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Citations for Agilent :
1551 - 1600 of 2304 citations for Homeobox Protein Hox B1 HOXB1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... α-smooth muscle actin (SMA, fibroblast/myofibroblast marker, mouse monoclonal antibody, clone 1A4; Dako/Agilent), CD163 (monocyte/macrophage marker ...
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bioRxiv - Pathology 2024Quote: ... α-smooth muscle actin (SMA, fibroblast/myofibroblast marker, mouse monoclonal antibody, clone 1A4; Dako/Agilent), CD163 (monocyte/macrophage marker ...
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bioRxiv - Neuroscience 2024Quote: ... Western blot was performed with anti-tau rabbit polyclonal antibody (1:1000, Dako A0024) and anti-rabbit HRP-conjugated secondary antibody (1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated in primary antibody β-catenin ((M3539, Dako, CA, USA, 1:600), IKKα (GWB-662250 ...
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bioRxiv - Bioengineering 2021Quote: ... 10-20 µl injection volume depending on protein concentration) were injected on a Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) at a flow rate of 100 µl/min using solvent A (0.1% formic acid and 0.05% trifluoroacetic acid in water ...
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bioRxiv - Molecular Biology 2020Quote: ... All samples were depleted of the 7 most abundant proteins with the multiple affinity removal system (MARS Hu7 spin cartridge) according to the manufacturers protocol (Agilent Technologies). Samples were further reduced using 4 mM DTT ...
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bioRxiv - Microbiology 2019Quote: ... MBP-fusion proteins were purified with amylose resin following the manufacturer’s instructions (GE Healthcare, Pierce, Agilent Technologies and New England Biolabs). MBP-fusions (1 µg each in 100 µl of 1% NP40 ...
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bioRxiv - Cell Biology 2020Quote: Zyxin and Pxl1 LCR proteins were expressed in Escherichia coli strain BL21-Codon Plus (DE3)-RP (Agilent Technologies, Santa Clara, CA) with 0.5 mM isopropyl β-D-1-thiogalactopyranoside for 16 h at 16 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were prepared according to the instruction of the Agilent protein 80 kit and analyzed on a 2100 Bio-analyzer device (Agilent technologies). The ATPase activity of FSS-PscN was determined by measuring the release of total phosphate at 37°C for 30 minutes which was detected by the malachite green method (Casabona et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... approximately 8 µg of protein was injected on a Zorbax 300SB-C18 column (5 µm, 300Å, 1×250mm IDxL; Agilent Technologies) and separated using a 30 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Immunology 2020Quote: ... followed by a quick rinse in TBS before being returned to the black chamber to be incubated with serum free protein block (Dako X0909) for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of 2 – 4 mg/ml protein samples were applied to a column using the 1260 Infinity HPLC system (Agilent Technologies) coupled to a MiniDawn Treos detector (Wyatt Technologies ...
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bioRxiv - Immunology 2022Quote: ... brucei Heat Shock Protein 70 (TbHSP70) (Kind gift from Professor Keith Matthews, Edinburgh, UK) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s haematoxylin.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected using tryptophan fluorescence (λex=280 nm; λem=315 nm) using a fluorescence detector (Agilent technologies 1200 series, G1321A). The peak heights of the monomeric hsFPN peaks were used to assess the stability by normalizing heights to the corresponding value from samples incubated at 4 °C (100% stability) ...
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bioRxiv - Cancer Biology 2020Quote: ... the serum samples were immunoaffinity depleted of the 14 most abundant proteins using IgY14 LC20 (cat No. 5188-6557, Agilent, USA). The depletion was performed on an Agilent 1260 HPLC (1260 Infinity ...
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Biochemistry 2021Quote: Serum samples were depleted of the top 14 interfering high-abundant proteins using the Multiple Affinity Removal Column Human 14 (Agilent, USA). Then ...
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bioRxiv - Immunology 2023Quote: ... brucei parasites using a polyclonal rabbit antibody raised against T. brucei luminal binding protein 1 (BiP) (J. Bangs, SUNY, USA) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s Haematoxylin ...
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bioRxiv - Molecular Biology 2023Quote: The above 12 OR genes linked to a wild-type internal ribosome entry site from the encephalomyocarditis virus (EMCV IRES) and the mCherry fluorescent protein gene (Shaner et al., 2004) were inserted into a modified pBlueScript KS (Agilent/Stratagene) called plasmid “L” (Zboray et al. ...
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bioRxiv - Microbiology 2023Quote: ... approximately 2 µg of protein was injected on a Zorbax Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) and separated using a 15 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescence spectroscopy measurements of 2 μM of each protein sample were obtained using the Varian Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies) with an excitation wavelength of 280 nm and recording the emission spectra between 300 nm and 400 nm.
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bioRxiv - Cancer Biology 2023Quote: ... together with 20 μl of pre-washed Protein A/G Dynabeads Chromatin digestion was assessed using TapeStation electrophoresis system (Agilent Technologies). Desired DNA digestion yielded a mono- and poly-nucleosome pattern with a range of DNA fragments 100-1000 bp ...
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bioRxiv - Cell Biology 2023Quote: ... and GST-SDPN-1 SH3 (P479L) fusion proteins were expressed in the ArcticExpress strain of Escherichia coli (Stratagene, La Jolla, CA). Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce ...
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bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
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bioRxiv - Genomics 2023Quote: Each purified GST-tagged DBD stock was validated for DNA-binding specificity using 4×44k universal protein-binding microarrays (PBMs) (Agilent Technologies), as described previously42 ...
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bioRxiv - Biophysics 2023Quote: ... The monodisperse sample solutions of proteins were injected onto a size exclusion column (David and Perez 2009) (SEC-3, 150 Å; Agilent) using an Agilent HPLC system and eluted into the capillary cell at a flow rate of 0.3 ml min-1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slides were then treated with 3% hydrogen peroxide for 10 min and then blocked with Serum-Free Protein Block (Dako, X0909) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed thrice with 0.1% (v/v) tween-20 / PBS and incubated in Serum Free Protein Block (DAKO, cat.#X0909) for 1h at 21°C ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase was blocked using Peroxidase block (Refine Kit) followed by Protein block (X090930-2, Agilent Technologies Inc., Santa Clara, CA). Primary antibodies were applied at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Free-floating brain sections (8-14 µm thick) were mounted onto slides and blocked with Protein Block (Serum-Free, Dako, CA) for 1h at RT ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Microbiology 2024Quote: ... approximately 2 μg of protein was injected on a Zorbax Poroshell 300SB-C8 column (5 μm, 300Å, 1x75mm IDxL; Agilent Technologies) and separated using a 15 min gradient from 5% to 80% solvent B at a flow rate of 100 μl/min (solvent A ...
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bioRxiv - Cell Biology 2019Quote: ... for 1hr and incubated OVN at 4°C with indicated primary antibodies: αSMA (Dako, M0851); Desmin (Abcam ...
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bioRxiv - Immunology 2021Quote: ... followed by 5 μg/ml anti-MPO antibodies (A0398 polyclonal, Agilent Technologies, Santa Clara, CA) or 40 μg/ml isolated serum IgG ...
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bioRxiv - Cell Biology 2022Quote: ... Goat anti-mouse and anti-rabbit horseradish peroxidase (HRP)-conjugated antibodies were from Dako (Agilent).
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bioRxiv - Biochemistry 2022Quote: ... and secondary antibodies (HRP goat anti-Mousse-Dako #P0447, HRP goat anti-Rabbit-Dako #0448). Antibodies were diluted in TBS (0.1% Tween20-1% fat dry milk ...
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bioRxiv - Neuroscience 2021Quote: ... UK) overnight and anti-mouse IgG alkaline phosphatase-linked secondary antibody (1:5,000; Dako, USA). Membranes were incubated with CDP-Star chemiluminescent substrate (Thermo Fisher ...
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bioRxiv - Immunology 2019Quote: ... staining was developed with the EnVision System-HRP for mouse primary antibodies (Dako, Carpinteria, CA). Sections were counterstained with hematoxylin ...
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bioRxiv - Immunology 2019Quote: ... the primary antibodies were omitted or replaced by IgG isotype control (Cat ID: 559073, Dako). The fluorescent images were imaged using a Leica TCS SP5 II confocal microscope (Leica ...
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bioRxiv - Genomics 2019Quote: ... and the Flex mouse anti-human CD31 antibody (clone JC70A, Dako North America, Carpinteria, CA) for 90 min ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies coupled with horseradish peroxidase (HRP): goat anti-mouse (Dako P0447, WB: 1/1000), goat anti-rabbit (Dako P0448 ...
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bioRxiv - Cancer Biology 2019Quote: ... Goat anti-mouse polyclonal antibodies conjugated with horseradish peroxidase (cat. #P0447, Agilent, Santa Clara, USA) were used as secondary antibodies.
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bioRxiv - Biochemistry 2019Quote: ... Plasma (0.7 mL) was incubated with polyclonal anti-fibrinogen or anti-VWF antibody-coated (Dako) Dynabeads (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... sections were incubated in secondary antibody for 30 min (EnVision+ System-HRP Labelled Polymer; Dako) and the staining was visualized with DAB (EnVision+ System) ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5 ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated with secondary antibodies HRP Labelled Polymer Anti-Rabbit (Dako EnVision+ System; Cat#: K4002) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... and respective secondary antibody before being detected using Liquid DAB (Agilent, Santa Clara, CA, USA) or UltraMap DAB anti-Rb Detection Kit (Ventana Medical Systems ...