final concentration of 2 mM and the supernatant was added to a disposable column containing 400 μL calmodulin resin (Agilent) pre-equilibrated with E buffer and 2 mM CaCl2 ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 nucleocapsid (EY-2A, a kind gift from Prof Alain Townsend) primary antibodies and appropriate HRP-conjugated secondary antibodies (DAKO). Chemiluminescence substrate (West Dura ...
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bioRxiv - Microbiology 2020Quote: ... DNA removal was confirmed by PCR using primers OL398 and OL399 (Table 2) and RNA quality was assessed using an Agilent 2100 Bioanalyzer system with corresponding RNA 6000 Nano kit (Agilent) to confirm RNA integrity (RIN) ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting DNA fragments were cloned in frame with the FLAG tag using the oligonucleotides that are indicated in Supplementary file 2 into the pESC-URA vector (Agilent), in which the GAL10 and GAL1 promoters were replaced with the Cup1 promoter ...
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bioRxiv - Microbiology 2021Quote: Headspace CO concentrations were monitored every 2-4 days using an Agilent 6890N gas chromatograph (GC; Agilent Technologies, California, US) equipped with a nickel catalyst methaniser (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... viral protein in the virus-infected cells was detected by ELISA assay using anti-SARS-CoV-2 nucleocapsid mAb (40143-R001, SinoBiological) and HRP-conjugated goat anti-rabbit pAb (P0448, Dako). After 10 min incubation with TMB substrate ...