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Citations for Agilent :
8101 - 8150 of 9205 citations for Mouse Autophagy related protein 16 1 ATG16L1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Whole-exome sequencing (WES) libraries were prepared using the SureSelect Human All Exon V6 kit from Agilent (Agilent Technologies, Santa Clara (CA), U.S.A. ...
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bioRxiv - Genomics 2023Quote: ... All library validations were performed on a 2100 Bioanalyzer with High Sensitivity DNA kit according to manufacturer’s recommendations (Agilent Technologies, 5067-4626).
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was determined with an RNA ScreenTape Kit on the TapeStation 2200 system (Agilent; 5067-5576, 5067-5578, 5067-5577) and only RIN scores of 8 or higher were used for sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... A sequence coding for the MAAAPQKC peptide was inserted in place of the first ten codons of the ermCL sequence using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Inc). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutants of KflaHY2 were generated in the pET28a_KflaHY2 plasmid using the QuikChange® Lightning kit (Agilent, Santa Clara, California, USA) employing primers listed in Table S1 ...
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bioRxiv - Systems Biology 2023Quote: The genomic integrated sgRNA libraries were amplified and indexed in two rounds of PCR using the Herculase II Fusion DNA Polymerase kit (Agilent Technologies 600679). The libraries were sequenced on an Illumina NextSeq with a custom primer oMCB1672 for read 1 and ≥21 cycles in read 1 ...
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bioRxiv - Genomics 2023Quote: ... cDNA was diluted to approximately 3 ng/μL and run on the 2100 Agilent Bioanalyzer using the High Sensitivity DNA Kit (Agilent 5067-4626). The measured fluorescence units in Fig 1b have been corrected for the dilution factor.
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bioRxiv - Plant Biology 2023Quote: ... Site directed mutagenesis was performed using the QuikChange Site-directed Mutagenesis Kit following the instructions of the manufacturer (Stratagene; La Jolla, CA) using the primers listed in Supplemental Table 1.
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bioRxiv - Cancer Biology 2023Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA of 100ng was subjected to reverse transcription using the AffinityScript qPCR cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A 2 µl aliquot of processed gDNA was taken to assess shearing using an Agilent High Sensitivity D1000 Kit (Agilent, Cat. #G2991AA). Once shearing was assessed ...
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bioRxiv - Systems Biology 2023Quote: ... and confirmed the quality of the RNA samples using a Fragment Analyzer (Advanced Analytical Technologies, Inc.) with the Standard Sensitivity RNA Analysis Kit (Agilent, #DNF-471).
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bioRxiv - Molecular Biology 2023Quote: ... and the size distribution of the library was assessed using an Agilent 2100 Bioanalyzer with a High-Sensitivity DNA analysis kit (Agilent, 5067-4626). DETECTOR-seq libraries were sequenced on an Illumina HiSeqX platform to a depth of 10 million 150 bp paired-end reads per sample ...
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bioRxiv - Developmental Biology 2024Quote: ... Exome capture was performed using the IDT xGen Exome Research Panel v2.0 in conjunction with library preparation using the SureSelectXT kit (Agilent Technologies, California, USA) following the manufacturer’s recommendations and incorporating Illumina® 8-nt dual-indices ...
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bioRxiv - Pathology 2024Quote: RNA integrity and quantification was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies, CA, USA). RNA purity was checked using a NanoPhotometer spectrophotometer (IMPLEN ...
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bioRxiv - Immunology 2023Quote: ... Pool quality control was performed with an Agilent High Sensitivity D1000 Screen Tape reagent kit using the Agilent 4200 TapeStation (Agilent Technologies, USA). Whole-genome sequencing (WGS ...
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bioRxiv - Cancer Biology 2023Quote: ... The labeling and hybridization steps were conducted in accordance with the provided instructions using the SureTag DNA Labelling kit (Agilent Technologies, USA). Equal amounts of DNA labeled with fluorescent markers were combined and co-hybridized onto a 44LK DNA microarray ...
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bioRxiv - Synthetic Biology 2023Quote: ... The RNA quality was evaluated using the 5200 Fragment Analyzer System and the Agilent HS RNA Kit (Agilent Technologies, Inc., CA, USA). After the ribosomal RNA depletion with the RiboPOOL kit (siTOOLs Biotech GmbH ...
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bioRxiv - Neuroscience 2023Quote: ... were used for each replicate and placed into 100 µL of lysis buffer + β-mercaptoethanol from the Absolutely RNA Microprep kit (Agilent Technologies). Each genotype was prepared in quadruplicate ...
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bioRxiv - Immunology 2023Quote: ... Mutations were introduced into cDNAs using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA, USA; cat. no. 200521) to construct expression vectors for S variants with a single amino acid change ...
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bioRxiv - Cell Biology 2023Quote: ... GYF motif mutant GIGYF1 (GIGYF1 GYF Mut; G502A, Y503A, F504A) plasmids were created using the QuikChange II Site-Directed mutagenesis kit (Agilent Technologies, 200523). The chimeric constructs were synthesized by GenScript (Piscataway ...
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bioRxiv - Physiology 2024Quote: ... for assessment of the RNA integrity by the RNA 6000 Nano Chip Kit with the Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), and samples with RIN number above 7.5 were used for micro-array analysis ...
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bioRxiv - Microbiology 2023Quote: ... and their concentrations were checked by Qubit (ThermoFischer Scientific) followed by quality assessment through Agilent 2100 BioAnalyzer (Agilent RNA 6000 Nano Kit). Both sets of RNA seq represented in Figures 1 and 5 were processed and run at the same time ...
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bioRxiv - Plant Biology 2024Quote: ... The quality of RNA was assessed using an Agilent RNA 6000 Nano Kit on the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). Only samples with an RNA integrity number (RIN ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA and library quality control were performed using an Agilent 2100 Bioanalyzer using the Bioanalyzer High Sensitivity DNA kit (Agilent #5067-4626). Sequencing was performed by Azenta Biosciences using a NovaSeq instrument.
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bioRxiv - Genomics 2024Quote: ... #Q32854) was used to assess RNA concentration and RIN values were determined using the Bioanalyzer Pico RNA kit (Agilent cat. #5067-1513), with average RIN scores of 8.2 for the adrenal gland ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA library quality and quantity were determined using an Agilent 2100 bioanalyzer using the High Sensitivity DNA Reagents kit (Agilent, 5607-4626) and then sequenced on an Illumina NovaSeq 6000 ...
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bioRxiv - Immunology 2024Quote: ... The mitochondrial function of macrophages and amastigotes was analyzed by the Agilent Seahorse XF Cell Mito Stress Test Kit (Agilent, 103015-100). The glycolytic function of infected macrophage was analyzed by the Agilent Seahorse XF Glycolytic Stress Test Kit (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... Cells used for all experiments were ≤9 passages from stock and tested negative for mycoplasma contamination (Agilent MycoSensor QPCR Assay Kit, 302107).
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bioRxiv - Immunology 2024Quote: ... and evLIMs were established by assessing glycolytic-vs OXPHOS- (mitochondrial oxidative phosphorylation) dependent ATP production using the Agilent Seahorse XF Real-Time ATP Rate Assay Kit (Agilent, 103592-100). The mitochondrial function of macrophages and amastigotes was analyzed by the Agilent Seahorse XF Cell Mito Stress Test Kit (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral vector carrying mutant SPDEF (G277D) was generated using QuikChange II XL Site-Directed Mutagenesis Kit (cat# 200521, Agilent, Santa Clara, CA) with oligonucleotides (5’-tcctcaattttgaagatgtccttctccttgttgagcc-3’ and 5’-ggctcaacaaggagaaggacatcttcaaaattgagga-3’ obtained from IDT ...
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bioRxiv - Developmental Biology 2024Quote: Individual and combination mutant versions of Homo sapiens CTNNB1 DNA constructs were generated using standard cloning techniques and the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, 200521), unless otherwise noted ...
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bioRxiv - Plant Biology 2024Quote: ... and integrity of RNA on 0.8% agarose gels (v/w) and by the RNA Nano 6000 Assay Kit using Bioanalyzer instrument (Agilent Technologies, CA, USA). For reverse transcription of approximately 1 mg of the DNAse-treated RNA ...
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bioRxiv - Plant Biology 2024Quote: ... Quality and quantity of the finished libraries were assessed using an Agilent 4200 TapeStation DNA 1000 kit (Agilent Technologies, Santa Clara, CA) and Qubit® dsDNA HS Assay Kit (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... PCR probes were labelled with [α-32P]-dCTP (Hartmann Analytic #SRP-305) using the Prime-it II Random Primer Labelling Kit (Agilent, #EK0031) and purified on illustra Microspin G-50 columns (Cytiva #27533001 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from the beads and purified according to the manufacturer’s instructions using an Absolutely RNA nanoprep kit (Stratagene, La Jolla, CA) with in column DNA digestion and frozen prior to gene expression profiling being performed ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the library profile was assessed using a High Sensitivity DNA kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The library was then sequenced to approximately 50x coverage on an Illumina HiSeq 4000 instrument (Illumina ...
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bioRxiv - Biochemistry 2024Quote: ... Introduction of mutation (ATG to TAA) into the pcDNA3.1-HA-mGluR2-mCitrine construct was performed with the QuikChange II Site-Directed Mutagenesis Kit (Agilent, Catalog no. 200523) (for primer pairs ...
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bioRxiv - Cancer Biology 2024Quote: ... and integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). 1μg RNA was used for sequencing libraries using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 ng of purified RNA was mixed with RNA standards (One Colour RNA Spike-In Kit; Agilent, Santa Clara, CA – 5188–5282) and the mixture labelled with LowInput QuickAmp Labeling Kit One-Color (Agilent 5190-2305) ...
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bioRxiv - Physiology 2024Quote: ... Library quality controls were performed by using Agilent High Sensitive DNA kit with Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and quantified by Qubit 2.0 fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... cells were resuspended in 200 μL PBS containing 1% BSA for detection by NovoCyte (Agilent) utilizing laser excitation and emission wavelengths of 488 nm and 530 nm ...
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bioRxiv - Cancer Biology 2021Quote: ... run 1 μl of sample on an Agilent Bioanalyzer High Sensitivity chip (Agilent, Cat#: 50674626) for cDNA QC & Quantification.
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with horseradish peroxidase-conjugated streptavidin (P0397; Dako; 1:3000, 3% BSA) at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μl of the odor sample was injected in a DB5 column (Agilent Technologies; www.agilent.com), fitted in an Agilent 6890 gas chromatograph ...
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bioRxiv - Genomics 2020Quote: ... DNA was eluted into 53 µl with 1 µl used for D1000 TapeStation analysis (Agilent) and 2 µl used for Qubit quantification (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The RNA was solubilized to an OD260 of 1 and analysed in the Bioanalyzer (Agilent) for quality and quantity estimation for RNA-seq ...
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bioRxiv - Pathology 2019Quote: The clinical data and level 3 mRNA microarray data (Agilent 244K TCGA Custom 1-2) of primary GBM samples were downloaded from the NCI Genomic Data Commons ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA integrity was verified on 1% agarose gel and by RNA Screen Tape assay (Agilent). cDNA was produced with High Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5 ...