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Citations for Agilent :
51 - 100 of 500 citations for Rat CPS1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Primary rat neurons or human i3Neurons were plated on Seahorse XFp plates (Agilent) at a density of 40,000 cells/well ...
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bioRxiv - Molecular Biology 2023Quote: ... serotype 6 (AAV6) vector plasmids were cloned into the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA), comprised of inverted terminal repeats (ITRs ...
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bioRxiv - Genetics 2024Quote: ... serotype 6 (AAV6) vector plasmids were cloned into the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA), comprised of inverted terminal repeats (ITRs ...
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bioRxiv - Molecular Biology 2020Quote: ... The pBluescript plasmid was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Bioengineering 2022Quote: ... Inc.) into the pAAV-MCS plasmid (Agilent Technologies containing inverted terminal repeats (ITRs) ...
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bioRxiv - Cell Biology 2023Quote: ... coli strain harbouring a pRARE2 plasmid (Agilent) using Terrific Broth cultured in a LEX Bioreactor (Epiphyte3) ...
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bioRxiv - Neuroscience 2021Quote: ... shuttle plasmids were co-transfected with the pDF9 helper plasmid into HEK293-AAV cells (Agilent Technologies, Santa Clara, USA). Cells were lysed 72h following transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were then stained with polyclonal rabbit anti-rat IgG (1:200, Dako), or EnVision rabbit (Dako ...
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bioRxiv - Microbiology 2021Quote: ... A rat anti-FLAG monoclonal antibody (200474) was purchased from Agilent (Santa Clara, CA). MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I ...
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bioRxiv - Neuroscience 2023Quote: ... Primary α-DAT was detected by using secondary rabbit anti-rat (1;1,000; Dako, P0450). Upon visualization by using the ECL Advanced Chemiluminescence kit (GE Healthcare Life Sciences® ...
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bioRxiv - Cancer Biology 2023Quote: ... Ki-67 was detected using a rat an,-Ki67 monoclonal an,body (Dako M7249) at a 1:25 dilu,on followed by bio,nylated goat an,-rat an,body secondary (Jackson ImmunoResearch 112-065-167 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant viruses were produced in 293T cells by cotransfecting pLPCX plasmids with pVPack-GP and pVPack-VSV-G packaging plasmids (Stratagene). The pVPack-VSV-G plasmid encodes the vesicular stomatitis virus (VSV ...
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bioRxiv - Evolutionary Biology 2022Quote: We isolated the plasmids containing the three segregating lacZ promoter variants from the MG1655 strain using StrataPrep Plasmid Miniprep Kit (Agilent). We also isolated the promoter- less vector pMV001 the same way ...
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bioRxiv - Neuroscience 2023Quote: AAV was produced by HHMI-Janelia Viral Tools using a PEI triple transfection protocol into AAV293T cells (an ITR-containing plasmid, 2/9 capsid helper from UPenn Vector Core, and the E1-deleted pHelper plasmid from Agilent). The cells were grown under serum-free conditions (three 150mm culture dishes at ∼3×107 cells/dish for each 100 µl batch) ...
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bioRxiv - Neuroscience 2021Quote: ... pUCmini-iCAP-PHPeB and pHelper plasmid (Agilent, U.S.A.) were transfected into cells at 80%–90% confluency using polyethyleneimine (Polysciences ...
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bioRxiv - Molecular Biology 2019Quote: ... the plasmid was amplified using PfuTurbo (Agilent 600252). The product was DpnI-treated ...
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bioRxiv - Synthetic Biology 2020Quote: ... plasmids were extracted using a maxiprep kit (Agilent), their concentration was measured ...
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bioRxiv - Genetics 2021Quote: ... in the pBluescript II SK plasmid (Agilent Technologies). The final targeting vector thus contained 4.8 kbp upstream and 7.2 kbp downstream of Col3a1 exon 6.
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bioRxiv - Biophysics 2020Quote: ... DNA fragments: one from the pBlueScriptIISK+ plasmid (Stratagene) and the other from the pNLrep plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... into the pBluescript II KS-plasmid (Agilent Technologies). For in-frame fusion with Cdk2 and Tti1 fragments ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids were mutated using the QuickChange technique (Agilent). Constructs were co-transformed into BL21(DE3 ...
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bioRxiv - Neuroscience 2020Quote: ... with the 5×SRE-Luc reporter plasmid (Stratagene) and EF1αLacZ (β-galactosidase [β-Gal]) ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant plasmids were transformed in XL1Blue (Agilent Technologies) or Top10 (Thermofisher scientific ...
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bioRxiv - Bioengineering 2023Quote: ... the packaging plasmid (see above) and pHelper (Agilent) for the three adenoviral helper genes ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used for paraffin immunostaining were: rat anti-mouse Ki67 monoclonal antibody (MIB-5, Dako) and rabbit anti-mouse Yap1 antibody (D8H1X ...
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bioRxiv - Microbiology 2019Quote: ... A DNA fragment containing the NP868R ORF was recovered from the plasmid by digestion with NotI and PvuI and ligated into the plasmid pCMV-Script (Agilent Technologies), cut with the same two restriction enzymes ...
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bioRxiv - Plant Biology 2019Quote: ... The exact point mutations for each of the three Hsf1 missense mutations were engineered into the cDNA of ZmHK1 in the plasmid P415-CYC1-ZmHK1 plasmid with the QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) using the manufacturer’s specifications.
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bioRxiv - Neuroscience 2020Quote: ... the original plasmid was digested with DpnI restriction enzyme and the remaining mutagenized plasmid was transformed into XL10-Gold® ultracompetent cells (Agilent). Integrity of the insert sequence and introduction of the point mutation were confirmed by Sanger sequencing of the isolated plasmid DNA.
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bioRxiv - Genetics 2019Quote: The Ycp50-rad50-L1240F plasmid was randomly mutagenized by propagating the plasmid in XL1 red bacterial mutator (mutS, mutD, mutT) strain (Agilent Technologies). rad50Δ mec1Δ sml1Δ sae2Δ spore (JPY1953 ...
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bioRxiv - Neuroscience 2020Quote: ... Transfer plasmid was constructed by inserting cDNA fragment and WPRE sequence into an AAV backbone plasmid (pAAV-CMV, Stratagene, San Diego, USA). For production of an AAV-DJ vector (AAV-DJ-rSyn-GCaMP6s) ...
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bioRxiv - Neuroscience 2023Quote: ... The transfer plasmid was constructed by replacing the CMV promoter of an AAV backbone plasmid (pAAV-CMV, Stratagene, San Diego, CA, USA) with a human Syn promoter ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were maintained in E.coli XL-1 Blue (Stratagene). Bacmids were maintained in DH10Bac (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Genetics 2020Quote: ... a modified pBlueScript-KS+ plasmid (Stratagene, La Jolla, CA) in which two AscI sites ...
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bioRxiv - Developmental Biology 2022Quote: ... and cloned into the pBluescript II SK plasmid (Stratagene). Digoxigenin-labeled RNA probe was prepared according to the manufacturer’s instruction (Roche).
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bioRxiv - Neuroscience 2019Quote: ... followed by subcloning into plasmid pCMV2b (Agilent Technologies, 211172) for expression of FLAG-tagged fusion proteins ...
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bioRxiv - Microbiology 2020Quote: The plasmid pBlueScriptII (Agilent Technologies Inc., Santa Clara, CA) was digested with Hind III restriction enzyme digestion ...
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bioRxiv - Developmental Biology 2020Quote: ... and transformation of plasmids into the Ultracompetent cells (Agilent). The plasmid was sequenced to verify the point mutation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Biochemistry 2024Quote: ... Mutated plasmids were cloned using pfuTurbo DNA polymerase (Agilent) and parent plasmids were degraded by DpnI (NEB) ...
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bioRxiv - Systems Biology 2020Quote: ... mouse and naked mole rat cells were plated onto a Seahorse XF96 Cell Culture Microplate (Agilent) at a cell density of 105,000 and 80,150 cells per well respectively to allow the cells to be 100% confluent ...
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bioRxiv - Genomics 2019Quote: ... and hybridized onto SurePrint G3 Rat GE 8 x 60K Microarrays v2 (AMADID 074036; Agilent Technologies) for 16-20 h at 65°C in an Agilent oven with rotisserie ...
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bioRxiv - Developmental Biology 2019Quote: ... The following antibodies were incubated overnight at 4°C: rat anti-human CD31 (DAKO, M082329-2), rabbit anti-human S1PR1 (Santa Cruz ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µg of the plasmid carrying the relevant recombinant provirus was co-transfected with 2 µg of plasmid pMD2.G (a kind gift from D. Trono) and 2 µg of plasmid PVPackgagpol (#217566 from Stratagene, La Jolla, CA) or plasmid pCMV-1′R8.74 (a gift from D ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... mouse (Fig. S8b) and naked mole-rat (Fig. 2a-c) marrowSeahorse XF96 Cell Culture Microplates (Agilent Technologies) between 50-250*103 cells per 200µl of the following culture media ...
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bioRxiv - Immunology 2023Quote: ... using 1 µg/mL of rat anti-mouse IgM (AbD Biotec) or rabbit anti-mouse IgG1 (Dako) capture antibody ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transformed into chemically competent XL-10 Gold (Agilent), and colonies were grown on LB agar plates at 37 °C for 16-20 h and at room temperature for an additional 20-24 h ...
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bioRxiv - Plant Biology 2019Quote: ... Plasmids were transformed into BL21-DE3-RIPL competent cells (Agilent) and induced with 0.5 mM IPTG for 4 hours ...
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bioRxiv - Plant Biology 2021Quote: ... Expression plasmids were transformed into XL10-Gold Ultracompetent Cells (Agilent). DNA was extracted using ZR Plasmid Miniprep - Classic (Zymo Research ...
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bioRxiv - Bioengineering 2022Quote: ... pAAV2/9 and a helper plasmid (Stratagene, La Jolla, CA) as previously described92 ...