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Citations for Agilent :
701 - 750 of 1346 citations for Recombinant Human 5' Nucleotidase Ecto CD73 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... DNA genomic libraries were prepared from fresh tissue (tumor and nontumor) and FFPE tumor tissue DNA prior to exome capture (SureSelect XT Human All Exon v5, Agilent). Libraries were sequenced on a HiSeq2000 instrument (Illumina ...
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bioRxiv - Cell Biology 2024Quote: Whole exome sequencing on genomic DNA of the patient was performed using the SureSelect Human All Exon V6 kit (Agilent) on an Illumina HiSeq 4000 sequencer ...
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bioRxiv - Molecular Biology 2019Quote: ... and 5% (v/v, no unit definition available) AffinityScript Multiple Temperature Reverse Transcriptase (Agilent). RNA was incubated at 65°C for 5 min and was then left at RT for 10 min for primer annealing ...
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bioRxiv - Cell Biology 2019Quote: Cleaved Caspase-3 staining required 5 minute treatment with Target Retrieval Solution (DAKO S1700), 1 hour room temperature incubation with Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 µm-thick paraffin sections were deparaffinized and autoclaved in target retrieval solution (DAKO) for 10 min at 121°C ...
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bioRxiv - Biochemistry 2020Quote: ... The non-specific binding was blocked for 30 min with 5% rabbit serum (DakoCytomation) for the primary mouse serum(1:600) ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were blocked and incubated first with anti CD3 antibody (Dako, Supplementary Table 5) and subsequently with horseradish peroxidase conjugated anti rabbit antibody (Dako) ...
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bioRxiv - Cancer Biology 2022Quote: 5 × 104 cells were plated on XF24 cell culture plate (Agilent, no. 100777-004) with DMEM supplemented with 1 % FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Luminescence was determined using the BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent). Cell viability analysis was performed on GraphPad Prism 9 and IC50 values were determined using a nonlinear regression curve fit ...
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bioRxiv - Synthetic Biology 2022Quote: ... Both oligo libraries (Round 5, and Round 6 + mutational scanning) were purchased from Agilent.
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bioRxiv - Plant Biology 2019Quote: ... connected to a Zorbax SB-C18 analytical guard column (5-µm, 4.6 × 12.5mm; Agilent), 0.1% formic acid (v/v ...
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bioRxiv - Microbiology 2019Quote: ... equipped with a C18 reversed-phase column (4.6 × 250 mm, 5 μm; Agilent Technologies) was used for HPLC analysis ...
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bioRxiv - Cell Biology 2019Quote: ... The sections were stained as follows: 1) blocking endogenous peroxidases for 5 min (DAKO Real Peroxidase Blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... blocked with 5% skimmed milk and probed successively with anti-Flag (Agilent, 200474-21) and anti-Rat (Licor ...
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bioRxiv - Microbiology 2021Quote: ... Agent SB-Aq reverse-phase column (250 mm, 4.6 mm i.d., 5 mm; Agilent) was used to perform HPLC at 30°C ...
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bioRxiv - Cancer Biology 2022Quote: ... using the following protocol: Endogenous Enzyme Block for 5 min (FLEX Peroxidase Block, Dako), primary antibody (HER2 A0485 and Melan-A IR633 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with a HP-5 Ultra Inert column (Agilent, 30 m × 0.25 mm, 0.25 µm). 1μl of each sample was injected using Agilent ALS 7694 autosampler in split mode with a 5:1 ratio with helium as the carrier gas (250°C injector temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent images of severed axons were acquired with the Cytation 5 automated microscope (Agilent). Within one experimental replicate ...
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bioRxiv - Microbiology 2022Quote: ... and 20% glycerol at 30°C in a BioTek Cytation 5 imaging reader (Agilent) with filters for excitation at 360/40 nm and emission at 460/40 nm ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were desalted using solid phase extraction C18 cartridges (Agilent, 5 μL bed volume). Then ...
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bioRxiv - Biophysics 2023Quote: ... we constructed the 5’-biotin handles by a PCR from a pBluescript SK+ (Stratagene) using 5’-biotin primers JT337 and JT338 (Table S1) ...
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bioRxiv - Cell Biology 2023Quote: ... both packed with a Zorbax 300SB 5 μm C18 phase (Agilent Technologies, Waldbronn, Germany). The mobile phase was composed of H2O/FA (100:0.1 ...
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bioRxiv - Genomics 2023Quote: ... cell counts were measured using a Cytation 5 Cell Imaging Multimode Reader (Agilent BioTek).
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bioRxiv - Genomics 2023Quote: ... 5 ng of each RNA sample was analyzed using a 5200 Fragment Analyzer (Agilent) with the High Sensitivity RNA Assay kit (DNF-472) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-biotin handles were constructed by a PCR reaction from a pBluescript SK+ (StrataGene) using 5’-biotin primers JT337 (Bio-AGAATAGACCGAGATAGGGTTGAGTG ...
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bioRxiv - Microbiology 2023Quote: ... The fixed plates were scanned using the high-content imaging system Cytation 5 (Agilent), with either the GFP channel for rVSV-S or the Texas red channel for rSARS-CoV-2 virus ...
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bioRxiv - Cancer Biology 2023Quote: ... plates were transferred to the Cytation 5 Cell Imaging Multi-Mode Reader (Biotek/Agilent) driven by Gen5 software (Biotek/Agilent) ...
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bioRxiv - Biochemistry 2023Quote: Probe-phosphonate–labeled peptides were enriched using Fe(III)-NTA 5 μl (Agilent Technologies) in an automated fashion by the AssayMAP Bravo Platform (Agilent Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... 5 µl of this sample was injected on a HILIC-Z column (Agilent Technologies) and 13C labeled TCA intermediates were measured on an Agilent 6546 QTOF mass spectrometer that was coupled with an Agilent 1290 Infinity II UHPLC system (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... images were automatically captured at 40X magnification using the BioTek Cytation 5 (Agilent Technologies) from the Sanford Burnham Prebys Cell Imaging Core and were subjected to automated analysis using the BioTek Gen5 software14 ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with antibody polyclonal Guinea Pig anti-Human Insulin (cat. A21435 - Agilent Technologies, Santa Clara, CA, USA) diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Immunology 2022Quote: ... tissues fixed for 24-30 hours in 10% neutral-buffered formalin (NBF; 3.7% formaldehyde) as previously described (Wiarda et al., 2020) with polyclonal rabbit α-human CD3ε antibody (Dako A0452) and polyclonal goat α-rabbit horseradish peroxidase (HRP)-conjugated antibody (Dako K4003) ...
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bioRxiv - Immunology 2019Quote: ... the wells were washed as before and incubated with 50 µl/well polyclonal rabbit anti-human IgG conjugated with horseradish peroxidase (Dako, Denmark) at 0.5 µg/ml for another hour at room temperature ...
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bioRxiv - Genomics 2021Quote: ... 1.65 μg cRNA from each labeling reaction was hybridized to the Agilent Whole Human Genome Oligo Microarray (Agilent Technologies, Tokyo, Japan). The Whole Human Genome Oligo Microarray is in a 4 x 44k slide format and each block represents more than 41,000 unique human genes and transcripts ...
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bioRxiv - Genetics 2021Quote: ... and total RNA (ribosomal RNA depleted, toRNA-seq) obtained from an adult human cortex [66-year-old female (540005, Agilent Technologies)] and an adult mouse cortex [eight-week-old (C57BL/6J ...
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bioRxiv - Cancer Biology 2021Quote: Comparative gene expression profiling of the prostate cancer cells starved for glutamine for 24 h or cultured with glutamine supplementation was performed using SurePrint G3 Human Gene Expression 8×60K v3 Microarray Kit (Design ID 039494, Agilent Technologies) according to manufacturer’s recommendations as described previously 4 ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA from each clone was analyzed by microarray-based comparative genomic hybridization using catalog whole-genome microarrays (SurePrint G3 Human CGH microarray, 8 × 60 k format, Agilent technologies) and/or by multiplex ligation dependent probe amplification using the SHOX SALSA MLPA Probemix (assay ID ...
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bioRxiv - Cell Biology 2021Quote: Paraffin-embedded islet grafts within the kidneys or intact mouse or human islets were stained for insulin using polyclonal guinea pig anti-insulin antibody (Dako, A0564), and apoptosis was measured using the DeadEnd Fluorometric terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL ...
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bioRxiv - Cancer Biology 2022Quote: ... Synthesis of cDNA from isolated RNA as well as a Universal Human Reference RNA that was used as negative control or for normalization (HREF, Stratagene/Agilent # 740000) was performed using an oligo-dT primed SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: Transcriptome analysis of primary human HSC was performed by OakLabs (Hennigsdorf, Germany) using Agilent Microarray XS (Agilent Technologies, Santa Clara, USA). Low Input QuickAmp Labeling Kit (Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: Trio WES of 13 patients and their parents was performed using the SureSelect Human All Exon V5+UTR kit (Agilent technologies). In patient 4 ...
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bioRxiv - Biochemistry 2019Quote: ... carrying the human WT RAD51 (WT-pET11d) was used as a template for the PCR-mediated QuikChange site-directed mutagenesis (Agilent Technologies) to introduce F86E substitution (F86E-pET11d ...
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bioRxiv - Cancer Biology 2019Quote: ... was used for preparing the samples for sequencing and SSELXT Human All exon V6 +UTR probes (Agilent Technologies, California, United State) were used for the exome capture ...
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bioRxiv - Genomics 2019Quote: Paired-end DNA sequencing libraries of 28 individuals were generated using Aglilent SureSelect Human All ExonV6 kit (Agilent Technologies, CA, USA) by Novogene Co. ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were treated with 1:10 normal goat serum in PBS for 15 minutes and incubated for 60 minutes with 1:200 diluted Rabbit anti-human thyroglobulin (Dako, Denmark) or 1:400 diluted Rabbit anti-human calcitonin (Dako ...
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bioRxiv - Cell Biology 2020Quote: To identify the causative mutation in these families we sequenced the whole exome of the affected individuals with the SureSelect human AllExon 50Mb kit (Agilent Technologies) and sequenced on the HiSeq 2500 (Illumina ...
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bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... RNAs from 4 different experiments (biological quadruplicate) were extracted as described above and analyzed on SurePrint G3 Human Gene Expression 8×60K v2 Microarrays (#G4851B, Agilent Technologies) as previously described (36) ...
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bioRxiv - Neuroscience 2020Quote: ... Human α7-nAChR R208I-GFP and E211N-GFP were generated from pcDNA3.1-CHRNA7-mGFP and human α3-nAChR I284R-GFP and N287E-GFP were generated from human α3-nAChR-GFP by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...