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Citations for Agilent :
51 - 100 of 2720 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with 100 µL of XF Base Media (Seahorse Bioscience) containing 2 mM L-Glutamine ...
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bioRxiv - Biophysics 2021Quote: hnRNPA1* (where * denotes that the hexa-peptide 259-264 is deleted) protein and deletion constructs were expressed as N-terminally tagged hSUMO fusion proteins in BL21 (DE3) RIPL cells (Agilent) in LB media ...
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bioRxiv - Biophysics 2023Quote: ... The monodisperse sample solutions of proteins were injected onto a size exclusion column (David and Perez 2009) (SEC-3, 150 Å; Agilent) using an Agilent HPLC system and eluted into the capillary cell at a flow rate of 0.3 ml min-1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slides were then treated with 3% hydrogen peroxide for 10 min and then blocked with Serum-Free Protein Block (Dako, X0909) for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cytospin preparations of BALF cells were blocked with Dako protein block (Agilent) for 10 min ...
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bioRxiv - Biophysics 2023Quote: ... Bacterial protein expression was from BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies), unless otherwise stated ...
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bioRxiv - Biochemistry 2023Quote: Protein complexes were overproduced in BL21-CodonPlus (DE3) Escherichia coli cells (Agilent) grown in high salt LB Broth (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were blocked with serum-free protein block solution (Dako, X0909). A permeabilization step was performed using 0.1% Triton X-100 in water for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: Primary brown adipose cells were isolated and cultured for 3 days before plated in XF cell culture microplates (Seahorse Bioscience). Cells (10,000 cells ...
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bioRxiv - Bioengineering 2024Quote: ... and cells were imaged every 3 hours for 48 hours using a Cytation 5 cell imaging multimode reader (Agilent Technologies) .
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with 3% hydrogen peroxide to quench endogenous peroxidase activity and 15 minutes in serum-free protein block (DAKO, Glostrup, Denmark). Sections were then subjected to appropriate antigen retrieval methods (if needed ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated for 3 h before being analyzed with NovoCyte Flow Cytometer (Agilent). For the other markers ...
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bioRxiv - Bioengineering 2024Quote: ... Bevacizumab-sensitive/resistant U87 cells were cultured identically to wild-type U87 cells.79 Cells were screened for mycoplasma every 3 – 4 months with the MycoSensor qPCR Assay Kit (Agilent Technologies).
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... Chromagen was developed using the DAB (3, 3-diaminobenzidine) reagent (Dako). Sections were counter-stained using pre-filtered Mayer’s haematoxylin (Amber Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Antigen–antibody complexes were revealed with 3-3’-diaminobenzidine (K3468, Dako). Sections were counterstained with haematoxylin (Dako ...
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bioRxiv - Cell Biology 2020Quote: ... Antigen-antibody complexes were revealed with 3-3’-diaminobenzidine (K3468, Dako). Sections were counterstained with hematoxylin (Dako ...
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bioRxiv - Physiology 2022Quote: ... Antigen–antibody complexes were revealed with 3-3′- diaminobenzidine (K346811, Dako) or the DAB (Polymer ...
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bioRxiv - Immunology 2021Quote: ... and Protein block (Protein block (Dako) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... cells were pre-incubated with blocking buffer (Protein Block Serum Free solution, DAKO) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... organoids were seeded in 3 μL matrigel per well in XF24 cell culture microplates (Seahorse Bioscience). 1 hour before the measurements ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were analyzed 3 days after transfection using flow cytometry (Dako CyAN ADP, or ACEA Quanteon), as described30,64 ...
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bioRxiv - Microbiology 2021Quote: ... Tissue sections were colored via DAB-staining (3, 3-diaminobenzidine; Dako, K3468).
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bioRxiv - Cancer Biology 2022Quote: ... 3’-Diaminobenzidine (DAB, Agilent) and then counterstained with haematoxylin (Abcam) ...
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bioRxiv - Microbiology 2021Quote: The CPB mutant virus was generated using a two part QuikChange site-directed mutagenesis (Agilent, Santa Clara, CA) of the TE12 SINV cDNA clone ...
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bioRxiv - Immunology 2022Quote: ... values at the endpoint (36 hours after virus inoculation) were determined using the RTCA software version 2.1.0 (Agilent). Percent neutralization was calculated as the CI in the presence of mAb divided by the cells only (no-CPE control ...
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bioRxiv - Cell Biology 2022Quote: ... All three proteins were expressed in BL21-Gold (DE3) competent cells (Agilent Technologies; 230132). The cells were cultured in 4 L of Luria Bertani broth (LB ...
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bioRxiv - Biophysics 2023Quote: ... cerevisiae Cdc6 protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with pGEX-6P-1 wt GST-cdc6 using 400 µM IPTG for 16 h at 16 °C ...
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bioRxiv - Biophysics 2023Quote: dCas9-Cys protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with plasmid 10xHis-MBP-TEV-S ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified from BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent, 230280) as follows ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... cells were washed 3 times with warm PBS and fixed on coverslips with mounting reagent (DAKO,S3025). Cell images were taken by confocal microscopy (Fluoview FV100 ...
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bioRxiv - Microbiology 2022Quote: ... Optical density was read at 405 nm on a Cytation 3 Cell Imaging Multi-Mode Reader (Agilent) and analysed in Bioek Gen5 (Agilent).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... in 3 cycles for 3 hours using the Seahorse XFe24 analyzer system (Agilent Technologies). Inhibitors and substrates were used at the following concentrations ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound antibody was removed with washing buffer and the fraction of cells with surface protein labeled with CD44 antibody was determined using a MoFlo cell sorter (Dako Cytomation).
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bioRxiv - Genomics 2019Quote: ... Recombinant PA-Tnp protein was expressed in BL21-Gold(DE3) competent cells (Agilent cat # 230132) and purified using nickel beads ...
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bioRxiv - Plant Biology 2023Quote: ... Substrate proteins were induced and expressed in Escherichia coli BL21 CodonPlus(DE3)-RIL cells (Stratagene) over night at 18°C after induction with 1 mM IPTG ...
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bioRxiv - Biophysics 2023Quote: Halo-tagged dCas9 protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with pET302-6His-dCas9-halo (Addgene #72269 ...
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bioRxiv - Neuroscience 2023Quote: ... All other proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ overnight ...
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bioRxiv - Cell Biology 2023Quote: Bsp1 proteins were expressed overnight at 20°C in BL21 pLysS Gold competent cells (Agilent) using autoinduction LB medium (Formedium) ...
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bioRxiv - Developmental Biology 2019Quote: ... service pack 3 (Agilent Technologies).
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bioRxiv - Microbiology 2023Quote: ... 3 × 150 mm column (Agilent), column temperature 50 °C ...
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bioRxiv - Microbiology 2022Quote: ... The optical density (OD) was measured at 405nm using Cytation™ 3 Cell Imaging Multi-Mode Reader (Agilent) and data was analysed using BioTek Gen5 software (Agilent) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein blocking was performed using protein block solution (DAKO) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... UV-inactivation was carried by irradiation of virus-containing supernatant in a 15cm plate prior the concentration step in Stratalinker 1800 (Stratagene) at the standard power for 3 times 3min with swirling of the plate between each round ...
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bioRxiv - Neuroscience 2023Quote: ... woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) and SV40 polyadenylation signal was assembled using a modified helper-free system (Stratagene) as a serotype 2/1 (rep/cap genes ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were incubated for 1 hour at room temperature with serum-free protein block (Dako X0909) to block non-specific binding sites followed by Cleaved-Casp3 primary antibody (Abcam ...
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bioRxiv - Biochemistry 2021Quote: HMECs were cultured at a density of 3 x 104 cells/well in 24-well Seahorse XFe24 plates (Agilent) and incubated at 37 °C under a humidified 5% CO2 atmosphere overnight in the presence or absence of DMF ...