Labshake search
Citations for Agilent :
751 - 800 of 808 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... and the plates were incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
-
bioRxiv - Neuroscience 2022Quote: ... and the plate was incubated at 37°C for 30 minutes before reading at 562 nm on a Synergy plate reader (Agilent, Santa Clara, CA). Using the resulting protein concentration ...
-
bioRxiv - Neuroscience 2023Quote: ... Afterwards the sections were incubated in a humidified chamber (overnight 4°C) in a peroxidase conjugated immunoglobulin against UEA (DAKO, P289; 1:50). Finally ...
-
bioRxiv - Neuroscience 2023Quote: ... Antigen □ retrieval was performed by incubating the tissue for 2 h at 70 °C in Target Retrieval Solution (Dako, Santa Clara, CA, USA) using a 1:10 dilution in demineralized water ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
-
bioRxiv - Synthetic Biology 2022Quote: ... the oxidation of NADH was followed at 340 nm and 30 °C on a Cary 60 UV-Vis photospectrometer (Agilent, Santa Clara, USA) in quartz cuvettes with a pathlength of 10 mm (Hellma Optik GmbH ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and then optical density at 600 nm (OD600) was monitored with a Biotek Synergy H1M at 30°C for 48 hours with fast continuous shaking (Agilent, Santa Clara, CA).
-
Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated at 37°C in a non-CO2 incubator for 1 hour and subsequently loaded into the Seahorse XFe96 Analyzer (Agilent Technologies, Seahorse Bioscience) when prompted ...
-
bioRxiv - Cell Biology 2023Quote: ... the fibers were placed in an incubator at 37 °C with no CO2 and the medium was replaced with XF Base media (Agilent, Santa Clara, CA) with glucose (10 mM) ...
-
bioRxiv - Cell Biology 2023Quote: ... the fibers were placed in an incubator at 37 °C with no CO2 and the medium was replaced with XF Base media (Agilent; Santa Clara, CA), with sodium pyruvate (1 mM ...
-
bioRxiv - Evolutionary Biology 2023Quote: Enzymes were assayed at 25 °C by recording the change in absorbance at 340 nm for 300 seconds on a Cary 100 Bio (Agilent, Santa Clara, CA) in 50 mM Tris ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and left to grow at 30 °C and 600 rpm until all cultures reached OD600 above 0.3 measured in a Biotek Synergy H4 Multimode (Agilent, Santa Clara, CA, USA) microplate reader ...
-
bioRxiv - Microbiology 2023Quote: ... Plate was shaken continuously at 30°C and OD600 was read every 20 minutes in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent, Santa Clara, CA). MMC (0.5 μg/ml ...
-
bioRxiv - Bioengineering 2024Quote: ... and the plates were incubated at 37°C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
-
bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 1h at RT before overnight incubation at 4°C with primary antibodies: Glial Fibrillary Acidic Protein (GFAP) (1/500 Dako Cat. Number Z0334); AQP4 (1/500 ...
-
bioRxiv - Plant Biology 2024Quote: ... We used a Zorbax SB-C18 5 μm 4.6 x 250 mm column at 35°C on a 1260 Infinity HPLC instrument with a fluorescence detector (Agilent Technologies, Santa Clara CA) using an excitation wavelength of 340 nm and emission wavelength of 510 nm ...
-
bioRxiv - Developmental Biology 2024Quote: ... carefully dried using paper tissues and immediately analyzed using an Agilent Technologies GC system (7890 A GC System and 5975 C inert XL MSD with Triple-Axis-Detector, Agilent Technologies Deutschland GmbH) (Potter et al ...
-
bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
-
bioRxiv - Biochemistry 2024Quote: Spectra of dark-adapted AsLOV2 and NcVVD variants were acquired at 22°C on a diode-array absorbance spectrophotometer (Agilent Technologies, model 8453) equipped with Peltier temperature control (Agilent Technologies ...
-
bioRxiv - Bioengineering 2024Quote: ... and the plate was incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 48 hours (Fig ...
-
bioRxiv - Microbiology 2024Quote: ... of 0.1 and incubated for 100 h at 30 °C with linear shaking at 567 cycles per minute using an EPOCH microplate reader (Agilent, Santa Clara, CA). The OD600 was measured continuously.
-
bioRxiv - Cell Biology 2020Quote: ... followed by overnight incubation at 4 °C with α-α-SMA (1:400, mouse monoclonal, clone 1 A4, DAKO Agilent Technologies, Santa Clara, USA) or α-CD68 (1:2000 ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by overnight incubation at 4 °C with α-α-SMA (1:400, mouse monoclonal, clone 1 A4, DAKO Agilent Technologies, Santa Clara, USA) or α-CD68 (1:2000 ...
-
bioRxiv - Systems Biology 2019Quote: ... 20% of the total mixture was incubated at 37°C with PNGaseF and fractionated into 16 high pH reversed phase fractions using a 1260 Infinity II HPLC (Agilent Technologies, Santa Clara, CA) with a 4.6 × 150 mm XBridge C18 column and a 30 minute gradient (mobile phase A ...
-
bioRxiv - Molecular Biology 2019Quote: ... of each lipid class were prepared by acid-catalyzed transesterification at 50°C for 16 hours (Christie, 1973) before quantified by a Agilent 7890B gas chromatograph with flame ionization detector (Agilent Technologies, Santa Clara, CA).
-
bioRxiv - Cell Biology 2020Quote: ... Germany) followed by overnight incubation at 4 °C with specific primary antibodies: polyclonal rabbit anti-human AAT (1:800) (DAKO A/S, Glostrup, Denmark), mouse monoclonal anti-AAT polymer antibody (clone 2C1 ...
-
bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
-
bioRxiv - Biochemistry 2020Quote: ... linear gradient from 5% to 35% ACN in 50 mM TEAAc pH 7 over 15 min at 50 °C, Agilent Technologies Series 1200 HPLC). The collected fractions were freeze dried 3 times ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4°C in primary antibody (2% NGS in TBST) with either rabbit anti-GFAP (1:2000, Agilent Cat# Z0334, RRID: AB_10013382), rabbit anti-synaptophysin (IgG ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasmid DNA mutations in the C-terminus of PAR2 were created using QuikChange XL Multi Site-Directed Mutagenesis kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
-
bioRxiv - Physiology 2021Quote: ... The cells were incubated in this medium for 45 minutes at 37°C in a non-CO2 incubator and then transferred to a Seahorse XFe24 extracellular flux analyser (Agilent, Santa Clara, CA, USA) maintained at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... and fluorescence intensity was measured at 340/440 nm over 1 hr at 37 °C using a BioTek Synergy HTX (Agilent, Santa Clara, CA, USA).
-
bioRxiv - Cell Biology 2022Quote: ... The fluorogenic substrate was added to each well of a 96-well plate and the fluorescence intensity was measured at 340/440 nm over a 1 hr period at 37 °C using a Bio-Tek Synergy HTX (Agilent, Santa Clara, CA, USA) plate reader.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The well plate was then inserted into the humidified chamber at 37°C of an Agilent Lionheart FX Automated Microscope (Agilent Technologies, Santa Clara, CA). A band pass filter (Thorlabs ...
-
bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with FSCN1 primary antibodies at 4°C overnight and the color of the sample was developed using the DAKO ChemMate Envision Kit HRP (Dako-Cytomation, Carpinteria, CA, USA) followed by counterstaining with hematoxylin ...
-
bioRxiv - Cell Biology 2023Quote: ... at maximum speed for 10 minutes at 4°C prior to injection into a hydrophilic interaction (HILIC) liquid chromatography (LC) and high-resolution mass spectrometer (QTOF-6545, Agilent, Santa Clara, CA, USA). Metabolite identification and quality checks for polar metabolomics was performed using QTOF MassHunter Quant software (Agilent ...
-
bioRxiv - Cell Biology 2023Quote: ... Tissue sections on slides were baked at 60°C for 1 h and then deparaffinized and retrieved on Dako Omnis fully automated IHC platform (Agilent, Santa Clara, CA, USA) using EnVision FLEX target retrieval solution ...
-
bioRxiv - Cancer Biology 2023Quote: ... The analytes were separated on a Sciex C18 column (4.6 mm × 150 mm, 5 μm) maintained at 50°C using a 1260 Infinity HPLC instrument (Agilent Technologies, Santa Clara, CA, USA). A gradient flow of the mobile phase was applied ...
-
bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibodies diluted in blocking solution were incubated overnight at 4°C (anti-GFAP z0334 DAKO 1:400, anti-Iba1 019-19741 Wako 1:400). After three washes in PBS ...
-
bioRxiv - Microbiology 2019Quote: ... An aliquot of the water sample was frozen at -20°C and analysed in triplicate for metal content using ICP-MS on an Agilent 7700x (Agilent Technologies, Santa Clara, Ca, USA) at the Camborne School of Mines laboratory at the University of Exeter.
-
bioRxiv - Physiology 2019Quote: ... tissues were incubated with primary antibody over night at 4°C (guinea pig anti-insulin at 1:750, DAKO; diluted in 1% goat serum/PBS). This was followed by 1-hour incubation with fluorophore-conjugated secondary antibodies (Cy3-anti-guinea pig ...
-
bioRxiv - Biochemistry 2019Quote: ... The mixtures of standards were dried under vacuum using a vacuum rotation evaporator (Extractor Plus®, Agilent, settings: 30°C, 4 h, 1000 rpm, VAQ).
-
bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
-
bioRxiv - Biochemistry 2020Quote: ... The samples were incubated for at least 20 min at 25°C before 10 μl were injected onto a SEC-3 HPLC column (300 Å pore size; Agilent Technologies, Santa Clara, CA) equilibrated with SEC buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... slides were incubated with K10 for 30 minutes (32°C) and reactions were visualized using EnVision™ FLEX/HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) following the recommendation of the manufacturer ...
-
bioRxiv - Molecular Biology 2020Quote: ... and aromatic 1H-13C-NOESY-HSQC experiments with a mixing time of 150 ms each were carried out at 25 °C on a 500 MHz Agilent DirectDrive 2 spectrometer (Agilent Technologies, Santa Clara, CA, USA) equipped with a room temperature probe ...
-
bioRxiv - Cell Biology 2022Quote: ... from mitochondrial samples (0.150 mg/mL) in the respiration buffer under constant stirring at 28°C using a Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies, Santa Clara, CA, USA) as described previously (Emaus et al. ...
-
bioRxiv - Cancer Biology 2022Quote: ... explants treated with BrdU substrate for 24 hours prior to fixation as described in Section 6.3.1) (DAKO, Cat. M0744; 1:50 overnight at 4 °C), cytokeratin (DAKO ...