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Citations for Agilent :
1651 - 1700 of 2304 citations for Homeobox Protein Hox B1 HOXB1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Immunodetection of TTR monomers was performed by employing rabbit anti-human TTR polyclonal antibody (Dako) as a primary and goat anti-rabbit antibody labelled with DyLight 680 (SERACARE ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used in the study included: anti-WT1 (Dako, ref M3561, clone 6F-H2), anti-MAFB (BETHYL Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by polyclonal goat anti-mouse/rabbit immunoglobulins horseradish peroxidase (HRP)-conjugated secondary antibodies (Dako) diluted 1:1000 for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... followed by horseradish peroxidase (HRP-) conjugated goat anti-mouse polyclonal antibody (DAKO, Carpinteria, CA, USA) (1:2000 in PBS-Tween 20) ...
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bioRxiv - Molecular Biology 2024Quote: ... a 1:4000 dilution of anti-mouse HRP-conjugated secondary antibody into blocking milk (DAKO) was applied to the membrane for 1h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation with polyclonal goat anti-rabbit IgG/HRP antibody (1:1000; DAKO Cytomation) for 90 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... immunoblots were probed using goat anti-rabbit secondary antibody conjugated to horseradish peroxidase (Agilent P0448), treated with enhanced chemiluminescence detection reagents (Cytiva) ...
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bioRxiv - Cell Biology 2020Quote: ... These DN proteins were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2019Quote: ... Sections were then treated with Proteinase K (20 μg/mL) for 15 minutes for antigen retrieval and blocked with DAKO background reducing protein blocking agent (Agilent, X090930-2) prior to 1 hour incubation of CD206 (1:200 ...
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bioRxiv - Biophysics 2019Quote: ... Protein concentration was determined using UV absorbance at 280 nm on an Agilent 8453 UV/Vis spectrophotometer (Agilent Technologies, Milford, MA, USA).
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bioRxiv - Biophysics 2021Quote: ... dye at self-quenching concentration of 25 mM and used to monitor increase in fluorescence upon addition of ClyA proteins in a fluorescence spectrophotometer (Agilent Cary Eclipse). To make SUVs at first ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with 3% hydrogen peroxide to quench endogenous peroxidase activity and 15 minutes in serum-free protein block (DAKO, Glostrup, Denmark). Sections were then subjected to appropriate antigen retrieval methods (if needed ...
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bioRxiv - Plant Biology 2021Quote: ... at 37⁰C (1:50 protein/trypsin ratio) and cleaned up with a C18 column (OMIX C18, Agilent, Santa Clara, CA, USA). Nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS ...
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bioRxiv - Molecular Biology 2019Quote: The rEDIII protein band was excised from polyacrylamide gel and the sample was prepared for de novo protein sequencing using in-gel digestion according to manufacturer’s protocol (Agilent Technologies, Inc., 2015). Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... The original eGFP-C2-DEF6 plasmid (5) was used to generate the mutant DEF6 proteins by mutagenesis PCR (QuikChange Lightning Multi Site-Directed Mutagenesis Kit, Agilent Technologies, #210516). All mutations were verified by sequence analysis (see Suppl ...
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bioRxiv - Biochemistry 2019Quote: ... IgG was incorporated into sodium dodecyl sulphate polyacrylamide gel and glycans were released from protein using an overnight incubation with PNGase F (ProZyme, Hayward, CA). Released glycans were labelled with 2-aminobenzamide (2-AB ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed three times (3X) in TBST and incubated in blocking solution (Protein Block Serum-Free, Agilent DAKO, CA, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed three times (3X) in TBST and incubated in blocking solution (Protein Block Serum-Free, Agilent DAKO, CA, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasma samples were depleted of high- and mid-abundant proteins using immune-depletion columns (Agilent Multiple Affinity Removal Column MARS3 (Agilent 5188-5218) coupled to the Seppro mouse LC5 SuperMix column (Sigma S5824 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated for 1 h at room temperature with rabbit anti-glial fibrillary acidic protein (GFAP, 1:300; Dako, Carpinteria, CA), rabbit anti-oligodendrocyte specific protein (OSP ...
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bioRxiv - Biophysics 2022Quote: Mass spectrometry was performed with protein samples (∼10 μM) with 10% acetonitrile on a 6100 Series Quadrupole LC mass spectrometer (Agilent Technologies, Inc.), equipped with an electrospray source and operated in the positive ion mode ...
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bioRxiv - Systems Biology 2022Quote: ... the RNA integrity number was 10 as assessed by the Stanford Protein and Nucleic Acid (PAN) Biotechnology Facility using the RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Biophysics 2023Quote: Assessment of the extent of protein oligomerization was based on monitoring absorbance at 550 nm using BioTek Plate Reader (Agilent BioTek NEO2) to reflect turbidity changes induced by protein oligomer formation ...
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bioRxiv - Cancer Biology 2023Quote: ... Blocking with endogenous peroxidase was performed with Protein Block (Novocastra Laboratories) for 5 min and subsequent steps were performed in DAKO autostainer link 48 (Agilent Technologies, Inc.). Slides were counterstained in hematoxylin-eosin ...
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bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Biochemistry 2024Quote: ... the GEF protein was added and the absorbance was recorded for an additional on Biotek Synergy HT or Biotek Synergy H1 (Agilent, Winooski, VT). DMSO was employed as vehicle control ...
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bioRxiv - Immunology 2021Quote: ... and placed on a rocker platform for two 5 minute washes followed by a TBS rinse before being returned to humidifying chamber to be incubated with serum free protein block (Dako, Santa Clara, CA) for 20 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... Translating mRNA bound to the GFP-tagged ribosomal subunit protein L10a was then pulled-down and purified (Absolutely RNA Microprep Kit, Agilent Technologies, Wilmington, DE). RNA-Seq libraries were prepared from 1 μg of purified RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina with NEBNext Poly(A ...
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bioRxiv - Biochemistry 2021Quote: ... Protein intact mass was determined using a 6530 electrospray ionisation quadrupole time-of-flight mass spectrometer (Agilent Technologies Inc. – Palo Alto, CA, USA). The instrument was configured with the standard ESI source and operated in positive ion mode ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were incubated at room temperature for 20 minutes and protein content was measured using a spectrophotometer (Model: Cary 60 UV-Vis, Agilent Technologies, CA, USA) at 595 nm ...
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bioRxiv - Physiology 2024Quote: ... To analyze the deuterium enrichment of alanine in the protein fractions we used Gas Chromatography-Mass Spectroscopy (7890A GC-Agilent 5975C MS, Agilent, Santa Clara, CA)52,53.
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bioRxiv - Cancer Biology 2023Quote: ... sections were washed with tris buffered saline with tween (TBST) for 10 min before unspecific protein binding was blocked using 10 % goat serum (Dako, X0907, CA, USA) in 3 % bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were heated with sodium citrate (pH=6) for antigen retrieval and blocked with a Protein Block reagent (Dako, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... The non-specific binding of antibodies was blocked using 10% Normal Goat Serum (DAKO, Glostrup, Denmark) in PBS for 20 minutes at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, Dako, Japan) and HRP goat anti-rat IgG antibody (Biolegend ...
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bioRxiv - Microbiology 2019Quote: ... bound Igs were detected with Fluorescein isothiocyanate (FITC)-conjugated rabbit anti-mouse Ig antibody (Dako Cytomation). To quantify total IgG levels ...
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bioRxiv - Microbiology 2019Quote: ... This was followed by incubation with the primary antibody (diluted 1:1,000 in dilution buffer; Dako) for 60 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Dako, Agilent Technologies, Santa Clara, California) for 1 h at room temperature diluted in TBS-T containing 5% non-fat milk ...
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bioRxiv - Molecular Biology 2020Quote: ... US Biological Life Sciences) and secondary antibody Rabbit Anti-Goat Ig/HRP (1:5,000, #P0160, Dako). Immunoblotting for α-Tubulin was performed using polyclonal Rabbit Anti-α-Tubulin antiserum as primary antibody (1:4,000 ...
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bioRxiv - Genomics 2020Quote: ... LECs were washed twice in DPBS and stained with mouse anti-human Ki-67 antibody (Dako) diluted 1:800 in FACS buffer (DPBS with 1mM EDTA and 2% FBS ...
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bioRxiv - Systems Biology 2019Quote: ... plasma samples were assayed using quantitative western blotting using a polyclonal antibody to human RBP4 (Dako) and human TTR (Dako ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies and dilutions used are as follows: guinea pig anti-insulin (DAKO, A0564, 1:1000), mouse anti-glucagon (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... The following antibodies were incubated overnight at 4°C: rat anti-human CD31 (DAKO, M082329-2), rabbit anti-human S1PR1 (Santa Cruz ...
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bioRxiv - Physiology 2020Quote: ... Transverse formalin-fixed lung sections were stained with an anti-smooth muscle actin antibody (DAKO M0851) or Verhoeff’s van Gieson stain (EVG ...
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bioRxiv - Neuroscience 2019Quote: ... GFAP immunohistochemistry for astrocytes (rabbit anti–mouse GFAP polyclonal antibody 1:13000 for 24 min; DAKO) and IBA-1 for microglia (rabbit polyclonal antibody 1:1000 for 24 min ...
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bioRxiv - Genetics 2019Quote: ... washed twice with PBS for 5 minutes and then incubated with anti-rabbit biotinylated antibody (Dako) at a concentration of 1:500 in PBS for 30 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: LC-MS spectra of antibodies and ADCs were measured on the same MS spectrometer (Agilent 6230) with a THERMO MAbPac RP column (4 μm ...
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bioRxiv - Cancer Biology 2019Quote: ... Tissue sections were incubated with primary antibodies to anti-human Ki-67 (Clone MIB-1, DAKO) and cleaved caspase-3 (Cell Signaling ...
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bioRxiv - Physiology 2020Quote: ... BrdU positive cells were detected with an anti-BrdU primary antibody diluted 1:100 (DAKO Cytomatation) and with an anti-mouse-Alexa488 conjugated diluted 1:100 (Thermofisher) ...
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bioRxiv - Genomics 2020Quote: ... with the use of the commercially available antibody clones 22C3 (Dako/Agilent, Santa Clara, CA, USA) or SP142 (Ventana ...