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Citations for Agilent :
1701 - 1750 of 2281 citations for Mono 2 Ethyl 5 Oxohexyl Phthalate 13C4 99% Dehp Metabolite Vi 100Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and 1 mL of the supernatant was analyzed with a spectrophotometer (CARY-60, Agilent) at 565 ...
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bioRxiv - Plant Biology 2020Quote: ... The excised fragment was introduced in the MCS 2 (EcoRI/NotI) of the yeast expression vector pESC-URA (Stratagene), cloned in the E ...
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bioRxiv - Bioengineering 2022Quote: ... SARS-CoV-2 RBD mutant plasmids were generated by Quikchange site-directed mutagenesis according to manufacturer’s instructions (Agilent, 210513). Biotinylated proteins were made by co-transfecting Avitagged RBD plasmids with a BirA expression plasmid and into Expi293 cells using FectoPRO ...
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bioRxiv - Microbiology 2019Quote: ... Immunolabelling was performed as follows: grids were placed directly on drops of 2% normal goat serum (DAKO, Glostrup, Denmark) in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... Each sample set was run together with the external standard (2-AA labeled dextran ladder, Glyko Prozyme, Hayward, CA). In each chromatogram 15 individual peaks were identified and peak assignments were made basing on glucose units (GU) ...
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bioRxiv - Bioengineering 2021Quote: ... samples were subjected to a treatment with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909 ...
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bioRxiv - Genetics 2020Quote: ... Step 1.3 of the protocol was performed with the following modifications: Step 1.3.q slides were incubated in Dako bluing buffer (#CS70230-2 Agilent) for 30 s ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and 10% Methanol for 10 min and incubated overnight with the primary antibody (Table 2) in PBS/T 0.1 with 10 % normal swine/ goat or rabbit serum (Dako). Second ...
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bioRxiv - Cell Biology 2021Quote: ... Single cells were separated using a 100 μm strainer and labelled with CD31 antibody (1:200, M082329-2, DAKO) at 4°C for 45 min followed by 30 min staining with a goat anti-mouse secondary antibody conjugated to AlexaFluor 488 or 555 (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 x 104 cells were seeded into each well of a V3 96-well plate (Agilent, Cat. # 101085-004) and cultured 24 h before measuring OCR ...
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bioRxiv - Cell Biology 2020Quote: ... smNPCs were split with accutase and 2×105 cells per well were seeded on Seahorse cell culture microplates (Agilent) one day before the ...
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bioRxiv - Immunology 2022Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Basal OCR and ECAR were measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... deparaffinized sections were subjected to antigen retrieval and processed with the EnVision+ HRP kit (K401111–2, DAKO, Glostrup, Denmark) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 2% BSA (PAA Laboratories GmbH, Germany) and 1:50 dilution of normal goat serum (Dako Denmark A/S, Denmark). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... and (2) the use of an Agilent split/splitless injector with a splitless gooseneck liner with glass wool (Agilent). 350 μL of the reconstituted extract was dried in 400 μL inserts in 1.5 mL chromatography vials after the addition of 40 μL ethoxyamine hydrochloride in pyridine (c = 20 g L-1) ...
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bioRxiv - Molecular Biology 2022Quote: ... The enzyme was heat inactivated at 75°C for 2 minutes and then removed with 1μl of Strataclean resin (Agilent). Capped RNAs were precipitated using 2M ammonium acetate and isopropanol to remove any residual nucleotides ...
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bioRxiv - Biochemistry 2019Quote: ... All final extracts were dried under vacuum (Extractor Plus®, Agilent; settings: 30°C, 2 h, 1000 rpm, VAQ).
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bioRxiv - Biophysics 2019Quote: ... was conjugated with Cy5 maleimide at room temperature for 2 hours and purified with a C18 250x 4.6mm column (Agilent) as previously described (Yin et al. ...
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bioRxiv - Genomics 2019Quote: Both the cDNA and gDNA amplicons were isolated on a 2% TAE gel and bands were confirmed using Agilent’s D1000 ScreenTape and reagents (Agilent) on a 2200 Tapestation system ...
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bioRxiv - Cancer Biology 2020Quote: ... The spectrophotometric data were obtained using a Synergy 2 Multi-Detection Microplate Reader (Agilent Technologies, Santa Clara, CA, USA) at a wavelength of 450 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... following antigen retrieval by boiling for 20 min in target retrieval solution (pH 6.0, Agilent Dako, Cat # 169984-2) and blocking steps ...
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bioRxiv - Cancer Biology 2020Quote: ... following antigen retrieval by boiling for 20 min in target retrieval solution (pH 6.0, Agilent Dako, Cat # 169984-2) and blocking steps ...
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bioRxiv - Immunology 2020Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Plant Biology 2021Quote: ... and 2% Buffer B (0.1% formic acid in Optima grade acetonitrile (Fisher)) using a 1200 series capillary pump (Agilent). Following loading ...
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bioRxiv - Neuroscience 2021Quote: ... Mamu-DR (monoclonal mouse – anti-human HLA-DR/DQ-IgG1, clone CR3/43, cat. no. M077501-2, Agilent Technologies), 1:150 ...
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bioRxiv - Genetics 2020Quote: ... Baseline respiration and maximal respiratory capacity were measured using the Seahorse instrument (XF24 data for Figure 2 and Xe96 data for figure 3) according to manufacturer’s instructions (Agilent). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... Detection of the primary antibodies was carried out using Envision FLEX/HRP secondary antibody (GV80011-2; DAKO, Hamburg, Germany). 3,3′-diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Mpro mutants were generated with QuikChange® II Site-Directed Mutagenesis Kit from Agilent (Catalog #200524), using plasmid pE-SUMO-Mpro as the template ...
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bioRxiv - Developmental Biology 2024Quote: ... the slides were treated with the Dako Real Envision Detection System kit (K500711-2, Agilent, Santa Clara, CA, USA) and counterstained with hematoxylin.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Following permeabilization the cells were washed three times with PBS then blocked with Protein Block (Aligent/Dako, X090930-2). Primary antibodies (ADAR1 (Santa Cruz ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Cell Biology 2024Quote: ... the buffer was exchanged into basic PBS (pH 8.0) with 0.1 % Tween-20 for a 2 h conjugation with 10 µM streptavidin (Prozyme, SA10) and 10 µM fluorescent dye (Lissamine Rhodamine B ethylenediamine ...
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bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... All libraries were sequenced (2×100 bp) using Agilent SureSelect RNA XTHS2 All Exon V8 (Agilent, cat. no. G9991A) according to the standard Agilent protocol.
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bioRxiv - Evolutionary Biology 2023Quote: We sheared DNA to a mean size distribution of ∼20 kb using a Diagenode Megaruptor 2 according to the manufacturer’s protocol, (Denville, New Jersey, USA) and sized sheared DNA on a Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was determined during basal and upon addition of oligomycin (1 µM), carbonylcyanide p-(trifluoromethoxy) phenylhydrazone (FCCP, 2 µM) and rotenone/antimycin (0.5 µM) using the Seahorse XFe24 bioanalyzer (Agilent). Data from each well was corrected to cell number using the Bio Tek Cytation 1 Cell Imaging Multimode Reader and presented as mean +/-SEM.
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bioRxiv - Neuroscience 2023Quote: ... These pUAST-(G4C2)n vectors were amplified with a recombinase-mutated SURE®2 Escherichia coli strain (Agilent Technologies) at 28 °C for 72 hours to prevent repeat length contraction ...
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bioRxiv - Immunology 2023Quote: ... at a density of 2×105 cells/well on a Seahorse XFe96 cell culture plate (Agilent Technologies; 101085-004). Plates were pre-coated with Corning™ Cell-Tak Cell and Tissue Adhesive (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions contained 10 µL of 2× Brilliant III Ultra-Fast SYBR green QPCR master mix (catalog no. 600882; Agilent), 2 µL each of 2 µM PCR primers (see Table S1 for used primers) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were acidified with TFA to a pH < 2 and desalted by C18 using the Bravo AssayMAP platform (Agilent) before enrichment of crosslinked peptides with Fe(III)-NTA cartridges in the Bravo AssayMAP platform as previously described[23].
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bioRxiv - Genetics 2023Quote: ... resulting from Cas12a based trans-cleavage were measured every 2.5 min using a BioTek Synergy 2 microplate reader (Agilent). In a diagnostic specificity assay using field samples ...
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bioRxiv - Physiology 2023Quote: ... followed by Vectastain Elite ABC HRP detection kit (Vector PK-6100) and detection with DAB (Agilent Technologies K346811-2). Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... on a Shimadzu LC2030C Plus 2 machine equipped with a Poroshell 120EC-C18 column (250 x 4.6 mm, Agilent) and a UV/vis detector ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were washed with 0.1% Triton X-100 in PBS and the slides were mounted in fluorescence mounting medium (S302380-2, Agilent). Imaging was performed with Cell Observer Spinning Disk Confocal Microscope using the ZEN software ...
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bioRxiv - Biochemistry 2024Quote: All experiments used 1.5 μL packed bed volume Exp 2 bolt cartridge trapping columns (Optimize Technologies) packed with PLRP (Agilent) solid phase and 150 x 0.3 mm analytical columns packed with 2.6 μm Kinetex core shell particles coated with polar capped C18 (Phenomenex) ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Oxygen consumption rates (OCR ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with this media for 4 hours before plating with the assay media (composed of XF Base Medium Minimal DMEM supplemented with 2 mM glucose and 0.5 mM L-carnitine; Agilent) in the XF96 well plate (Agilent ...
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bioRxiv - Microbiology 2024Quote: ... cultures were transferred to 21 x14.5 x 5.5 cm3 plastic trays on ice and crosslinked three times at 0.75 J/cm-2 using a UV Stratalinker 2400 (Stratagene) to induce covalent bonds between protein and RNA ...