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Citations for Agilent :
851 - 900 of 1254 citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Anti-Fgb (rabbit) and anti-human Pecam1 (mouse) were from Dako (Carpinteria, CA, USA). Anti-Alb (sheep ...
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bioRxiv - Immunology 2022Quote: ... tissue sections were firstly stained with monoclonal anti-human CD45 (DAKO, 2B11+PD7/26), CD20 (DAKO ...
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bioRxiv - Immunology 2020Quote: ... which were then hybridized to SurePrint G3 Human Gene Expression 60K GeneChip microarrays (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... or IgA (polyclonal rabbit anti-human IgA/HRP, Dako, P0216, at 1:2000 dilution) was added for 1 hr at room temperature and the enzyme reaction was developed with TMB plus (Kementec ...
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bioRxiv - Systems Biology 2023Quote: ... 50 Mb targeted exons were captured using SureSelect Human All Exon V5 (Agilent Technologies). Hundred bp paired-end sequence reads of the captured exons were generated using HiSeq 2000 Sequencing System (Illumina ...
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bioRxiv - Biophysics 2023Quote: Human FKBP12.6 cDNA was modified by site directed mutagenesis (QuikChange Lightning kit; Agilent Technologies) to introduce one of eight single cysteine mutants (G1C ...
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bioRxiv - Microbiology 2024Quote: ... for 24 h or by 15 µg/ml α-human IgG (Agilent #A042301-2) for 48 h ...
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bioRxiv - Immunology 2023Quote: ... Antigen-specific IgG was detected using rabbit anti-human IgG HRP (Dako, Glostrup, Denmark). ELISA plates were developed using TMB solution (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Library preparation was performed by using SureSelectXT Human All Exon V5 (Agilent, 5190–6209) according to the instructions ...
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bioRxiv - Cell Biology 2023Quote: ... DNA library hybridization was performed by using Agilent SureSelect Human All Exon V6 (Agilent). Paired-end sequencing (150 bp ...
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bioRxiv - Immunology 2022Quote: ... Antigen-specific IgG was detected using rabbit anti-human IgG HRP (Dako, Glostrup, Denmark). ELISA plates were developed using TMB solution (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... Exome capturing was performed using SureSelect Human All Exon in-solution capture reagents (Agilent). In case RNA was pooled in for sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... Calibrator cDNA was transcribed from Quantitative PCR Human Reference Total RNA (Agilent Technologies, USA). Relative gene expression was calculated upon normalisation to two reference genes ...
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bioRxiv - Neuroscience 2024Quote: Human tissues were immunostained with primary antibody diluted in DAKO antibody diluent (DAKO S0809) and stored in a humidified chamber at 4 °C for 2-3 days ...
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bioRxiv - Neuroscience 2024Quote: ... Exome capture libraries were generated using the SureSelect Human All Exon v8 kit (Agilent). Paired-end sequencing was performed at 101 base pairs on each side of the DNA fragment on the Illumina NovaSeq 6000 platform ...
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bioRxiv - Genomics 2024Quote: ... mouse IgG1 anti-human CK 19 (clone: RCK108, GA61561-2, Dako, Carpinteria, CA, USA), and mouse EpCAM ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-human leukocyte antigen (HLA)-DR (1:200 Agilent, M0746, TAL.1B5 clone) in blocking solution overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to synthesize cDNA from 1 ug RNA and Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) was used for the Quantitative RT-PCR reactions ...
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bioRxiv - Molecular Biology 2021Quote: ... the immunoprecipitated DNA and total DNA were quantified by Power SYBR Green PCR Master Mix in a Mx3000P Real-Time qPCR System (Agilent Technologies). The primers used for qPCR are listed in Sup ...
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bioRxiv - Plant Biology 2020Quote: ... RT-PCR was performed with 1μL of diluted cDNA (1:9) in a Brillant III ultra fast SYBRgreen QPCR master mix (Agilent Technologies, USA) using a StepOnePlus Real-Time PCR System (Applied biosystems ...
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bioRxiv - Genomics 2021Quote: ... Final libraries were evaluated using fluorescent-based assays including qPCR with the Universal KAPA Library Quantification Kit and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100). Libraries were sequenced on an Illumina NovaSeq 6000 system using 2 x 150bp cycles.
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was carried out using Thunderbird SYBR qPCR Mix (Toyobo, Japan) with a Stratagene mx 3000p real-time thermal cycler (Agilent, USA). Relative transcript levels were calculated against a standard curve with normalization to the expression of PLANT U-BOX PROTEIN1 (PUB1 ...
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bioRxiv - Microbiology 2021Quote: ... ChIP efficiency was assessed by qPCR using SensiMix SYBR master mix using a Stratagene Mx3005P (Agilent Technologies, Santa Clara, CA, USA). Primer sequences for ChIP experiments are shown in Table 1 ...
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bioRxiv - Immunology 2021Quote: ... HES5 and GATA6 genes were made for 3 replicates using the Brilliant III Ultra-Fast SYBR green qPCR master mix (Agilent Technologies) and analyzed on a CFX Opus Real-Time PCR system (BioRad ...
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bioRxiv - Immunology 2022Quote: The thermal stability assay was performed to detect the exposed hydrophobic residues by an MX3005 qPCR instrument (Agilent, Santa Clara, USA) with SYPRO Red (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... The qPCR assays were carried out in a MX 3005 real-time PCR system (Agilent, Santa Clara, CA 95051, United States) using Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent) ...
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bioRxiv - Plant Biology 2021Quote: ... USA) using gene specific oligonucleotide (Supplemental table S3) and Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies, USA). Relative gene expressions were calculated using 2−ΔΔCt method ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed with 4 μl Brilliant III Ultra-Fast SYBR QPCR as instructed by the manufacturer (Agilent Santa Clara). All primers for quantification of either expression of viral m/cRNAs or antiviral responses are shown below.
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bioRxiv - Molecular Biology 2021Quote: The qPCR was run on an MxPRO3005 thermal cycler and analysed from endpoint scatter plots using MxPRO software (Aligent technologies, Stratagene, USA). The qPCR cycling conditions for both L1014F and L1014S were 95°C for 10 minutes ...
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bioRxiv - Pathology 2020Quote: ... and real-time RT-PCR was performed using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies, CA, USA). The synthetic gene-specific primers are listed in Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent-based assays including qPCR with the Universal KAPA Library Quantification Kit and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100) were used to evaluate final libraries ...
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bioRxiv - Genetics 2020Quote: ... Raw qPCR fluorescence data were collected and analyzed by the default settings of the MxPro software v.4.10 (Agilent Technologies, USA). Amplification efficiencies for each primer pair were determined using the Ct (threshold ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed on 1:10 diluted cDNA using either SYBR-containing Brilliant III Ultra-Fast QPCR Master Mix (Agilent Technologies) and gene-specific primers ...
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bioRxiv - Immunology 2023Quote: ... The Pfr2 copy number within each 20ng DNA skin sample was calculated from the standard curve using the ARIAMx qPCR software (Agilent, USA) as a proxy for estimated trypanosome load ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Real time quantification of mRNA levels of the genes of interest was performed using Brilliant II SYBR Green QPCR Master Mix (Agilent Technologies) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Bevacizumab-sensitive/resistant U87 cells were cultured identically to wild-type U87 cells.79 Cells were screened for mycoplasma every 3 – 4 months with the MycoSensor qPCR Assay Kit (Agilent Technologies).
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bioRxiv - Developmental Biology 2023Quote: ... Gene expression levels were determined using the Brilliant III Ultra-Fast SYBR® Green QPCR master mix (Agilent, Santa Clara, USA) and Agilent AriaMx Real-Time PCR system (Agilent ...
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bioRxiv - Genomics 2023Quote: ... Real time quantification of mRNA levels of the genes of interest was performed using Brilliant II SYBR Green QPCR Master Mix (Agilent Technologies) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... for Illumina Sequencing platforms according to the qPCR Quantification Protocol Guide and qualified using the TapeStation D1000 ScreenTape (#5067-5582, Agilent Technologies). Indexed libraries were then submitted to an Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR analysis was performed using Perfect Start Green qPCR Super Mix (+DyeII) (AQ602-24, TransGen, Beijing, China) and the AriaMx Real-Time PCR System (Agilent Technologies). See Table S2 for primer information.
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bioRxiv - Cell Biology 2023Quote: ... fluorescent-based assays were used including qPCR with the Universal KAPA Library Quantification Kit and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... 5ng of cDNA was used for the qRT-PCR reaction using Brilliant III Ultrafast SYBR Green QPCR master mix (Agilent Technologies) on AriaMx qRT-PCR system (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... and RT-qPCR was performed using the 1-Step Brilliant II SYBR Green quantitative RT-PCR master mix kit (Agilent Technologies) on a Bio-Rad C1000 Thermal Cycler CFX96 Real-Time System ...
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bioRxiv - Microbiology 2024Quote: ... Each pool was quantified using qPCR (KapaBiosystems, #KK4854) and sized using the Agilent Bioanalyzer high-sensitivity DNA kit (Agilent, #5067-4642). The Illumina MiSeq platform with the MiSeq Reagent 222 kit v2 (#MS-102-2003 ...
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bioRxiv - Neuroscience 2024Quote: ... Final library quantity was measured by KAPA SYBR® FAST qPCR and library quality evaluated by TapeStation D1000 ScreenTape (Agilent Technologies). Equimolar pooling of libraries was performed based on QC values and sequenced on an Illumina NovaSeq S4 (Illumina ...
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bioRxiv - Biochemistry 2022Quote: Fusion constructs and large deletions were generated by overhang extension PCR [79] using primers from IDT (IDT, Coralville, IA) and cloned Pfu DNA polymerase (Stratagene, La Jolla, CA). Point mutations and small deletions or insertions were generated using QuickChange [80] with Turbo Pfu DNA polymerase (Stratagene ...
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bioRxiv - Pathology 2020Quote: ... beads were collected for 2 min on a magnet and then resuspended in 25 µL PCR master mix containing PCR1 primers and Herculase-II (Agilent, Santa Clara CA). PCR cycling was as follows ...
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bioRxiv - Plant Biology 2021Quote: ... The mutated variants of AopW1 were generated by inserting mutations in the corresponding primers in combination with the Quick Change Lightening Site Directed Mutagenesis kit (Agilent Technologies, SC, USA). For this purpose ...
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bioRxiv - Molecular Biology 2024Quote: ... and the quality as well as yield of fully-barcoded DNA primers was evaluated with total RNA Pico Assay (Agilent Technologies, 5067-1513) and by fluorescent in situ hybridization (FISH ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... 639KKRRRPSALEADSPMAPKR657 were mutated sequentially to alanine with the NLS1a and NLS1b primer sets listed in Table S8 using the QuikChange site-directed mutagenesis system (Agilent, San Diego, CA) to create pET24b(+)-YPet-Kif18B(605-828)-NLS1 ...