Labshake search
Citations for Agilent :
1251 - 1300 of 6679 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... A two-stage PCR protocol for site-directed mutagenesis was adapted from Stratagene [25] ...
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bioRxiv - Genetics 2023Quote: ... The PCR products were analyzed with the Agilent D5000 Screen Tape System (Agilent Technologies ...
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bioRxiv - Biophysics 2023Quote: ... All variants were constructed according to the standard PCR-based mutagenesis strategy (Stratagene) and verified by DNA sequencing.
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bioRxiv - Evolutionary Biology 2023Quote: ... 10µl of Brilliant III Ultra-Fast SYBR® Green PCR Master Mix (Agilent), and finally 25 ng of DNA sample from infected oysters or Milli-Q water (non-template control ...
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bioRxiv - Cancer Biology 2023Quote: ... The oligo pool was PCR amplified using Herculase II Fusion DNA Polymerase (Agilent) and cloned into lentiCRISPRv2 puro vector (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were digested by DpnI enzyme for 2h at 37°C (Agilent) and loaded on a 1% Agarose Gel ...
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Genomic dissection and mutation-specific target discovery for breast cancer PIK3CA hotspot mutationsbioRxiv - Genomics 2024Quote: ... PCR products were measured and visualized using a D5000 ScreenTape System (Agilent, 5067) (Figure S4).
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bioRxiv - Evolutionary Biology 2024Quote: ... Indexing PCRs were performed with Taq Pfu Turbo Cx HotStart DNA polymerase (Agilent) with the following cycling conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... using a skirted PCR plate to facilitate automation (Agilent Bravo liquid handling system). Library pools then underwent qPCR quantification and libraries were adjusted to a concentration of 2nM ...
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bioRxiv - Cancer Biology 2024Quote: ... All PCR reactions were performed with Herculase II Fusion DNA Polymerase (Agilent K3467). The first PCR (PCR1 ...
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bioRxiv - Genomics 2021Quote: ... high-sensitivity DNA kits and RNA pico kits (Agilent, Tokyo, Japan) were used ...
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bioRxiv - Plant Biology 2020Quote: ... using a 1200 series capillary and eluted directly into a 6550 series quadrupole time-of-flight mass spectrometer (Agilent Technologies) with a 1200 series nano pump as described [9] ...
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bioRxiv - Cell Biology 2020Quote: ... Frozen samples were spread to a thin layer on petri dishes pre-cooled on dry ice and UV-crosslinked 3 times at 400 mJ/cm2 using a Stratalinker 2400 (Stratagene). All samples were stored at −80°C until tissue lysis.
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bioRxiv - Microbiology 2021Quote: ... Fatty acid methyl esters were identified by their mass spectrum and retention time and quantified by Mass Hunter Quantification Software (Agilent) and the calibration curve generated with fatty acid methyl esters standards mix (Sigma CRM47885) ...
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bioRxiv - Microbiology 2019Quote: ... sucrose and maltose was determined through gas chromatography coupled to electron impact ionization/time-of-flight mass spectrometry (GC-EI/TOF-MS, Agilent 6890N24 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed three times with PBS and then mounted in Roti® Mount FluorCare Dapi mounting medium (#HP20.1, Dako). Cells were visualised on a Zeiss LSM700 confocal microscope using ZEN software ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed 3 times 10 min in Tris-Triton Solution and mounted on gelatin-coated slides using Fluorescence Mounting Medium (Dako). Z-stack images (4 optical sections ...
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bioRxiv - Cancer Biology 2020Quote: ... Metabolites were identified and areas integrated based on GC retention time and mass spectra as compared with authentic standards and in conjunction with MSD ChemStation Data Analysis Application (Agilent) using in-house and Wiley metabolite libraries ...
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bioRxiv - Cancer Biology 2020Quote: ... for metabolomics profiling by liquid chromatography-mass spectrometry (LC/MS) using 6550 iFunnel Quadrupole Time of Flight (Q-TOF) mass spectrometer (Agilent). Metabolites were then identified using METLIN database with Mass Profiler Professional software (Agilent) ...
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bioRxiv - Plant Biology 2021Quote: ... together with mass and retention time alignment (0.1 min and 5 ppm respectively) were done in Profinder B.07 software (from Agilent Technologies). Annotation was done based on the ‘find-by-formula’ algorithm ...
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bioRxiv - Neuroscience 2020Quote: ... Experiments were conducted at 100% maximum power output (100V) using custom monophasic waveforms (400μs rise time; Agilent Benchlink Waveform Builder). Mice were restrained using plastic body-contour shape restraint cones (0.5mm thick ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were washed three times with TBS-T and incubated for 1 hour with HRP-conjugated secondary antibodies (Dako). Proteins were detected by enhanced chemiluminescence reagents (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2022Quote: Flow-injection analysis for mass spectrometry-based metabolomics was performed using an Agilent 6550 Series quadrupole time-of-flight mass spectrometer (Agilent) by an adaptation of the method described by Fuhrer et al.7 The analysis was performed utilizing an Agilent 1100 Series HPLC system (Agilent ...
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bioRxiv - Immunology 2022Quote: ... the ELISA plates were washed 3 times with TBS buffer and a rabbit anti-human HRP antibody 1:3000 (Dako) in blocking solution was applied for 1 hour (RT ...
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bioRxiv - Cancer Biology 2019Quote: ... Devices were then washed with 1× PBS solution for 3 times and mounted on glass slides using fluorescent mounting medium (Dako) and the coverslips were sealed using nail polish ...
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bioRxiv - Molecular Biology 2021Quote: ... Then all membranes were washed 3 times and mounted on coverslips using an aqueous fluorescent mounting medium (DakoCytomation, Huddinge, Sweden). Samples were analysed with Leica TCS SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Synthetic Biology 2020Quote: ... Product identity and purity were confirmed by Time-Of-Flight Liquid Chromatography-coupled Mass Spectroscopy (TOF LC-MS, Agilent 6230). Lyophilised samples were resuspended in 32 μl water immediately prior to use.
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bioRxiv - Biochemistry 2021Quote: The plasma metabolome was analyzed by using a 7100 capillary electrophoresis (CE) system coupled to a 6230 time-of-flight mass spectrometer (TOF-MS) from Agilent Technologies equipped with an electrospray ionization (ESI ...
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p97/VCP induces GLI1 to control XBP1-dependent endoplasmic reticulum stress transcriptional responsebioRxiv - Cancer Biology 2021Quote: ... The cells were washed 3 times with PBS for 5 min and then incubated with a secondary antibody: Flex-HRP (EnVision System, Dako) for 30 min and washed 3 times 5 min with PBS ...
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bioRxiv - Biochemistry 2022Quote: Samples were analyzed by FI-MS on an Agilent 6546 Series quadrupole time-of-flight mass spectrometer (Agilent Technologies, USA). The electrospray source was operated in negative and positive ionization mode ...
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bioRxiv - Cell Biology 2022Quote: ... After washing with TBST three times, membranes were exposed to appropriate secondary antibodies (anti-rabbit, anti-mouse, and anti-rat) coupled to HRP (Dako) for 1 hour at room temperature ...
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bioRxiv - Genetics 2022Quote: ... The chromatography peaks were annotated to metabolites according to the retention time and mass spectral information in the Fiehn Metabolomics database (Agilent). The peak heights of representative ions for individual metabolites were normalized by that of internal standard ...
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bioRxiv - Neuroscience 2022Quote: ... the neurons were rinsed three times with blocking buffer and then mounted onto slides using fluorescence mount medium (Agilent, USA). Fluorescence images were acquired as Z-sections at 1 μm × 10 sections with 488 nm (for NTR1 ...
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bioRxiv - Microbiology 2022Quote: Metabolites were analyzed by flow injection into a high-resolution quadrupole time-of-flight (QTOF) mass spectrometer (Agilent QTOF 6546) as described previously (64) ...
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bioRxiv - Plant Biology 2023Quote: The profiles of anthocyanins were analyzed on Agilent 6210 time-of-flight LC/MS (Agilent Technologies, Santa Clara, CA, USA) as described previously (Judd et al ...
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bioRxiv - Immunology 2023Quote: ... the changes in the number of fluorescently labeled bacteria and PMNs in the larvae were recorded by taking photos at different time points with a fluorescence microscope (Agilent BioTek Cytation 5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and coupled to a quadrupole time-of-flight mass spectrometer with a Dual J et Stream Electrospray Ionization (Dual-AJS ESI) source (Agilent Technologies ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... CE-TOFMS and CE-MS/MS analyses were performed using an Agilent CE capillary electrophoresis system equipped with an Agilent 6210 time-of-flight mass spectrometer (Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction was initiated by addition of ATP to 2 mM and 1 mM phopshoenol pyruvate and the change in absorbance at 340 nm over time was monitored with a Neo2 Plate reader (Agilent). Assays were performed at 25°C.
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with 1x PBST for 5 min and incubated with secondary antibodies anti-mouse-HRP (1:10,000) (Agilent Dako, #P0447) and anti-rabbit-HRP (1:10,000 ...
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bioRxiv - Immunology 2023Quote: Real-time oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) were recorded using a Seahorse XFe96 Extracellular Flux Analyzer (Agilent). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... Time-lapse fluorescence images in the head mesenchyme were acquired with 10x magnification on BioTek Lionheart FX Automated Microscope (Agilent). The green and red channels were acquired sequentially with 488-nm laser 561-nm laser ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Sequential drug addition (10-fold dilution) and time-lapse oxygen consumption rate measurements were achieved with a Seahorse XFe96 analyzer (Agilent). Data were analyzed using Wave 2.6.1 software to determine the basal respiration ...
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bioRxiv - Developmental Biology 2023Quote: ... Day 1 and day 8 worms were collected in M9 buffer and washed three times before being pipetted into a Seahorse 96 well plate (Agilent Technologies Seahorse Flux Pack 103793-100) ...
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bioRxiv - Immunology 2023Quote: Real-time oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) were measured using a Seahorse XFe96 Extracellular Flux Analyzer (Agilent). Ex vivo isolated B cells from B-WT and B-Asns mice were stimulated (anti-CD40 at 5μg/ml and IL-4 at 10ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3 times (5 min) in TBST prior to addition of secondary antibody (goat anti-rabbit HRP 1:10,000; Dako - P00448). Blots were washed 3 times in TBST prior to development using SuperSignal West Pico Plus substrate (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... coupled to a high-resolution mass spectrometer with a quadrupole time-of-flight mass analyzer (6550 iFunnel Q-TOF LC/MS; Agilent) with an Agilent Jet Stream (AJS ...
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bioRxiv - Cell Biology 2023Quote: ... All sections were washed three times for 3 min with PBS and mounted with Dako Fluorescent Mounting Medium (Agilent, USA).
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bioRxiv - Microbiology 2023Quote: ... The sample was passed up and down through a 29-gauge needle 6-8 times and the fragment size distribution was determined (∼30 kbp; TapeStation, Agilent).
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bioRxiv - Genetics 2024Quote: ... Day 1 and day 8 worms were collected in M9 buffer and washer three times before being pipetted into a Seahorse 96 well plate (Agilent Technologies Seahorse Flux Pack 103793-100) ...