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Citations for Agilent :
1201 - 1250 of 4607 citations for 7 methyl 4 methylidene 1 propan 2 yl 2 3 4a 5 6 8a hexahydro 1H naphthalene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and heat-induced antigen retrieval was performed using 10mM sodium citrate solution (pH 6.0, pSer129) or citrate Target Retrieval Solution (Dako #S169984-2, pH 6.1) with 0.05% Tween-20 (conformation-specific ...
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bioRxiv - Physiology 2022Quote: ... All assays were run in duplicate under control conditions or in the presence of 5 mM DTT in a 96-well plate and changes in absorbance were tracked using a microplate reader (Synergy 2, BioTek/Agilent, Santa Clara, CA). This concentration was selected based on similar experiments in a previous study [15] ...
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bioRxiv - Immunology 2022Quote: FFPE tissue sections were deparaffinized and antigen retrieval was undertaken in 0.1 M citric acid buffer solution (Dako, target retrieval solution, S169984-2) using an electric pressure cooker (Russell Hobbs RHNHP401) ...
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bioRxiv - Biochemistry 2021Quote: ... and 12 were used as primary detection against T22 capture antibody) was an anti-tau polyclonal rabbit antibody (A002401-2) purchased from Agilent (Santa Clara, CA). A goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen □ retrieval was performed by incubating the tissue for 2 h at 70 °C in Target Retrieval Solution (Dako, Santa Clara, CA, USA) using a 1:10 dilution in demineralized water ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of the plasmid carrying the relevant recombinant provirus was co-transfected with 2 µg of plasmid pMD2.G (a kind gift from D. Trono) and 2 µg of plasmid PVPackgagpol (#217566 from Stratagene, La Jolla, CA) or plasmid pCMV-1′R8.74 (a gift from D ...
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bioRxiv - Genetics 2023Quote: ... Double-stranded cDNA was synthesised from 2 μg of total RNA using AccuScript High Fidelity 1st Strand cDNA Synthesis Kit (Stratagene, La Jolla, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and Cal27 were seeded at a density of 2×104 cells per well in a Seahorse XFe24 Cell Culture Microplate (102340-100; Agilent, Santa Clara, CA). Cells were incubated overnight for adherence in a culture media composed of DMEM and 10% FBS at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The antigen retrieval step was repeated followed by second round staining for pan-CK using anti-pan-cytokeratin antibody (AE1/AE3 mouse monoclonal, Dako-Cat# M351501-2) followed by HRP-conjugated anti-mouse secondary antibody polymer (EnVision+ ...
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bioRxiv - Neuroscience 2023Quote: The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously (Ebina et al. ...
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bioRxiv - Microbiology 2023Quote: ... Plate was shaken continuously at 30°C and OD600 was read every 20 minutes in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent, Santa Clara, CA). MMC (0.5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV plasmid of human synapsin I promoter (hSyn)-tetracycline-controlled transactivator 2 (tTA2) was constructed by subcloning the DNA fragments containing hSyn and tTA2 into pAAV-MCS (Agilent Technologies, CA, USA). The AAV vector was produced as described previously15,35,55 ...
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bioRxiv - Microbiology 2023Quote: ... followed by sample measurements, all performed in duplicates using the OT-2 robot (Opentrons, Long Island City, NY) and BioTek Synergy (Agilent Technologies, Waldbronn, Germany). The acquired data were evaluated using the Shiny application based on the determined standard curves for accurate quantification [62].
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bioRxiv - Microbiology 2024Quote: ... The growth was monitored by measuring the optical density at 600 nm (OD600) in an Epoch 2 plate reader (Agilent, Santa Clara, CA). The growth rates were calculated using the R package growthcurver (32).
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibodies at 4°C overnight: mouse monoclonal anti-CD31 (1:100, JC70A, Dako), rabbit polyclonal anti-VWF (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were incubated overnight at 4°C with the following primary antibodies: mouse anti-BrdU (1:100, Dako), rat anti-BrdU (1:100 Oxford Biotech) ...
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bioRxiv - Immunology 2020Quote: ... FCCP [carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone] (1.5 μM) and Rotenone/Antimycin A (1 μM) (purchased from Agilent Technologies) were injected ...
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bioRxiv - Cancer Biology 2020Quote: Patient-derived explant tissue sections were stained for cytokeratin (DAKO, Cat. M3515; 1:100 overnight at 4°C), α-smooth muscle actin (αSMA ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then incubated overnight at 4 °C with appropriate dilution of anti-Ki67 (1:100, Dako, M7240) in TBS containing Horse Serum (2%) ...
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bioRxiv - Immunology 2023Quote: ... Antigens were tetramerized by incubation at a >4:1 ratio of biotinylated protein with streptavidin-PE (Agilent; PJRS25) or streptavidin-APC (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... The primary incubation was performed overnight at 4°C (phospho-Ser129, BioLegend Cat # 825701, 1:2000; GFAP, DAKO Cat # GA524 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-LMP1 (CS1-4, Dako), anti-LMP2A (MCA2467 ...
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bioRxiv - Molecular Biology 2021Quote: ... MRI of lung was performed with a 7-T Agilent scanner (Agilent, Santa Clara, CA, USA) equipped with a DD2 console and an actively shielded gradient set (205/120 insert of maximum 130 mT m-1 gradient strength) ...
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bioRxiv - Cancer Biology 2020Quote: TP53 IHC was performed using monoclonal anti-TP53 antibody clone DO-7 (Dako, Carpinteria, CA, USA) using standard techniques described elsewhere57 on whole BM biopsy sections in a CLIA-certified laboratory ...
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bioRxiv - Bioengineering 2022Quote: Dynamic release was performed using the 400-DS Apparatus 7 instrument (Agilent Technologies, Santa Clara, CA) at 37 °C in a 10 mL cell ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA with RNA Integrity Number (RIN) > 7 when assessed using the Agilent 2100 Bioanalyzer (Agilent, USA) were selected to move forward for further sequencing.
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bioRxiv - Physiology 2024Quote: MRI studies were performed using a 7-T Agilent/Varian scanner (Agilent, Santa Clara, CA, USA) equipped with a DD2 console ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescent images were obtained on a Keyence BZ-X710 microscope or on a Cytation 7 (Agilent).
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bioRxiv - Evolutionary Biology 2023Quote: ... We quantified sample RNA quantity and quality (RNA integrity number > 7) on a Tapestation 2200 (Agilent) at the University of Montana genomics core ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies diluted in blocking solution were incubated overnight at 4°C (anti-GFAP z0334 DAKO 1:400, anti-Iba1 019-19741 Wako 1:400). After three washes in PBS ...
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bioRxiv - Physiology 2019Quote: ... tissues were incubated with primary antibody over night at 4°C (guinea pig anti-insulin at 1:750, DAKO; diluted in 1% goat serum/PBS). This was followed by 1-hour incubation with fluorophore-conjugated secondary antibodies (Cy3-anti-guinea pig ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentration was measured by Nanodrop and 1 to 3 μg of RNA was reverse transcribed with AffinityScript Multi-Temp RT (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... an optimal mutation rate (0.3–1 base/kb) for 1µg of template was adopted as recommended in GeneMorph II Random Mutagenesis kit (Agilent Technologies). The PCR products were then digested with EcoRI and HindIII and ligated into the pJF118EH vector using the same restriction enzymes ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subjected to antigen retrieval antigen retrieval (pH = 6.0 for cleaved caspase-3 and pH = 9.0 for cleaved PARP-1) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Immunology 2022Quote: ... the ELISA plates were washed 3 times with TBS buffer and a rabbit anti-human HRP antibody 1:3000 (Dako) in blocking solution was applied for 1 hour (RT ...
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bioRxiv - Cancer Biology 2019Quote: ... Devices were then washed with 1× PBS solution for 3 times and mounted on glass slides using fluorescent mounting medium (Dako) and the coverslips were sealed using nail polish ...
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bioRxiv - Neuroscience 2021Quote: ... free floating sections were blocked with 3% donkey serum and incubated overnight with either rabbit anti-GFAP (1:10000, Dako), rat anti-CD68 (1:200 ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were incubated in the following primary antibodies for 3 h at 24L: gastrin (1:1000, Cat #A0568, A0568, AB_2757270, Dako Agilent Technologies), Chromogranin A (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous peroxidases were blocked by incubation with 3 % H2O2 and then blocked for 1 h (Dako Serum-free protein block). Sections were then incubated with the 1st primary antibody (FABP7 ...
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bioRxiv - Immunology 2024Quote: ... residues D141 of Regnase-1 and D252 of Regnase-3 were mutated to asparigines using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 5[μm column (Agilent).The mobile phases were MS solvent A (H2O ...
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bioRxiv - Biochemistry 2021Quote: ... Antigen retrieval using a standard pH 6 sodium citrate buffer (BioGenex) was performed and sections were stained with Monoclonal Mouse Anti-Human CD45 (Dako, M0701, dilution 1:200) using the M.O.M ...
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bioRxiv - Plant Biology 2023Quote: ... Lipid bands were scratched from the plates and their fatty acids extracted (fatty acid methyl esters FAMEs) and quantified by GC-MS (Agilent 7890 A and MSD 5975 Agilent EI) as in (39) ...
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bioRxiv - Cell Biology 2024Quote: ... Step 3 was endogenous peroxidase blocking (Dako REAL Peroxidase-blocking reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... 3′ - Diaminobenzidine (DAB+) solution (DakoCytomation, Glostrup, Denmark), and counterstained by Harris hematoxylin.
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How conspicuous are peacock eyespots and other colorful feathers in the eyes of mammalian predators?bioRxiv - Zoology 2019Quote: ... Samples were illuminated by an Ocean Optics PX-2 Pulsed Xenon Light source triggered at 20 Hz using square wave pulses from a model 330120A function generator (Agilent Technologies Wilmington, DE, USA); the source was turned on and allowed to warm up and stabilize for 15 minutes before data collection ...
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bioRxiv - Microbiology 2021Quote: ... The R682S furin cleavage mutation was introduced into the SARS-CoV-2 S expression plasmid by QuikChange site-directed mutagenesis (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of HEK293T cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described (104) ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of 293 cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described38 ...