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Citations for Agilent :
801 - 850 of 1446 citations for Recombinant Human 5' Nucleotidase Ecto CD73 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... RNAs from 4 different experiments (biological quadruplicate) were extracted as described above and analyzed on SurePrint G3 Human Gene Expression 8×60K v2 Microarrays (#G4851B, Agilent Technologies) as previously described (36) ...
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bioRxiv - Neuroscience 2020Quote: ... Human α7-nAChR R208I-GFP and E211N-GFP were generated from pcDNA3.1-CHRNA7-mGFP and human α3-nAChR I284R-GFP and N287E-GFP were generated from human α3-nAChR-GFP by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Serum samples were depleted of the top 14 interfering high-abundant proteins using the Multiple Affinity Removal Column Human 14 (Agilent, USA). Then ...
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bioRxiv - Immunology 2021Quote: ... Exome sequencing was performed on genomic DNA from Patients 1 and 2 and their parents using a SureSelect Human All Exon kit (Agilent Technologies) for targeted enrichment ...
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bioRxiv - Neuroscience 2021Quote: The optimal concentration was determined for each antibody: CD3 (polyclonal rabbit – anti-human CD3 IgG, cat. no. A045201-2, Agilent Technologies), 1:60 ...
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bioRxiv - Biophysics 2021Quote: Mutations were introduced into the human CFTR(E1371S)/pGEMHE and CFTR(D1370N)/pGEMHE sequences using the QuikChange II XL Kit (Agilent Technologies) and confirmed by automated sequencing (LGC Genomics GmbH) ...
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bioRxiv - Cell Biology 2020Quote: ... All hESC lines were analysed for their genetic content by array-based comparative genomic hybridization (Human Genome CGH Microarray 4×44K, Agilent Technologies) as previously described (Jacobs et al. ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated for 45 minutes in 1% dried skim milk in PBS-T with rabbit anti-human C1q (1:300, Dako, A0136) and HRP-conjugated goat anti-rabbit IgG (1:10.000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The library was then enriched by liquid phase hybridization using Agilent SureSelect XT Human All Exon v6 (Agilent Technologies, CA, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... hepaticus versus the tdTomato fluorescent protein (72 h transduction) was quantified in epithelial intestinal HT-29 cells using the Human GE 4x44K v2 Microarray Kit (Agilent Technologies) as we previously detailed [13 ...
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bioRxiv - Microbiology 2022Quote: ... Slides were then washed in PBS/0.05% Tween 20 and incubated with rabbit anti human VWF (A008229-2, DAKO/Agilent tech) 1/500 or rabbit IgG isotype control (AB-105-C ...
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bioRxiv - Microbiology 2023Quote: ... all tissue section slides were treated with a mouse monoclonal Anti-Human IgG p27KIP1 (Clone SX53G8.5 dilution 1:100; Code M7203; Dako Cytomation, Denmark) that cross-reacts with p27kip1 using a protocol described previously [24] ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-CD61 immunohistochemistry (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:75, Clone Y2/51, Dako Omnis, Code Number: M0753) was also employed to evaluate the presence of platelet-enriched microthrombi ...
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bioRxiv - Cancer Biology 2022Quote: Genome-wide copy number variations were assessed using the SurePrint G3 Human aCGH 4×180K Microarray (Agilent Technologies, Santa Clara, CA), according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was fragmented into 180–280 bp by sonication and subjected to library preparation using the Agilent SureSelect Human All Exon V6 Kit (#5190-8864, Agilent Technologies). The enriched libraries underwent paired-end 150bp sequencing on the Illumina HiSeq 2000 platform.
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of the library was hybridized to the Agilent SureSelect XT Human All Exon V4 probes (Agilent Technologies 5190-4634). The targeted exon fragments were captured by Streptavidin beads (Fisher 65602) ...
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bioRxiv - Biophysics 2024Quote: The recombinant human Cα subunit of cAMP-dependent protein kinase with the Phe to Ala mutation in position 100 (PKA-CF100A) was generated from the human PKA-Cα wild-type using Quik-Change Lightning mutagenesis kit (Agilent genomics). The key resource table lists the PCR primers used to modify the pET-28a expression vector encoding for the wild-type human PKA-Cα gene (PRKACA – uniprot P17612 ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using a low-input QuickAmp labeling kit and human SurePrint G3 8×60K microarrays (Agilent Technologies), as previously described (Sulpice et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Flag-tagged wt and Ex19Del human EGFR constructs in the lentiviral VIRSP vector were generated by PCR with Herculase II Fusion DNA Polymerase (Agilent technologies) from EGFR WT (a gift from Matthew Meyerson ...
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bioRxiv - Cell Biology 2023Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8□×□60 K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Pathology 2023Quote: ... with the mouse Prnp encoding the 3F4 epitope (109M, 112M human numbering) was used as a template for site-directed mutagenesis (QuikChange Site Directed Mutagenesis kit™) (Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... Samples from the primary human monolayer experiment were fixed and immunostained for insulin with guinea pig anti-insulin primary antibody (Dako A0564) and Alexa-Fluor 568 goat anti-guinea pig secondary antibody (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Deletions of SP1 sites on human MGMT promoter were performed using Quick-Change XL Site-Directed Mutagenesis kit (Agilent Technologies, USA).34 The mutated promoters were further cloned into pGL3 basic vector ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Biochemistry 2023Quote: ... An alanine mutant library of the SNAP-tagged human truncated mGlu5 receptor (mGlu5-Δ856, designated as WT in the following experiments) was generated using Quick-change strategy (Agilent technologies) and verified by sequencing (Eurofins Genomics)11 ...
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bioRxiv - Cell Biology 2024Quote: Cy3-labeled cRNAs were further hybridized onto Agilent Whole Human Genome kit 4 x 44K slides (Catalog number G4112F, Agilent Technologies) containing 44,397 oligonucleotide probes in 2X Hi-RPM Hybridization buffer (Catalog number 5188-5242 ...
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bioRxiv - Neuroscience 2024Quote: ... Then membranes were probed with the following antibodies: anti-human tau polyclonal antiserum KJ9A (1:5,000; A0024, Dako Cooperation, Carpinteria, CA) and rabbit anti-GAPDH antiserum (1:5,000 ...
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bioRxiv - Neuroscience 2024Quote: The mutant PDI-Ala256 protein was prepared from the full-length wild-type human PDI cDNA using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). For protein purification ...
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bioRxiv - Cell Biology 2024Quote: ... Mutant human PINK1 constructs were generated by QuickChange XL site-directed mutagenesis according to the manufacturer’s guidelines (Agilent Technologies #HPA 200516). The following constructs were made ...
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bioRxiv - Synthetic Biology 2021Quote: ... Separations were performed using a Bio SEC-5 1000 Å 7.8 × 300 mm column (Agilent) with a Bio SEC-5 2000 Å 7.8 × 300 mm guard column (Agilent) ...
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bioRxiv - Immunology 2021Quote: ... followed by 5 μg/ml anti-MPO antibodies (A0398 polyclonal, Agilent Technologies, Santa Clara, CA) or 40 μg/ml isolated serum IgG ...
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bioRxiv - Molecular Biology 2019Quote: ... The column used was Eclipse XDB-C18 column (4.6 x 150 mm 5 μm, Agilent) and eluted with a linear gradient of 0-100% MeCN/H2O with constant 10 mM TEAA additive ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used for paraffin immunostaining were: rat anti-mouse Ki67 monoclonal antibody (MIB-5, Dako) and rabbit anti-mouse Yap1 antibody (D8H1X ...
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bioRxiv - Plant Biology 2020Quote: ... were loaded onto 20 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Biochemistry 2021Quote: ... and ZORBAX SB-C18 Analytical column (4.6 x 250 mm, 5 μm particle size; Agilent), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Amino acids were separated using a VF-5ms inert 5% phenyl-methyl column (Agilent Technologies). The oven temperature was constant at 120 °C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Sections were washed with TBS-Tween 0.05% for 5 min and rabbit/mouse link (Agilent) was added to slides and incubated for 15 min at room temperature in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... sections were incubated for 5 min at room temperature with Protein Block Serum-Free (Dako). The tissue sections were then incubated with the anti-249T-P pAbs or TpMab-1 mAb (1 ...
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bioRxiv - Microbiology 2019Quote: ... followed by 5 minutes of re-equilibration at 0% B (Agilent 1290 Infinity LC system). Post-column ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were imaged using the BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies) to collect baseline GFP intensity ...
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bioRxiv - Biochemistry 2020Quote: ... or an Eclipse XDB-C18 column (150 mm x 4.6 mm, 5 μm Agilent technologies) and an in-line fluorescence detector (λex 473 nm ...
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bioRxiv - Systems Biology 2021Quote: ... were loaded onto 10 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... fitted with a guard column (2.1 mm × 5 mm, 1.8 μm Agilent ZORBAX SB-C18) was used ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-mouse IgG (1.3 μg ml−1, 5% milk in PBST; Dako, P026002-2) or goat anti-rabbit IgG (250 ng ml−1 ...
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bioRxiv - Cell Biology 2021Quote: ... were loaded onto 10 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Molecular Biology 2024Quote: ... and heated for 5 minutes (40°-70°C) in a PCR machine (Agilent SureCycler 8800). Next ...
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bioRxiv - Cell Biology 2024Quote: 96-well plates containing single-cell clones were imaged on a Cytation 5 microscope (Agilent) 10 days after sorting ...
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bioRxiv - Cancer Biology 2024Quote: ... Each cycle included a 5 min blocking step with serum free Protein Block (Agilent, #X0909), primary antibody incubation for 30 min ...
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bioRxiv - Neuroscience 2023Quote: Slide scanning and quantification were done using a Cytation 5 from Agilent (Cat No. BTCYT5PW) with GEN5PRIME software (Cat No ...