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Citations for Agilent :
7951 - 8000 of 8085 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Rod shaped adult cardiomyocytes were collected by settling down of cells and plated in 24 well plates for XF24e Extracellular Flux Analyzer analysis (Seahorse Bioscience) or on glass coverslips for confocal imaging.
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bioRxiv - Neuroscience 2020Quote: ... the original plasmid was digested with DpnI restriction enzyme and the remaining mutagenized plasmid was transformed into XL10-Gold® ultracompetent cells (Agilent). Integrity of the insert sequence and introduction of the point mutation were confirmed by Sanger sequencing of the isolated plasmid DNA.
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bioRxiv - Synthetic Biology 2021Quote: ... The molarity of the single cell libraries was calculated based on their library sizes as measured using a bioanalyzer (Agilent Technologies) and using the KAPA qPCR quantification (KAPA ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed four times with PBS containing Ca2+ and Mg2+ and mounted in a fluorescent mounting medium (DAKO, CA, USA). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time oxygen consumption rate (OCR) of these cells was measured using the Seahorse Extracellular Flux (XFe-96) analyzer (Agilent Technologies). Protein concentration of cells in each well was determined ...
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bioRxiv - Physiology 2020Quote: ... and centrifuged for 5 min at 1000 rpm and the pellet was re-suspended and plated on XF96 cell culture microplates (Cat# 101085-004, Agilent Technologies) coated with poly-D-lysine (Cat# P6407 ...
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bioRxiv - Physiology 2021Quote: ... Glutathione S-transferase (GST) hERG1a N-terminal domains or GST-only negative controls constructs were grown in BL21 (DE3) competent cells (Agilent Technologies) until they reached exponential growth ...
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bioRxiv - Cancer Biology 2020Quote: ... OVCAR5 shctrl and shFZD7 cell lines were seeded in Seahorse 96-well microplate (Agilent, Cat#102416-100, Santa Clara, CA, USA) at a density of 10-80K per well.
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bioRxiv - Developmental Biology 2021Quote: PSM cells were dissociated on day 2 of differentiation and reseeded onto fibronectin-coated Seahorse plates (Agilent cat. no. 101085-004) at a density of 7 x 105 cells per cm2 in Seahorse XF DMEM (Agilent cat ...
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bioRxiv - Immunology 2021Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured in B cells using a Seahorse XF24 Extracellular Flux Analyzer (Agilent Technologies) with kits from Agilent (Santa Clara ...
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bioRxiv - Biophysics 2020Quote: ... flow cytometry analysis was performed by using the trophoblast-specific epithelial cell marker cytokeratin 7 (Maldonado-Estrada et al., 2004) (anti-cytokeratin 7, Dako, Switzerland). Vimentin (anti-vimentin ...
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bioRxiv - Cancer Biology 2021Quote: ... we next prepared the sorted nuclei for pre-amplification-free mini-bulk/single-cell whole genome sequencing using a Bravo liquid handler platform (Agilent Technologies) as previously described (56) ...
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bioRxiv - Neuroscience 2022Quote: ... the sections or cell containing coverslips were mounted on gelatin coated slides with Fluorescent Mounting Medium (S3023, Dako Cytomation, Glostrup, Denmark). The following primary antibodies were used ...
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bioRxiv - Bioengineering 2022Quote: Cells were equilibrated 45 min before metabolic characterization in initial volume 150 μL Seahorse XF base medium (103334-100, Agilent Technologies) containing additional (in mmol L−1 ...
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bioRxiv - Bioengineering 2022Quote: Cells were dissociated from differentiation wells and seeded at 8 × 104 cells well−1 in CM support medium on Matrigel-coated XFe96 monolayer plates (Agilent Technologies). Culture medium was changed the next day to the well’s respective treatment ...
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bioRxiv - Cancer Biology 2022Quote: ... General mitochondrial function was assessed based on the Seahorse XF Cell Mito Stress Test protocol (Agilent Technologies, Santa Clara, CA, USA). Basal oxygen consumption rate (OCR ...
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bioRxiv - Microbiology 2024Quote: ... however with GFP Mouse mAb as a primary antibody diluted 1:1000 in PBST (Cell Signalling, 4B10) and Anti-mouse peroxidase (Dako, P0447) as a secondary antibody diluted 1:5000 ...
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bioRxiv - Cell Biology 2024Quote: ... RPTECs were plated at 4 x 105 cells/well in a Seahorse 24-well V7 microplate (Seahorse Bioscience, Santa Clara, CA) and cultured in complete renal epithelial growth medium for 16-18 h in a 5% CO2 incubator at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... IHC antibodies: rabbit anti-EGFR (clone SP84, Cell Marque #14R-15) at 1:25 and mouse anti-Ki67 (clone MIB1, Dako #M7240) at 1:50 with ME Kit ...
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bioRxiv - Evolutionary Biology 2024Quote: The prepared carbon dots were studied using UV−vis (1 cm cell quartz cuvette) in a Cary 100 UV−vis spectrophotometer (Agilent Technologies) in DI water ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in primary antibody (anti-Alk1 Rabbit Abcam #ab68703, 1:100; anti-CD31 Mouse Dako #M0823, 1:500) over-night at 4°C ...
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bioRxiv - Immunology 2024Quote: ... were sequentially used to treat cells during real-time measurements of extracellular acidification rate (ECAR) using Seahorse XFe24 Analyzer (Seahorse Bioscience). For the mito stress test ...
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bioRxiv - Biochemistry 2024Quote: ... Specially designed polystyrene tissue culture-treated 96-well microplates with a clear flat bottom (Seahorse XF96 V3 PS Cell Culture Microplates; Agilent Technologies) were then seeded with 80 µl of cell suspension (4.4×105 cells/ml ...
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bioRxiv - Molecular Biology 2023Quote: Oxygen consumption and Extracellular acidification rates (OCR and ECAR respectively) were measured in MDA-MB-231 cells using the XF96 Extracellular Flux analyzer (Seahorse Bioscience). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were rinsed three times with phosphate-buffered saline and mounted with a fluorescence mounting medium (S3023; Dako, Agilent Technologies). Images were captured using a laser scanning microscope (BZ-9000 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were rinsed three times with phosphate-buffered saline and mounted with a fluorescence mounting medium (S3023; Dako, Agilent Technologies). Images were captured using a laser scanning microscope (BZ-9000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed thrice with 0.1% (v/v) tween-20 / PBS and incubated in Serum Free Protein Block (DAKO, cat.#X0909) for 1h at 21°C ...
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bioRxiv - Immunology 2023Quote: The oxygen consumption rate (OCR) of treated cells was measured using an Xfe96 Extracellular Flux analyzer (Agilent, Santa Clara, CA, USA). One day before the experiment ...
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bioRxiv - Neuroscience 2023Quote: ... of VM cells cultured with the PIEZO1 antagonist GsMTx4 and the PIEZO1 agonist Yoda1 was measured using the Seahorse Cell Mito Stress Test with the extracellular flux Seahorse XFp analyser (Agilent®), in order to assess the mitochondrial respiration and glycolysis ...
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bioRxiv - Immunology 2023Quote: Real-time analysis of oxygen consumption rates (OCR) were measured in THP-1 cells using XF24/96 Extracellular Flux Analyzer (Seahorse Bioscience). Cells were seeded in Seahorse XF96 cell culture microplate (#101085-004 ...
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bioRxiv - Biochemistry 2023Quote: The concentration of iron in the cell cultures was determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES) (Agilent, 5110). Freeze-dried cell samples were digested in concentrated nitric acid at 80 °C overnight.
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bioRxiv - Systems Biology 2023Quote: The kinetic data in this study are published and were acquired from xCELLigence assays using the xCELLigence Real Time Cell Analysis (RTCA) Single Plate device (Agilent Technologies) (Dankwa et al ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Immunology 2023Quote: ... 1.5 µM FCCP and 1 µM Rotenone/1 µM Antimycin A from Seahorse XF Cell Mito Stress Test (103015-100, Agilent Technologies), and read using Seahorse XFe96 Analyzer (Agilent Technologies) ...
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bioRxiv - Biophysics 2022Quote: ... Hela CD95KO and Hela WT stable CD95-mEGFP was assessed using the QIFIKIT® for quantification of cell surface antigens by flow cytometry (K007811-8, Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: The real-time measurements of OCR of H460-FASN-WT/-KO cells were detected on a Seahorse XF24 Extracellular Flux Analyzer (Agilent Technologies). 1×10^4 cells/well were incubated with XF assay medium (pH 7.4 ...
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bioRxiv - Cancer Biology 2023Quote: Murine glioma cells (similar low passage N1IC-1/2 and p53-1/2) were seeded in PLL coated XFe24 cell culture microplates (Agilent TEchnologies) at 1.8ᵡ104 cells per well in 250μL BFP (n=10 technical replicates for the baseline experiment and n=5 technical replicates for the cysteine/methionine deprivation experiment ...
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bioRxiv - Genomics 2023Quote: Cells were allowed to grow to confluency and UV crosslinked using 254nm UV light in Stratalinker 2400 on ice (Stratagene, USA). UV treated cells were lysed to get different cellular fractions (Cytoplasmic ...
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bioRxiv - Cancer Biology 2023Quote: ... HMLER CD24low were seeded at a density of 104 cells per well in a specialized 96-well Seahorse XF96 V3 PS microplate (101085-004, Agilent Technologies). 48h after ...
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bioRxiv - Cancer Biology 2024Quote: ... and proton efflux rates (OCR/ECAR/PER) in media immediately surrounding adherent cells cultured in an XFe24-well microplate (Seahorse Bioscience). One day before analysis ...
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bioRxiv - Neuroscience 2024Quote: ... pH change) were measured over a confluent cell monolayer of HMC3 microglia using the XFe96 Seahorse extracellular flux analyzer (Agilent Technologies). First ...
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bioRxiv - Microbiology 2023Quote: ... all cultures were diluted in EMM(561 mM NH4Cl)+Leu+Ade+ura to seed an OD600nm at 0.01 into a poly-lysine coated Seahorse XFp cell culture miniplate in 50 μL of Seahorse XF assay media (Agilent Technologies). For background measurement ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated with primary antibodies (chicken anti-Map2 [Thermo Scientific, PA1-16751, 1:1000] and rabbit anti-Gfap [Dako, Z0334 ...
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bioRxiv - Biochemistry 2023Quote: ... A total of 100,000 cells were counted at each experimental point using a NovoCyte Flow cytometer (Agilent, Santa Clara, CA, USA). DHR-stained cells were detected using 488 nm for excitation and 530/30 nm for emission.
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bioRxiv - Cancer Biology 2023Quote: ... The aqueous samples were resuspended in 35x the packed cell volume using 80:20 methanol:water and transferred to polypropylene mass spectrometry vials (Agilent, 5188-2788).
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bioRxiv - Cancer Biology 2023Quote: OXPHOS and glycolysis of leukemia cells were assessed by extracellular flux using a Seahorse XFe96 Analyzer (Agilent Technologies, Santa Clara, CA) with a Cell Mito Stress Test Kit (103015 ...
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bioRxiv - Cell Biology 2023Quote: Mitochondrial respiration (JO2) of intact cells were measured using an XF HS mini Analyser Extracellular Flux Analyzer (Seahorse Bioscience, Copenhagen, Denmark). L6 myoblasts were seeded and differentiated in Seahorse XFp culture plates coated with matrigel and assayed after incubation at 37°C without CO2 for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA from FACS-purified cells was quantified and assessed for integrity using a Bioanalyser 2100 and a RNA 6000 pico assay (Agilent Technologies). RNA was processed using a NEBNext Ultra II Directional RNA library Prep Kit for Illumina paired with a Poly(A ...
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bioRxiv - Microbiology 2024Quote: ... Cell densities were determined in two technical replicates with a NovoCyte Flow Cytometer equipped with a NovoSampler Pro (Agilent, Waldbronn, Germany). Two times 200 µL of each sample were pipetted into a 96-well microtiter plate (Sarstedt ...