Labshake search
Citations for Agilent :
651 - 700 of 808 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... The DNA was denatured at 95°C for 5 min in a PCR machine (PTC-200, MJ Research; PCR strip tubes (Agilent 410022)) and then incubated with the biotin capture RNA at 65°C ...
-
bioRxiv - Systems Biology 2021Quote: ... using an Aminex HPX-87H ion-exchange column operated at 60°C with 5 mM H2SO4 as the mobile phase with a flow rate of 0.6 mL min-1 (Agilent, Santa Clara). The OD660 was measured with a Jenway 7200 spectrophotometer (Jenway ...
-
bioRxiv - Neuroscience 2020Quote: ... Heat antigen retrieval was performed by steaming at 98°C in target retrieval solution pH 6.1 (Dako, Carpinteira, CA, USA #S1699) for 30 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: ... The sections were incubated with a primary antibody at 4°C overnight and with a rabbit/mouse HRP-conjugated secondary antibody (Dako, Denmark) at room temperature for 2 h ...
-
bioRxiv - Microbiology 2020Quote: ... for 2hrs at 40°C and then incubated over-night at 4°C on a rotating wheel with 2 μg of anti HBc antibody (Dako B0586). Immune-complexes were captured with protein A/G magnetic beads ...
-
bioRxiv - Cancer Biology 2020Quote: ... slides with PCa tissue samples (n=144) were incubated at 60°C for 2 hours followed by target retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
-
bioRxiv - Biophysics 2021Quote: ... then was injected to online SEC-SAXS equipped with a temperature-controlled (15 °C) silica-based SEC column (Agilent BioSEC-3)32 ...
-
bioRxiv - Cancer Biology 2022Quote: ... heat mediated antigen retrieval was performed for 3 min at 125°C in citrate pH 6.0 target retrieval solution (Dako Cat# S2369) using a decloaking chamber (Biocare Medical) ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated overnight at +4 °C with primary antibodies diluted in the same solution or in an antibody diluent (Agilent #S3022). The samples were then washed in 0.1 % Triton X-100 or 1.0 % Tween20 in PBS for 30-60 minutes ...
-
bioRxiv - Biophysics 2022Quote: ... The samples were loaded on a Superdex 200 10/300 Increase column (Cytiva) at a temperature of 20°C run by a 1260 Infinity II HPLC (Agilent Technologies) at 0.6 ml/min ...
-
bioRxiv - Biochemistry 2022Quote: ... Splitless injection (injection temperature 270 °C) onto a 30 m + 10 m × 0.25 mm DB-5MS + DG column (Agilent J&W) was used ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
-
bioRxiv - Molecular Biology 2021Quote: ... Antigen concentration was determined using a PLRP-S column (2.1 x 150 mm, 300Å, 3µm) operated at 0.6 mL/min and 80°C (Agilent Technologies, Santa Clara, CA) on an HPLC equipped with a diode array detector set for absorbance detection at 214nm and ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The fragment size distribution and concentration of the final PacBio and Dovetail Omni-C library were assessed using a TapeStation (Agilent Technologies) and a Qubit Fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... 3 μm paraffin-embedded tissue sections were either dewaxed and subjected to antigen retrieval treatment with Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent) or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005 ...
-
bioRxiv - Neuroscience 2021Quote: Paraffin-embedded sections were deparaffinized and autoclaved at 121°C for 20 min in Target Retrieval Solution (Dako Japan Inc., S1700). Frozen sections were briefly washed in PBS and incubated with 0.5 % SDS (v/v ...
-
bioRxiv - Genetics 2022Quote: ... and incubated in the dark overnight at 4 C before running on a Novocyte Quanteon flow cytometer using NovoExpress 1.3.0 software (Agilent, Santa Clara, CA) at a flow rate of 14 uL/minute ...
-
bioRxiv - Cell Biology 2022Quote: ... Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured at 37°C on an XF Extracellular Flux Analyzer (Agilent Technologies) using the Cell MitoStress Test Kit (Seahorse Bioscience) ...
-
bioRxiv - Developmental Biology 2019Quote: ... using gasket slides and a hybridization chamber and incubated for 17 h at 65°C and 10 rpm in the hybridization oven (Agilent Technologies). Slides were washed with Triton X-102 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Splitless injection (injection temperature 270°C) onto a 30 m + 10 m × 0.25 mm DB-5MS+DG column (Agilent J&W) was used ...
-
bioRxiv - Cancer Biology 2020Quote: ... Splitless injection (injection temperature 270°C) onto a 30 m + 10 m × 0.25 mm DB-5MS+DG column (Agilent J&W) was used ...
-
bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated overnight at 4°C and appropriate HRP-conjugated secondary antibodies (DAKO, Agilent Technologies, Santa Clara, CA, USA) for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... Prior to immunohistochemistry antigen retrieval was performed using Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent). Quenching of endogenous peroxidase was performed by a 10 min incubation with Peroxidase-Blocking Solution (Dako REAL S2023) ...
-
bioRxiv - Cell Biology 2020Quote: ... the cells were incubated at 37°C and the medium was replaced with 180 μl XF assay medium (#102365-100, Seahorse Bioscience) containing 10 mM glucose ...
-
bioRxiv - Cancer Biology 2020Quote: ... at 4°C overnight followed by incubation with Dako EnVision+System-HRP Labelled Polymer Anti-Mouse (Aglient Dako, Cat # K400111-2) for 60 min at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... Antigen concentration was determined using a PLRP-S column (2.1 x 150 mm, 300Å, 3μm) operated at 0.5 mL/min and 60°C (Agilent Technologies, Santa Clara, CA). Buffer A was 0.1% (v/v ...
-
bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...
-
bioRxiv - Molecular Biology 2019Quote: ... Each Hi-C library (750 ng) was hybridized and captured individually using the SureSelectXT Target Enrichment System reagents and protocol (Agilent Technologies). After library enrichment ...
-
bioRxiv - Cell Biology 2021Quote: ... Splitless injection (injection temperature 270 °C) onto a 30 m + 10 m × 0.25 mm DB-5MS + DG column (Agilent J&W) was used ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Desorption was performed at 250 °C for 1 minute (splitless mode) in the injection port of a 6890N gas chromatograph (Agilent Technologies). After desorption ...
-
bioRxiv - Developmental Biology 2021Quote: ... for 6 minutes (30 seconds ON, 30 seconds OFF) at 4°C to obtain ~200 bp fragments (confirmed using an Agilent Bioanalyzer). Sonicated DNA was extracted using ethanol precipitation ...
-
bioRxiv - Systems Biology 2022Quote: ... An Agilent 1200 Series HPLC system was used with a refraction index detector at 50°C (Agilent, Santa Clara, CA, USA).
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed twice and incubated for 1 h at 37°C in the non-CO2 chamber with 180 μl of the assay medium (Agilent; 103575) supplemented with L-glutamine (2 mM ...
-
bioRxiv - Cell Biology 2022Quote: ... treated cells were then equilibrated in seahorse XF base medium supplemented with 1g/L glucose at 37°C in non-CO2 incubator for 1 hour prior to analysis using a Seahorse XF24 Extracellular Flux Analyzer (Seahorse Bioscience).
-
bioRxiv - Physiology 2022Quote: ... sections were dewaxed and epitope retrieval was performed using Citrate buffer pH6 for α-SMA and Ter119 or Tris- EDTA buffer pH9 for HMOX1 in all cases for 20 min at 97°C using a PT Link (Dako, Agilent) or with ER2 buffer (AR9640 ...
-
bioRxiv - Immunology 2022Quote: ... for 120 s and separated by a C-18 column (Zorbax 300SB 2.1 × 50 mm, 3.5 μm particle diameter, Agilent, Santa Clara, CA). For LC ...
-
bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope retrieval (HIER) was performed by immersing the tissue sections at 98°C for 20 minutes in LowFlex (Dako #K8005). Staining was performed following the epitope retrieval process using VectaStain Kit from Vector Labs for cleaved Caspase-3 and horseradish peroxidase-labeled polymer from Dako (K4001 ...
-
bioRxiv - Immunology 2022Quote: ... the tissue sections for IMC were heat pre-treated using a target retrieval solution for 30 min at 95°C (K8000, EnVision flex target retrieval solution, pH9 (Agilent Technologies). Slides were cooled down for 20 min at room temperature before rinsing with water and PBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... GC-MS analysis was performed using an Agilent 7890 A GC coupled to an Agilent 5975 C inert XL Mass Selective Detector (Agilent Technologies). A sample volume of 1 μL was injected into a Split/Splitless inlet ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated at 37°C in a non-CO2 incubator for 1 hour and subsequently loaded into the Seahorse XFe96 Analyzer (Agilent Technologies, Seahorse Bioscience) when prompted ...
-
bioRxiv - Genomics 2023Quote: The fragment size distribution and concentration of the final PacBio and Dovetail Hi-C libraries were assessed using the TapeStation (Agilent Technologies) and the Qubit Fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... Tumor xenograft slides were incubated at 60 °C for 2 h followed by antigen retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
-
bioRxiv - Molecular Biology 2023Quote: ... desorption was performed at 250°C for 1’ (splitless mode) in the injection port of a 6890 N gas chromatograph (Agilent Technologies) coupled to a 5975B mass spectrometer (Agilent Technologies) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed in a Tris-EDTA buffer for 30 min at 95°C for Ki-67 (1:200, Dako, M7240), ATRX (1:400 ...
-
bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
-
bioRxiv - Cancer Biology 2022Quote: ... were constructed from pcDNA3.1+-C-(K)-DYK-EBP plasmids using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, #210519) following the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: All absorption and emission measurements for both labeled A2AAR and Rec were carried out at 25 °C on a Synergy H4 (Agilent BioTek) plate reader with Take3 Microvolume Plates ...
-
bioRxiv - Bioengineering 2023Quote: ... at 40°C with an InfinityLab Poroshell 2.7 µM EC-C18 pre- column (3 x 5 mm; Agilent Technologies, Waldbronn, Germany) was used ...
-
bioRxiv - Neuroscience 2023Quote: ... for 60 min and incubated overnight at 4°C with primary antibodies diluted in DAKO Antibody Diluent (Agilent, cat. #S080983-2). Sections were washed in PBS (3 x 5 min ...
-
bioRxiv - Neuroscience 2023Quote: Vibratome cut (free floating) tissue was heated to 70°C in 1x Daco Target Retrieval solution (DakoTarget Retrieval Solution, pH 6.1, Dako, code. S2367) for 15 min and left to cool at room temperature (RT) ...