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Citations for Agilent :
901 - 950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... these pools were all checked for concentration with the Qubit and fragment size distribution was measured again using a HS D1000 ScreenTape run on a Tapestation 2200 (Agilent Technologies, Santa Clara, CA, USA). The capture libraries were pooled in equimolar quantities to give a single sample at 10 nM DNA with an average fragment size of 400 bp ...
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bioRxiv - Molecular Biology 2024Quote: ... and used a HS D1000 ScreenTape run on a Tapestation 2200 (Agilent Technologies, Santa Clara, CA, USA) to check the size distribution ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA Integrity Number (RIN) for all samples was 10 as assessed by an RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2024Quote: ... Separations were performed on a HILIC-Z column (150 × 2.1 mm, 2.7 µm; Agilent Technologies). The mobile phase was composed of 20 mM ammonium formate + 0.25% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... and AffinityScript reverse transcriptase (Agilent). cDNA products were diluted in water (1:5 ...
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bioRxiv - Molecular Biology 2024Quote: ... LC-MS analysis was performed using an Agilent 1290 Infinity LC (Agilent Technologies, Santa Clara, CA) equipped with a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... and TapeStation High Sensitivity RNA ScreenTape Analysis (Agilent, 5067-5579). Libraries were pooled in equal quantity and sequenced using Illumina HiSeq 2500 (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... Digested peptides were separated using a 50 cm reversed-phase column packed in-house (Agilent Poroshell EC-C18, 2.7 µm, 50cm x 75 µm) and eluted from the column at a flow rate of 300 nL/min ...
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bioRxiv - Molecular Biology 2024Quote: ... The digestion was quenched with 10% formic acid (FA) and the resulting peptides were cleaned-up in an automated fashion using the AssayMap Bravo platform (Agilent Technologies) with a corresponding AssayMap C18 reverse-phase column ...
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bioRxiv - Developmental Biology 2024Quote: ... Post cDNA amplification and post-library construction quality control was performed using the Agilent Bioanalyzer High Sensitivity kit (Agilent 5067–4626). Libraries were sequenced using a NovaSeq 6000 and the S2 flow cell ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Cholesterol and its deuterated standard were separated on an Eclipse Plus C8 column (Agilent), and peaks were integrated with TraceFinder 5.1 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2100 BioAnalyzer D1000 kit (Agilent 50671504) and Illumina MiSeq Nano v2 QC.
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bioRxiv - Molecular Biology 2024Quote: ... and separated on a 50-cm reversed phase column packed in-house (Poroshell EC-C18, 2.7 μm, 50 cm × 75 μm; Agilent Technologies). Proteome samples were eluted over a linear gradient of a dual-buffer setup with buffer A (0.1% FA ...
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bioRxiv - Molecular Biology 2024Quote: ... Data was analyzed using the MxPro software (Agilent). The relative occupancy of each factor was determined by comparison with a standard curve of amplification efficiency for each amplicon using a range of input DNA amounts generated in parallel with each qPCR run.
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bioRxiv - Molecular Biology 2024Quote: ... 30 s at 58 °C and 30 s at 72 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA integrity was confirmed using a 2100 Bioanalyzer (Agilent Technologies). RNA libraries were prepared using the Illumina® Stranded Total RNA Prep ...
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bioRxiv - Molecular Biology 2024Quote: ... and checked for concentration and quality on DNA chips with a Bioanalyzer (Agilent). 75-bp single read sequences were generated on the NextSeq 500 sequencer according to manufacturer’s instructions (Illumina).
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bioRxiv - Cancer Biology 2024Quote: ... cDNA library quality and quantity were determined using an Agilent 2100 bioanalyzer using the High Sensitivity DNA Reagents kit (Agilent, 5607-4626) and then sequenced on an Illumina NovaSeq 6000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and developed using DAB as chromogen (Agilent Dako, Santa Clara, CA, USA). Primary antibodies used in the study included EGFR (D1P9C ...
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bioRxiv - Molecular Biology 2024Quote: ... Integrity and quality of RNA was determined on a Bioanalyzer (Agilent, #5067-1511). Poly(A ...
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bioRxiv - Molecular Biology 2024Quote: ... using the QuikChange site-directed mutagenesis kit (Agilent, Waldbronn, Germany). The KpnI / XhoI fragment of pcDNA3.1-TRIP6-iuACG-HA was cloned into pcDNA3.1 to obtain pcDNA3.1-TRIP6ΔAUG1-iuACG-HA ...
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bioRxiv - Molecular Biology 2024Quote: ... All site-directed mutagenesis (SDM) reactions were carried out using the Quick-Change Lightning SDM Kit (Agilent, USA), the primers listed in Supplementary Table S1 ...
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bioRxiv - Molecular Biology 2024Quote: Mito Stress Assay: Mitochondrial oxygen consumption rate (OCR) was measured using a Seahorse XFe96 analyzer (Seahorse Bioscience, North Billerica, MA, USA). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... The quality and concentration of libraries were assessed with Bioanalyzer (Agilent, #5067-4626) and Qubit (Thermofisher ...
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bioRxiv - Cancer Biology 2024Quote: ... L2086F mutations were made using site-directed mutagenesis following the manufacturer’s protocol (Agilent) using primers listed in Supplementary Table S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized with AffinityScript One-Step RT-PCR Kit (Agilent, 600188). Each cDNA sample was amplified in triplicate using the SYBR Green and Platinum Taq polymerase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... quenched samples were passed through a co-immobilized pepsin/fungal XIII protease column (1mm × 2cm) at 50 µl min−1 and the digested peptides were captured on a 1mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell counting was conducted using an Agilent BioTek Cytation 5 (Agilent Technologies) imaging system with Gen5 software (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... imaging system with Gen5 software (Agilent Technologies) following the manufacturer’s instructions [12].
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s instructions (Agilent Technologies). Sequencing analyses were performed to check the integrity of all plasmid constructs (full length SLC40A1 cDNA sequenced after each site-directed mutagenesis).
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions employing SYBRgreen fluorescent reagent and/or EvaGreen™ were performed in the Stratagene Mx3000P system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: RNA was isolated from cells using the Absolutely RNA miniprep kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The quality and quantity of RNA were confirmed using NanoDrop and Bioanalyzer RNA 6,000 nano Kit (Agilent). Samples with RNA integrity number values exceeding 6.5 were used for RNA sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... in XF DMEM medium (Agilent #103575-100)] in atmospheric air at 37°C ...
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bioRxiv - Neuroscience 2024Quote: OCR assay: Cells were washed and incubated for 1 hour with 180 μL assay medium [in mmol/L: 5 glucose (Agilent #103577-100), 1 pyruvate (Agilent #103578-100) ...
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bioRxiv - Neuroscience 2024Quote: ... 1 pyruvate (Agilent #103578-100), 2 glutamine (Agilent #103579-100 ...
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bioRxiv - Neuroscience 2024Quote: ... 6.5-9) and concentration (5-50 ng/µl; 260/280 values >1.7) were evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, CA) and Nanodrop (Thermo-fisher ...
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bioRxiv - Neuroscience 2024Quote: ... The EGFP and mCherry dynamin-2 mutations A618T and S619L were constructed by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA). Neuropeptide Y fused to mCherry (NPY-mCherry ...
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bioRxiv - Microbiology 2024Quote: ... Site-directed mutagenesis to generate shfP mutants was achieved using the QuikChange kit (Agilent). All plasmids were integrated into the B ...
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bioRxiv - Microbiology 2024Quote: ... 10% HFBA [Heptafluorobutyric acid] was added to 0.2% final and each individual sample was cleaned up using 100 µL Bond Elut OMIX C18 pipette tips (Agilent) according to manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: ... by standard protocol using Genomic DNA ScreenTape and TapeStation Software (Agilent, Santa Clara, CA, United States) (kindly provided by prof ...
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bioRxiv - Microbiology 2024Quote: ... and their size and molarity were verified using a Tape Station 2200 (Agilent, Waldbronn, Germany). The equimolarly pooled libraries were subsequently subjected to a 100-cycle run on a NovaSeq 6000 apparatus (Ilumina ...
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bioRxiv - Microbiology 2024Quote: ... DNA quality control was performed using 4200 TapeStation System (Agilent, Santa Clara, CA, United States) by standard protocol using Genomic DNA ScreenTape and TapeStation Software (Agilent ...
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bioRxiv - Microbiology 2024Quote: ... RNA quality was determined using 2100 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies).
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bioRxiv - Microbiology 2024Quote: ... The HindIII site in the TRP1 gene of pNZM1090 was removed by QuikChange (Agilent) per manufacturer’s instructions using primers pNZM1090F and pNZM1090R (Table EV6) ...
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bioRxiv - Neuroscience 2024Quote: ... were digested with DpnI and directly transformed into XL10-Gold ultracompetent cells (200315; Agilent). For PIEZO2 mutants ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was produced by AffinityScript (Agilent) in the presence of [α-32P] ATP (10mCi/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... was equipped with a ZORBAX RRHD SB-C18 column (2.1 × 150 mm, 1.8 μm, Agilent) coupled with a 6495B Triple Quadrupole Mass Spectrometer (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: ... Expression levels were analyzed by the delta-delta Ct method with the AriaMX qPCR software (Agilent Aria 1.7). The following primers were used ...
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bioRxiv - Molecular Biology 2024Quote: ... 2µL of the ligation reaction was transformed into BME pre-treated XL10-Gold cells (Agilent) per the manufacturer’s instructions and plated on LB + 100µg/mL carbenicillin plates for selection ...