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Citations for Agilent :
101 - 150 of 3722 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: WT and CCN4-KO melanoma cells were cultured overnight (1 × 104 cells/well) in Seahorse XFe96 cell culture microplates (Agilent, CA) with complete DMEM medium ...
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bioRxiv - Microbiology 2022Quote: ... and GFAP (rabbit anti-human GFAP 2033X; 1:2000 dilution; DAKO) specific Ab (conjugated to horseradish peroxidase ...
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bioRxiv - Developmental Biology 2023Quote: ... Antibodies used: Anti-Human CD31 Clone JC70A (Dako, M0823, 1:500); Monoclonal Anti-Actin ...
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bioRxiv - Immunology 2023Quote: ... and human α1 antitrypsin (diluted 1:1000; Dako, Carpinteria, CA, USA). Next ...
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bioRxiv - Cancer Biology 2024Quote: ... a monoclonal mouse anti-human Ki-67 antibody (1:25, Dako), a rabbit monoclonal anti-CHGA antibody (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCRs were performed on WT genomic DNA with PfuUltra II (Agilent). The 50 μl PCR mix contained 6-10 μl dNTP mix (2.5 mM each) ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex formed was then purified by size exclusion chromatography using a SEC5 column (Agilent). The fraction corresponding to the top peak was recovered ...
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bioRxiv - Cancer Biology 2021Quote: HLA-restriction of antigen recognition was tested in anti-IFN-γ ELISpot using autologous DCs loaded with the relevant peptide or without peptide (negative control) and blocking antibodies against HLA class I and II (both Dako). DCs were incubated with peptides overnight and the next day anti-HLA mABs were added to ELISpot plates for 1 hour prior to the addition of expanded cells at a ratio of 1 DC to 50 expanded cells ...
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bioRxiv - Immunology 2023Quote: ... in the α3 domain of HLA-A*02:01 described to abrogate binding of CD8 (13) were changed by site-directed mutagenesis (Agilent).
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and a 300×7.8 mm Hi-Plex Exchange column (Agilent, USA) was used to quantify glucose ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-human CD20 (Dako, clone L26, 1:1000, pH 6 retrieval), mouse anti-human FOXP3 (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were incubated with rabbit anti-human PGP 9.5 (DAKO 1:100) overnight at 4°C.
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bioRxiv - Immunology 2022Quote: ... GFAP was detected with polyclonal rabbit-anti human GFAP (1:500, Agilent) and donkey anti-rabbit IgG AF55 detection (1:500 ...
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bioRxiv - Immunology 2020Quote: ... or rabbit anti-human IgA/HRP (Dako: cat. P0216, 1:5’000 dilution). Assays were developed by addition of 3,3’,5,5’-Tetramethylbenzidine (TMB ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with primary antibodies against human CD31 (1:50, JC70A, Dako), PDGFRβ (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody was mouse anti-human Aβ (Dako #M0872; 1:400) and the secondary antibody was Vectastain ABC kit anti-mouse secondary antibody (Vector Laboratories #PK-4002 ...
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bioRxiv - Genetics 2024Quote: ... cells were incubated with the anti-human vWF (1:100, A0082, Dako) or VE-cadherin (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Glial Fibrillary Acidic Protein (GFAP) (1:1000, Z0334, Dako), Htt (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Glial Fibrillary Acidic Protein (GFAP) (1:1000, Z0334, Dako), Huntingtin anti-N17 (1-8 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial acid fibrillary protein (GFAP) (Dako, 1: 1000). A classical astrocyte marker (Gomes-Leal et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-glial fibrillary acidic protein (1:500, Z0334, DAKO) and anti-F4/80 (1 μg mL-1 ...
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bioRxiv - Neuroscience 2024Quote: ... 1:200 rabbit Glial Fibrillary Acidic Protein (Dako, Z0334), 1:200 rabbit Iba1 (Wako ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cytokeratin 5/6 (mouse monoclonal) at 1:100 to 1:200 dilution for zebrafish tissue and 1:100 dilution for human tissue (Dako), GATA 3 (SAB2100898 ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoreactions were visualized with a monoclonal APAAP-complex and a fuchsin-based substrate-chromogen system (Dako). Counterstaining was performed with Mayer’s hemalum (Merck) ...
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bioRxiv - Biochemistry 2024Quote: ... ab109401 (1:5000)) or and anti-total Tau antibody (rabbit anti-human Tau, Dako, #A0024 (1:5,000)) diluted in 3% BSA in PBS-T overnight at 4°C shaking ...
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bioRxiv - Molecular Biology 2021Quote: WT or mutant nsp1 was expressed using BL21-CodonPlus (DE3)-RIPL cells (Agilent) using Overnight ExpressTM instant TB medium (Novagen) ...
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bioRxiv - Neuroscience 2020Quote: The MAPK/ERK pathway was assayed in HEK293 cells with the Elk1 trans-reporting system (PathDetect, Agilent). HEK293 cells were maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: AAV1/2 viruses were produced by large-scale triple CaPO4 transfection of HEK293-AAV cells (#240073, Stratagene) as described previously (Van Loo et al. ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... AAV9-CAG-tdTomato and AAV-KP1-CAG-tdTomato were produced in HEK293 cells (RRID: CVCL-6871, Agilent) by an adenovirus-free plasmid transfection method and purified by two rounds of cesium chloride density-gradient ultracentrifugation followed by dialysis as described previously.53 AAV9 and AAV-KP1 helper plasmids were provided by J ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-glial fibrillary acidic protein (GFAP; Z0334, Dako, 1:20000/1:100000), rabbit anti-glutamine synthetase (GS ...
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bioRxiv - Neuroscience 2022Quote: ... Ki67 (1:1000) and glial fibrillary acidic protein (GFAP) (1:1500) from Dako Cytomation (Glostrup ...
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bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: The murine and human proteins (variants R166H and R168H, respectively) were produced in E.coli BL21 CodonPlus(DE3) RIL (Agilent Technologies) carrying the plasmid pHisTrx-mα-DGN and pHisTrx-hα-DGN ...
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bioRxiv - Immunology 2023Quote: ... IgG from 100 µl of human plasma (∼100 µg/100 µl) was pre-enriched using the Protein G AssayMAP Bravo (Agilent) technology as described above ...
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bioRxiv - Neuroscience 2024Quote: ... Concentrated protein samples were then immunodepleted using a multi-affinity removal spin cartridge human-14 (0.45 mL, Agilent Technologies, UK) to remove high abundance proteins according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-human CD68 (Dako, clone PG-M1, 1:750, pH 6 retrieval), mouse anti-human CD20 (Dako ...
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bioRxiv - Cancer Biology 2022Quote: ... these slides were incubated with rabbit anti-human PGP 9.5 (DAKO 1:100) overnight at 4°C to mark the nerve fibers ...
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bioRxiv - Pathology 2020Quote: ... A polyclonal rabbit anti-human CD3 antibody (1:200; Agilent Technologies Inc, CA) was applied for 15 min and used with Leica Polymer Refine Detection kit to complete the staining.
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bioRxiv - Physiology 2021Quote: ... Anti-human CD68 (mouse monoclonal IgG3, clone PG-M1, Dako, dilution 1/100) and anti-human IL-1β (rabbit polyclonal antibody ...
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bioRxiv - Physiology 2024Quote: ... CD68 (mouse anti human, 1:100, cat. no.: M0718, Dako Norden, Glostrup Denmark), PDGFR-α (goat anti human ...
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bioRxiv - Immunology 2022Quote: ... Phe322 was substituted for the Tyr322 residue of WT and G307S DNAM-1 by using QuikChange II Site-Directed Mutagenesis Kits (Agilent technologies, Palo Alto, CA) with primers (5′-GAGAGGATATTTTTGTCAACTATCCAACC-3′ and 5′-GGTTGGATAGTTGACAAAAATATCCTCTC-3′) ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies against human CD3 (1:100; #A045229-2) and CD68 (1:400; #M0876) were obtained from Dako, UK ...
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bioRxiv - Bioengineering 2021Quote: ... and Iba-1 (ionized calcium binding adaptor protein, 1:5000, Dako, Cat#019-19741). Mouse anti-SARS-CoV-2 N monoclonal antibody (1:5000 ...
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bioRxiv - Pathology 2020Quote: ... The self-complementary scAAV9-CB-SMN vector was produced by calcium phosphate transfection of HEK293-AAV cells (Agilent) with pAAV-CB-SMN[57] and pDF9 plasmids ...
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bioRxiv - Biochemistry 2021Quote: Serum samples were depleted of the top 14 interfering high-abundant proteins using the Multiple Affinity Removal Column Human 14 (Agilent, USA). Then ...
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bioRxiv - Synthetic Biology 2020Quote: Mutagenesis was performed on the pET21b(+)-TrpR-Wt plasmid using the QuikChange technique (Stratagene) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-glial fibrillary acidic protein (GFAP, 1:1,000, DAKO), mouse anti-4A4 (1:2,000 ...
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bioRxiv - Neuroscience 2021Quote: ... glial fibrillary acidic protein (GFAP) diluted 1:2000 (Dako, Z0334), PDGFRA diluted 1:200 (Cell Signaling ...