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Citations for Agilent :
1151 - 1200 of 1254 citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... the slides were treated with 1:10 normal goat serum in PBS for 15 minutes and incubated for 60 minutes with 1:200 diluted Rabbit anti-human thyroglobulin (Dako, Denmark) or 1:400 diluted Rabbit anti-human calcitonin (Dako ...
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bioRxiv - Cell Biology 2020Quote: To identify the causative mutation in these families we sequenced the whole exome of the affected individuals with the SureSelect human AllExon 50Mb kit (Agilent Technologies) and sequenced on the HiSeq 2500 (Illumina ...
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bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... RNAs from 4 different experiments (biological quadruplicate) were extracted as described above and analyzed on SurePrint G3 Human Gene Expression 8×60K v2 Microarrays (#G4851B, Agilent Technologies) as previously described (36) ...
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bioRxiv - Neuroscience 2020Quote: ... Human α7-nAChR R208I-GFP and E211N-GFP were generated from pcDNA3.1-CHRNA7-mGFP and human α3-nAChR I284R-GFP and N287E-GFP were generated from human α3-nAChR-GFP by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Serum samples were depleted of the top 14 interfering high-abundant proteins using the Multiple Affinity Removal Column Human 14 (Agilent, USA). Then ...
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bioRxiv - Immunology 2021Quote: ... Exome sequencing was performed on genomic DNA from Patients 1 and 2 and their parents using a SureSelect Human All Exon kit (Agilent Technologies) for targeted enrichment ...
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bioRxiv - Neuroscience 2021Quote: The optimal concentration was determined for each antibody: CD3 (polyclonal rabbit – anti-human CD3 IgG, cat. no. A045201-2, Agilent Technologies), 1:60 ...
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bioRxiv - Biophysics 2021Quote: Mutations were introduced into the human CFTR(E1371S)/pGEMHE and CFTR(D1370N)/pGEMHE sequences using the QuikChange II XL Kit (Agilent Technologies) and confirmed by automated sequencing (LGC Genomics GmbH) ...
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bioRxiv - Cell Biology 2020Quote: ... All hESC lines were analysed for their genetic content by array-based comparative genomic hybridization (Human Genome CGH Microarray 4×44K, Agilent Technologies) as previously described (Jacobs et al. ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated for 45 minutes in 1% dried skim milk in PBS-T with rabbit anti-human C1q (1:300, Dako, A0136) and HRP-conjugated goat anti-rabbit IgG (1:10.000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The library was then enriched by liquid phase hybridization using Agilent SureSelect XT Human All Exon v6 (Agilent Technologies, CA, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... hepaticus versus the tdTomato fluorescent protein (72 h transduction) was quantified in epithelial intestinal HT-29 cells using the Human GE 4x44K v2 Microarray Kit (Agilent Technologies) as we previously detailed [13 ...
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bioRxiv - Microbiology 2022Quote: ... Slides were then washed in PBS/0.05% Tween 20 and incubated with rabbit anti human VWF (A008229-2, DAKO/Agilent tech) 1/500 or rabbit IgG isotype control (AB-105-C ...
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bioRxiv - Microbiology 2023Quote: ... all tissue section slides were treated with a mouse monoclonal Anti-Human IgG p27KIP1 (Clone SX53G8.5 dilution 1:100; Code M7203; Dako Cytomation, Denmark) that cross-reacts with p27kip1 using a protocol described previously [24] ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-CD61 immunohistochemistry (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:75, Clone Y2/51, Dako Omnis, Code Number: M0753) was also employed to evaluate the presence of platelet-enriched microthrombi ...
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bioRxiv - Cancer Biology 2022Quote: Genome-wide copy number variations were assessed using the SurePrint G3 Human aCGH 4×180K Microarray (Agilent Technologies, Santa Clara, CA), according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was fragmented into 180–280 bp by sonication and subjected to library preparation using the Agilent SureSelect Human All Exon V6 Kit (#5190-8864, Agilent Technologies). The enriched libraries underwent paired-end 150bp sequencing on the Illumina HiSeq 2000 platform.
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of the library was hybridized to the Agilent SureSelect XT Human All Exon V4 probes (Agilent Technologies 5190-4634). The targeted exon fragments were captured by Streptavidin beads (Fisher 65602) ...
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bioRxiv - Biophysics 2024Quote: The recombinant human Cα subunit of cAMP-dependent protein kinase with the Phe to Ala mutation in position 100 (PKA-CF100A) was generated from the human PKA-Cα wild-type using Quik-Change Lightning mutagenesis kit (Agilent genomics). The key resource table lists the PCR primers used to modify the pET-28a expression vector encoding for the wild-type human PKA-Cα gene (PRKACA – uniprot P17612 ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using a low-input QuickAmp labeling kit and human SurePrint G3 8×60K microarrays (Agilent Technologies), as previously described (Sulpice et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Flag-tagged wt and Ex19Del human EGFR constructs in the lentiviral VIRSP vector were generated by PCR with Herculase II Fusion DNA Polymerase (Agilent technologies) from EGFR WT (a gift from Matthew Meyerson ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8□×□60 K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Pathology 2023Quote: ... with the mouse Prnp encoding the 3F4 epitope (109M, 112M human numbering) was used as a template for site-directed mutagenesis (QuikChange Site Directed Mutagenesis kit™) (Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... Samples from the primary human monolayer experiment were fixed and immunostained for insulin with guinea pig anti-insulin primary antibody (Dako A0564) and Alexa-Fluor 568 goat anti-guinea pig secondary antibody (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Deletions of SP1 sites on human MGMT promoter were performed using Quick-Change XL Site-Directed Mutagenesis kit (Agilent Technologies, USA).34 The mutated promoters were further cloned into pGL3 basic vector ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Cell Biology 2024Quote: Cy3-labeled cRNAs were further hybridized onto Agilent Whole Human Genome kit 4 x 44K slides (Catalog number G4112F, Agilent Technologies) containing 44,397 oligonucleotide probes in 2X Hi-RPM Hybridization buffer (Catalog number 5188-5242 ...
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bioRxiv - Neuroscience 2024Quote: ... Then membranes were probed with the following antibodies: anti-human tau polyclonal antiserum KJ9A (1:5,000; A0024, Dako Cooperation, Carpinteria, CA) and rabbit anti-GAPDH antiserum (1:5,000 ...
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bioRxiv - Neuroscience 2024Quote: The mutant PDI-Ala256 protein was prepared from the full-length wild-type human PDI cDNA using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). For protein purification ...
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bioRxiv - Cell Biology 2024Quote: ... Mutant human PINK1 constructs were generated by QuickChange XL site-directed mutagenesis according to the manufacturer’s guidelines (Agilent Technologies #HPA 200516). The following constructs were made ...
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bioRxiv - Molecular Biology 2024Quote: pB-T-PAF-VLDLR(1-797)Δ(751-779)-C-tag was obtained by PCR amplification of VLDLR from human placenta cDNA (Edge Biosystems) using Herculase-II Fusion DNA polymerase (Agilent Technologies), followed by Gibson assembly with pB-T-PAF using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: ... was used for the measurement of OCR of primary human RPTECs seeded on 96-well XF96 Cell Culture Microplates (#101085-004, Agilent Technologies) at 5,000–7,000 cells per well depending on the experiment ...
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bioRxiv - Immunology 2024Quote: ... the formalin-fixed paraffin-embedded human PDAC tissue sections were deparaffinized by Xylene and gradient alcohol solutions and stained with hematoxylin (S3301, Dako, US) for 1 min ...
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bioRxiv - Molecular Biology 2024Quote: ... After incubation the cells were fixed in 4 % PFA and stained with rabbit anti human C3c FITC (1: 100 Dako F020102) antibody ...
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bioRxiv - Cancer Biology 2024Quote: Array-CGH experiments were carried out using 400K human genome CGH microarray and standard Agilent protocols (Agilent Technologies, Santa Clara, CA). Commercial human genomic DNA (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... labelled reference and tumour DNA were mixed as probes and hybridized onto an Agilent 400K human genome CGH microarray (Agilent Technologies) for 40 hours ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with prediluted antibody polyclonal Guinea Pig anti-Human Insulin (cat. IR002 - Agilent Technologies, Santa Clara, CA, USA) for 1h at RT ...
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bioRxiv - Genetics 2021Quote: ... and 5081) were used for whole-exome sequencing prepared using the SureSelect Human All Exon V7 probe set (Agilent, Design ID S31285117) per the manufacturers protocol ...
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bioRxiv - Neuroscience 2021Quote: ... RNA qualities of the human RNA samples (RNA Integrity Number; RIN score) were assayed with high sensitivity RNA Screen Tape (Agilent 5057-5579) and shown in Table S4 ...
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bioRxiv - Immunology 2021Quote: ... and bound serum IgG was detected by 70 µL/well of 1:3000 diluted rabbit anti-human IgG antibody linked to horseradish peroxidase (Agilent, P021402-2) incubated for 2h at RT ...
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bioRxiv - Genomics 2021Quote: ... Each sample was prepared according to Agilent library preparation protocols (Human All Exon 50 Mb kit; Agilent Technologies, Santa Clara, CA, USA). Libraries were subjected to paired-end sequencing on an Illumina Hiseq 2000 and 2500 instrument (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Point mutations were introduced into the wild-type human Myo1e in the pEGFP-C1 vector using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies #210519) [7 ...
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bioRxiv - Immunology 2021Quote: ... Paraffin sections (4µm thick) of FFPE thymus and lymph node tissues (NMR, mouse, and human control) were stained for cytokeratin (AE1/AE3, Dako GA05361-2) on a Dako Omnis autostainer with pressure cooker antigen retrieval (TrisEDTA ...
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bioRxiv - Cell Biology 2021Quote: ... Entry clones for other mutant dynamin 2 were prepared by introducing corresponding mutations into the Entry clone of wild type human dynamin 2 using QuikChange Lightning Site-directed Mutagenesis kit (Agilent Technologies, 210518) following manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... Synthesis of cDNA from isolated RNA as well as a Universal Human Reference RNA that was used as negative control or for normalization (HREF, Stratagene/Agilent # 740000) was performed using an oligo-dT primed SuperScript III Reverse Transcriptase (Invitrogen ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: Mutagenesis of His 359 and Glu 363 to Alanine in human MYC (MycHEA mutant) was performed using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies # 200519), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: The hydrophilic N-terminal region of CLN6 was screened against a human fetal brain library using Stratagene’s Cytotrap yeast two-hybrid system (Y2H) as previously published (Stratagene, La Jolla, CA). Briefly ...