Labshake search
Citations for Agilent :
1001 - 1050 of 1131 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... The antigen retrieval process involved incubating the sections with DAKO Target Antigen Retrieval Solution (diluted 1:10; Agilent Technologies, USA) in a water bath at 90 °C for 45 minutes ...
-
bioRxiv - Genomics 2024Quote: ... each library underwent a reconditioning PCR to reduce heteroduplexes: libraries were concentrated down to 5 µl and mixed with 10 µl of Herculase Buffer (Agilent), 5 µl of 2.5 nM dNTPs ...
-
bioRxiv - Neuroscience 2024Quote: ... blots were washed 3 times during 10 min with PBS-T and incubated with horseradish peroxidase-conjugated secondary antibody (Dako) for 1 h and washed again 3 times ...
-
bioRxiv - Molecular Biology 2024Quote: ... Approximately 20 µL of the concentrated DNA (∼800 ng-1000 ng) was used to transform 100 µL of XL-10 Gold ultra competent cells (Agilent) (genotype ...
-
bioRxiv - Neuroscience 2024Quote: ... Chromogen development was performed using a 10 min incubation followed by 5 min water rinse and a 10 min counterstain with Dako EnVision Flex hemotoxylin (K800821-2, Dako). Slides were transferred to Sakura Prisma Autostainer for dehydration and coverslipping.
-
bioRxiv - Physiology 2024Quote: ... interfaced to a nanoscale reversed-phase high-pressure liquid chromatography (Tempo) with a 10 cm-180 micron ID glass capillary packed with 5-µm C-18 ZorbaxTM beads (Agilent) as described before 37.
-
bioRxiv - Microbiology 2024Quote: ... frozen stocks of both pDC2-cam-coCas9-U6-yDHOODH and pCC1-hDHFR-yFCU wild-type plasmids in XL-10 competent cells (Agilent) were initially expanded by inoculation in 200mL LB broth supplemented with 100ug/mL Ampicillin and grown for 18h at 37°C overnight under shaking conditions at 200rpm ...
-
bioRxiv - Cancer Biology 2024Quote: ... and imaged with the 4x objective every 12 h for 10-14 days using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). Images were processed for data acquisition using BioTek GenV software (Agilent) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Quenching of endogenous peroxidase was performed by 10 min of incubation with Peroxidase-Blocking Solution at RT (S2023, Dako, Agilent). Non-specific unions were blocked using 5 % of goat normal serum (16210064 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Quenching of endogenous peroxidase was performed by 10 min of incubation with Peroxidase-Blocking Solution at RT (S2023, Dako, Agilent). Non-specific unions were blocked using 5 % of goat normal serum (16210064 ...
-
bioRxiv - Cell Biology 2024Quote: ... Frozen 10 µm sections were prepared using a cryostat and immunostained with guinea pig anti-insulin polyclonal antibody (1:100; Dako IR002 ...
-
bioRxiv - Cancer Biology 2024Quote: ... with 0.1% v/v Ammonium Hydroxide (Alfa Aesor) in 90% Water (Honeywell)/10% Acetonitrile (BioSolv) containing 5 µM Infinitylabs Deactivator Solution (Agilent). Solvent B was 90% Acetonitrile / 10% Water containing 5 µM Infinitylabs Deactivator Solution ...
-
bioRxiv - Cancer Biology 2022Quote: ... fixed tissues were embedded in paraffin and 4 mm sections were histologically processed for hematoxylin-eosin staining and immunohistochemistry using anti-human Ki67 antibody (DAKO #M7240, 1:75, 30 min at 37°C). All these procedures were performed using standardized protocols by Atrys Health S.A.
-
bioRxiv - Cancer Biology 2021Quote: ... In vivo psoralen cross-linking of the DNA was achieved by a repetitive exposure of living cells to 4,5’,8-trimethylpsoralen (10 μg/ml final concentration) followed by irradiation pulses with UV 365-nm monochromatic light (UV Stratalinker 1800; Agilent Technologies). The cells were then lysed with cell lysis buffer (buffer C1 ...
-
bioRxiv - Cell Biology 2020Quote: 3000 control or p107KO cells or p107KO cells 24 hours post transfection with pCMV6-OCT-HA-eGFP alone or together with p107fl or p107mls were seeded in DMEM (Wisent) containing 10% FBS and 1% penicillin streptomycin on microplates (Agilent Technologies) and treated according to the required experiment ...
-
bioRxiv - Molecular Biology 2021Quote: ... Forty reaction cycles of 10 s at 95 and 30 s at 58 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
-
bioRxiv - Biochemistry 2021Quote: ... Nbs were mixed with hArc-CTD in 1.3-fold molar excess for a final complex concentration of 10 mg/mL and 50 μl of the mixture injected onto an AdvanceBio SEC 130 column (Agilent Technologies) pre-equilibrated with the running buffer (20 mM HEPES ...
-
bioRxiv - Biochemistry 2020Quote: The peptide was purified by high-performance liquid chromatography (HPLC) on a C4 column (Phenomenex Jupiter C4, 5 μm, 300 Å, 250 × 10 mm, Agilent) using an acetonitrile/water gradient containing 0.1% TFA ...
-
bioRxiv - Microbiology 2021Quote: ... Cell extracts were each resuspended in 50 μl methanol and 10 μl applied to high-performance liquid chromatograph (HPLC) (Agilent Technologies). AzoSM was detected at 365 nm on a C-8 column with a flow rate of 0.5 ml/min in methanol/water 90:10 ratio buffered with 1mM ammonium formate and 0.2% formic acid (Figure S8) ...
-
bioRxiv - Microbiology 2021Quote: ... and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 12 μM medronic acid (InfinityLab Deactivator additive, Agilent Technologies). The flow rate was kept at 250 μl/ml consisting of a 2 minutes hold at 10% B ...
-
bioRxiv - Plant Biology 2021Quote: ... and 0.25 μm film of 95% dimethyl/5% diphenylpolysiloxane) with a precolumn (10 m J&W integrated with Agilent 122-5532G) was used for compound separation ...
-
bioRxiv - Immunology 2021Quote: ... SC-macrophages or SC-macrophages treated with 10 nM RvD1 as above were seeded (30,000 cells/well) on a Seahorse XF96 cell culture plate (Agilent, #102601-100). The cells were incubated in glucose-free ...
-
bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% H2O2 / Methanol for 10 minutes followed by incubation with Dako Dual Endogenous Enzyme Block reagent (Agilent Dako, S2003) for 30 min at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... GC-MS analysis was performed using an Agilent 7890B-5977A system equipped with a 30 m + 10 m × 0.25 mm DB-5MS + DG column (Agilent J&W) connected to an MS operating in electronimpact ionization (EI ...
-
bioRxiv - Biochemistry 2022Quote: ... The samples were loaded on a Superdex 200 Increase 10/300 GL column (Cytiva) run by a 1260 Infinity II HPLC (Agilent Technologies) at 0.6 mL/min ...
-
bioRxiv - Biochemistry 2022Quote: ... 60 µL of purified PKD1KD at 2 mg/mL was injected onto an S200 10/300 column (Cytiva) connected to a 1260 Infinity HPLC (Agilent Technologies). A MiniDawn Treos (Wyatt ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were then centrifuged at 40,000 × g for 10 minutes after which 50μL of each sample was transferred to an amber V-shaped glass chromatography vial (Agilent #5184-3554) containing 80μL N-methyl-trimethylsilyl-trifluoroacetamide (MSTFA ...
-
bioRxiv - Biophysics 2022Quote: ... The samples were loaded on a Superdex 200 10/300 Increase column (Cytiva) at a temperature of 20°C run by a 1260 Infinity II HPLC (Agilent Technologies) at 0.6 ml/min ...
-
bioRxiv - Biochemistry 2022Quote: ... Splitless injection (injection temperature 270 °C) onto a 30 m + 10 m × 0.25 mm DB-5MS + DG column (Agilent J&W) was used ...
-
bioRxiv - Cell Biology 2022Quote: ... Arrays were then washed with probing buffer (0.1% Tween 20, 1% BSA, 10% PBS) and incubated for 1.5 h in a hybridization chamber (Agilent, Santa Clara, CA) in a reaction mixture containing 80 μg of purified His-SETD6 in probing buffer in a total reaction volume of 950 μl ...
-
bioRxiv - Microbiology 2020Quote: ... the amount of dT10-PEG2-dT10-10% Biotin strands per NP was estimated using a Varian Cary 100 Bio UV-visible spectrophotometer (Agilent Technologies) and a quartz cuvette of 1 cm path length.
-
bioRxiv - Developmental Biology 2020Quote: ... RIN scores (RIN = 10 for all 6 samples) were measured on a BioAnalyzer 2100 using the RNA 6000 Nano assay (Agilent Technologies). Poly(A)-purified mRNA-seq library preparation was performed according to the TruSeq Stranded mRNA Reference Guide (Illumina) ...
-
bioRxiv - Neuroscience 2021Quote: Sonication parameters were generated by a Windows 10 computer and sent to a function generator (Agilent 33220A, Agilent Technologies, Santa Clara). The output of the function generator was passed through an amplifier (E&I ...
-
The effect of exercise on the protein profile of rat knee joint intra- and extra-articular ligamentsbioRxiv - Molecular Biology 2020Quote: ... 30μg of the soluble fraction was subjected to an in-solution digestion on 10 μl Strataclean™ resin (Agilent Genomics, UK) followed by reduction and alkylation in 3 mM dithiothreitol (DTT ...
-
bioRxiv - Cancer Biology 2020Quote: ... Splitless injection (injection temperature 270°C) onto a 30 m + 10 m × 0.25 mm DB-5MS+DG column (Agilent J&W) was used ...
-
bioRxiv - Cancer Biology 2020Quote: ... Splitless injection (injection temperature 270°C) onto a 30 m + 10 m × 0.25 mm DB-5MS+DG column (Agilent J&W) was used ...
-
bioRxiv - Physiology 2021Quote: ... fully differentiated myotubes were treated for 24h with 10 ng/ml rNRTN before being subjected to extracellular flux analysis (XF24, Seahorse Bioscience). The mitochondrial stress test assay was performed with 1 mM pyruvate and 5 mM glucose ...
-
bioRxiv - Genomics 2021Quote: ... cDNA were amplified by 10 PCR cycles and purified before assessing their quality on the bioanalyzer (2100 high sensitivity DNA assay, Agilent Technologies). 150 pg of cDNA were used as input to prepare the libraries using the Nextera XT DNA kit (Illumina) ...
-
bioRxiv - Cell Biology 2021Quote: ... Splitless injection (injection temperature 270 °C) onto a 30 m + 10 m × 0.25 mm DB-5MS + DG column (Agilent J&W) was used ...
-
bioRxiv - Cell Biology 2021Quote: ... washed three times in PBST containing 10 µg/ml DAPI for 5 minutes and mounted with a coverslip in fluorescent mounting media (Dako, S3023). All images were taken using a Zeiss LSM 710 confocal microscope and quantified manually using ImageJ software ...
-
bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed on 1:10 diluted cDNA using either SYBR-containing Brilliant III Ultra-Fast QPCR Master Mix (Agilent Technologies) and gene-specific primers ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 20,000 × g for 10 minutes after which 50μL of each sample was transferred to an amber V-shaped glass chromatography vial (Agilent #5184-3554) containing 80μL N-methyl-trimethylsilyl-trifluoroacetamide (MSTFA ...
-
bioRxiv - Biochemistry 2023Quote: ... Each sample was injected into the size-exclusion chromatography column (Superdex 75 10/300, Cytiva) linked to an HPLC system (Agilent 1200) and was subsequently measured in-line on the X-ray cell ...
-
bioRxiv - Biophysics 2023Quote: ... Sample was loaded onto a Superdex 200 Increase 10/300 GL column (Cytiva) run by 1260 Infinity II HPLC (Agilent Technologies) at 0.6 ml/min ...
-
bioRxiv - Cancer Biology 2024Quote: ... 5µm sections were deparaffinized with xylene and rehydrated through graded ethanol washes followed by antigen retrieval in sodium citrate buffer (10 mM, pH 6.0) with a pressure cooker (Dako, Carpinteria, CA), blocked ...
-
bioRxiv - Microbiology 2024Quote: ... 40x 10 sec 95°C and 30 sec 60°C with the Brilliant III Ultra-Fast qRT-PCR master mix (Agilent, 600884) were used according to manufacturer’s protocol.
-
bioRxiv - Cell Biology 2024Quote: ... Blocking was done in Primary Blocking Buffer (10% donkey serum, 0.1% saponin, 0.05 mg/mL sonicated salmon sperm DNA (Stratagene, La Jolla, California) in PBS for 1 hour at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 with Dako Target Retrieval Solution (Agilent, USA), in a Lab Vision PT Module (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2024Quote: ... A 500-μL aliquot of the digestate was mixed with an equal volume of internal standard (10 μg/L Iridium) and analyzed using ICPMS (Agilent 7700) with an external calibration curve.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slides were then treated with 3% hydrogen peroxide for 10 min and then blocked with Serum-Free Protein Block (Dako, X0909) for 30 min ...