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Citations for Agilent :
7501 - 7550 of 8085 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were tested regularly for mycoplasma using the PCR-based assay (Stratagene, San Diego, CA, USA).
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bioRxiv - Plant Biology 2020Quote: In vivo absorption spectrum: TDX16-DE cells were scanned with an ultraviolet-visible spectrophotometer Cary 300 (Agilent, USA).
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bioRxiv - Immunology 2019Quote: ... cells were resuspended in mito stress test media with or without ligand(s) (XF base media (Seahorse Bioscience) supplemented with 1mM sodium pyruvate (Sigma) ...
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bioRxiv - Physiology 2020Quote: ... starved cells were washed and incubated with Seahorse XF Base medium Minimal DMEM (Cat# 102353-100, Agilent Technologies) supplemented with 2.5 or 0.5 mM glucose and 0.5 mM carnitine in a non-CO2 37°C incubator ...
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bioRxiv - Biochemistry 2021Quote: NIH 3T3 cells were transiently transfected with pFA2-cJunWT (PathDetect c-Jun trans-Reporting System from Agilent Technologies), or the indicated pFA2-cJun mutants or pFC2-dbd (empty vector) ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in HNO3 and metal composition was quantified using a 7900 ICP mass spectrometer (Agilent). Metal concentrations were then normalized to total protein content determined by the bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
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bioRxiv - Cancer Biology 2022Quote: 2×104 – 4×104 cells per well were plated onto a Seahorse XFe96 FluxPak plate (Agilent, 102416-100). On the day of the assay ...
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bioRxiv - Bioengineering 2022Quote: ... Cells that were attached to the culture dishes were trypsinized and counted on a NovoCyte flow cytometer (Agilent).
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bioRxiv - Biophysics 2022Quote: A plasmid encoding 6xHis-tagged methionyl-tRNA synthetase (MetRS) was transformed into BL21 (DE3) Codon+ RIL cells (Agilent). Overnight cultures were diluted (1:100 ...
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bioRxiv - Biophysics 2022Quote: Octahistidine-tagged Sec14 (His8-Sec14) was purified from BL21-CodonPlus (DE3)-RIL cells (Stratagene, La Jolla, CA, USA) as described for Sfh1 (Schaaf et al. ...
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bioRxiv - Immunology 2022Quote: ... cells were irradiated with 0.15 J/cm² of 365 nm UV light in a Stratalinker 2400 crosslinker (Stratagene) and harvested ...
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bioRxiv - Microbiology 2022Quote: ... The optical density (OD) was measured at 405nm using Cytation™ 3 Cell Imaging Multi-Mode Reader (Agilent) and data was analysed using BioTek Gen5 software (Agilent) ...
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bioRxiv - Neuroscience 2022Quote: ... IMGLs were seeded as a monolayer with 50 000 cells/well in a XF96-well Seahorse microplate (Agilent). Cells were washed and equilibrated in the XF Assay modified DMEM medium for 1 h at 37°C non-CO2 incubator ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells were then washed in 1X PBS and mounted using Dako mounting media (Cat#S3023, Agilent, USA).
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bioRxiv - Developmental Biology 2024Quote: ... 2009) (1μg) were co-transfected into H9 hESC (70,000 cells cm-2) using GeneJammer transfection reagent (Agilent Technologies). To enrich for successfully transfected cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated in 500 µL Seahorse XF Base Medium Minimal DMEM without Phenol Red (Agilent, 103335-100) 1 h at 37 °C in CO2 free-atmosphere ...
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bioRxiv - Cell Biology 2024Quote: ... and cells were washed twice with 200 µL of the respective assay medium for mito-stress test (Agilent Base Medium supplemented with 1mM pyruvate ...
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bioRxiv - Cell Biology 2024Quote: Myotubes were cultured in 96-well Seahorse XF cell culture microplates (#103015-100, Seahorse Bioscience, Agilent Technologies, US). Before running the assay ...
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bioRxiv - Cell Biology 2024Quote: Myotubes were cultured in 96-well Seahorse XF cell culture microplates (#103015-100, Seahorse Bioscience, Agilent Technologies, US). Before running the assay ...
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bioRxiv - Genomics 2024Quote: ... the pETM11-Sumo3-Tn5(E54K,L372P) plasmid was transformed into Escherichia coli BL21(DE3) codon + RIL cells (Stratagene). Cells were grown in TB-FB media (1.2 % Bacto-Tryptone ...
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bioRxiv - Cancer Biology 2023Quote: ... individual stained coverslips with cells were carefully transferred with tweezers to slides with prepared mounting medium (S3023, DAKO). Fluorescence detection of ORF1p and ORF2p was performed the day after the mounting medium dried using a ZEISS 700LSM confocal microscope with plan-apochromat 40x oil objective lens ...
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bioRxiv - Cancer Biology 2023Quote: ... 30,000 LM7 or LM7-KO cells were added to each well of a 96 well E-Plate (Agilent) in complete RPMI ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were re-fed every four days using an Agilent Bravo liquid handler (Agilent Technologies, Santa Clara, CA). Following a 72-96h or 10 day incubation at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: NPCs were seeded at a density of 50,000 cells per well into Seahorse XF96 96-well plates (Agilent), and were differentiated into neurons as described above ...
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bioRxiv - Cell Biology 2023Quote: ... the cell maintenance medium was exchanged with XF DMEM Base Medium (pH 7.4) with no phenol red (Agilent) supplemented with 1mM sodium pyruvate (Corning Cellgro) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Primary VSMCs (2x104-3x104) were seeded onto to an Xfe96 poly-D-Lysine (PDL) Cell Culture Plate (Agilent) in 80uL of 5% FBS ...
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bioRxiv - Cell Biology 2023Quote: The oxygen consumption rate in cybrid cell lines was detected using an XFe24 extracellular flux analyzer (Seahorse Bioscience) as described59 with some modifications ...
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS and medium exchanged for Seahorse XF Base Medium Minimal DMEM (Agilent Technologies, #102353) supplied with 10 mM glucose ...
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bioRxiv - Immunology 2024Quote: ... 4×105 treated NK cells were resuspended in Seahorse XF DMEM Medium containing glutamine (1 mM; Agilent, USA) and seeded into an 8-well XF cell culture microplate (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values ranged from 8.2-10 for all samples ...
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bioRxiv - Genetics 2021Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values ranged from 7.3–10 for all samples ...
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bioRxiv - Developmental Biology 2020Quote: ... analysis was performed using TransStart Tip Green qPCR SuperMix kit (TransGen Co.) on a Stratagene Mx3000P real-time PCR system (Stratagene, La Jolla, CA, USA) under the conditions of 94 °C for 30 s ...
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bioRxiv - Genomics 2019Quote: ... and successful size-selection verified by electrophoresis on an Agilent TapeStation using the Genomic DNA High ScreenTape kit (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Standard quality control steps were included to determine total RNA quantity and quality using Agilent 2100 Bioanalyzer with Eukaryote Total RNA Pico Kit and High Sensitivity DNA Assay (Agilent Technologies, Santa Clara, CA). Libraries were prepared using the TruSeq Stranded mRNA Library preparation Kit (Illumina Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... early and late nascent strands were labeled with Cy3 and Cy5 ULS molecules using the Genomic DNA labeling Kit (Agilent, Santa Clara, CA, USA) following manufacturer's instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmid DNA mutations in the predicted orthosteric binding site of PAR4 were generated using the QuikChange XL Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
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bioRxiv - Physiology 2019Quote: ... RNA quality and integrity were validated using a NanoDrop ND-1000 UV–vis Spectrophotometer (NanoDrop Technologies, Wilmington, USA) and Agilent 2100 Bioanalyzer and RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, USA) respectively ...
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bioRxiv - Immunology 2019Quote: Various N-linked glycosylation site mutants of UL144 were generated by site-directed mutagenesis using Quick Change II site directed mutagenesis Kit (Stratagene, La Jolla, CA, USA). The Asn residues carrying the N-linked glycan were mutated individually or in combination at residues N61 ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting supernatants were transferred to new tubes and 50 µl of each cleared lysate was mixed with 50 µl Lysis Buffer (0.7 µl β-mercaptoethanol/100 µl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at –80° for later preparation as input RNA ...
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bioRxiv - Genomics 2021Quote: ... Libraries from all sample sets were quantified on the 2100 Bioanalyzer instrument with the Agilent DNA 7500 Kit (both Agilent Technologies, Santa Clara, CA).
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bioRxiv - Bioengineering 2022Quote: ... Size pattern was assessed with Fragment Analyzer-96 with the DNF-474-0500 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies, DNF-474-0500). Libraries (Average size ...
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bioRxiv - Biochemistry 2021Quote: Mutated forms of rat and human BDH2 enzymes (Y157F and Y147F, respectively) were generated by site-directed mutagenesis using a QuikChange II XL kit (Stratagene, La Jolla, CA, USA), with pCOLD I/rBDH2 and pCOLD I/hBDH2 plasmids as templates and mutagenic primers Y157F-rBDH2-S and Y157F-rBDH2-AS as well as Y147F-hBDH2-S and Y147F-hBDH2-AS ...
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bioRxiv - Neuroscience 2020Quote: ... the purity and concentration of RNA samples were determined by readings of OD260/280 using a dual beam UV spectrophotometer and RNA integrity was determined by capillary electrophoresis using the RNA 6000 Nano Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and Ile-residues of the HXXXDX motif of ZmGL2 and ZmGL2-LIKE were generated using QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The ZmGL2 protein was also expressed in E ...
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bioRxiv - Systems Biology 2020Quote: ... The sub-libraries were each resuspended to a final volume of 20 μL in nuclease-free water and fragment distribution was checked running a bioanalyzer (Agilent High Sensitivity DNA Kit) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2021Quote: ... The fragment sizes and concentrations of the pooled samples were determined on a Fragment analyzer 5200 using the DNF-473-Standard Sensitivity NGS Fragment Analysis Kit (Agilent, Santa Clara, CA, USA). The indexing PCR was performed under the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting supernatants were transferred to new tubes and 50 μl of each cleared lysate was mixed with 50 μl Lysis Buffer (0.7 μl β-mercaptoethanol/100 μl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at −80°C for later preparation as Input RNA ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were pooled in equal quantity and combined to multiplex to make a final library and QC was done again using Qubit quantification kit and Agilent bioanalyzer using high sensitivity DNA chip (Agilent Biotechnology, catalog # 5067-4626). The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using High output 300-bp Single end Read sequencing kit.