Labshake search
Citations for Agilent :
1501 - 1550 of 6679 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The conditions for PCR with high-fidelity Pfu DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) were empirically modified (denaturation at 94 °C for 20 s ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with Brilliant II Ultra-Fast SYBR® Green QPCR Master Mix (Agilent) using a Bio-Rad CFX96 Real-Time Detection System ...
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bioRxiv - Synthetic Biology 2019Quote: ... The PCR product was digested with HindIII and XhoI and cloned in pBluescript II SK (Stratagene). The fragment containing hofM ...
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bioRxiv - Genomics 2019Quote: ... PCR products were purified using Ampure XP beads and quantified on an Agilent Bioanalyzer (G2939BA, Agilent) using the High-Sensitivity DNA kit (5067-4626 ...
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bioRxiv - Cancer Biology 2019Quote: ... resuspended in 20 µL of a Herculase II-containing PCR mix (catalog number 600679, Agilent Technologies), and ran for 20 PCR cycles followed by a second 20-cycle PCR using 2 µL of the initial PCR products to add barcodes and P5/P7 Illumina sequencing adapters ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were purified using Ampure XP beads and quantified on an Agilent Bioanalyzer (G2939BA, Agilent) using the High-Sensitivity DNA kit (5067-4626 ...
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bioRxiv - Cancer Biology 2019Quote: ... 1µL of genomic DNA was used for PCR amplification using PfuUltra II Fusion DNA Polymerase (Agilent) (for primers see Table supplement 2 ...
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bioRxiv - Genomics 2021Quote: ... PCR products were purified using Ampure XP beads and quantified on an Agilent Bioanalyzer (G2939BA, Agilent) using the High-Sensitivity DNA kit (5067-4626 ...
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bioRxiv - Genetics 2021Quote: ... an in-out PCR was performed with Herculase II Fusion High-Fidelity DNA Polymerase (Agilent Technologies). The specific size of the PCR products was verified in 1% agarose gel ...
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bioRxiv - Immunology 2022Quote: Synthesized cDNA was then subjected to SMART PCR using Herculase II fusion DNA polymerase (Agilent Technologies) with a common forward primer (5Anchor1-FW1 ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were quantified on a Stratagene Mx3005P qRT-PCR system (Agilent Technologies, Santa Clara, CA, USA) using the KAPA Library Quantification Kit (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... and MAC-seq PreAmp PCR primer and the quality checked on a D5000 Screentape (TapeStation, Agilent). One barcoded library was prepared per plate using TD buffer and TDE1 enzyme (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... the two linear DNA fragments were fused by PCR and cloned into the pBluescript KS(+) (Stratagene), as described previously (32) ...
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bioRxiv - Bioengineering 2022Quote: The targeted loci were amplified from extracted genomic DNA by PCR using Herculase II polymerase (Agilent). PCR amplicons were sequenced using primers ∼200 bp from the expected cut site ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR was employed to measure mtDNA using Applied Biosystems SYBR Green Master Mixes (Agilent Technologies) in a QuantStudio TM 6 Flex Fast Real-Time PCR System ...
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bioRxiv - Bioengineering 2022Quote: The targeted loci were amplified from extracted genomic DNA by PCR using Herculase II polymerase (Agilent). The PCR primers included Illumina sequencing handles as well as replicate-specific barcodes ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNAs were PCR amplified from the genomic DNA using Herculase II Fusion DNA Polymerase (Agilent Technologies) and the flanking primers (hGeCKO_F1 and hGeCKO_R1) ...
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bioRxiv - Microbiology 2024Quote: ... pBRplac derivatives were mutagenized using PCR with mutagenic primers and the Pfu Turbo DNA polymerase (Agilent), followed by DpnI digestion of the template ...
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bioRxiv - Biochemistry 2024Quote: NGS PCR amplicons from RNA guided cleavage reactions were evaluated using D1000 automated electrophoresis (TapeStation; Agilent). Successful plasmid cleavage and PCR amplification produced bands at the ∼180 bp size and were sent for NGS sequencing as previously described3 ...
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bioRxiv - Microbiology 2024Quote: ... The transposon junctions were amplified using a nested PCR protocol using Easy-A cloning enzyme (Agilent) in which the 3’-poly-dC DNA fragments were amplified ...
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bioRxiv - Biochemistry 2019Quote: ... equipped with a 337 nm nitrogen laser or with ESI (electron spray ionization) mass spectrometry on a Q-TOF (quadruple time-of-flight) high-resolution micromass spectrometer (Agilent, Santa Clara, CA, USA) (Figs B to D in S1 file) ...
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bioRxiv - Physiology 2022Quote: ... plasma composition was analyzed by ultra-high-pressure liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF, 1290 Infinity-6540 Agilent Technology, Santa Clara, CA) equipped with electrospray ionization (ESI) ...
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bioRxiv - Immunology 2020Quote: ... The incorporation of 13C-glucose into the TCA cycle metabolites in the intestinal tissue was measured using LC/MS performed on Agilent 6520 high resolution Q-TOF (quadrupole-time of flight instrument) coupled with an Agilent 1200 HPLC system (Agilent Technologies, New Castle, DE), equipped with an electrospray source ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... analyses were carried out using a 6538 UHD model quadrupole time-of-flight mass analyzer equipped with an atmospheric pressure ESI source and a 1260 Infinity model HPLC system (Agilent Technologies, Santa Clara, CA). Samples were analyzed via loop injection with mobile phase composed of 1:1 (v/v ...
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bioRxiv - Bioengineering 2020Quote: Intact mass analysis was performed on a 6230B time of flight (TOF) LC-MS with a 1220 series HPLC (Agilent Technologies, Santa Clara, CA). Mobile phase A consisted of water with 0.1% formic acid ...
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bioRxiv - Genetics 2020Quote: ... and their identities were confirmed using an Agilent 1290 Infinity II liquid chromatography system coupled with Agilent 6530B Quadrupole time-of-flight mass spectrometry (Agilent Technologies, Santa Clara, CA) in a negative ion polarity mode ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The mass spectrometer was operated in positive SIM ion mode (fragmentor 120 V; cycle time 0.6 sec/cycle; Agilent Technologies 6130 Quadrupole LC/MS).
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed in PBS three times for 10 min after each antibody staining and mounted in DAKO fluorescent mounting medium (Agilent Technologies, cat. no. S3023). The following antibodies were used in this study ...
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bioRxiv - Plant Biology 2022Quote: ... analysis was carried out on an Agilent 1290 Infinity series liquid chromatograph coupled with an Agilent 1290 Infinity DAD and Agilent 6530C Accurate Mass quadrupole Time of Flight (qTOF) mass spectrometer (MS) (Agilent Technologies, Santa Clara, USA). Compounds were separated on a Zorbax Extend-C18 Rapid Resolution HT column (2.1 × 50 mm ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Identification of metabolites was performed using commercial standards and by matching their EI mass spectrum and retention time to a metabolomic Fiehn library (from Agilent, Santa Clara, CA, USA) which contains more than 1,400 metabolites ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Metabolomic analysis of the 96 rat liver samples was performed at the Centre for Omic Sciences (COS, Tarragona, Spain) using gas chromatography coupled with quadrupole time-of-flight mass spectrometry (GC-qTOF model 7200, Agilent, Santa Clara, CA, USA). The extraction was performed by adding 400 μL of methanol:water (8:2)-containing internal standard mixture to liver samples (approx ...
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bioRxiv - Synthetic Biology 2023Quote: Samples (3 µL) were injected into an ion mobility drift tube-quadrupole time-of-flight mass spectrometer (LC-IM-QTOF, Agilent 1290 series LC 6560). An Agilent Poroshell 120 HILIC-Z 150 × 2.1 mm ...
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bioRxiv - Systems Biology 2024Quote: ... Mass spectrometry was performed on an Agilent 6530 quadrupole/time-of-flight mass spectrometer (QTOF MS) with a Dual Spray ESI ion source (Agilent Technologies, Santa Clara, CA). Simultaneous MS1 and data dependent MS/MS acquisition was used ...
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bioRxiv - Immunology 2023Quote: Intact mass analysis was performed on a 6520 Accurate Mass Quadrupole Time-of-Flight Mass Spectrometer coupled to an Agilent 1200 Infinity HPLC (Agilent, Santa Clara, CA, USA) using a Jupiter 300 C5 column (2mm*50mm ...
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bioRxiv - Neuroscience 2022Quote: ... Abnormal PrP accumulation was examined using a commercially available ARK (Animal Research Kit)/HRP kit (DAKO) by using the anti-PrP monoclonal antibody 12F10 (1:100 ...
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bioRxiv - Developmental Biology 2023Quote: ... using Agilent RNA 6000 Pico Kit or Agilent RNA 6000 Nano Kit (Agilent, Santa Clara, CA). RNA concentration was determined using Qubit RNA HS Assay Kit (Q32855 ...
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bioRxiv - Cell Biology 2020Quote: ... quantified by performing PCR reaction using Brilliant II SYBR® Green QPCR Master Mix (Agilent Technologies, USA). The primer sequences for PCR analysis were as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA coding TALK1DN was generated by site-directed mutagenesis using PCR and PfuTurbo DNA polymerase (Agilent). The whole cDNAs were sequenced ...
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bioRxiv - Microbiology 2021Quote: ... The quality of the PCR amplicons was assessed using the DNA 1000 microfluidic chip (Agilent Technologies, USA) and the Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Genomics 2020Quote: ... The resulting PCR products were run on an Agilent 2100 Bio-analyzer (Agilent Technologies INC., United States) to verify the expected size (~550bp ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR reactions were performed using standard protocols using EvaGreenTM in the Stratagene Mx3000P system (Agilent Technologies). The relative amounts of mRNAs were calculated from the values of comparative threshold cycle by using Actin as a control ...
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bioRxiv - Microbiology 2022Quote: ... and were used to PCR amplify around the pMMB67EH plasmid using PfuUltra II high-fidelity polymerase (Agilent). PCR products were purified (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: Vpu mutants were generated using PCR-based Quick-change site-directed mutagenesis as per standard protocols (Agilent).The plasmids encoding WT Vpu and Vpu mutants were generated by insertion of the corresponding Vpu fragments from pNL 4-3 ADA proviral constructs into the pCGCG-IRES-GFP plasmid ...
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bioRxiv - Cancer Biology 2019Quote: ... the target region surrounding the expected mutation site was PCR amplified using Herculase II (600675, Agilent Technologies). PCR products were column-purified (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: ... PSD-95 in tissues from the L4-6 spinal cord segments were amplified by PCR (Stratagene M3005p), and a pair of GAPDH primers and Taqman probe were added into each reaction system as the internal reference of PCR amplification ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA libraries were strand selected and PCR amplified for 12 cycles prior to assessment by Agilent Tapestation for quality and Qubit fluorescence assay for quantity ...
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bioRxiv - Microbiology 2020Quote: ... Mutations in the constructs were generated by standard mutagenic PCR using PfuUltra polymerase (QuikChange II; Agilent Technologies).
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bioRxiv - Molecular Biology 2021Quote: ... The genomic region targeted by the CRISPR–Cas9 was amplified by PCR using Turbo Pfu polymerase (Agilent) and the PCR product was cloned into the pCR2.1 TOPO vector (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... RH or IN-deleted constructs were created by invert PCR using Pfu II Taq polymerase (Agilent Technology) with deletion primers (Table S1) ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA libraries were strand selected and PCR amplified for 12 cycles prior to assessment by Agilent Tapestation for quality and Qubit fluorescence assay for amount ...