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Citations for Agilent :
1451 - 1500 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The quality control of cDNA and obtained final libraries was performed using a Bioanalyzer High Sensitivity DNA Analysis assay (Agilent). Library quantification was performed using the Collibri™ Library Quantification Kit (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... viral protein in the virus-infected cells was detected by ELISA assay using anti-SARS-CoV-2 nucleocapsid mAb (40143-R001, SinoBiological) and HRP-conjugated goat anti-rabbit pAb (P0448, Dako). After 10 min incubation with TMB substrate ...
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bioRxiv - Cancer Biology 2022Quote: ... and its quality was evaluated with the RNA 6000 Nano Assay on a Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). Libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit protocol (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... The size-distribution of cDNA libraries was measured using the Agilent high sensitivity D5000 assay on a Tapestation 4200 system (Agilent). cDNA libraries were quantified using a Qubit high sensitivity dsDNA assay ...
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bioRxiv - Cancer Biology 2020Quote: ... the cell media was replaced with the assay media and ran using the Seahorse XF Cell Mito Stress Test and ATP Rate Assay on a Seahorse XFe96/XF96 analyzer (Agilent). Hoechst 33342 staining (ThermoFisher ...
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bioRxiv - Synthetic Biology 2021Quote: ... Concentration and cDNA size was checked for select representative wells using a High Sensitivity DNA5000 assay for the Tapestation 4200 (Agilent). cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell was tagmented by adding 1 µl TD and 0.5 µl ATM from a Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Fragment size and quality was assessed by the Agilent TapeStation 4200 using the High-Sensitivity DNA ScreenTape Assay (Agilent, USA) on TruSeq input DNA pre- and post-sonication ...
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bioRxiv - Genetics 2020Quote: ... ribosome profiling library size distributions were checked on the Bioanalyzer 2100 using a high sensitivity DNA assay (Agilent; 5067-4626), multiplexed and sequenced on an Illumina HiSeq 2500 producing single end 1×51nt reads ...
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bioRxiv - Plant Biology 2021Quote: ... DNA contamination and first quantification were performed via RNA Pico Assay for the 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA). Quantification was verified via a Qubit RNA BR (Broad-Range ...
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bioRxiv - Microbiology 2021Quote: ... Mass validation of peptide products from in vitro cleavage assays or in vivo endogenous production was performed by LC-MS/MS on a 6546 LC/Q-TOF (Agilent) employing a 1290 Infinity II LC system using an Atlantis dC18 column (5 μm ...
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bioRxiv - Microbiology 2021Quote: ... A second clean up with magnetic beads was performed and the libraries were quantified using the ThermoFishrer High Sensitivity dsDNA Qubit assay and validated using the TapeStation (Agilent) with the DNA100 screentape assay ...
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bioRxiv - Cell Biology 2020Quote: ... followed by washes in buffered Seahorse Assay medium (pH 7.4) and subsequent metabolic testing according to the manufacturer’s protocol (Agilent Seahorse XF96). Oligomycin (2 μM ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Library concentration was determined using Qubit and average fragment size was estimated using DNA HS assay on bioanalyzer 2100 (Agilent) before pooling libraries at equimolar ratio ...
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bioRxiv - Neuroscience 2021Quote: ... Size and concentration of the RNA-seq libraries was measured by the Agilent 2100 Bioanalyzer using the DNA 1000 assay (Agilent) before loading onto the sequencer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we quantified the libraries using Qubit DNA Assay and assessed the fragments using DNA TapeStation D1000 Screen Tape (#5067-5582,5583, Agilent Technologies). We finally clonally amplified the quantified libraries on a cBOT and sequenced them on the HiSeq X with 150bp paired end chemistry.
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bioRxiv - Developmental Biology 2020Quote: ... Quality of submitted RNA samples and the resulting cDNA libraries was determined by ScreenTape Assay on a 4200 TapeStation (Agilent). Prepared libraries were submitted for single-ended 75 bp sequencing on an Illumina HiSeq 4000 (Illumina).
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bioRxiv - Cancer Biology 2021Quote: Metabolic flux assays measuring oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were performed using the Seahorse XFe96 Analyzer (Agilent). Patient-derived GBM cells were seeded at a density of 1 to 1.5 × 104 cells per well in NSC medium in laminin-coated Seahorse XFe96 cell culture microplates ...
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bioRxiv - Microbiology 2020Quote: ... The integrity of the RNA was checked both in a 1.2% agarose gel and with RNA6000 Nano Assay using the Agilent 2100 Bioanalyzer (Agilent Technologies).
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bioRxiv - Microbiology 2021Quote: ... and concentration of each IVT standard were confirmed using the Agilent Bioanalyzer RNA 6000 Nano assay (Agilent, Santa Clara, CA) prior to dilution in nuclease-free water to working concentrations.
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bioRxiv - Genomics 2021Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and fragment size was checked using the High Sensitivity D1000 ScreenTape assay on a Tapestation 4200 (Agilent) to ensure that fragment sizes were distributed normally about 500 bp ...
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bioRxiv - Genomics 2021Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and fragment size was inspected using the High Sensitivity D1000 ScreenTape assay on a Tapestation 4200 (Agilent). Libraries were sequenced using a NextSeq 500 or NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... The cfDNA concentration was determined using an Agilent 4200 TapeStation System with the Cell-free DNA ScreenTape Analysis assay (Agilent).
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bioRxiv - Molecular Biology 2022Quote: ... whole protein lysates for each well were quantified by BCA assay and total protein amounts were used for normalization of Seahorse data using Wave Software (Agilent). Basal OCR ...
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bioRxiv - Cell Biology 2022Quote: Optimal seeding density and FCCP concentration without toxicity response were tested before starting the Seahorse XF Cell Mito Stress Test assay (Agilent). A cell density of 4×103 cells per well and a FCCP concentration of 2 µM was determined and used for the following assays ...
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bioRxiv - Genomics 2022Quote: ... The cfDNA concentration was determined using an Agilent 4200 TapeStation System with the Cell-free DNA ScreenTape Analysis assay (Agilent).
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bioRxiv - Neuroscience 2022Quote: ... RNA integrity was assayed by electropherogram in a Bioanalyzer using a total RNA Assay with a Pico/Nano Chip (Agilent).
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bioRxiv - Plant Biology 2023Quote: ... Library yield was quantified using the Qubit dsDNA HS assay and insert size was determined using the 2100 Bioanalyzer High Sensitivity DNA chip (5067-4627, Agilent Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... then RNA quality and concentration were determined with the RNA600 Pico Assay using the Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and ND-200 nanodrop (NanoDrop Technologies Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... Library quality and concentration was determined with the High Sensitivity DNA assay using the Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and Qubit® dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: Mitochondrial and glycolytic function was analyzed with the Seahorse Cell Mito Stress Test and Seahorse Real-Time ATP Rate Assay on a Seahorse XFe24 Extracellular Flux Analyzer (Agilent). Neurons were treated on day 11 of culture with 500 μL of fresh neuronal media plus 500 μL of astrocytic conditioned media of varying treatment ...
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bioRxiv - Physiology 2022Quote: ... The concentration of RNA was quantified with an N50 Nanophotometer (Implen Inc.) and RNA integrity evaluated with a TapeStation RNA assay (Agilent). Following DNase treatment ...
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bioRxiv - Molecular Biology 2022Quote: ... and the integrity and size distribution of the RNA was assessed using mRNA Nano series 2 assay (G2938, Agilent Technologies).
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bioRxiv - Neuroscience 2022Quote: ... Retinal disks were incubated for 30 minutes at 37°C prior to running the assay using a Seahorse XF96 Analyzer (Agilent). During the assay ...
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bioRxiv - Neuroscience 2022Quote: ... RNA integrity was validated using the Agilent RNA 6000 Pico Assay according to the manufacturer’s instructions (Agilent Technologies, Waldbronn, Germany). First-strand cDNAs from the total RNA purified from each cell population were synthesized using the RevertAid H Minus First-Strand cDNA Synthesis Kit (Fermentas by Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The fragment was further analysed with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies, Inc, CA, USA). PCR conditions for enrichment and barcoding reactions were as follows ...
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bioRxiv - Physiology 2024Quote: ... MLO-Y4 cells with Pparγ silenced with siRNA (γY4KD) and their scrambled control (Scrl) were plated 6 hrs before the assay onto Seahorse cell culture microplates (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were then assessed for appropriate amplicon size and complete elimination of free primer peaks using the High Sensitivity ScreenTape Assay on the Tape Station2200 (Agilent) and quantified using the qPCR-based quantification in order to ensure only NGS-compatible amplicon was quantified using the Library Quant ROX Low Kit (Kapa Biosystems ...
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bioRxiv - Immunology 2023Quote: ... The quality of the RNA was assessed using a High Sensitivity RNA ScreenTape Assay with a 4200 TapeStation System (Agilent). mRNA-seq library preparation was performed with SMART-Seq® v4 PLUS Kit (R4000752 ...
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bioRxiv - Microbiology 2023Quote: ... Quality control of the RNA preparation was checked using an Agilent 2100 Bioanalyzer System and Qubit RNA IQ Assay (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... was performed for all the samples using the Agilent Bioanalyzer High Sensitivity DNA assay in the 2100 expert software (Agilent). All the samples passed the initial QC with a cDNA size 700-1,500 bp.
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bioRxiv - Developmental Biology 2024Quote: ... Quantity and size distribution of cleaned cDNA was checked using the Bioanalyzer High Sensitivity Assay (Agilent Genomics, Cat: 5067-4626). Libraries for sequencing were prepared from 600pg cDNA input using Nextera XT library preparation kit (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... The quality and yield of fully-barcoded DNA primers was evaluated with total RNA Pico Assay (Agilent Technologies, 5067-1513) and by fluorescent in situ hybridization (FISH ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: All hydrolysis assays were performed in 96-well plates at 30 °C in a BioTek Synergy H1 microplate reader (Agilent). Production of GDP ...
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bioRxiv - Cell Biology 2024Quote: ... and extracellular acidification rates (ECAR) were measured in XF DMEM assay medium with pH adjusted to 7.4 (103680-100, Agilent Technologies) containing 10 mM glucose (103577-100 ...
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bioRxiv - Plant Biology 2024Quote: ... Number of biological replicates per library ranged between RNA quality was assessed using Tape station High Sensitivity RNA assay (Agilent). 0.5 - 1 ug of total RNA proceeded to library preparation ...
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bioRxiv - Cell Biology 2024Quote: ... Concentration and insert size distribution for both the gene expression library and the cell multiplexing library were determined with a BioAnalyzer High Sensitivity DNA Assay (Agilent). Sequencing was performed on a NovaSeq 6000 on multiple flowcells with a paired-end 150 bp configuration ...
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bioRxiv - Cell Biology 2023Quote: ... medium was removed and cells were washed according to manufacturer’s protocol with Seahorse assay medium consisting of DMEM (103575-100, Agilent, USA) with 10 mM glucose ...
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bioRxiv - Bioengineering 2024Quote: ... The size of purified DNA was profiled with the genomic DNA ScreenTape assay (range: 200∼60000 bp) via the 4200 TapeStation system (Agilent).
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bioRxiv - Synthetic Biology 2022Quote: ... 10 µl of protein sample in assay buffer (20 mM sodium phosphate, 20 mM sodium chloride, pH 8) was injected onto a C4 column (Agilent) and eluted in a water (1% FA ...
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bioRxiv - Genomics 2023Quote: ... Illumina-compatible paired-end sequencing libraries were prepared following 10X Genomics recommendations and validated on an Agilent 2100 BioAnalyzer with the DNA 7500 assay (Agilent). The library was sequenced on NovaSeq 6000 (Illumina ...