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Citations for Agilent :
1101 - 1150 of 6761 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Sizes and yields of RACE products (2 µL aliquots) were determined using a Fragment Analyzer (Advanced Analytics; now Agilent, Santa Clara USA) equipped with 55 cm electrophoresis capillaries and reagents capable of resolving dsDNA fragments between 35 and 1500 bp (see Fig 1E) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the tissue was soaked in 200μl dichloromethane containing 200ng 2-tetradecyl acetate (internal standard) in 2ml glass vials with PTFE-coated caps (Agilent, Santa Clara, USA) for one hour ...
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bioRxiv - Neuroscience 2021Quote: ... the co-cultures were incubated first with primary antibody for glial fibrillary acidic protein (GFAP, 1:400, Z033429-2, Dako, Glostrup, Denmark) prepared in 5% NGS in DPBS overnight at +4 following an incubation with Alexa Fluor405 (A31556 ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were washed with TBST and slides were developed by adding AEC+ High Sensitivity Substrate Chromogen Ready to use (Dako K346111-2).
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bioRxiv - Developmental Biology 2020Quote: ... and incubated for 30 minutes at room temperature with the secondary antibody (Supplementary Table 2)(1:500 diluted in DAKO Antibody Diluent). Cells were washed with 0.2% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... three washes with phosphate buffered saline (PBS) and the incubation with the primary antibody in the DAKO Real TM antibody diluent (Agilent #S202230-2) for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Sialyl-Lewis a and Sialyl-Lewis x (sLex also known as CD15s) were detected with 2 μg/ml of mAbs NS-1116-19.9 (Dako, Agilent Technologies, USA) and CSLEX1 (Becton Dickinson ...
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bioRxiv - Genomics 2019Quote: ... Gene expression was performed on 7900 HT fast real time PCR system (Applied Biosystem, USA) using 2× Brilliant III SYBR@ Green qPCR Master Mix (Agilent Technologies, USA) and gene specific primers (Supplementary Table 1) ...
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bioRxiv - Immunology 2021Quote: ... The membranes were washed and incubated with rabbit anti-goat IgG (500 ng ml−1, 5% milk in PBST; Dako, P044901-2), rabbit anti-mouse IgG (1.3 μg ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... were designed based on the DNA sequence for SARS-CoV-2 Wuhan-Hu-1 using the QuickChange Primer Design tool (Agilent Technologies, Inc.). Mutagenesis was carried out on a pCDNA-SARs2 Wuhan-Hu 1 S plasmid to create the P681H mutation ...
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bioRxiv - Genomics 2021Quote: ... After 10 minutes of formalin fixation of the tissue on the slides they were dried with isopropanol and stained with Mayer’s hematoxylin (Dako, cat.no.: S330930-2) and bluing buffer (Dako ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were rinsed in PBS (2 × 10 min) before antigen-retrieval by incubation in target retrieval solution (Dako S1700, Glostrup, Denmark) for 40 min at 85 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-SΔ19 expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) by following the manufacturer’s instructions and using mutagenic oligonucleotides SaCoV2-K417T-F ...
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bioRxiv - Immunology 2023Quote: ... following standard protocol and plated at a concentration of 2×105 cells per well of a seahorse XFe96 assay plate in seahorse XF DMEM media (Agilent; 103575-100) supplemented with 1mM pyruvate ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor xenograft slides were incubated at 60 °C for 2 h followed by antigen retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Synthetic Biology 2023Quote: ... 200 μL of supernatant was pipetted into a nylon syringeless filter (Whatman, UN203NPENYL) and transferred to a 2 mL chromatography vial (Agilent, 5181-3376) with a glass vial insert (5183-2085 ...
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bioRxiv - Bioengineering 2022Quote: ... Incubation with secondary antibodies diluted in blocking solution was performed 1 h at RT: anti-rabbit-IgG conjugated to horseradish peroxidase (1:10,000, cat. no. P044801-2; Dako, Carpinteria, CA, USA), anti-mouse-IgG conjugated to horseradish peroxidase (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... 200 mm by 2 mm fitted with a precolumn 10 mm by 2 mm (Dr. Maisch GmbH, Ammerbuch, Germany) coupled to an Agilent LC/MSD Ultra Trap System XCT 6330 (Agilent, Waldbronn, Germany). For analysis were used ...
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bioRxiv - Neuroscience 2023Quote: ... samples were centrifuged at 20.800 xg for 2 min and 100 µl sample was injected on a SEC-HPLC column (Bio SEC-3 300 Å, Agilent, USA) using an Agilent 1260 Infinity II system (Agilent ...
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bioRxiv - Cell Biology 2024Quote: qPCR was performed using 2× SYBR Green qPCR Mix (Low ROX) (Aidlab Biotechnologies, Beijing, Chian) in a Stratagene Mx3000P (Agilent Technologies, SUA). With GAPDH mRNA as endogenous control ...
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bioRxiv - Microbiology 2024Quote: ... 1:2 dilution series in serum-free media was performed and cells were infected in a 96-well plate (Agilent, 204624-100) for 1 hour at 37°C followed by addition of methylcellulose (Sigma M0512-500G ...
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bioRxiv - Neuroscience 2024Quote: ... Wildtype mouse brain paraffin sections were used for IHC analysis with a dilution of mouse serum at 1:200 with antibody diluent solution (DAKO, S080983-2). Mouse anti-NMDAR1 monoclonal antibody (BD ...
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bioRxiv - Biochemistry 2021Quote: ... Cross-linked peptides were enriched with Fe(III)-NTA cartridges (Agilent Technologies; Santa Clara, Ca) using the AssayMAP Bravo Platform (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... and the peptides desalted and concentrated upon C18 resin (OMIX C18 pipette tips; Agilent #A57003100) essentially as previously described (69) ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were expressed in BL21-Codon Plus (DE3) RIPL cells (Agilent, Santa Clara, CA, USA) in ZYP-5052 auto-induction media(Studier ...
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bioRxiv - Systems Biology 2021Quote: ... Reconstituted peptides were separated using an Infinity 1290 UPLC system (Agilent, Palo Alto, CA, USA), and peptides were detected using a triple-quadrupole G6495 mass spectrometer (Agilent ...
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bioRxiv - Physiology 2022Quote: ... The sequential separation of peptides on trap column (100 μm × 20 mm, RP-C18, Agilent) and analysis column (75 μm × 150 mm ...
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bioRxiv - Biophysics 2019Quote: ... while labeled and unlabeled peptide were separated by reverse-phase HPLC (Agilent, Santa Clara, CA). The purity of the conjugations was confirmed by MALDI-TOF (Bruker ...
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bioRxiv - Biochemistry 2019Quote: ... peptides were purified and concentrated using OMIX Tips C18 reverse-phase resin (Agilent Technologies Inc.) according to the manufacturer’s specifications ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were acidified with 1% TFA and purified on Omix C18 tips (Agilent, catnr. A57003100), dried and re-dissolved in 20 µl loading solvent (0.1% TFA in water/acetonitrile (98:2 ...
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bioRxiv - Biophysics 2021Quote: ... Approximately 300 fmol of peptides was separated and analyzed using the HPLC system (Agilent Technologies) coupled on-line to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were injected onto a SB-C18 Nano HPLC column (0.5 × 150 mm, Agilent Zorbax), and resolved using a 120min gradient from 5-45% acetonitrile in 0.1% formic acid ...
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bioRxiv - Microbiology 2022Quote: ... desalted with the AssayMAP Bravo robot using C18 column and AssayMAP Peptide Cleanup Protocol (Agilent). All samples were dried in a Speed-Vac and peptides were resuspended in 2 % ACN ...
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bioRxiv - Plant Biology 2023Quote: ... Analyses were conducted in an AdvanceBio Peptide Map column (2.1×100mm, 2.7micron, Agilent Technologies, Inc.) and Infinity II 1290 UHPLC coupled to a 6550 iFunnel QTof (Agilent Technologies ...
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bioRxiv - Biophysics 2022Quote: ... The labeled peptide was purified by reverse-phase chromatography using ZORBAX 300SB-C18 column (Agilent) equilibrated with 5% acetonitrile ...
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bioRxiv - Systems Biology 2023Quote: ... 50 µg of peptide material was injected for fractionation by RP-HPLC (Agilent series 1200) connected to a Probot fractionator (LC Packings) ...
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bioRxiv - Microbiology 2023Quote: Peptide concentrations were determined using a Take3 micro-volume plate (Agilent Technologies, Santa Clara, CA) before labeling each sample with the appropriate TMT10-plex reagent ...
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bioRxiv - Plant Biology 2023Quote: ... The hydrolysates were then loaded into Zorbax 300SB-C18 peptide traps (Agilent Technologies, Wilmington, DE) and analyzed by a Q Exactive mass spectrometer (Thermo Fisher) ...
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bioRxiv - Systems Biology 2023Quote: ... the peptides were reconstituted in 10 mM TEAB and fractionated using a bRPLC column (Agilent 300 Extend-C18 column ...
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bioRxiv - Evolutionary Biology 2024Quote: ... peptides were purified and concentrated using OMIX Tips C18 reverse-phase resin (Agilent Technologies Inc.) according to the manufacturer’s specifications ...
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bioRxiv - Microbiology 2024Quote: Peptide desalting was carried out using OMIX C18 solid-phase extraction (SPE) tips from Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3X with TBST followed by incubation with HRP-conjugated secondary antibodies (Rabbit Cytiva/Amersham NA934; Mouse Agilent/Dako P026002-2) for one hour at room temperature ...
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bioRxiv - Genetics 2020Quote: ... and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab, Santa Clara, CA, USA). The hexane was evaporated under a nitrogen gas flow ...
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bioRxiv - Cell Biology 2020Quote: ... RNA quality of all samples (n=7 time points and n=3 experiments from independent passages) was evaluated by both Nanodrop for (A260/280 >2) and Bioanalyzer 2100 High Sensitivity RNA Analysis chips (Agilent, Cat. 5067-1513) which displayed intact RNA integrity (RIN >9) ...
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence microscopy was performed on de-paraffinized tissue sections that received heat-induced antigen retrieval using Target Retrieval Solution (Agilent Dako, S169984-2). After washing and permeabilization in TBS-T universal buffer (0.2% Triton X-100 in tris-buffered saline) ...
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bioRxiv - Genomics 2020Quote: ... a plasmid encoding the replicases of AAV serotype 2 and the capsid of AAV serotype 9) and pHelper (Agilent Technologies, lab reference pZMB0088) which provides AAV adenoviral helper functions ...
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bioRxiv - Biochemistry 2019Quote: ... in 200μl of dichloromethane containing 200 ng of 2-tetradecyl acetate (internal standard) in 2ml glass vials with polytetrafluoroethylene-coated caps (Agilent, Santa Clara, California). After one hour ...
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bioRxiv - Biochemistry 2019Quote: ... solvent A) and methanol:isopropanol (8:2 v/v, solvent B) was generated by an Infinity II 1290 UPLC (Agilent, Santa Clara, CA, USA) and with a constant flow rate of 600 μL min−1 (0 min ...
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bioRxiv - Cell Biology 2019Quote: ... and then probed with primary antibody (see Supp. Table 2) followed by the appropriate HRP-conjugated secondary antibody (1:5000) (Agilent, Santa Clara, CA).
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bioRxiv - Bioengineering 2021Quote: ... samples were subjected to a treatment with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909; Agilent Technologies, Santa Clara, CA). Immunofluorescence staining was then performed using antibodies to detect CD31 (dilution 1:250 ...