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Citations for Agilent :
8001 - 8050 of 8085 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... the cell pellet was resuspended in 500ul of warm MAS buffer solution [58] + 1nM Plasma Membrane Permeabilizer (Agilent Seahorse XF PMP) to permeabilize the cells ...
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bioRxiv - Biochemistry 2024Quote: ... pT7-7 α-syn FL plasmid (a gift from Hilal Lashuel, Addgene66) was transformed into BL21-Gold (DE3) competent Escherichia coli (E. coli) cells (Agilent Technologies) according to the manufacturer’s instructions and previous methodology44 ...
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bioRxiv - Neuroscience 2024Quote: Ten µg of mitochondrial protein in a volume of 30 µl were added to each well of a Seahorse XFe96/XF Pro Cell Culture Microplates (Agilent Technologies) coated with polyethylenimine (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: Mitochondrial respiratory function in live SH-SY5Y cells was measured using the Seahorse XFe96 Extracellular Flux Analyzer (Agilent Technologies, California, USA) via changes in the Oxygen Consumption Rate (OCR ...
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bioRxiv - Neuroscience 2024Quote: ... except that the dissociated spinal cord cells were plated in 80 µL serum-containing plating medium (50% DMEM, 25 % HBSS and 25 % horse serum) at 80.0000 cells per well in Seahorse XFp Miniplates (Agilent, 103025-100). After two hours ...
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bioRxiv - Microbiology 2024Quote: ... Cell densities were determined in two technical replicates with a NovoCyte Flow Cytometer equipped with a NovoSampler Pro (Agilent, Waldbronn, Germany). Two times 200 µL of each sample were pipetted into a 96-well microtiter plate (Sarstedt ...
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bioRxiv - Biophysics 2021Quote: ... The pre-incubated LNPs (see above) were transferred to the cells using a Bravo liquid pipetting robot (Agilent Technologies, Santa Clara, US). All treatments were analysed in four technical replicates ...
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bioRxiv - Biophysics 2021Quote: Wild-type (WT) 3RS-and 4RL-Tau constructs were expressed in BL21 CodonPlus(DE3)-RP Escherichia coli cells (Stratagene, La Jolla, CA) and purified as previously described [17 ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with prediluted antibody polyclonal Guinea Pig anti-Human Insulin (cat. IR002 - Agilent Technologies, Santa Clara, CA, USA) for 1h at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were co-transfected with 100ng pCAG-Lgr6 overexpression plasmid (pLgr6) or pBluescript II KS+ (pBKS) (Agilent Technologies, Santa Clara, CA, USA) as a control ...
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bioRxiv - Cancer Biology 2020Quote: ... to extract total RNA cells from the various experimental conditions and tested for RNA quality using a Bioanalyzer (Agilent, Santa Clara, CA). Contaminating genomic DNA was removed using Turbo DNA free from Ambion ...
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bioRxiv - Systems Biology 2020Quote: Treated and control cell lysates were subjected to size exclusion chromatography (SEC) using an Agilent 1100 HPLC system (Agilent Technologies, ON, Canada) with a multi-phase chromatography protocol as previously described24 ...
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bioRxiv - Cell Biology 2020Quote: His-tagged Ran in the pET28 vector (a gift from Dr. Günter Blobel) was transformed into BL21 (DE3) RIL-competent cells (Stratagene, St. Louis, MO). Expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2022Quote: ... 150,000 cells (activated with anti-CD3 and anti-CD28 antibodies 48 hours prior) were plated into individual wells of a poly-D-lysine coated seahorse cell culture microplate (Agilent; 101085-004). T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM ...
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bioRxiv - Biochemistry 2021Quote: ... fluorescence of labeled and unstained control cells was quantified with an ACEA NovoCyte benchtop flow cytometer (Agilent Technologies, Santa Clara, CA, USA). After applying a cut-off of 7.5 × 105 to the forward scatter ...
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bioRxiv - Immunology 2019Quote: Individual subunits (Strep-Tactin and dead streptavidin-SpyCatcher or dead streptavidin and Strep-Tactin-SpyCatcherΔ) were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies # 230280) and refolded from inclusion bodies using a modified version of the protocol previously described by Howarth and Ting43 ...
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bioRxiv - Neuroscience 2022Quote: Co-transfection of pAAV-CAG-DIO-(hCAR)off-(GtACR2Kv2.1-MuGFP)on-W3SL with pDPI and pDPII plasmids (Grimm et al., 2003) into AAV293 cells (Agilent Technologies, CA, USA) preceded harvesting and iodixanol gradient purification (as described by (Zolotukhin et al. ...
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bioRxiv - Plant Biology 2022Quote: ... USA) equipped with a Cobalt cell (Teledyne Photon Machines) and connected to an Inductively Coupled Mass Spectrometer (model 8900, Agilent Technologies, USA). Analysis was done with the following settings ...
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bioRxiv - Cell Biology 2022Quote: ... Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were measured in MIN6 cells using an XF24 Analyzer (Seahorse Bioscience, MA, USA).
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bioRxiv - Neuroscience 2024Quote: ... OCR and ECAR were measured as follows: cell culture media was removed and 80µl of XF Media (Agilent Technologies; Cat #: 103334-100) containing no pH buffers and supplemented with 5.5 mM glucose ...
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bioRxiv - Neuroscience 2024Quote: ... 10K HMC3 microglia per well were seeded in 80µl of a Agilent Seahorse XF96 Cell Culture Microplate (Agilent Technologies; Cat #: 101085-004) and incubated overnight ...
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bioRxiv - Systems Biology 2024Quote: Media to be used for cultivating cells for intracellular metal quantification with ICP-MS was through a PVDF membrane plate (Agilent 200931-100) into fresh deep-well (2mL ...
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bioRxiv - Cell Biology 2024Quote: Protein expression was initiated by transforming ∼80 ng of each DNA plasmid into 50 µL of XL-1 Chemically Supercompetent Cells (Agilent Technologies, 200236) at 42 °C for 30 seconds followed by 5 minutes of incubation on ice ...
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bioRxiv - Cell Biology 2024Quote: MitoSnap+ and MitoSnap-cells were purified by FACS and their oxygen consumption rates (OCR) were measured using a XF96 MitoStress Test (Seahorse Agilent, 103015-100). Activated CD8+ T cells were washed in RPMI 1640 without sodium bicarbonate ...
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bioRxiv - Immunology 2023Quote: ... following standard protocol and plated at a concentration of 2×105 cells per well of a seahorse XFe96 assay plate in seahorse XF DMEM media (Agilent; 103575-100) supplemented with 1mM pyruvate ...
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bioRxiv - Physiology 2023Quote: ... For β-cell proliferation analysis in P1 pups every other section was labeled with guinea pig anti-insulin (1:200; DAKO A0564) and mouse anti- BrdU (1:500 Sigma B2531 ...
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bioRxiv - Neuroscience 2023Quote: ... variants of CaMKII with N-terminal His6-SUMO tag were co-expressed with λ phosphatase in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ for 24 h ...
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bioRxiv - Biophysics 2023Quote: ... The cells were fixed with 3% formalin 3 days after the transfection and analyzed with the ACEA Quanteon (Agilent NovoExpresss Version 1.5.0), as described (22 ...
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bioRxiv - Synthetic Biology 2024Quote: For FI-MS cell extracts were directly injected into an Agilent 6546 Series quadrupole time-of-flight mass spectrometer (Agilent Technologies, USA) as described previously9,10 ...
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bioRxiv - Cell Biology 2024Quote: ... and Extra Cellular Acidification Rate (ECAR) values were normalized based on cell numbers per each well using Wave software (Agilent Technologies, Inc). Data analysis and visualization were performed using GraphPad Prism (Version 9.5.1 (528)).
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bioRxiv - Microbiology 2024Quote: ... 1:2 dilution series in serum-free media was performed and cells were infected in a 96-well plate (Agilent, 204624-100) for 1 hour at 37°C followed by addition of methylcellulose (Sigma M0512-500G ...
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bioRxiv - Cancer Biology 2024Quote: Analyses of Glutamine Dependency were performed on live cells using a Seahorse XF HS Mini Bioanalyser (Agilent Technologies; Santa Clara, CA, USA) and the Mito Fuel Flex Test (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... PBMC from control SIV naïve and SIV-infected+ART treated macaques were plated into XF24 polystyrene cell culture plates (Seahorse Bioscience, North Billerica) at 20,000/well ...
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bioRxiv - Immunology 2024Quote: Strep-Tactin SpyCatcher and dead streptavidin (monovalent) or Strep-Tactin SpyCatcher and Strep-Tactin (tetravalent) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies #230280) were combined and refolded from inclusion bodies ...
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bioRxiv - Neuroscience 2024Quote: ... 50,000 cells per well were plated after 3 days of induction in a specialized Seahorse analyzer 96-well plate (Agilent, 103774-100). Cells were washed in PBS once and incubated in a hypoxic chamber following the manufacturer’s procedure ...
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bioRxiv - Cancer Biology 2021Quote: ... UACC-62 and RPE cells was characterized by extracellular flux analysis using Agilent Seahorse XF96 Analyzer (Agilent Seahorse Bioscience, Santa Clara, CA, USA), as an indicator of the functional bioenergetic capacity of the mitochondria and overall cellular health in response to TRMT61B downregulation.
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bioRxiv - Genomics 2021Quote: Exome capture was carried out for six cell lines and 39 mixtures using SureSelect Human All Exon V6 (Agilent Technologies, Inc., CA, USA). To minimize duplicate reads in ultra-deep sequencing ...
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bioRxiv - Immunology 2021Quote: ... F4/80 (Serotec MCA497GA, 1:400), wide-spectrum keratin (CKWSS, which labels bile duct epithelium and hepatic progenitor cells (Dako #Z0622, 1:400)) or SMA (Dako #M0851 ...
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bioRxiv - Cell Biology 2021Quote: ... A P/O ratio of 2.75 has been validated for calculating the OXPHOS ATP production rate in cells (Romero N et al Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology). Thus ...
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bioRxiv - Microbiology 2020Quote: ... 2008 was filtered through 3 μm and sent to Bigelow Laboratory for Ocean Sciences (Boothbay Harbor, ME, USA) for cell sorting in a MoFlo™ Flow Cytometer (Dako-Cytomation, Denmark). Digestive vacuoles of heterotrophic protists were stained using the vital stain LysoTracker® (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: Real time extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) in cells were determined using the Seahorse Extracellular Flux (XFe-96) analyzer (Seahorse Bioscience, MA, USA). 1×104 cells were seeded per well of XF96 cell culture plates and incubated for 24 h to allow adherence ...
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bioRxiv - Molecular Biology 2019Quote: ... and extracellular acidification rate (ECAR) in HKC-8 cells were studied by using a Seahorse XF24 Extracellular Flux Analyzer (Agilent, Santa Clara, CA). Key parameters of mitochondrial function were assessed by directly measuring the OCR in HKC-8 cells transfected with 40 nM of either miR-9-5p or miR-NC and treated with 10 ng/ml TGF-β1 for 48h ...
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bioRxiv - Microbiology 2019Quote: ... The reaction products for the total RNA of the human cell line KMM-1 were analysed by microfluidics-based automated electrophoresis with the Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in 100 µl of 0.5% BSA in PBS and incubated with mouse anti-human CD31 (Agilent Dako, M0823, clone JC70A) antibody-coated magnetic beads (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed 1X with 200 mL warm ATP Assay Medium prepared per protocol (Agilent XF DMEM Medium pH 7.4 (103757-100); 10 mM XF Glucose ...
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bioRxiv - Immunology 2020Quote: ... Cells cultured on 100 mm plates were washed once with PBS and cell monolayers were irradiated with 100 mJ in a UV-Stratalinker 2400 (Stratagene, San Diego, CA) with the plate lid off or on to generate the mock control ...
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bioRxiv - Cell Biology 2022Quote: Analysis of mitochondrial functions in HAP 1 WT and HAP 1 Δ(c+δ) cells was performed on Seahorse XFe24 (Agilent Technologies, USA) as described previously (Nichols et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2 × 104 NIH3T3 cells per 100 μl of growth medium were transfected with DNAs or siRNAs as described above and seeded onto a Seahorse XF96 Cell Culture Microplate (Agilent Technologies, 101085-004) for 32 h ...
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bioRxiv - Microbiology 2022Quote: ... The reduction of resazurin by viable Vero cells was measured with 530 emission and 590 excitation settings on a BioTek Synergy HTX multimode reader (Agilent, Santa Clara, CA) with Gen5 version 3.10 acquisition software ...
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bioRxiv - Cell Biology 2022Quote: ... OCI-LY-1 cells (5 × 105 cells/ml) were pretreated for 1 hour in Seahorse Seahorse XF base medium supplemented with glutamine (103334-100, Agilent Technologies, Heverlee, Belgium) in a CO2-free incubator ...